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Protein

Regulator of G-protein signaling 10

Gene

RGS10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the muscarinic acetylcholine receptor CHRM2. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:8774883, PubMed:10608901, PubMed:9353196, PubMed:11443111, PubMed:18434541). Modulates the activity of potassium channels that are activated in response to CHRM2 signaling (PubMed:11443111). Activity on GNAZ is inhibited by palmitoylation of the G-protein (PubMed:9353196).5 Publications

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: UniProtKB
  • GTPase activator activity Source: UniProtKB

GO - Biological processi

  • G-protein coupled acetylcholine receptor signaling pathway Source: UniProtKB
  • negative regulation of signal transduction Source: UniProtKB-KW
  • positive regulation of GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Signal transduction inhibitor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000148908-MONOMER.
ReactomeiR-HSA-418594. G alpha (i) signalling events.
SignaLinkiO43665.
SIGNORiO43665.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 10
Short name:
RGS10
Gene namesi
Name:RGS10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:9992. RGS10.

Subcellular locationi

  • Cytoplasmcytosol 1 Publication
  • Nucleus 1 Publication

  • Note: Forskolin treatment promotes phosphorylation and translocation to the nucleus.1 Publication
Isoform 2 :
  • Nucleus 1 Publication

GO - Cellular componenti

  • axon terminus Source: Ensembl
  • cytosol Source: UniProtKB
  • dendritic spine Source: Ensembl
  • neuronal cell body Source: Ensembl
  • nucleus Source: UniProtKB
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168S → A: Abolishes phosphorylation site and leads to strongly reduced overall phosphorylation. 1 Publication1

Organism-specific databases

DisGeNETi6001.
OpenTargetsiENSG00000148908.
PharmGKBiPA34362.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002042071 – 173Regulator of G-protein signaling 10Add BLAST173

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphoserineBy similarity1
Lipidationi66S-palmitoyl cysteine1 Publication1
Modified residuei168Phosphoserine1 Publication1
Isoform 3 (identifier: O43665-3)
Modified residuei16PhosphoserineCombined sources1

Post-translational modificationi

Isoform 3 is phosphorylated on Ser-16.

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiO43665.
MaxQBiO43665.
PaxDbiO43665.
PeptideAtlasiO43665.
PRIDEiO43665.
TopDownProteomicsiO43665-3. [O43665-3]

2D gel databases

OGPiO43665.

PTM databases

iPTMnetiO43665.
PhosphoSitePlusiO43665.
SwissPalmiO43665.

Miscellaneous databases

PMAP-CutDBO43665.

Expressioni

Gene expression databases

BgeeiENSG00000148908.
CleanExiHS_RGS10.
GenevisibleiO43665. HS.

Organism-specific databases

HPAiCAB004559.
HPA021305.
HPA058485.

Interactioni

Subunit structurei

Interacts with GNAZ, GNAI1 and GNAI3 (PubMed:8774883, PubMed:11443111, PubMed:18434541). Associates specifically with the activated, GTP-bound forms of GNAZ and GNAI3 (PubMed:8774883).3 Publications

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111933. 8 interactors.
DIPiDIP-50368N.
STRINGi9606.ENSP00000358099.

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 32Combined sources8
Helixi34 – 39Combined sources6
Helixi41 – 53Combined sources13
Helixi58 – 71Combined sources14
Helixi75 – 89Combined sources15
Helixi94 – 96Combined sources3
Helixi103 – 105Combined sources3
Helixi108 – 112Combined sources5
Turni116 – 119Combined sources4
Helixi120 – 132Combined sources13
Helixi134 – 138Combined sources5
Turni142 – 144Combined sources3
Turni146 – 150Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DLRNMR-A23-158[»]
2I59NMR-A22-157[»]
2IHBX-ray2.71B9-152[»]
ProteinModelPortaliO43665.
SMRiO43665.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43665.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 148RGSPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000119142.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiO43665.
KOiK16449.
OMAiPKSTAKW.
OrthoDBiEOG091G0BDG.
PhylomeDBiO43665.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43665-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQSELCFADI HDSDGSSSSS HQSLKSTAKW AASLENLLED PEGVKRFREF
60 70 80 90 100
LKKEFSEENV LFWLACEDFK KMQDKTQMQE KAKEIYMTFL SSKASSQVNV
110 120 130 140 150
EGQSRLNEKI LEEPHPLMFQ KLQDQIFNLM KYDSYSRFLK SDLFLKHKRT
160 170
EEEEEDLPDA QTAAKRASRI YNT
Length:173
Mass (Da):20,236
Last modified:August 14, 2001 - v2
Checksum:iCA73D1E38F551EF1
GO
Isoform 2 (identifier: O43665-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MQSELCFAD → MEH

Show »
Length:167
Mass (Da):19,608
Checksum:iEF36915F1AA23F3B
GO
Isoform 3 (identifier: O43665-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MQSELCFA → MFNRAVSRLSRKRPPS

