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O43660

- PLRG1_HUMAN

UniProt

O43660 - PLRG1_HUMAN

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Protein

Pleiotropic regulator 1

Gene

PLRG1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.By similarity

GO - Molecular functioni

  1. signal transducer activity Source: ProtInc
  2. transcription corepressor activity Source: ProtInc

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: UniProtKB
  2. positive regulation of G1/S transition of mitotic cell cycle Source: Ensembl
  3. protein localization to nucleus Source: Ensembl
  4. regulation of RNA biosynthetic process Source: GOC
  5. signal transduction Source: GOC
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiREACT_467. mRNA Splicing - Major Pathway.
SignaLinkiO43660.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleiotropic regulator 1
Gene namesi
Name:PLRG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:9089. PLRG1.

Subcellular locationi

GO - Cellular componenti

  1. catalytic step 2 spliceosome Source: UniProtKB
  2. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33416.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Pleiotropic regulator 1PRO_0000051133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO43660.
PaxDbiO43660.
PRIDEiO43660.

PTM databases

PhosphoSiteiO43660.

Expressioni

Gene expression databases

BgeeiO43660.
CleanExiHS_PLRG1.
ExpressionAtlasiO43660. baseline and differential.
GenevestigatoriO43660.

Organism-specific databases

HPAiHPA035931.
HPA035932.

Interactioni

Subunit structurei

Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its WD40 repeat domain) directly with CDC5L (via its C-terminal); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts directly in the complex with BCAS2 and PRPF19 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5LQ994592EBI-1051504,EBI-374880
HNRNPMP522725EBI-1051504,EBI-486809

Protein-protein interaction databases

BioGridi111370. 43 interactions.
IntActiO43660. 15 interactions.
MINTiMINT-2999551.
STRINGi9606.ENSP00000303191.

Structurei

3D structure databases

ProteinModelPortaliO43660.
SMRiO43660. Positions 150-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati202 – 24140WD 1Add
BLAST
Repeati244 – 28340WD 2Add
BLAST
Repeati286 – 32540WD 3Add
BLAST
Repeati328 – 36740WD 4Add
BLAST
Repeati370 – 41041WD 5Add
BLAST
Repeati411 – 44939WD 6Add
BLAST
Repeati460 – 49940WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat PRL1/PRL2 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00520000055597.
HOGENOMiHOG000207595.
HOVERGENiHBG053621.
InParanoidiO43660.
KOiK12862.
OrthoDBiEOG7R2BJC.
PhylomeDBiO43660.
TreeFamiTF105684.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O43660-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN
60 70 80 90 100
EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQEV EYFVAGTHPY
110 120 130 140 150
PPGPGVALTA DTKIQRMPSE SAAQSLAVAL PLQTKADANR TAPSGSEYRH
160 170 180 190 200
PGASDRPQPT AMNSIVMETG NTKNSALMAK KAPTMPKPQW HPPWKLYRVI
210 220 230 240 250
SGHLGWVRCI AVEPGNQWFV TGSADRTIKI WDLASGKLKL SLTGHISTVR
260 270 280 290 300
GVIVSTRSPY LFSCGEDKQV KCWDLEYNKV IRHYHGHLSA VYGLDLHPTI
310 320 330 340 350
DVLVTCSRDS TARIWDVRTK ASVHTLSGHT NAVATVRCQA AEPQIITGSH
360 370 380 390 400
DTTIRLWDLV AGKTRVTLTN HKKSVRAVVL HPRHYTFASG SPDNIKQWKF
410 420 430 440 450
PDGSFIQNLS GHNAIINTLT VNSDGVLVSG ADNGTMHLWD WRTGYNFQRV
460 470 480 490 500
HAAVQPGSLD SESGIFACAF DQSESRLLTA EADKTIKVYR EDDTATEETH
510
PVSWKPEIIK RKRF
Length:514
Mass (Da):57,194
Last modified:June 1, 1998 - v1
Checksum:i8C914CAF5F7887BD
GO
Isoform 2 (identifier: O43660-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-104: Missing.

Note: May be due to a competing donor splice site. No experimental confirmation available.

Show »
Length:505
Mass (Da):56,290
Checksum:i65F7E135A4026411
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei96 – 1049Missing in isoform 2. 1 PublicationVSP_008804

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044333 mRNA. Translation: AAD09407.1.
AK002108 mRNA. Translation: BAG51016.1.
CH471056 Genomic DNA. Translation: EAX04941.1.
BC020786 mRNA. Translation: AAH20786.1.
BC106004 mRNA. Translation: AAI06005.1.
CCDSiCCDS34083.1. [O43660-1]
CCDS56341.1. [O43660-2]
RefSeqiNP_001188493.1. NM_001201564.1. [O43660-2]
NP_002660.1. NM_002669.3. [O43660-1]
UniGeneiHs.249996.

Genome annotation databases

EnsembliENST00000302078; ENSP00000303191; ENSG00000171566. [O43660-2]
ENST00000499023; ENSP00000424417; ENSG00000171566. [O43660-1]
GeneIDi5356.
KEGGihsa:5356.
UCSCiuc003iny.3. human. [O43660-1]
uc003inz.3. human. [O43660-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044333 mRNA. Translation: AAD09407.1 .
AK002108 mRNA. Translation: BAG51016.1 .
CH471056 Genomic DNA. Translation: EAX04941.1 .
BC020786 mRNA. Translation: AAH20786.1 .
BC106004 mRNA. Translation: AAI06005.1 .
CCDSi CCDS34083.1. [O43660-1 ]
CCDS56341.1. [O43660-2 ]
RefSeqi NP_001188493.1. NM_001201564.1. [O43660-2 ]
NP_002660.1. NM_002669.3. [O43660-1 ]
UniGenei Hs.249996.

3D structure databases

ProteinModelPortali O43660.
SMRi O43660. Positions 150-489.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111370. 43 interactions.
IntActi O43660. 15 interactions.
MINTi MINT-2999551.
STRINGi 9606.ENSP00000303191.

PTM databases

PhosphoSitei O43660.

Proteomic databases

MaxQBi O43660.
PaxDbi O43660.
PRIDEi O43660.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000302078 ; ENSP00000303191 ; ENSG00000171566 . [O43660-2 ]
ENST00000499023 ; ENSP00000424417 ; ENSG00000171566 . [O43660-1 ]
GeneIDi 5356.
KEGGi hsa:5356.
UCSCi uc003iny.3. human. [O43660-1 ]
uc003inz.3. human. [O43660-2 ]

Organism-specific databases

CTDi 5356.
GeneCardsi GC04M155457.
HGNCi HGNC:9089. PLRG1.
HPAi HPA035931.
HPA035932.
MIMi 605961. gene.
neXtProti NX_O43660.
PharmGKBi PA33416.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00520000055597.
HOGENOMi HOG000207595.
HOVERGENi HBG053621.
InParanoidi O43660.
KOi K12862.
OrthoDBi EOG7R2BJC.
PhylomeDBi O43660.
TreeFami TF105684.

Enzyme and pathway databases

Reactomei REACT_467. mRNA Splicing - Major Pathway.
SignaLinki O43660.

Miscellaneous databases

GeneWikii PLRG1.
GenomeRNAii 5356.
NextBioi 20762.
PROi O43660.
SOURCEi Search...

Gene expression databases

Bgeei O43660.
CleanExi HS_PLRG1.
ExpressionAtlasi O43660. baseline and differential.
Genevestigatori O43660.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 7 hits.
[Graphical view ]
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Lung.
  5. "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry."
    Ajuh P., Kuster B., Panov K., Zomerdijk J.C.B.M., Mann M., Lamond A.I.
    EMBO J. 19:6569-6581(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 8-41; 48-62; 69-113; 117-135; 141-180; 199-226; 238-250; 258-268; 283-308; 321-355; 377-396; 443-449 AND 488-510, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CDC5L AND THE SPLICEOSOME.
  6. "A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing."
    Ajuh P., Sleeman J., Chusainow J., Lamond A.I.
    J. Biol. Chem. 276:42370-42381(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L AND THE SPLICEOSOME.
  7. "Large-scale proteomic analysis of the human spliceosome."
    Rappsilber J., Ryder U., Lamond A.I., Mann M.
    Genome Res. 12:1231-1245(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 81-113 AND 450-476, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH THE SPLICEOSOME.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION AS A COMPONENT OF THE PRP19-CDC5L SPLICING COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L; PRPF19 AND BCAS2.

Entry informationi

Entry nameiPLRG1_HUMAN
AccessioniPrimary (citable) accession number: O43660
Secondary accession number(s): B3KMK4, Q3KQY5, Q8WUD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3