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Protein

Pleiotropic regulator 1

Gene

PLRG1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.By similarity

GO - Molecular functioni

  1. signal transducer activity Source: ProtInc
  2. transcription corepressor activity Source: ProtInc

GO - Biological processi

  1. gene expression Source: Reactome
  2. mRNA splicing, via spliceosome Source: UniProtKB
  3. negative regulation of nucleic acid-templated transcription Source: GOC
  4. positive regulation of G1/S transition of mitotic cell cycle Source: Ensembl
  5. protein localization to nucleus Source: Ensembl
  6. RNA splicing Source: Reactome
  7. signal transduction Source: GOC
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiREACT_467. mRNA Splicing - Major Pathway.
SignaLinkiO43660.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleiotropic regulator 1
Gene namesi
Name:PLRG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:9089. PLRG1.

Subcellular locationi

GO - Cellular componenti

  1. catalytic step 2 spliceosome Source: UniProtKB
  2. nuclear speck Source: UniProtKB-SubCell
  3. nucleoplasm Source: HPA
  4. nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33416.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Pleiotropic regulator 1PRO_0000051133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO43660.
PaxDbiO43660.
PRIDEiO43660.

PTM databases

PhosphoSiteiO43660.

Expressioni

Gene expression databases

BgeeiO43660.
CleanExiHS_PLRG1.
ExpressionAtlasiO43660. baseline and differential.
GenevestigatoriO43660.

Organism-specific databases

HPAiHPA035931.
HPA035932.

Interactioni

Subunit structurei

Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its WD40 repeat domain) directly with CDC5L (via its C-terminal); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts directly in the complex with BCAS2 and PRPF19 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5LQ994592EBI-1051504,EBI-374880
HNRNPMP522725EBI-1051504,EBI-486809

Protein-protein interaction databases

BioGridi111370. 43 interactions.
IntActiO43660. 15 interactions.
MINTiMINT-2999551.
STRINGi9606.ENSP00000303191.

Structurei

3D structure databases

ProteinModelPortaliO43660.
SMRiO43660. Positions 150-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati202 – 24140WD 1Add
BLAST
Repeati244 – 28340WD 2Add
BLAST
Repeati286 – 32540WD 3Add
BLAST
Repeati328 – 36740WD 4Add
BLAST
Repeati370 – 41041WD 5Add
BLAST
Repeati411 – 44939WD 6Add
BLAST
Repeati460 – 49940WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat PRL1/PRL2 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00780000121928.
HOGENOMiHOG000207595.
HOVERGENiHBG053621.
InParanoidiO43660.
KOiK12862.
OrthoDBiEOG7R2BJC.
PhylomeDBiO43660.
TreeFamiTF105684.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43660-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN
60 70 80 90 100
EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQEV EYFVAGTHPY
110 120 130 140 150
PPGPGVALTA DTKIQRMPSE SAAQSLAVAL PLQTKADANR TAPSGSEYRH
160 170 180 190 200
PGASDRPQPT AMNSIVMETG NTKNSALMAK KAPTMPKPQW HPPWKLYRVI
210 220 230 240 250
SGHLGWVRCI AVEPGNQWFV TGSADRTIKI WDLASGKLKL SLTGHISTVR
260 270 280 290 300
GVIVSTRSPY LFSCGEDKQV KCWDLEYNKV IRHYHGHLSA VYGLDLHPTI
310 320 330 340 350
DVLVTCSRDS TARIWDVRTK ASVHTLSGHT NAVATVRCQA AEPQIITGSH
360 370 380 390 400
DTTIRLWDLV AGKTRVTLTN HKKSVRAVVL HPRHYTFASG SPDNIKQWKF
410 420 430 440 450
PDGSFIQNLS GHNAIINTLT VNSDGVLVSG ADNGTMHLWD WRTGYNFQRV
460 470 480 490 500
HAAVQPGSLD SESGIFACAF DQSESRLLTA EADKTIKVYR EDDTATEETH
510
PVSWKPEIIK RKRF
Length:514
Mass (Da):57,194
Last modified:May 31, 1998 - v1
Checksum:i8C914CAF5F7887BD
GO
Isoform 2 (identifier: O43660-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-104: Missing.

Note: May be due to a competing donor splice site. No experimental confirmation available.

