O43581 (SYT7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptotagmin-7

Alternative name(s):
IPCA-7
Prostate cancer-associated protein 7
Synaptotagmin VII
Short name=SytVII

Gene names

Name:	SYT7
Synonyms:	PCANAP7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis By similarity.
Cofactor	Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.
Subunit structure	Interacts with SYNCRIP isoform 3 C-terminus By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein By similarity.
Tissue specificity	Expressed in a variety of adult and fetal tissues.
Domain	The second C2 domain/C2B is responsible for SYNCRIP binding By similarity.
Sequence similarities	Belongs to the synaptotagmin family. Contains 2 C2 domains.
Sequence caution	The sequence AAB92667.1 differs from that shown. Reason: Frameshift at positions 6, 10 and 15.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	plasma membrane repair Inferred from electronic annotation. Source: Compara regulation of calcium ion-dependent exocytosis Inferred from electronic annotation. Source: Compara regulation of insulin secretion Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosome Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: Compara transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43581-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43581-2)

The sequence of this isoform differs from the canonical sequence as follows:
     71-71: I → INGTLLSGAK...HLAAGKLNLS

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 403

403

Synaptotagmin-7

PRO_0000183957

Regions

Topological domain

1 – 16

16

Vesicular Potential

Transmembrane

17 – 37

21

Helical; Potential

Topological domain

38 – 403

366

Cytoplasmic Potential

Domain

137 – 239

103

C2 1

Domain

268 – 371

104

C2 2

Sites

Metal binding

166

1

Calcium 1 By similarity

Metal binding

166

1

Calcium 2 By similarity

Metal binding

172

1

Calcium 1 By similarity

Metal binding

225

1

Calcium 1 By similarity

Metal binding

225

1

Calcium 2 By similarity

Metal binding

227

1

Calcium 1 By similarity

Metal binding

227

1

Calcium 2 By similarity

Metal binding

227

1

Calcium 3 By similarity

Metal binding

230

1

Calcium 3 By similarity

Metal binding

233

1

Calcium 2 By similarity

Metal binding

233

1

Calcium 3 By similarity

Natural variations

Alternative sequence

71

1

I → INGTLLSGAKVAAAAGLAVE REGRLGEKPAPVPPPGEDAL RSGGAAPSEPGSGGKAGRGR WRTVQSHLAAGKLNLS in isoform 2.

VSP_045991

Natural variant

332

1

I → N. Ref.1
Corresponds to variant rs407740 [ dbSNP | Ensembl ].

VAR_052241

Experimental info

Sequence conflict

154

1

V → L in AAB92667. Ref.1

Sequence conflict

188

1

E → K in AAB92667. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

403

Helix Strand Turn

Details...

