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Protein

Mitochondrial import inner membrane translocase subunit TIM44

Gene

TIMM44

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 173ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • protein import into mitochondrial matrix Source: GO_Central
  • protein targeting to mitochondrion Source: ProtInc

Keywordsi

Biological processProtein transport, Translocation, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1268020 Mitochondrial protein import

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM44
Gene namesi
Name:TIMM44
Synonyms:MIMT44, TIM44
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000104980.7
HGNCiHGNC:17316 TIMM44
MIMi605058 gene
neXtProtiNX_O43615

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10469
OpenTargetsiENSG00000104980
PharmGKBiPA38229

Polymorphism and mutation databases

BioMutaiTIMM44

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000034314? – 452Mitochondrial import inner membrane translocase subunit TIM44
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128PhosphothreonineCombined sources1
Modified residuei180PhosphoserineCombined sources1
Modified residuei217N6-succinyllysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO43615
MaxQBiO43615
PaxDbiO43615
PeptideAtlasiO43615
PRIDEiO43615

PTM databases

iPTMnetiO43615
PhosphoSitePlusiO43615
SwissPalmiO43615

Expressioni

Gene expression databases

BgeeiENSG00000104980
CleanExiHS_TIMM44
ExpressionAtlasiO43615 baseline and differential
GenevisibleiO43615 HS

Organism-specific databases

HPAiHPA043052

Interactioni

Subunit structurei

Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19 (By similarity). The complex interacts with the TIMM23 component of the TIM23 complex.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115732, 49 interactors
CORUMiO43615
IntActiO43615, 8 interactors
MINTiO43615
STRINGi9606.ENSP00000270538

Structurei

Secondary structure

1452
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi271 – 286Combined sources16
Helixi292 – 306Combined sources15
Helixi312 – 321Combined sources10
Helixi323 – 333Combined sources11
Helixi336 – 342Combined sources7
Helixi345 – 360Combined sources16
Beta strandi368 – 383Combined sources16
Beta strandi386 – 398Combined sources13
Beta strandi400 – 402Combined sources3
Beta strandi408 – 411Combined sources4
Beta strandi417 – 427Combined sources11
Helixi434 – 436Combined sources3
Beta strandi438 – 447Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CW9X-ray1.90A266-452[»]
ProteinModelPortaliO43615
SMRiO43615
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43615

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim44 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2580 Eukaryota
ENOG410Y5IK LUCA
GeneTreeiENSGT00390000000051
HOGENOMiHOG000013004
HOVERGENiHBG055113
InParanoidiO43615
KOiK17804
OMAiETRGWRL
OrthoDBiEOG091G073E
PhylomeDBiO43615
TreeFamiTF106197

Family and domain databases

InterProiView protein in InterPro
IPR032710 NTF2-like_dom_sf
IPR017303 Tim44
IPR007379 Tim44-like_dom
PfamiView protein in Pfam
PF04280 Tim44, 1 hit
PIRSFiPIRSF037871 TIM44, 1 hit
SMARTiView protein in SMART
SM00978 Tim44, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
TIGRFAMsiTIGR00984 3a0801s03tim44, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAALRSGW CRCPRRCLGS GIQFLSSHNL PHGSTYQMRR PGGELPLSKS
60 70 80 90 100
YSSGNRKGFL SGLLDNVKQE LAKNKEMKES IKKFRDEARR LEESDVLQEA
110 120 130 140 150
RRKYKTIESE TVRTSEVLRK KLGELTGTVK ESLHEVSKSD LGRKIKEGVE
160 170 180 190 200
EAAKTAKQSA ESVSKGGEKL GRTAAFRALS QGVESVKKEI DDSVLGQTGP
210 220 230 240 250
YRRPQRLRKR TEFAGDKFKE EKVFEPNEEA LGVVLHKDSK WYQQWKDFKE
260 270 280 290 300
NNVVFNRFFE MKMKYDESDN AFIRASRALT DKVTDLLGGL FSKTEMSEVL
310 320 330 340 350
TEILRVDPAF DKDRFLKQCE NDIIPNVLEA MISGELDILK DWCYEATYSQ
360 370 380 390 400
LAHPIQQAKA LGLQFHSRIL DIDNVDLAMG KMMEQGPVLI ITFQAQLVMV
410 420 430 440 450
VRNPKGEVVE GDPDKVLRML YVWALCRDQD ELNPYAAWRL LDISASSTEQ

IL
Length:452
Mass (Da):51,356
Last modified:November 8, 2002 - v2
Checksum:i15BECF875611BE96
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189E → A in AAB97740 (PubMed:10339406).Curated1
Sequence conflicti226P → A in AAB97740 (PubMed:10339406).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041254 mRNA Translation: AAB97740.1
AK289634 mRNA Translation: BAF82323.1
CH471139 Genomic DNA Translation: EAW68954.1
CH471139 Genomic DNA Translation: EAW68955.1
BC033628 mRNA Translation: AAH33628.1
CCDSiCCDS12192.1
RefSeqiNP_006342.2, NM_006351.3
UniGeneiHs.465784

Genome annotation databases

EnsembliENST00000270538; ENSP00000270538; ENSG00000104980
GeneIDi10469
KEGGihsa:10469
UCSCiuc002miz.4 human

Similar proteinsi

Entry informationi

Entry nameiTIM44_HUMAN
AccessioniPrimary (citable) accession number: O43615
Secondary accession number(s): A8K0R9, D6W664, Q8N193
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 8, 2002
Last modified: April 25, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health