O43615 (TIM44_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM44

Gene names

Name:	TIMM44
Synonyms:	MIMT44, TIM44

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
Subunit structure	Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19 By similarity. The complex interacts with the TIMM23 component of the TIM23 complex.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the Tim44 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein metabolic process Traceable author statement. Source: Reactome protein targeting to mitochondrion Traceable author statement. Source: Reactome
Cellular_component	mitochondrial inner membrane Inferred from direct assay Ref.1. Source: BHF-UCL mitochondrial matrix Inferred from direct assay Ref.1. Source: BHF-UCL
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARAF	P10398	3	EBI-861737,EBI-365961

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 452

Mitochondrial import inner membrane translocase subunit TIM44

PRO_0000034314

Regions

Nucleotide binding

166 – 173

8

ATP Potential

Amino acid modifications

Modified residue

180

1

Phosphoserine Ref.6

Experimental info

Sequence conflict

189

1

E → A in AAB97740. Ref.1

Sequence conflict

226

1

P → A in AAB97740. Ref.1

Secondary structure

1

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452

Helix Strand Turn

Details...

Helix

271 – 286

16

Helix

292 – 306

15

Helix

312 – 321

10

Helix

323 – 333

11

Helix

336 – 342

7

Helix

345 – 360

16

Beta strand

368 – 383

16

Beta strand

386 – 398

13

Beta strand

400 – 402

3

Beta strand

408 – 411

4

Beta strand

417 – 427

11

Helix

434 – 436

3

Beta strand

438 – 447

10

Sequences

	Sequence		Length	Mass (Da)
	O43615 [UniParc]. Last modified November 8, 2002. Version 2. Checksum: 15BECF875611BE96	FASTA	452	51,356
	10 20 30 40 50 60 MAAAALRSGW CRCPRRCLGS GIQFLSSHNL PHGSTYQMRR PGGELPLSKS YSSGNRKGFL 70 80 90 100 110 120 SGLLDNVKQE LAKNKEMKES IKKFRDEARR LEESDVLQEA RRKYKTIESE TVRTSEVLRK 130 140 150 160 170 180 KLGELTGTVK ESLHEVSKSD LGRKIKEGVE EAAKTAKQSA ESVSKGGEKL GRTAAFRALS 190 200 210 220 230 240 QGVESVKKEI DDSVLGQTGP YRRPQRLRKR TEFAGDKFKE EKVFEPNEEA LGVVLHKDSK 250 260 270 280 290 300 WYQQWKDFKE NNVVFNRFFE MKMKYDESDN AFIRASRALT DKVTDLLGGL FSKTEMSEVL 310 320 330 340 350 360 TEILRVDPAF DKDRFLKQCE NDIIPNVLEA MISGELDILK DWCYEATYSQ LAHPIQQAKA 370 380 390 400 410 420 LGLQFHSRIL DIDNVDLAMG KMMEQGPVLI ITFQAQLVMV VRNPKGEVVE GDPDKVLRML 430 440 450 YVWALCRDQD ELNPYAAWRL LDISASSTEQ IL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic and structural characterization of the human mitochondrial inner membrane translocase." Bauer M.F., Gempel K., Reichert A.S., Rappold G.A., Lichtner P., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S. J. Mol. Biol. 289:69-82(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF041254 mRNA. Translation: AAB97740.1.
AK289634 mRNA. Translation: BAF82323.1.
CH471139 Genomic DNA. Translation: EAW68954.1.
CH471139 Genomic DNA. Translation: EAW68955.1.
BC033628 mRNA. Translation: AAH33628.1.

IPI

IPI00306516.

RefSeq

NP_006342.2. NM_006351.3.

UniGene

Hs.465784.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CW9	X-ray	1.90	A	266-452	[»]

ProteinModelPortal

O43615.

ModBase

Search...

Protein-protein interaction databases

IntAct

O43615. 3 interactions.

MINT

MINT-2999483.

STRING

9606.ENSP00000270538.

PTM databases

PhosphoSite

O43615.

Proteomic databases

PaxDb

O43615.

PeptideAtlas

O43615.

PRIDE

O43615.

Protocols and materials databases

DNASU

10469.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000270538; ENSP00000270538; ENSG00000104980.

GeneID

10469.

KEGG

hsa:10469.

UCSC

uc002miz.3. human.

Organism-specific databases

CTD

10469.

GeneCards

GC19M007991.

HGNC

HGNC:17316. TIMM44.

HPA

HPA043052.

MIM

605058. gene.

neXtProt

NX_O43615.

PharmGKB

PA38229.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG240138.

HOGENOM

HOG000013004.

HOVERGEN

HBG055113.

InParanoid

O43615.

OMA

QQCENDI.

OrthoDB

EOG49CQ7J.

PhylomeDB

O43615.

Enzyme and pathway databases

Reactome

REACT_17015. Metabolism of proteins.

Gene expression databases

ArrayExpress

O43615.

Bgee

O43615.

CleanEx

HS_TIMM44.

Genevestigator

O43615.

GermOnline

ENSG00000104980. Homo sapiens.

Family and domain databases

InterPro

IPR017303. Tim44.
IPR007379. Tim44-like_dom.
[Graphical view]

Pfam

PF04280. Tim44. 1 hit.
[Graphical view]

PIRSF

PIRSF037871. TIM44. 1 hit.

SMART

SM00978. Tim44. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00984. 3a0801s03tim44. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

O43615.

GenomeRNAi

10469.

NextBio

39705.

SOURCE

Search...

Entry information

Entry name

TIM44_HUMAN

Accession

Primary (citable) accession number: O43615
Secondary accession number(s): A8K0R9, D6W664, Q8N193

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 8, 2002
Last modified:	May 29, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.