Beta strand

138 – 145

8

Beta strand

147 – 149

3

Beta strand

152 – 161

10

Beta strand

166 – 169

4

Beta strand

173 – 181

9

Beta strand

184 – 188

5

Beta strand

202 – 206

5

Helix

211 – 214

4

Beta strand

217 – 225

9

Beta strand

228 – 231

4

Beta strand

233 – 241

9

Turn

242 – 244

3

Beta strand

251 – 255

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 23, 2008. Version 3. Checksum: C9BFB26D298EDBE4	FASTA	403	45,501
	10 20 30 40 50 60 MYRDPEAASP GAPSRDVLLV SAIITVSLSV TVVLCGLCHW CQRKLGKRYK NSLETVGTPD 70 80 90 100 110 120 SGRGRSEKKA IKLPAGGKAV NTAPVPGQTP HDESDRRTEP RSSVSDLVNS LTSEMLMLSP 130 140 150 160 170 180 GSEEDEAHEG CSRENLGRIQ FSVGYNFQES TLTVKIMKAQ ELPAKDFSGT SDPFVKIYLL 190 200 210 220 230 240 PDKKHKLETK VKRKNLNPHW NETFLFEGFP YEKVVQRILY LQVLDYDRFS RNDPIGEVSI 250 260 270 280 290 300 PLNKVDLTQM QTFWKDLKPC SDGSGSRGEL LLSLCYNPSA NSIIVNIIKA RNLKAMDIGG 310 320 330 340 350 360 TSDPYVKVWL MYKDKRVEKK KTVTMKRNLN PIFNESFAFD IPTEKLRETT IIITVMDKDK 370 380 390 400 LSRNDVIGKI YLSWKSGPGE VKHWKDMIAR PRQPVAQWHQ LKA
	Isoform 2 [UniParc]. Checksum: 6F6B1DD2C9AFF15F	FASTA	478	52,821
	10 20 30 40 50 60 MYRDPEAASP GAPSRDVLLV SAIITVSLSV TVVLCGLCHW CQRKLGKRYK NSLETVGTPD 70 80 90 100 110 120 SGRGRSEKKA INGTLLSGAK VAAAAGLAVE REGRLGEKPA PVPPPGEDAL RSGGAAPSEP 130 140 150 160 170 180 GSGGKAGRGR WRTVQSHLAA GKLNLSKLPA GGKAVNTAPV PGQTPHDESD RRTEPRSSVS 190 200 210 220 230 240 DLVNSLTSEM LMLSPGSEED EAHEGCSREN LGRIQFSVGY NFQESTLTVK IMKAQELPAK 250 260 270 280 290 300 DFSGTSDPFV KIYLLPDKKH KLETKVKRKN LNPHWNETFL FEGFPYEKVV QRILYLQVLD 310 320 330 340 350 360 YDRFSRNDPI GEVSIPLNKV DLTQMQTFWK DLKPCSDGSG SRGELLLSLC YNPSANSIIV 370 380 390 400 410 420 NIIKARNLKA MDIGGTSDPY VKVWLMYKDK RVEKKKTVTM KRNLNPIFNE SFAFDIPTEK 430 440 450 460 470 LRETTIIITV MDKDKLSRND VIGKIYLSWK SGPGEVKHWK DMIARPRQPV AQWHQLKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transcript mapping of the human chromosome 11q12-q13.1 gene-rich region identifies several newly described conserved genes." Cooper P.R., Nowak N.J., Higgins M.J., Church D.M., Shows T.B. Genomics 49:419-429(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ASN-332.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[5]	"Solution structure of the first C2 domain of synaptotagmin VII." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 132-259.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF038535 mRNA. Translation: AAB92667.1. Frameshift.
AP002754 Genomic DNA. No translation available.
AP003108 Genomic DNA. No translation available.
AP003559 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73956.1.
BC125170 mRNA. Translation: AAI25171.1.
BC125171 mRNA. No translation available.

IPI

IPI00012902.
IPI01014982.

RefSeq

NP_001238994.1. NM_001252065.1.
NP_004191.2. NM_004200.3.

UniGene

Hs.131188.
Hs.684589.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D8K	NMR	-	A	132-259	[»]

ProteinModelPortal

O43581.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000263846.

PTM databases

PhosphoSite

O43581.

Proteomic databases

PaxDb

O43581.

PRIDE

O43581.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263846; ENSP00000263846; ENSG00000011347.
ENST00000540677; ENSP00000444201; ENSG00000011347.

GeneID

9066.

KEGG

hsa:9066.

UCSC

uc001nrv.3. human.

Organism-specific databases

CTD

9066.

GeneCards

GC11M061282.

H-InvDB

HIX0009693.

HGNC

HGNC:11514. SYT7.

HPA

HPA012869.

MIM

604146. gene.

neXtProt

NX_O43581.

PharmGKB

PA36295.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5038.

HOGENOM

HOG000232126.

HOVERGEN

HBG005010.

InParanoid

O43581.

OrthoDB

EOG48KRB3.

PhylomeDB

O43581.

Gene expression databases

ArrayExpress

O43581.

Bgee

O43581.

CleanEx

HS_SYT7.

Genevestigator

O43581.

GermOnline

ENSG00000011347. Homo sapiens.

Family and domain databases

InterPro

IPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR020477. C2_dom.
IPR018029. C2_membr_targeting.
IPR001565. Synaptotagmin.
IPR015427. Synaptotagmin7.
[Graphical view]

PANTHER

PTHR10024:SF29. PTHR10024:SF29. 1 hit.

Pfam

PF00168. C2. 2 hits.
[Graphical view]

PRINTS

PR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.

SMART

SM00239. C2. 2 hits.
[Graphical view]

SUPFAM

SSF49562. C2_CaLB. 2 hits.

PROSITE

PS50004. C2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

SYT7. human.

EvolutionaryTrace

O43581.

GenomeRNAi

9066.

NextBio

33971.

SOURCE

Search...

Entry information

Entry name

SYT7_HUMAN

Accession

Primary (citable) accession number: O43581
Secondary accession number(s): F5GZU9, Q08AH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.