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O43615

- TIM44_HUMAN

UniProt

O43615 - TIM44_HUMAN

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Protein

Mitochondrial import inner membrane translocase subunit TIM44

Gene

TIMM44

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi166 – 1738ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. protein targeting to mitochondrion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_118595. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM44
Gene namesi
Name:TIMM44
Synonyms:MIMT44, TIM44
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:17316. TIMM44.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: BHF-UCL
  2. mitochondrial matrix Source: BHF-UCL
  3. mitochondrion Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38229.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 452Mitochondrial import inner membrane translocase subunit TIM44PRO_0000034314
Transit peptidei1 – ?MitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801Phosphoserine1 Publication
Modified residuei217 – 2171N6-succinyllysineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO43615.
PaxDbiO43615.
PeptideAtlasiO43615.
PRIDEiO43615.

PTM databases

PhosphoSiteiO43615.

Expressioni

Gene expression databases

BgeeiO43615.
CleanExiHS_TIMM44.
ExpressionAtlasiO43615. baseline and differential.
GenevestigatoriO43615.

Organism-specific databases

HPAiHPA043052.

Interactioni

Subunit structurei

Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19 (By similarity). The complex interacts with the TIMM23 component of the TIM23 complex.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ARAFP103983EBI-861737,EBI-365961

Protein-protein interaction databases

BioGridi115732. 26 interactions.
IntActiO43615. 4 interactions.
MINTiMINT-2999483.
STRINGi9606.ENSP00000270538.

Structurei

Secondary structure

1
452
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi271 – 28616
Helixi292 – 30615
Helixi312 – 32110
Helixi323 – 33311
Helixi336 – 3427
Helixi345 – 36016
Beta strandi368 – 38316
Beta strandi386 – 39813
Beta strandi400 – 4023
Beta strandi408 – 4114
Beta strandi417 – 42711
Helixi434 – 4363
Beta strandi438 – 44710

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CW9X-ray1.90A266-452[»]
ProteinModelPortaliO43615.
SMRiO43615. Positions 270-451.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43615.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim44 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG240138.
GeneTreeiENSGT00390000000051.
HOGENOMiHOG000013004.
HOVERGENiHBG055113.
InParanoidiO43615.
KOiK17804.
OMAiKQCENDI.
PhylomeDBiO43615.
TreeFamiTF106197.

Family and domain databases

InterProiIPR017303. Tim44.
IPR007379. Tim44-like_dom.
[Graphical view]
PfamiPF04280. Tim44. 1 hit.
[Graphical view]
PIRSFiPIRSF037871. TIM44. 1 hit.
SMARTiSM00978. Tim44. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00984. 3a0801s03tim44. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43615-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAALRSGW CRCPRRCLGS GIQFLSSHNL PHGSTYQMRR PGGELPLSKS
60 70 80 90 100
YSSGNRKGFL SGLLDNVKQE LAKNKEMKES IKKFRDEARR LEESDVLQEA
110 120 130 140 150
RRKYKTIESE TVRTSEVLRK KLGELTGTVK ESLHEVSKSD LGRKIKEGVE
160 170 180 190 200
EAAKTAKQSA ESVSKGGEKL GRTAAFRALS QGVESVKKEI DDSVLGQTGP
210 220 230 240 250
YRRPQRLRKR TEFAGDKFKE EKVFEPNEEA LGVVLHKDSK WYQQWKDFKE
260 270 280 290 300
NNVVFNRFFE MKMKYDESDN AFIRASRALT DKVTDLLGGL FSKTEMSEVL
310 320 330 340 350
TEILRVDPAF DKDRFLKQCE NDIIPNVLEA MISGELDILK DWCYEATYSQ
360 370 380 390 400
LAHPIQQAKA LGLQFHSRIL DIDNVDLAMG KMMEQGPVLI ITFQAQLVMV
410 420 430 440 450
VRNPKGEVVE GDPDKVLRML YVWALCRDQD ELNPYAAWRL LDISASSTEQ

IL
Length:452
Mass (Da):51,356
Last modified:November 8, 2002 - v2
Checksum:i15BECF875611BE96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti189 – 1891E → A in AAB97740. (PubMed:10339406)Curated
Sequence conflicti226 – 2261P → A in AAB97740. (PubMed:10339406)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041254 mRNA. Translation: AAB97740.1.
AK289634 mRNA. Translation: BAF82323.1.
CH471139 Genomic DNA. Translation: EAW68954.1.
CH471139 Genomic DNA. Translation: EAW68955.1.
BC033628 mRNA. Translation: AAH33628.1.
CCDSiCCDS12192.1.
RefSeqiNP_006342.2. NM_006351.3.
UniGeneiHs.465784.

Genome annotation databases

EnsembliENST00000270538; ENSP00000270538; ENSG00000104980.
GeneIDi10469.
KEGGihsa:10469.
UCSCiuc002miz.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041254 mRNA. Translation: AAB97740.1 .
AK289634 mRNA. Translation: BAF82323.1 .
CH471139 Genomic DNA. Translation: EAW68954.1 .
CH471139 Genomic DNA. Translation: EAW68955.1 .
BC033628 mRNA. Translation: AAH33628.1 .
CCDSi CCDS12192.1.
RefSeqi NP_006342.2. NM_006351.3.
UniGenei Hs.465784.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CW9 X-ray 1.90 A 266-452 [» ]
ProteinModelPortali O43615.
SMRi O43615. Positions 270-451.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115732. 26 interactions.
IntActi O43615. 4 interactions.
MINTi MINT-2999483.
STRINGi 9606.ENSP00000270538.

PTM databases

PhosphoSitei O43615.

Proteomic databases

MaxQBi O43615.
PaxDbi O43615.
PeptideAtlasi O43615.
PRIDEi O43615.

Protocols and materials databases

DNASUi 10469.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000270538 ; ENSP00000270538 ; ENSG00000104980 .
GeneIDi 10469.
KEGGi hsa:10469.
UCSCi uc002miz.3. human.

Organism-specific databases

CTDi 10469.
GeneCardsi GC19M007991.
HGNCi HGNC:17316. TIMM44.
HPAi HPA043052.
MIMi 605058. gene.
neXtProti NX_O43615.
PharmGKBi PA38229.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG240138.
GeneTreei ENSGT00390000000051.
HOGENOMi HOG000013004.
HOVERGENi HBG055113.
InParanoidi O43615.
KOi K17804.
OMAi KQCENDI.
PhylomeDBi O43615.
TreeFami TF106197.

Enzyme and pathway databases

Reactomei REACT_118595. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTracei O43615.
GeneWikii TIMM44.
GenomeRNAii 10469.
NextBioi 39705.
PROi O43615.
SOURCEi Search...

Gene expression databases

Bgeei O43615.
CleanExi HS_TIMM44.
ExpressionAtlasi O43615. baseline and differential.
Genevestigatori O43615.

Family and domain databases

InterProi IPR017303. Tim44.
IPR007379. Tim44-like_dom.
[Graphical view ]
Pfami PF04280. Tim44. 1 hit.
[Graphical view ]
PIRSFi PIRSF037871. TIM44. 1 hit.
SMARTi SM00978. Tim44. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00984. 3a0801s03tim44. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic and structural characterization of the human mitochondrial inner membrane translocase."
    Bauer M.F., Gempel K., Reichert A.S., Rappold G.A., Lichtner P., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S.
    J. Mol. Biol. 289:69-82(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Amygdala.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTIM44_HUMAN
AccessioniPrimary (citable) accession number: O43615
Secondary accession number(s): A8K0R9, D6W664, Q8N193
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 8, 2002
Last modified: October 29, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3