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O43660

- PLRG1_HUMAN

UniProt

O43660 - PLRG1_HUMAN

Protein

Pleiotropic regulator 1

Gene

PLRG1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.By similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. signal transducer activity Source: ProtInc
    3. transcription corepressor activity Source: ProtInc

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: UniProtKB
    2. regulation of RNA biosynthetic process Source: GOC
    3. signal transduction Source: GOC

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    SignaLinkiO43660.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pleiotropic regulator 1
    Gene namesi
    Name:PLRG1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:9089. PLRG1.

    Subcellular locationi

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB
    2. nuclear speck Source: UniProtKB-SubCell
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33416.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 514514Pleiotropic regulator 1PRO_0000051133Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO43660.
    PaxDbiO43660.
    PRIDEiO43660.

    PTM databases

    PhosphoSiteiO43660.

    Expressioni

    Gene expression databases

    ArrayExpressiO43660.
    BgeeiO43660.
    CleanExiHS_PLRG1.
    GenevestigatoriO43660.

    Organism-specific databases

    HPAiHPA035931.
    HPA035932.

    Interactioni

    Subunit structurei

    Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its WD40 repeat domain) directly with CDC5L (via its C-terminal); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts directly in the complex with BCAS2 and PRPF19 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC5LQ994592EBI-1051504,EBI-374880
    HNRNPMP522725EBI-1051504,EBI-486809

    Protein-protein interaction databases

    BioGridi111370. 42 interactions.
    IntActiO43660. 15 interactions.
    MINTiMINT-2999551.
    STRINGi9606.ENSP00000303191.

    Structurei

    3D structure databases

    ProteinModelPortaliO43660.
    SMRiO43660. Positions 150-489.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati202 – 24140WD 1Add
    BLAST
    Repeati244 – 28340WD 2Add
    BLAST
    Repeati286 – 32540WD 3Add
    BLAST
    Repeati328 – 36740WD 4Add
    BLAST
    Repeati370 – 41041WD 5Add
    BLAST
    Repeati411 – 44939WD 6Add
    BLAST
    Repeati460 – 49940WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat PRL1/PRL2 family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000207595.
    HOVERGENiHBG053621.
    KOiK12862.
    OrthoDBiEOG7R2BJC.
    PhylomeDBiO43660.
    TreeFamiTF105684.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 7 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O43660-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN    50
    EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQEV EYFVAGTHPY 100
    PPGPGVALTA DTKIQRMPSE SAAQSLAVAL PLQTKADANR TAPSGSEYRH 150
    PGASDRPQPT AMNSIVMETG NTKNSALMAK KAPTMPKPQW HPPWKLYRVI 200
    SGHLGWVRCI AVEPGNQWFV TGSADRTIKI WDLASGKLKL SLTGHISTVR 250
    GVIVSTRSPY LFSCGEDKQV KCWDLEYNKV IRHYHGHLSA VYGLDLHPTI 300
    DVLVTCSRDS TARIWDVRTK ASVHTLSGHT NAVATVRCQA AEPQIITGSH 350
    DTTIRLWDLV AGKTRVTLTN HKKSVRAVVL HPRHYTFASG SPDNIKQWKF 400
    PDGSFIQNLS GHNAIINTLT VNSDGVLVSG ADNGTMHLWD WRTGYNFQRV 450
    HAAVQPGSLD SESGIFACAF DQSESRLLTA EADKTIKVYR EDDTATEETH 500
    PVSWKPEIIK RKRF 514
    Length:514
    Mass (Da):57,194
    Last modified:June 1, 1998 - v1
    Checksum:i8C914CAF5F7887BD
    GO
    Isoform 2 (identifier: O43660-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         96-104: Missing.

    Note: May be due to a competing donor splice site. No experimental confirmation available.

    Show »
    Length:505
    Mass (Da):56,290
    Checksum:i65F7E135A4026411
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei96 – 1049Missing in isoform 2. 1 PublicationVSP_008804

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044333 mRNA. Translation: AAD09407.1.
    AK002108 mRNA. Translation: BAG51016.1.
    CH471056 Genomic DNA. Translation: EAX04941.1.
    BC020786 mRNA. Translation: AAH20786.1.
    BC106004 mRNA. Translation: AAI06005.1.
    CCDSiCCDS34083.1. [O43660-1]
    CCDS56341.1. [O43660-2]
    RefSeqiNP_001188493.1. NM_001201564.1. [O43660-2]
    NP_002660.1. NM_002669.3. [O43660-1]
    UniGeneiHs.249996.

    Genome annotation databases

    EnsembliENST00000302078; ENSP00000303191; ENSG00000171566. [O43660-2]
    ENST00000499023; ENSP00000424417; ENSG00000171566. [O43660-1]
    GeneIDi5356.
    KEGGihsa:5356.
    UCSCiuc003iny.3. human. [O43660-1]
    uc003inz.3. human. [O43660-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044333 mRNA. Translation: AAD09407.1 .
    AK002108 mRNA. Translation: BAG51016.1 .
    CH471056 Genomic DNA. Translation: EAX04941.1 .
    BC020786 mRNA. Translation: AAH20786.1 .
    BC106004 mRNA. Translation: AAI06005.1 .
    CCDSi CCDS34083.1. [O43660-1 ]
    CCDS56341.1. [O43660-2 ]
    RefSeqi NP_001188493.1. NM_001201564.1. [O43660-2 ]
    NP_002660.1. NM_002669.3. [O43660-1 ]
    UniGenei Hs.249996.

    3D structure databases

    ProteinModelPortali O43660.
    SMRi O43660. Positions 150-489.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111370. 42 interactions.
    IntActi O43660. 15 interactions.
    MINTi MINT-2999551.
    STRINGi 9606.ENSP00000303191.

    PTM databases

    PhosphoSitei O43660.

    Proteomic databases

    MaxQBi O43660.
    PaxDbi O43660.
    PRIDEi O43660.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000302078 ; ENSP00000303191 ; ENSG00000171566 . [O43660-2 ]
    ENST00000499023 ; ENSP00000424417 ; ENSG00000171566 . [O43660-1 ]
    GeneIDi 5356.
    KEGGi hsa:5356.
    UCSCi uc003iny.3. human. [O43660-1 ]
    uc003inz.3. human. [O43660-2 ]

    Organism-specific databases

    CTDi 5356.
    GeneCardsi GC04M155457.
    HGNCi HGNC:9089. PLRG1.
    HPAi HPA035931.
    HPA035932.
    MIMi 605961. gene.
    neXtProti NX_O43660.
    PharmGKBi PA33416.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000207595.
    HOVERGENi HBG053621.
    KOi K12862.
    OrthoDBi EOG7R2BJC.
    PhylomeDBi O43660.
    TreeFami TF105684.

    Enzyme and pathway databases

    SignaLinki O43660.

    Miscellaneous databases

    GeneWikii PLRG1.
    GenomeRNAii 5356.
    NextBioi 20762.
    PROi O43660.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O43660.
    Bgeei O43660.
    CleanExi HS_PLRG1.
    Genevestigatori O43660.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 7 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Lung.
    5. "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry."
      Ajuh P., Kuster B., Panov K., Zomerdijk J.C.B.M., Mann M., Lamond A.I.
      EMBO J. 19:6569-6581(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 8-41; 48-62; 69-113; 117-135; 141-180; 199-226; 238-250; 258-268; 283-308; 321-355; 377-396; 443-449 AND 488-510, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH CDC5L AND THE SPLICEOSOME.
    6. "A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing."
      Ajuh P., Sleeman J., Chusainow J., Lamond A.I.
      J. Biol. Chem. 276:42370-42381(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L AND THE SPLICEOSOME.
    7. "Large-scale proteomic analysis of the human spliceosome."
      Rappsilber J., Ryder U., Lamond A.I., Mann M.
      Genome Res. 12:1231-1245(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 81-113 AND 450-476, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH THE SPLICEOSOME.
    8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION AS A COMPONENT OF THE PRP19-CDC5L SPLICING COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L; PRPF19 AND BCAS2.

    Entry informationi

    Entry nameiPLRG1_HUMAN
    AccessioniPrimary (citable) accession number: O43660
    Secondary accession number(s): B3KMK4, Q3KQY5, Q8WUD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 7, 2003
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3