O43660 (PLRG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pleiotropic regulator 1

Gene names

Name:

PLRG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing By similarity. Ref.6
Subunit structure	Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its WD40 repeat domain) directly with CDC5L (via its C-terminal); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts directly in the complex with BCAS2 and PRPF19 By similarity. Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus. Nucleus speckle Ref.6 Ref.8.
Sequence similarities	Belongs to the WD repeat PRL1/PRL2 family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred by curator PubMed 11991638. Source: UniProtKB
Cellular_component	catalytic step 2 spliceosome Inferred from direct assay PubMed 11991638. Source: UniProtKB nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	signal transducer activity Traceable author statement Ref.1. Source: ProtInc transcription corepressor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43660-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43660-2)

The sequence of this isoform differs from the canonical sequence as follows:
     96-104: Missing.

Note: May be due to a competing donor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	Pleiotropic regulator 1		PRO_0000051133
Regions
	Repeat	202 – 241	40	WD 1
	Repeat	244 – 283	40	WD 2
	Repeat	286 – 325	40	WD 3
	Repeat	328 – 367	40	WD 4
	Repeat	370 – 410	41	WD 5
	Repeat	411 – 449	39	WD 6
	Repeat	460 – 499	40	WD 7
Natural variations
	Alternative sequence	96 – 104	9	Missing in isoform 2.		VSP_008804

