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Protein

Exportin-T

Gene

XPOT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the nuclear export of aminoacylated tRNAs. In the nucleus binds to tRNA and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the tRNA from the export receptor. XPOT then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-33500-MONOMER.
ReactomeiR-HSA-6784531. tRNA processing in the nucleus.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Exportin-T
Alternative name(s):
Exportin(tRNA)
tRNA exportin
Gene namesi
Name:XPOT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:12826. XPOT.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Nuclear, once bound to tRNA and Ran the complex translocates to the cytoplasm. Shuttles between the nucleus and the cytoplasm.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • nuclear matrix Source: GO_Central
  • nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi405 – 409RKQLK → AAQLA: Abolishes binding to tRNA. Does not abolish shuttling behavior. 1 Publication5
Mutagenesisi539 – 543KVRSR → AVRSA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication5
Mutagenesisi547 – 551LFSRF → AFSRA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication5
Mutagenesisi548 – 552FSRFV → ASRFA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication5
Mutagenesisi550 – 557RFVKSLNK → AFVASLNA: Abolishes binding to tRNA. Does not abolish shuttling behavior. 1 Publication8

Organism-specific databases

DisGeNETi11260.
OpenTargetsiENSG00000184575.
PharmGKBiPA37419.

Polymorphism and mutation databases

BioMutaiXPOT.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002047161 – 962Exportin-TAdd BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei634N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO43592.
MaxQBiO43592.
PaxDbiO43592.
PeptideAtlasiO43592.
PRIDEiO43592.

PTM databases

iPTMnetiO43592.
PhosphoSitePlusiO43592.

Expressioni

Gene expression databases

BgeeiENSG00000184575.
CleanExiHS_XPOT.
ExpressionAtlasiO43592. baseline and differential.
GenevisibleiO43592. HS.

Organism-specific databases

HPAiHPA048067.
HPA057602.

Interactioni

Subunit structurei

Found in a complex with XPOT, Ran and tRNA. Probably found in a complex with nucleoporins. Interacts with Ran and tRNA in a GTP-dependent manner.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116420. 42 interactors.
IntActiO43592. 23 interactors.
MINTiMINT-1138512.
STRINGi9606.ENSP00000327821.

Structurei

3D structure databases

ProteinModelPortaliO43592.
SMRiO43592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 385Necessary for interaction with Ran, nuclear localization and nuclear importAdd BLAST385
Regioni443 – 962Necessary for tRNA-binding, cytoplasmic localization and nuclear exportAdd BLAST520

Sequence similaritiesi

Belongs to the exportin family.Curated

Phylogenomic databases

eggNOGiKOG2021. Eukaryota.
ENOG410XZDZ. LUCA.
GeneTreeiENSGT00390000007890.
HOGENOMiHOG000007104.
HOVERGENiHBG094163.
InParanoidiO43592.
KOiK14288.
OMAiMRYDETS.
OrthoDBiEOG091G04A1.
PhylomeDBiO43592.
TreeFamiTF314001.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013598. Exportin-1/Importin-b-like.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
PF08389. Xpo1. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