Show »
Length:505
Mass (Da):56,290
Checksum:i65F7E135A4026411
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei96 – 1049Missing in isoform 2. 1 PublicationVSP_008804

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044333 mRNA. Translation: AAD09407.1.
AK002108 mRNA. Translation: BAG51016.1.
CH471056 Genomic DNA. Translation: EAX04941.1.
BC020786 mRNA. Translation: AAH20786.1.
BC106004 mRNA. Translation: AAI06005.1.
CCDSiCCDS34083.1. [O43660-1]
CCDS56341.1. [O43660-2]
RefSeqiNP_001188493.1. NM_001201564.1. [O43660-2]
NP_002660.1. NM_002669.3. [O43660-1]
UniGeneiHs.249996.

Genome annotation databases

EnsembliENST00000302078; ENSP00000303191; ENSG00000171566. [O43660-2]
ENST00000499023; ENSP00000424417; ENSG00000171566. [O43660-1]
GeneIDi5356.
KEGGihsa:5356.
UCSCiuc003iny.3. human. [O43660-1]
uc003inz.3. human. [O43660-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044333 mRNA. Translation: AAD09407.1.
AK002108 mRNA. Translation: BAG51016.1.
CH471056 Genomic DNA. Translation: EAX04941.1.
BC020786 mRNA. Translation: AAH20786.1.
BC106004 mRNA. Translation: AAI06005.1.
CCDSiCCDS34083.1. [O43660-1]
CCDS56341.1. [O43660-2]
RefSeqiNP_001188493.1. NM_001201564.1. [O43660-2]
NP_002660.1. NM_002669.3. [O43660-1]
UniGeneiHs.249996.

3D structure databases

ProteinModelPortaliO43660.
SMRiO43660. Positions 150-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111370. 43 interactions.
IntActiO43660. 15 interactions.
MINTiMINT-2999551.
STRINGi9606.ENSP00000303191.

PTM databases

PhosphoSiteiO43660.

Proteomic databases

MaxQBiO43660.
PaxDbiO43660.
PRIDEiO43660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302078; ENSP00000303191; ENSG00000171566. [O43660-2]
ENST00000499023; ENSP00000424417; ENSG00000171566. [O43660-1]
GeneIDi5356.
KEGGihsa:5356.
UCSCiuc003iny.3. human. [O43660-1]
uc003inz.3. human. [O43660-2]

Organism-specific databases

CTDi5356.
GeneCardsiGC04M155457.
HGNCiHGNC:9089. PLRG1.
HPAiHPA035931.
HPA035932.
MIMi605961. gene.
neXtProtiNX_O43660.
PharmGKBiPA33416.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00780000121928.
HOGENOMiHOG000207595.
HOVERGENiHBG053621.
InParanoidiO43660.
KOiK12862.
OrthoDBiEOG7R2BJC.
PhylomeDBiO43660.
TreeFamiTF105684.

Enzyme and pathway databases

ReactomeiREACT_467. mRNA Splicing - Major Pathway.
SignaLinkiO43660.

Miscellaneous databases

GeneWikiiPLRG1.
GenomeRNAii5356.
NextBioi20762.
PROiO43660.
SOURCEiSearch...

Gene expression databases

BgeeiO43660.
CleanExiHS_PLRG1.
ExpressionAtlasiO43660. baseline and differential.
GenevestigatoriO43660.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Lung.
  5. "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry."
    Ajuh P., Kuster B., Panov K., Zomerdijk J.C.B.M., Mann M., Lamond A.I.
    EMBO J. 19:6569-6581(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 8-41; 48-62; 69-113; 117-135; 141-180; 199-226; 238-250; 258-268; 283-308; 321-355; 377-396; 443-449 AND 488-510, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CDC5L AND THE SPLICEOSOME.
  6. "A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing."
    Ajuh P., Sleeman J., Chusainow J., Lamond A.I.
    J. Biol. Chem. 276:42370-42381(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L AND THE SPLICEOSOME.
  7. "Large-scale proteomic analysis of the human spliceosome."
    Rappsilber J., Ryder U., Lamond A.I., Mann M.
    Genome Res. 12:1231-1245(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 81-113 AND 450-476, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH THE SPLICEOSOME.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION AS A COMPONENT OF THE PRP19-CDC5L SPLICING COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L; PRPF19 AND BCAS2.

Entry informationi

Entry nameiPLRG1_HUMAN
AccessioniPrimary (citable) accession number: O43660
Secondary accession number(s): B3KMK4, Q3KQY5, Q8WUD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 6, 2003
Last sequence update: May 31, 1998
Last modified: March 3, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.