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O43680 (TCF21_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor 21

Short name=TCF-21
Alternative name(s):
Capsulin
Class A basic helix-loop-helix protein 23
Short name=bHLHa23
Epicardin
Podocyte-expressed 1
Short name=Pod-1
Gene names
Name:TCF21
Synonyms:BHLHA23, POD1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types.

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3 and binds the E box (5'-CANNTG-3').

Subcellular location

Nucleus.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSertoli cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

branching involved in ureteric bud morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

branchiomeric skeletal muscle development

Inferred from sequence or structural similarity. Source: UniProtKB

bronchiole development

Inferred from sequence or structural similarity. Source: UniProtKB

diaphragm development

Inferred from sequence or structural similarity. Source: UniProtKB

embryonic digestive tract morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

epithelial cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

gland development

Inferred from sequence or structural similarity. Source: UniProtKB

glomerulus development

Inferred from sequence or structural similarity. Source: UniProtKB

kidney development

Inferred from sequence or structural similarity. Source: BHF-UCL

lung alveolus development

Inferred from sequence or structural similarity. Source: UniProtKB

lung morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

lung vasculature development

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric glomerular capillary formation

Inferred from sequence or structural similarity. Source: UniProtKB

metanephric mesenchymal cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

morphogenesis of a branching structure

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of androgen receptor signaling pathway

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 12493738. Source: UniProtKB

palate development

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

reproductive structure development

Inferred from sequence or structural similarity. Source: UniProtKB

respiratory system development

Inferred from sequence or structural similarity. Source: UniProtKB

sex determination

Inferred from sequence or structural similarity. Source: UniProtKB

spleen development

Inferred from sequence or structural similarity. Source: UniProtKB

ureteric bud development

Inferred from sequence or structural similarity. Source: BHF-UCL

vasculature development

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentnucleus

Inferred from direct assay PubMed 12493738. Source: UniProtKB

   Molecular_functionE-box binding

Inferred from sequence or structural similarity. Source: BHF-UCL

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from direct assay PubMed 12493738. Source: UniProtKB

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from sequence or structural similarity. Source: UniProtKB

androgen receptor binding

Inferred from sequence or structural similarity. Source: BHF-UCL

bHLH transcription factor binding

Inferred from physical interaction PubMed 12493738. Source: UniProtKB

protein dimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179Transcription factor 21
PRO_0000127464

Regions

Domain79 – 13153bHLH

Experimental info

Sequence conflict611K → R in AAC62514. Ref.2
Sequence conflict831A → T in AAC62532. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O43680 [UniParc].

Last modified August 14, 2001. Version 2.
Checksum: A26F496C536B6F04

FASTA17919,715
        10         20         30         40         50         60 
MSTGSLSDVE DLQEVEMLEC DGLKMDSNKE FVTSNESTEE SSNCENGSPQ KGRGGLGKRR 

        70         80         90        100        110        120 
KAPTKKSPLS GVSQEGKQVQ RNAANARERA RMRVLSKAFS RLKTTLPWVP PDTKLSKLDT 

       130        140        150        160        170 
LRLASSYIAH LRQILANDKY ENGYIHPVNL TWPFMVAGKP ESDLKEVVTA SRLCGTTAS 

« Hide

References

« Hide 'large scale' references
[1]"Epicardin: a novel basic helix-loop-helix transcription factor gene expressed in epicardium, branchial arch myoblasts, and mesenchyme of developing lung, gut, kidney, and gonads."
Robb L., Mifsud L., Hartley L., Biben C., Copeland N.G., Gilbert D.J., Jenkins N.A., Harvey R.P.
Dev. Dyn. 213:105-113(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"The basic-helix-loop-helix protein pod1 is critically important for kidney and lung organogenesis."
Quaggin S.E., Schwartz L., Cui S., Igarashi P., Deimling J., Post M., Rossant J.
Development 126:5771-5783(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF047419 mRNA. Translation: AAC62532.1.
AF035718 mRNA. Translation: AAC62514.1.
BT019660 mRNA. Translation: AAV38466.1.
CR450293 mRNA. Translation: CAG29289.1.
AL356109 Genomic DNA. Translation: CAC26822.1.
CH471051 Genomic DNA. Translation: EAW48000.1.
CH471051 Genomic DNA. Translation: EAW48001.1.
BC025697 mRNA. Translation: AAH25697.1.
RefSeqNP_003197.2. NM_003206.3.
NP_938206.1. NM_198392.2.
UniGeneHs.78061.

3D structure databases

ProteinModelPortalO43680.
SMRO43680. Positions 80-135.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112803. 9 interactions.
IntActO43680. 7 interactions.
MINTMINT-1180543.
STRING9606.ENSP00000237316.

PTM databases

PhosphoSiteO43680.

Proteomic databases

PaxDbO43680.
PRIDEO43680.

Protocols and materials databases

DNASU6943.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000237316; ENSP00000237316; ENSG00000118526.
ENST00000367882; ENSP00000356857; ENSG00000118526.
GeneID6943.
KEGGhsa:6943.
UCSCuc003qei.4. human.

Organism-specific databases

CTD6943.
GeneCardsGC06P134251.
HGNCHGNC:11632. TCF21.
HPAHPA013189.
MIM603306. gene.
neXtProtNX_O43680.
PharmGKBPA36387.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG235686.
HOGENOMHOG000261670.
HOVERGENHBG105298.
InParanoidO43680.
OMAESSNCET.
OrthoDBEOG7J181B.
PhylomeDBO43680.
TreeFamTF350742.

Gene expression databases

BgeeO43680.
CleanExHS_TCF21.
GenevestigatorO43680.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTCF21_(gene).
GenomeRNAi6943.
NextBio27179.
PROO43680.
SOURCESearch...

Entry information

Entry nameTCF21_HUMAN
AccessionPrimary (citable) accession number: O43680
Secondary accession number(s): E1P581 expand/collapse secondary AC list , O43545, Q6ICV0, Q9BZ14
Entry history
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: August 14, 2001
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM