O43617 (TPPC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trafficking protein particle complex subunit 3

Alternative name(s):
BET3 homolog

Gene names

Name:	TRAPPC3
Synonyms:	BET3
ORF Names:	CDABP0066

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in vesicular transport from endoplasmic reticulum to Golgi.
Subunit structure	Homodimer. Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Heterodimer with TRAPPC6A. The heterodimer TRAPPC3-TRAPPC6A interacts with TRAPPC2L. Ref.7 Ref.8 Ref.10 Ref.11
Subcellular location	Golgi apparatus › cis-Golgi network By similarity. Endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the TRAPP small subunits family. BET3 subfamily.

Ontologies

Keywords
Biological process	ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus
PTM	Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA Golgi membrane Inferred from electronic annotation. Source: Compara TRAPP complex Inferred from electronic annotation. Source: Compara cytosol Inferred from electronic annotation. Source: Compara endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 180

180

Trafficking protein particle complex subunit 3

PRO_0000211572

Amino acid modifications

Lipidation

68

1

S-palmitoyl cysteine Ref.11

Experimental info

Mutagenesis

68

1

C → S: Loss of palmitoylation. Ref.11

Secondary structure

1

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180

Helix Strand Turn

Details...

Helix

17 – 34

18

Helix

37 – 62

26

Helix

71 – 80

10

Helix

82 – 87

6

Beta strand

92 – 94

3

Beta strand

102 – 109

8

Helix

120 – 122

3

Turn

127 – 129

3

Helix

130 – 140

11

Turn

141 – 143

3

Beta strand

144 – 152

9

Helix

154 – 156

3

Beta strand

159 – 170

12

Sequences

	Sequence		Length	Mass (Da)
	O43617 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: B5E2D92BE8A39599	FASTA	180	20,274
	10 20 30 40 50 60 MSRQANRGTE SKKMSSELFT LTYGALVTQL CKDYENDEDV NKQLDKMGFN IGVRLIEDFL 70 80 90 100 110 120 ARSNVGRCHD FRETADVIAK VAFKMYLGIT PSITNWSPAG DEFSLILENN PLVDFVELPD 130 140 150 160 170 180 NHSSLIYSNL LCGVLRGALE MVQMAVEAKF VQDTLKGDGV TEIRMRFIRR IEDNLPAGEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion." Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S. EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	Eva L., Subramaniam V.N., Hong W. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Pediatric leukemia cDNA sequencing project." Zhou J., Yu W., Tang H., Mei G., Tsang Y.T.M., Bouck J., Gibbs R.A., Margolin J.F. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Leukemia.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[7]	"Functional organization of the yeast proteome by systematic analysis of protein complexes." Gavin A.-C., Boesche M., Krause R., Grandi P., Marzioch M., Bauer A., Schultz J., Rick J.M., Michon A.-M., Cruciat C.-M., Remor M., Hoefert C., Schelder M., Brajenovic M., Ruffner H., Merino A., Klein K., Hudak M. , Dickson D., Rudi T., Gnau V., Bauch A., Bastuck S., Huhse B., Leutwein C., Heurtier M.-A., Copley R.R., Edelmann A., Querfurth E., Rybin V., Drewes G., Raida M., Bouwmeester T., Bork P., Seraphin B., Kuster B., Neubauer G., Superti-Furga G. Nature 415:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN TRAPP COMPLEX.
[8]	"TRAPPC2L is a novel, highly conserved TRAPP-interacting protein." Scrivens P.J., Shahrzad N., Moores A., Morin A., Brunet S., Sacher M. Traffic 10:724-736(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN TRAPP COMPLEX, INTERACTION WITH TRAPPC2L.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"C4orf41 and TTC-15 are mammalian TRAPP components with a role at an early stage in ER-to-Golgi trafficking." Scrivens P.J., Noueihed B., Shahrzad N., Hul S., Brunet S., Sacher M. Mol. Biol. Cell 22:2083-2093(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN TRAPP COMPLEX.
[11]	"Structure of palmitoylated BET3: insights into TRAPP complex assembly and membrane localization." Turnbull A.P., Kummel D., Prinz B., Holz C., Schultchen J., Lang C., Niesen F.H., Hofmann K.P., Delbruck H., Behlke J., Muller E.C., Jarosch E., Sommer T., Heinemann U. EMBO J. 24:875-884(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 2-180, SUBUNIT, MUTAGENESIS OF CYS-68, PALMITOYLATION AT CYS-68.
[12]	"Biochemical and crystallographic studies reveal a specific interaction between TRAPP subunits Trs33p and Bet3p." Kim M.-S., Yi M.-J., Lee K.-H., Wagner J., Munger C., Kim Y.-G., Whiteway M., Cygler M., Oh B.-H., Sacher M. Traffic 6:1183-1195(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 15-175 IN COMPLEX WITH TRAPPC6A.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ224335 mRNA. Translation: CAA11902.1.
AF041432 mRNA. Translation: AAB96936.1.
AY007139 mRNA. Translation: AAG02000.1.
AK315610 mRNA. Translation: BAG37979.1.
CH471059 Genomic DNA. Translation: EAX07386.1.
CH471059 Genomic DNA. Translation: EAX07387.1.
BC007662 mRNA. Translation: AAH07662.1.

IPI

IPI00004324.

RefSeq

NP_001257823.1. NM_001270894.1.
NP_001257824.1. NM_001270895.1.
NP_001257825.1. NM_001270896.1.
NP_001257826.1. NM_001270897.1.
NP_055223.1. NM_014408.4.

UniGene

Hs.523131.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SZ7	X-ray	1.55	A	2-180	[»]
2C0J	X-ray	2.20	A	15-175	[»]
2CFH	X-ray	2.30	A/B	1-180	[»]
3KXC	X-ray	2.00	A	1-180	[»]

ProteinModelPortal

O43617.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47627N.

IntAct

O43617. 5 interactions.

MINT

MINT-5002244.

STRING

9606.ENSP00000362261.

PTM databases

PhosphoSite

O43617.

Proteomic databases

PaxDb

O43617.

PRIDE

O43617.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000373166; ENSP00000362261; ENSG00000054116.

GeneID

27095.

KEGG

hsa:27095.

UCSC

uc001bzx.3. human.

Organism-specific databases

CTD

27095.

GeneCards

GC01M036602.

HGNC

HGNC:19942. TRAPPC3.

HPA

HPA028408.

MIM

610955. gene.

neXtProt

NX_O43617.

PharmGKB

PA134972272.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG286899.

HOGENOM

HOG000186184.

HOVERGEN

HBG055045.

InParanoid

O43617.

OMA

WFVQDQL.

OrthoDB

EOG42FSJP.

PhylomeDB

O43617.

Gene expression databases

ArrayExpress

O43617.

Bgee

O43617.

CleanEx

HS_TRAPPC3.

Genevestigator

O43617.

GermOnline

ENSG00000054116. Homo sapiens.

Family and domain databases

InterPro

IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR007194. TRAPP_component.
IPR016721. TRAPP_I_complex_Bet3.
[Graphical view]

PANTHER

PTHR13048. PTHR13048. 1 hit.

Pfam

PF04051. TRAPP. 1 hit.
[Graphical view]

PIRSF

PIRSF018293. TRAPP_I_complex_Bet3. 1 hit.

SUPFAM

SSF111126. Haem_NO-bd. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

TRAPPC3. human.

EvolutionaryTrace

O43617.

GenomeRNAi

27095.

NextBio

49737.

SOURCE

Search...

Entry information

Entry name

TPPC3_HUMAN

Accession

Primary (citable) accession number: O43617
Secondary accession number(s): B2RDN2, D3DPS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.