Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O43615 (TIM44_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM44
Gene names
Name:TIMM44
Synonyms:MIMT44, TIM44
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.

Subunit structure

Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19 By similarity. The complex interacts with the TIMM23 component of the TIM23 complex.

Subcellular location

Mitochondrion inner membrane.

Sequence similarities

Belongs to the Tim44 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ARAFP103983EBI-861737,EBI-365961

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 452Mitochondrial import inner membrane translocase subunit TIM44PRO_0000034314

Regions

Nucleotide binding166 – 1738ATP Potential

Amino acid modifications

Modified residue1801Phosphoserine Ref.6
Modified residue2171N6-succinyllysine By similarity

Experimental info

Sequence conflict1891E → A in AAB97740. Ref.1
Sequence conflict2261P → A in AAB97740. Ref.1

Secondary structure

........................... 452
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O43615 [UniParc].

Last modified November 8, 2002. Version 2.
Checksum: 15BECF875611BE96

FASTA45251,356
        10         20         30         40         50         60 
MAAAALRSGW CRCPRRCLGS GIQFLSSHNL PHGSTYQMRR PGGELPLSKS YSSGNRKGFL 

        70         80         90        100        110        120 
SGLLDNVKQE LAKNKEMKES IKKFRDEARR LEESDVLQEA RRKYKTIESE TVRTSEVLRK 

       130        140        150        160        170        180 
KLGELTGTVK ESLHEVSKSD LGRKIKEGVE EAAKTAKQSA ESVSKGGEKL GRTAAFRALS 

       190        200        210        220        230        240 
QGVESVKKEI DDSVLGQTGP YRRPQRLRKR TEFAGDKFKE EKVFEPNEEA LGVVLHKDSK 

       250        260        270        280        290        300 
WYQQWKDFKE NNVVFNRFFE MKMKYDESDN AFIRASRALT DKVTDLLGGL FSKTEMSEVL 

       310        320        330        340        350        360 
TEILRVDPAF DKDRFLKQCE NDIIPNVLEA MISGELDILK DWCYEATYSQ LAHPIQQAKA 

       370        380        390        400        410        420 
LGLQFHSRIL DIDNVDLAMG KMMEQGPVLI ITFQAQLVMV VRNPKGEVVE GDPDKVLRML 

       430        440        450 
YVWALCRDQD ELNPYAAWRL LDISASSTEQ IL 

« Hide

References

« Hide 'large scale' references
[1]"Genetic and structural characterization of the human mitochondrial inner membrane translocase."
Bauer M.F., Gempel K., Reichert A.S., Rappold G.A., Lichtner P., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S.
J. Mol. Biol. 289:69-82(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skeletal muscle.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF041254 mRNA. Translation: AAB97740.1.
AK289634 mRNA. Translation: BAF82323.1.
CH471139 Genomic DNA. Translation: EAW68954.1.
CH471139 Genomic DNA. Translation: EAW68955.1.
BC033628 mRNA. Translation: AAH33628.1.
RefSeqNP_006342.2. NM_006351.3.
UniGeneHs.465784.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CW9X-ray1.90A266-452[»]
ProteinModelPortalO43615.
SMRO43615. Positions 270-451.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115732. 24 interactions.
IntActO43615. 4 interactions.
MINTMINT-2999483.
STRING9606.ENSP00000270538.

PTM databases

PhosphoSiteO43615.

Proteomic databases

PaxDbO43615.
PeptideAtlasO43615.
PRIDEO43615.

Protocols and materials databases

DNASU10469.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000270538; ENSP00000270538; ENSG00000104980.
GeneID10469.
KEGGhsa:10469.
UCSCuc002miz.3. human.

Organism-specific databases

CTD10469.
GeneCardsGC19M007991.
HGNCHGNC:17316. TIMM44.
HPAHPA043052.
MIM605058. gene.
neXtProtNX_O43615.
PharmGKBPA38229.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG240138.
HOGENOMHOG000013004.
HOVERGENHBG055113.
InParanoidO43615.
KOK17804.
OMAKQCENDI.
PhylomeDBO43615.
TreeFamTF106197.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.

Gene expression databases

ArrayExpressO43615.
BgeeO43615.
CleanExHS_TIMM44.
GenevestigatorO43615.

Family and domain databases

InterProIPR017303. Tim44.
IPR007379. Tim44-like_dom.
[Graphical view]
PfamPF04280. Tim44. 1 hit.
[Graphical view]
PIRSFPIRSF037871. TIM44. 1 hit.
SMARTSM00978. Tim44. 1 hit.
[Graphical view]
TIGRFAMsTIGR00984. 3a0801s03tim44. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO43615.
GeneWikiTIMM44.
GenomeRNAi10469.
NextBio39705.
PROO43615.
SOURCESearch...

Entry information

Entry nameTIM44_HUMAN
AccessionPrimary (citable) accession number: O43615
Secondary accession number(s): A8K0R9, D6W664, Q8N193
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 8, 2002
Last modified: April 16, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM