O43680 (TCF21_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor 21
Short name=TCF-21

Alternative name(s):
Capsulin
Class A basic helix-loop-helix protein 23
Short name=bHLHa23
Epicardin
Podocyte-expressed 1
Short name=Pod-1

Gene names

Name:	TCF21
Synonyms:	BHLHA23, POD1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types.
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3 and binds the E box (5'-CANNTG-3').
Subcellular location	Nucleus.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Sertoli cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL branchiomeric skeletal muscle development Inferred from sequence or structural similarity. Source: UniProtKB bronchiole development Inferred from sequence or structural similarity. Source: UniProtKB diaphragm development Inferred from sequence or structural similarity. Source: UniProtKB embryonic digestive tract morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB epithelial cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB gland development Inferred from sequence or structural similarity. Source: UniProtKB lung alveolus development Inferred from sequence or structural similarity. Source: UniProtKB lung morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB lung vasculature development Inferred from sequence or structural similarity. Source: UniProtKB metanephric glomerular capillary formation Inferred from sequence or structural similarity. Source: UniProtKB metanephric mesenchymal cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of androgen receptor signaling pathway Inferred from sequence or structural similarity. Source: BHF-UCL palate development Inferred from sequence or structural similarity. Source: UniProtKB sex determination Inferred from sequence or structural similarity. Source: UniProtKB spleen development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay PubMed 12493738. Source: UniProtKB
Molecular_function	E-box binding Inferred from sequence or structural similarity. Source: BHF-UCL RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay PubMed 12493738. Source: UniProtKB RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from sequence or structural similarity. Source: UniProtKB androgen receptor binding Inferred from sequence or structural similarity. Source: BHF-UCL protein dimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Transcription factor 21		PRO_0000127464
Regions
	Domain	79 – 131	53	bHLH
Experimental info
	Sequence conflict	61	1	K → R in AAC62514. Ref.2
	Sequence conflict	83	1	A → T in AAC62532. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O43680 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: A26F496C536B6F04	FASTA	179	19,715
	10 20 30 40 50 60 MSTGSLSDVE DLQEVEMLEC DGLKMDSNKE FVTSNESTEE SSNCENGSPQ KGRGGLGKRR 70 80 90 100 110 120 KAPTKKSPLS GVSQEGKQVQ RNAANARERA RMRVLSKAFS RLKTTLPWVP PDTKLSKLDT 130 140 150 160 170 LRLASSYIAH LRQILANDKY ENGYIHPVNL TWPFMVAGKP ESDLKEVVTA SRLCGTTAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Epicardin: a novel basic helix-loop-helix transcription factor gene expressed in epicardium, branchial arch myoblasts, and mesenchyme of developing lung, gut, kidney, and gonads." Robb L., Mifsud L., Hartley L., Biben C., Copeland N.G., Gilbert D.J., Jenkins N.A., Harvey R.P. Dev. Dyn. 213:105-113(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"The basic-helix-loop-helix protein pod1 is critically important for kidney and lung organogenesis." Quaggin S.E., Schwartz L., Cui S., Igarashi P., Deimling J., Post M., Rossant J. Development 126:5771-5783(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF047419 mRNA. Translation: AAC62532.1. AF035718 mRNA. Translation: AAC62514.1. BT019660 mRNA. Translation: AAV38466.1. CR450293 mRNA. Translation: CAG29289.1. AL356109 Genomic DNA. Translation: CAC26822.1. CH471051 Genomic DNA. Translation: EAW48000.1. CH471051 Genomic DNA. Translation: EAW48001.1. BC025697 mRNA. Translation: AAH25697.1.
IPI	IPI00306587.
RefSeq	NP_003197.2. NM_003206.3. NP_938206.1. NM_198392.2.
UniGene	Hs.78061.
3D structure databases
ProteinModelPortal	O43680.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43680. 6 interactions.
MINT	MINT-1180543.
STRING	9606.ENSP00000237316.
PTM databases
PhosphoSite	O43680.
Proteomic databases
PaxDb	O43680.
PRIDE	O43680.
Protocols and materials databases
DNASU	6943.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000237316; ENSP00000237316; ENSG00000118526. ENST00000367882; ENSP00000356857; ENSG00000118526.
GeneID	6943.
KEGG	hsa:6943.
UCSC	uc003qei.4. human.
Organism-specific databases
CTD	6943.
GeneCards	GC06P134251.
HGNC	HGNC:11632. TCF21.
HPA	HPA013189.
MIM	603306. gene.
neXtProt	NX_O43680.
PharmGKB	PA36387.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG235686.
HOGENOM	HOG000261670.
HOVERGEN	HBG105298.
InParanoid	O43680.
OMA	ESSNCET.
OrthoDB	EOG4BZN40.
Gene expression databases
Bgee	O43680.
CleanEx	HS_TCF21.
Genevestigator	O43680.
GermOnline	ENSG00000118526. Homo sapiens.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. [Graphical view]
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6943.
NextBio	27179.
SOURCE	Search...

Entry information

Entry name

TCF21_HUMAN

Accession

Primary (citable) accession number: O43680
Secondary accession number(s): E1P581 , O43545, Q6ICV0, Q9BZ14

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	August 14, 2001
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.