Show »
Length:181
Mass (Da):21,210
Checksum:iD83CCB98A6EE2182
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01189694A → V.Corresponds to variant rs1802228dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0056811 – 9MQSELCFAD → MEH in isoform 2. 2 Publications9
Alternative sequenceiVSP_0273661 – 8MQSELCFA → MFNRAVSRLSRKRPPS in isoform 3. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF368902 mRNA. Translation: AAK52979.1.
AF045229 mRNA. Translation: AAC03783.1.
AF493934 mRNA. Translation: AAM12648.1.
AK290773 mRNA. Translation: BAF83462.1.
CR457008 mRNA. Translation: CAG33289.1.
AL355273, AC012468 Genomic DNA. Translation: CAI16486.1.
AL355273 Genomic DNA. Translation: CAI16488.1.
CH471066 Genomic DNA. Translation: EAW49389.1.
CH471066 Genomic DNA. Translation: EAW49390.1.
BC009361 mRNA. Translation: AAH09361.1.
CCDSiCCDS31294.1. [O43665-3]
CCDS41572.1. [O43665-2]
PIRiS71812.
RefSeqiNP_001005339.1. NM_001005339.1. [O43665-3]
NP_002916.1. NM_002925.3. [O43665-2]
UniGeneiHs.501200.

Genome annotation databases

EnsembliENST00000369101; ENSP00000358097; ENSG00000148908. [O43665-1]
ENST00000369103; ENSP00000358099; ENSG00000148908. [O43665-3]
ENST00000392865; ENSP00000376605; ENSG00000148908. [O43665-2]
GeneIDi6001.
KEGGihsa:6001.
UCSCiuc001lee.4. human. [O43665-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF368902 mRNA. Translation: AAK52979.1.
AF045229 mRNA. Translation: AAC03783.1.
AF493934 mRNA. Translation: AAM12648.1.
AK290773 mRNA. Translation: BAF83462.1.
CR457008 mRNA. Translation: CAG33289.1.
AL355273, AC012468 Genomic DNA. Translation: CAI16486.1.
AL355273 Genomic DNA. Translation: CAI16488.1.
CH471066 Genomic DNA. Translation: EAW49389.1.
CH471066 Genomic DNA. Translation: EAW49390.1.
BC009361 mRNA. Translation: AAH09361.1.
CCDSiCCDS31294.1. [O43665-3]
CCDS41572.1. [O43665-2]
PIRiS71812.
RefSeqiNP_001005339.1. NM_001005339.1. [O43665-3]
NP_002916.1. NM_002925.3. [O43665-2]
UniGeneiHs.501200.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DLRNMR-A23-158[»]
2I59NMR-A22-157[»]
2IHBX-ray2.71B9-152[»]
ProteinModelPortaliO43665.
SMRiO43665.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111933. 8 interactors.
DIPiDIP-50368N.
STRINGi9606.ENSP00000358099.

PTM databases

iPTMnetiO43665.
PhosphoSitePlusiO43665.
SwissPalmiO43665.

2D gel databases

OGPiO43665.

Proteomic databases

EPDiO43665.
MaxQBiO43665.
PaxDbiO43665.
PeptideAtlasiO43665.
PRIDEiO43665.
TopDownProteomicsiO43665-3. [O43665-3]

Protocols and materials databases

DNASUi6001.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369101; ENSP00000358097; ENSG00000148908. [O43665-1]
ENST00000369103; ENSP00000358099; ENSG00000148908. [O43665-3]
ENST00000392865; ENSP00000376605; ENSG00000148908. [O43665-2]
GeneIDi6001.
KEGGihsa:6001.
UCSCiuc001lee.4. human. [O43665-1]

Organism-specific databases

CTDi6001.
DisGeNETi6001.
GeneCardsiRGS10.
HGNCiHGNC:9992. RGS10.
HPAiCAB004559.
HPA021305.
HPA058485.
MIMi602856. gene.
neXtProtiNX_O43665.
OpenTargetsiENSG00000148908.
PharmGKBiPA34362.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000119142.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiO43665.
KOiK16449.
OMAiPKSTAKW.
OrthoDBiEOG091G0BDG.
PhylomeDBiO43665.
TreeFamiTF315837.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000148908-MONOMER.
ReactomeiR-HSA-418594. G alpha (i) signalling events.
SignaLinkiO43665.
SIGNORiO43665.

Miscellaneous databases

ChiTaRSiRGS10. human.
EvolutionaryTraceiO43665.
GeneWikiiRGS10.
GenomeRNAii6001.
PMAP-CutDBO43665.
PROiO43665.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148908.
CleanExiHS_RGS10.
GenevisibleiO43665. HS.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGS10_HUMAN
AccessioniPrimary (citable) accession number: O43665
Secondary accession number(s): A8K408
, B1AMR8, Q6IAZ6, Q96GN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 14, 2001
Last modified: November 2, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.