O43674 (NDUB5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

Alternative name(s):
Complex I-SGDH
Short name=CI-SGDH
NADH-ubiquinone oxidoreductase SGDH subunit

Gene names

Name:

NDUFB5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Subunit structure	Complex I is composed of 45 different subunits. Ref.5
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.
Sequence similarities	Belongs to the complex I NDUFB5 subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transit peptide Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, NADH to ubiquinone Non-traceable author statement PubMed 9878551. Source: UniProtKB small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex I Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	NADH dehydrogenase (ubiquinone) activity Non-traceable author statement PubMed 9878551. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43674-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43674-2)

The sequence of this isoform differs from the canonical sequence as follows:
     42-93: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Mitochondrion By similarity
	Chain	47 – 189	143	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial		PRO_0000020052
Regions
	Transmembrane	73 – 93	21	Helical; Potential
Natural variations
	Alternative sequence	42 – 93	52	Missing in isoform 2.		VSP_042516
	Natural variant	133	1	Y → H. Corresponds to variant rs4147793 [ dbSNP | Ensembl ].		VAR_050590

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 66D288F7AB7FD5C5	FASTA	189	21,750
	10 20 30 40 50 60 MAAMSLLRRV SVTAVAALSG RPLGTRLGFG GFLTRGFPKA AAPVRHSGDH GKRLFVIRPS 70 80 90 100 110 120 RFYDRRFLKL LRFYIALTGI PVAIFITLVN VFIGQAELAE IPEGYVPEHW EYYKHPISRW 130 140 150 160 170 180 IARNFYDSPE KIYERTMAVL QIEAEKAELR VKELEVRKLM HVRGDGPWYY YETIDKELID HSPKATPDN
	Isoform 2 [UniParc]. Checksum: CD8C5524D8116C46	FASTA	137	15,667
	10 20 30 40 50 60 MAAMSLLRRV SVTAVAALSG RPLGTRLGFG GFLTRGFPKA AGQAELAEIP EGYVPEHWEY 70 80 90 100 110 120 YKHPISRWIA RNFYDSPEKI YERTMAVLQI EAEKAELRVK ELEVRKLMHV RGDGPWYYYE 130 TIDKELIDHS PKATPDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits." Ton C., Hwang D.M., Dempsey A.A., Liew C.-C. Biochem. Biophys. Res. Commun. 241:589-594(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Heart.
[2]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain, Skin and Urinary bladder.
[5]	"The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification." Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F., Ghosh S.S., Capaldi R.A. J. Biol. Chem. 278:13619-13622(2003) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF047181 mRNA. Translation: AAC04266.1. AC090425 Genomic DNA. No translation available. CH471052 Genomic DNA. Translation: EAW78393.1. BC005271 mRNA. Translation: AAH05271.1. BC093071 mRNA. Translation: AAH93071.1. BC009796 mRNA. Translation: AAH09796.1.
IPI	IPI00013459. IPI00556190.
PIR	JC5820.
RefSeq	NP_001186886.1. NM_001199957.1. NP_001186887.1. NM_001199958.1. NP_002483.1. NM_002492.3.
UniGene	Hs.730674.
3D structure databases
ProteinModelPortal	O43674.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43674. 2 interactions.
STRING	9606.ENSP00000259037.
PTM databases
PhosphoSite	O43674.
Proteomic databases
PaxDb	O43674.
PRIDE	O43674.
Protocols and materials databases
DNASU	4711.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000259037; ENSP00000259037; ENSG00000136521. ENST00000493866; ENSP00000419656; ENSG00000136521.
GeneID	4711.
KEGG	hsa:4711.
UCSC	uc003fkc.3. human.
Organism-specific databases
CTD	4711.
GeneCards	GC03P179322.
HGNC	HGNC:7700. NDUFB5.
HPA	HPA005640.
MIM	603841. gene.
neXtProt	NX_O43674.
PharmGKB	PA31511.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG297432.
HOGENOM	HOG000004849.
HOVERGEN	HBG003008.
InParanoid	O43674.
KO	K03961.
OrthoDB	EOG4THVV6.
PhylomeDB	O43674.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	O43674.
Bgee	O43674.
CleanEx	HS_NDUFB5.
Genevestigator	O43674.
GermOnline	ENSG00000136521. Homo sapiens.
Family and domain databases
InterPro	IPR019173. NADH_UbQ_OxRdtase_B5_su. [Graphical view]
PANTHER	PTHR13178. PTHR13178. 1 hit.
Pfam	PF09781. NDUF_B5. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O43674.
ChEMBL	CHEMBL4337.
ChiTaRS	NDUFB5. human.
DrugBank	DB00157. NADH.
GenomeRNAi	4711.
NextBio	18168.
SOURCE	Search...

Entry information

Entry name

NDUB5_HUMAN

Accession

Primary (citable) accession number: O43674
Secondary accession number(s): Q561V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.