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 8C914CAF5F7887BD	FASTA	514	57,194
	10 20 30 40 50 60 MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT 70 80 90 100 110 120 SKENLKEKGP QNATDSYVHK QYPANQGQEV EYFVAGTHPY PPGPGVALTA DTKIQRMPSE 130 140 150 160 170 180 SAAQSLAVAL PLQTKADANR TAPSGSEYRH PGASDRPQPT AMNSIVMETG NTKNSALMAK 190 200 210 220 230 240 KAPTMPKPQW HPPWKLYRVI SGHLGWVRCI AVEPGNQWFV TGSADRTIKI WDLASGKLKL 250 260 270 280 290 300 SLTGHISTVR GVIVSTRSPY LFSCGEDKQV KCWDLEYNKV IRHYHGHLSA VYGLDLHPTI 310 320 330 340 350 360 DVLVTCSRDS TARIWDVRTK ASVHTLSGHT NAVATVRCQA AEPQIITGSH DTTIRLWDLV 370 380 390 400 410 420 AGKTRVTLTN HKKSVRAVVL HPRHYTFASG SPDNIKQWKF PDGSFIQNLS GHNAIINTLT 430 440 450 460 470 480 VNSDGVLVSG ADNGTMHLWD WRTGYNFQRV HAAVQPGSLD SESGIFACAF DQSESRLLTA 490 500 510 EADKTIKVYR EDDTATEETH PVSWKPEIIK RKRF
	Isoform 2 [UniParc]. Checksum: 65F7E135A4026411	FASTA	505	56,290
	10 20 30 40 50 60 MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT 70 80 90 100 110 120 SKENLKEKGP QNATDSYVHK QYPANQGQEV EYFVAGVALT ADTKIQRMPS ESAAQSLAVA 130 140 150 160 170 180 LPLQTKADAN RTAPSGSEYR HPGASDRPQP TAMNSIVMET GNTKNSALMA KKAPTMPKPQ 190 200 210 220 230 240 WHPPWKLYRV ISGHLGWVRC IAVEPGNQWF VTGSADRTIK IWDLASGKLK LSLTGHISTV 250 260 270 280 290 300 RGVIVSTRSP YLFSCGEDKQ VKCWDLEYNK VIRHYHGHLS AVYGLDLHPT IDVLVTCSRD 310 320 330 340 350 360 STARIWDVRT KASVHTLSGH TNAVATVRCQ AAEPQIITGS HDTTIRLWDL VAGKTRVTLT 370 380 390 400 410 420 NHKKSVRAVV LHPRHYTFAS GSPDNIKQWK FPDGSFIQNL SGHNAIINTL TVNSDGVLVS 430 440 450 460 470 480 GADNGTMHLW DWRTGYNFQR VHAAVQPGSL DSESGIFACA FDQSESRLLT AEADKTIKVY 490 500 REDDTATEET HPVSWKPEII KRKRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in Arabidopsis." Nemeth K., Salchert K., Putnoky P., Bhalerao R., Koncz-Kalman Z., Stankovic-Stangeland B., Bako L., Mathur J., Oekresz L., Stabel S., Geigenberger P., Stitt M., Redei G.P., Schell J., Koncz C. Genes Dev. 12:3059-3073(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[5]	"Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry." Ajuh P., Kuster B., Panov K., Zomerdijk J.C.B.M., Mann M., Lamond A.I. EMBO J. 19:6569-6581(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 8-41; 48-62; 69-113; 117-135; 141-180; 199-226; 238-250; 258-268; 283-308; 321-355; 377-396; 443-449 AND 488-510, MASS SPECTROMETRY, INTERACTION WITH CDC5L AND THE SPLICEOSOME.
[6]	"A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing." Ajuh P., Sleeman J., Chusainow J., Lamond A.I. J. Biol. Chem. 276:42370-42381(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L AND THE SPLICEOSOME.
[7]	"Large-scale proteomic analysis of the human spliceosome." Rappsilber J., Ryder U., Lamond A.I., Mann M. Genome Res. 12:1231-1245(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 81-113 AND 450-476, MASS SPECTROMETRY, INTERACTION WITH THE SPLICEOSOME.
[8]	"Molecular architecture of the human Prp19/CDC5L complex." Grote M., Wolf E., Will C.L., Lemm I., Agafonov D.E., Schomburg A., Fischle W., Urlaub H., Luhrmann R. Mol. Cell. Biol. 30:2105-2119(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A COMPONENT OF THE PRP19-CDC5L SPLICING COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L; PRPF19 AND BCAS2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF044333 mRNA. Translation: AAD09407.1. AK002108 mRNA. Translation: BAG51016.1. CH471056 Genomic DNA. Translation: EAX04941.1. BC020786 mRNA. Translation: AAH20786.1. BC106004 mRNA. Translation: AAI06005.1.
IPI	IPI00002624. IPI00382756.
RefSeq	NP_001188493.1. NM_001201564.1. NP_002660.1. NM_002669.3.
UniGene	Hs.249996.
3D structure databases
ProteinModelPortal	O43660.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43660. 8 interactions.
MINT	MINT-2999551.
STRING	9606.ENSP00000303191.
PTM databases
PhosphoSite	O43660.
Proteomic databases
PaxDb	O43660.
PRIDE	O43660.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000302078; ENSP00000303191; ENSG00000171566. ENST00000499023; ENSP00000424417; ENSG00000171566.
GeneID	5356.
KEGG	hsa:5356.
UCSC	uc003iny.3. human. uc003inz.3. human.
Organism-specific databases
CTD	5356.
GeneCards	GC04M155457.
HGNC	HGNC:9089. PLRG1.
HPA	HPA035932.
MIM	605961. gene.
neXtProt	NX_O43660.
PharmGKB	PA33416.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000207595.
HOVERGEN	HBG053621.
KO	K12862.
OrthoDB	EOG4M65HB.
PhylomeDB	O43660.
Gene expression databases
ArrayExpress	O43660.
Bgee	O43660.
CleanEx	HS_PLRG1.
Genevestigator	O43660.
GermOnline	ENSG00000171566. Homo sapiens.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 7 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5356.
NextBio	20762.
SOURCE	Search...

Entry information

Entry name

PLRG1_HUMAN

Accession

Primary (citable) accession number: O43660
Secondary accession number(s): B3KMK4, Q3KQY5, Q8WUD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.