O43592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEQALLGLN PNADSDFRQR ALAYFEQLKI SPDAWQVCAE ALAQRTYSDD
60 70 80 90 100
HVKFFCFQVL EHQVKYKYSE LTTVQQQLIR ETLISWLQAQ MLNPQPEKTF
110 120 130 140 150
IRNKAAQVFA LLFVTEYLTK WPKFFFDILS VVDLNPRGVD LYLRILMAID
160 170 180 190 200
SELVDRDVVH TSEEARRNTL IKDTMREQCI PNLVESWYQI LQNYQFTNSE
210 220 230 240 250
VTCQCLEVVG AYVSWIDLSL IANDRFINML LGHMSIEVLR EEACDCLFEV
260 270 280 290 300
VNKGMDPVDK MKLVESLCQV LQSAGFFSID QEEDVDFLAR FSKLVNGMGQ
310 320 330 340 350
SLIVSWSKLI KNGDIKNAQE ALQAIETKVA LMLQLLIHED DDISSNIIGF
360 370 380 390 400
CYDYLHILKQ LTVLSDQQKA NVEAIMLAVM KKLTYDEEYN FENEGEDEAM
410 420 430 440 450
FVEYRKQLKL LLDRLAQVSP ELLLASVRRV FSSTLQNWQT TRFMEVEVAI
460 470 480 490 500
RLLYMLAEAL PVSHGAHFSG DVSKASALQD MMRTLVTSGV SSYQHTSVTL
510 520 530 540 550
EFFETVVRYE KFFTVEPQHI PCVLMAFLDH RGLRHSSAKV RSRTAYLFSR
560 570 580 590 600
FVKSLNKQMN PFIEDILNRI QDLLELSPPE NGHQSLLSSD DQLFIYETAG
610 620 630 640 650
VLIVNSEYPA ERKQALMRNL LTPLMEKFKI LLEKLMLAQD EERQASLADC
660 670 680 690 700
LNHAVGFASR TSKAFSNKQT VKQCGCSEVY LDCLQTFLPA LSCPLQKDIL
710 720 730 740 750
RSGVRTFLHR MIICLEEEVL PFIPSASEHM LKDCEAKDLQ EFIPLINQIT
760 770 780 790 800
AKFKIQVSPF LQQMFMPLLH AIFEVLLRPA EENDQSAALE KQMLRRSYFA
810 820 830 840 850
FLQTVTGSGM SEVIANQGAE NVERVLVTVI QGAVEYPDPI AQKTCFIILS
860 870 880 890 900
KLVELWGGKD GPVGFADFVY KHIVPACFLA PLKQTFDLAD AQTVLALSEC
910 920 930 940 950
AVTLKTIHLK RGPECVQYLQ QEYLPSLQVA PEIIQEFCQA LQQPDAKVFK
960
NYLKVFFQRA KP
Length:962
Mass (Da):109,964
Last modified:May 30, 2006 - v2
Checksum:i2F407EEDEC7C2996
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti360Q → R in AAC39793 (PubMed:9660920).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_026528526A → V.1 PublicationCorresponds to variant rs17851795dbSNPEnsembl.1
Natural variantiVAR_048962716E → D.Corresponds to variant rs1051396dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039022 mRNA. Translation: AAC39793.1.
Y16414 mRNA. Translation: CAA76202.1.
AC135279 Genomic DNA. No translation available.
BC000950 mRNA. Translation: AAH00950.1.
BC020569 mRNA. Translation: AAH20569.1.
CCDSiCCDS31852.1.
RefSeqiNP_009166.2. NM_007235.4.
XP_016874237.1. XM_017018748.1.
UniGeneiHs.592579.
Hs.85951.

Genome annotation databases

EnsembliENST00000332707; ENSP00000327821; ENSG00000184575.
GeneIDi11260.
KEGGihsa:11260.
UCSCiuc001ssb.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039022 mRNA. Translation: AAC39793.1.
Y16414 mRNA. Translation: CAA76202.1.
AC135279 Genomic DNA. No translation available.
BC000950 mRNA. Translation: AAH00950.1.
BC020569 mRNA. Translation: AAH20569.1.
CCDSiCCDS31852.1.
RefSeqiNP_009166.2. NM_007235.4.
XP_016874237.1. XM_017018748.1.
UniGeneiHs.592579.
Hs.85951.

3D structure databases

ProteinModelPortaliO43592.
SMRiO43592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116420. 42 interactors.
IntActiO43592. 23 interactors.
MINTiMINT-1138512.
STRINGi9606.ENSP00000327821.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiO43592.
PhosphoSitePlusiO43592.

Polymorphism and mutation databases

BioMutaiXPOT.

Proteomic databases

EPDiO43592.
MaxQBiO43592.
PaxDbiO43592.
PeptideAtlasiO43592.
PRIDEiO43592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000332707; ENSP00000327821; ENSG00000184575.
GeneIDi11260.
KEGGihsa:11260.
UCSCiuc001ssb.4. human.

Organism-specific databases

CTDi11260.
DisGeNETi11260.
GeneCardsiXPOT.
HGNCiHGNC:12826. XPOT.
HPAiHPA048067.
HPA057602.
MIMi603180. gene.
neXtProtiNX_O43592.
OpenTargetsiENSG00000184575.
PharmGKBiPA37419.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2021. Eukaryota.
ENOG410XZDZ. LUCA.
GeneTreeiENSGT00390000007890.
HOGENOMiHOG000007104.
HOVERGENiHBG094163.
InParanoidiO43592.
KOiK14288.
OMAiMRYDETS.
OrthoDBiEOG091G04A1.
PhylomeDBiO43592.
TreeFamiTF314001.

Enzyme and pathway databases

BioCyciZFISH:G66-33500-MONOMER.
ReactomeiR-HSA-6784531. tRNA processing in the nucleus.

Miscellaneous databases

ChiTaRSiXPOT. human.
GeneWikiiXPOT.
GenomeRNAii11260.
PROiO43592.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184575.
CleanExiHS_XPOT.
ExpressionAtlasiO43592. baseline and differential.
GenevisibleiO43592. HS.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013598. Exportin-1/Importin-b-like.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
PF08389. Xpo1. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiXPOT_HUMAN
AccessioniPrimary (citable) accession number: O43592
Secondary accession number(s): A6NLH1
, O43784, Q8WUG2, Q9BVS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: May 30, 2006
Last modified: November 30, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.