O43609 (SPY1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein sprouty homolog 1
Short name=Spry-1

Gene names

Name:

SPRY1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Note: Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).
Induction	By FGF signaling.
Domain	The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Sequence similarities	Belongs to the sprouty family. Contains 1 SPR (sprouty) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	epidermal growth factor receptor signaling pathway Traceable author statement. Source: Reactome negative regulation of epidermal growth factor receptor signaling pathway Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome lamellipodium Non-traceable author statement. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Protein sprouty homolog 1		PRO_0000076898
Regions
	Domain	183 – 295	113	SPR
	Compositional bias	181 – 310	130	Cys-rich
Amino acid modifications
	Modified residue	53	1	Phosphotyrosine Ref.6 Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	O43609 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 40E2D4D12816E057	FASTA	319	35,122
	10 20 30 40 50 60 MDPQNQHGSG SSLVVIQQPS LDSRQRLDYE REIQPTAILS LDQIKAIRGS NEYTEGPSVV 70 80 90 100 110 120 KRPAPRTAPR QEKHERTHEI IPINVNNNYE HRHTSHLGHA VLPSNARGPI LSRSTSTGSA 130 140 150 160 170 180 ASSGSNSSAS SEQGLLGRSP PTRPVPGHRS ERAIRTQPKQ LIVDDLKGSL KEDLTQHKFI 190 200 210 220 230 240 CEQCGKCKCG ECTAPRTLPS CLACNRQCLC SAESMVEYGT CMCLVKGIFY HCSNDDEGDS 250 260 270 280 290 300 YSDNPCSCSQ SHCCSRYLCM GAMSLFLPCL LCYPPAKGCL KLCRRCYDWI HRPGCRCKNS 310 NTVYCKLESC PSRGQGKPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and expression analysis of a novel splice variant of human Sprouty1 gene." Wang L., Ji C., Wu H., Xu J., Wu Q., Dai J., Yin G., Ye X., Gu S., Xie Y., Mao Y. Int. J. Mol. Med. 12:783-787(2003) [PubMed: 14533010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS.
[4]	Raya A., de la Luna S. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-319.
[5]	"Sprouty encodes a novel antagonist of FGF signaling that patterns apical branching of the Drosophila airways." Hacohen N., Kramer S., Sutherland D., Hiromi Y., Krasnow M.A. Cell 92:253-263(1998) [PubMed: 9458049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-319.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-53, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[7]	"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-53, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[8]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-53, MASS SPECTROMETRY. Tissue: Mammary epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY192146 mRNA. Translation: AAP20102.1. CH471056 Genomic DNA. Translation: EAX05209.1. CH471056 Genomic DNA. Translation: EAX05210.1. CH471056 Genomic DNA. Translation: EAX05211.1. BC063856 mRNA. Translation: AAH63856.1. AY590694 mRNA. Translation: AAT06102.1. AF041037 mRNA. Translation: AAC39566.1.
IPI	IPI00306505.
RefSeq	NP_005832.1. NM_005841.1. NP_955359.1. NM_199327.1.
UniGene	Hs.436944.
3D structure databases
ProteinModelPortal	O43609.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43609. 6 interactions.
MINT	MINT-6169704.
STRING	O43609.
PTM databases
PhosphoSite	O43609.
Proteomic databases
PRIDE	O43609.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000339241; ENSP00000343785; ENSG00000164056. ENST00000394339; ENSP00000377871; ENSG00000164056.
GeneID	10252.
KEGG	hsa:10252.
UCSC	uc003ifa.1. human.
Organism-specific databases
CTD	10252.
GeneCards	GC04P124317.
H-InvDB	HIX0004486.
HGNC	HGNC:11269. SPRY1.
HPA	CAB013538.
MIM	602465. gene.
neXtProt	NX_O43609.
PharmGKB	PA36098.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12819.
GeneTree	ENSGT00390000003535.
HOGENOM	HBG444143.
HOVERGEN	HBG003544.
InParanoid	O43609.
OMA	NTVYCKL.
PhylomeDB	O43609.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	O43609.
Bgee	O43609.
CleanEx	HS_SPRY1.
Genevestigator	O43609.
GermOnline	ENSG00000164056. Homo sapiens.
Family and domain databases
InterPro	IPR007875. Sprouty. [Graphical view]
KO	K04704.
Pfam	PF05210. Sprouty. 1 hit. [Graphical view]
PROSITE	PS51227. SPR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	38846.
SOURCE	Search...

Entry information

Entry name

SPY1_HUMAN

Accession

Primary (citable) accession number: O43609
Secondary accession number(s): D3DNX6, Q6PNE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 10, 2004
Last modified:	January 25, 2012
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.