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Protein

A-kinase anchor protein 10, mitochondrial

Gene

AKAP10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase (By similarity).By similarity

GO - Biological processi

  • blood coagulation Source: Reactome
  • protein localization Source: ProtInc
  • signal transduction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 10, mitochondrial
Short name:
AKAP-10
Alternative name(s):
Dual specificity A kinase-anchoring protein 2
Short name:
D-AKAP-2
Protein kinase A-anchoring protein 10
Short name:
PRKA10
Gene namesi
Name:AKAP10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:368. AKAP10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion

Pathology & Biotechi

Involvement in diseasei

Sudden cardiac death (SCD)1 Publication
Disease susceptibility is associated with variations affecting the gene represented in this entry. Increased susceptibility to sudden cardiac death may be conferred by AKAP10 variants that are associated with markers of low vagus nerve sensitivity, e.g. fast basal heart rate and low heart rate variability.
Disease descriptionUnexpected rapid death due to cardiovascular collapse in a short time period, generally within one hour of initial symptoms. It is usually caused by the worsening of existing heart diseases. The sudden onset of symptoms, such as chest pain and cardiac arrhythmias, particularly ventricular tachycardia, can lead to the loss of consciousness and cardiac arrest followed by biological death.
See also OMIM:115080

Organism-specific databases

MalaCardsiAKAP10.
MIMi115080. phenotype.
PharmGKBiPA24662.

Polymorphism and mutation databases

BioMutaiAKAP10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828MitochondrionSequence analysisAdd
BLAST
Chaini29 – 662634A-kinase anchor protein 10, mitochondrialPRO_0000030404Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521PhosphoserineCombined sources
Modified residuei189 – 1891PhosphoserineCombined sources
Modified residuei281 – 2811PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO43572.
MaxQBiO43572.
PaxDbiO43572.
PeptideAtlasiO43572.
PRIDEiO43572.

PTM databases

iPTMnetiO43572.
PhosphoSiteiO43572.
SwissPalmiO43572.

Expressioni

Gene expression databases

BgeeiO43572.
CleanExiHS_AKAP10.
ExpressionAtlasiO43572. baseline and differential.
GenevisibleiO43572. HS.

Organism-specific databases

HPAiHPA028901.

Interactioni

Protein-protein interaction databases

BioGridi116385. 6 interactions.
DIPiDIP-48753N.
IntActiO43572. 1 interaction.
MINTiMINT-1473023.
STRINGi9606.ENSP00000225737.

Structurei

Secondary structure

1
662
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi629 – 65325Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IM4X-ray2.28C623-662[»]
3TMHX-ray3.80D/H/L623-662[»]
ProteinModelPortaliO43572.
SMRiO43572. Positions 296-367, 371-504, 635-662.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43572.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 369245RGS 1PROSITE-ProRule annotationAdd
BLAST
Domaini379 – 505127RGS 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni634 – 64714PKA-RII subunit bindingAdd
BLAST

Domaini

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Sequence similaritiesi

Contains 2 RGS domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3590. Eukaryota.
ENOG410YH1M. LUCA.
GeneTreeiENSGT00390000015077.
HOGENOMiHOG000273900.
HOVERGENiHBG050469.
InParanoidiO43572.
KOiK16526.
OMAiFSAMEQE.
OrthoDBiEOG7B8S3S.
PhylomeDBiO43572.
TreeFamiTF105409.

Family and domain databases

InterProiIPR016137. RGS.
[Graphical view]
PfamiPF00615. RGS. 2 hits.
[Graphical view]
SMARTiSM00315. RGS. 2 hits.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 3 hits.
PROSITEiPS50132. RGS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGAGPSPRQ SPRTLRPDPG PAMSFFRRKV KGKEQEKTSD VKSIKASISV
60 70 80 90 100
HSPQKSTKNH ALLEAAGPSH VAINAISANM DSFSSSRTAT LKKQPSHMEA
110 120 130 140 150
AHFGDLGRSC LDYQTQETKS SLSKTLEQVL HDTIVLPYFI QFMELRRMEH
160 170 180 190 200
LVKFWLEAES FHSTTWSRIR AHSLNTVKQS SLAEPVSPSK KHETTASFLT
210 220 230 240 250
DSLDKRLEDS GSAQLFMTHS EGIDLNNRTN STQNHLLLSQ ECDSAHSLRL
260 270 280 290 300
EMARAGTHQV SMETQESSST LTVASRNSPA SPLKELSGKL MKSIEQDAVN
310 320 330 340 350
TFTKYISPDA AKPIPITEAM RNDIIARICG EDGQVDPNCF VLAQSIVFSA
360 370 380 390 400
MEQEHFSEFL RSHHFCKYQI EVLTSGTVYL ADILFCESAL FYFSEYMEKE
410 420 430 440 450
DAVNILQFWL AADNFQSQLA AKKGQYDGQE AQNDAMILYD KYFSLQATHP
460 470 480 490 500
LGFDDVVRLE IESNICREGG PLPNCFTTPL RQAWTTMEKV FLPGFLSSNL
510 520 530 540 550
YYKYLNDLIH SVRGDEFLGG NVSLTAPGSV GPPDESHPGS SDSSASQSSV
560 570 580 590 600
KKASIKILKN FDEAIIVDAA SLDPESLYQR TYAGKMTFGR VSDLGQFIRE
610 620 630 640 650
SEPEPDVRKS KGSMFSQAMK KWVQGNTDEA QEELAWKIAK MIVSDIMQQA
660
QYDQPLEKST KL
Length:662
Mass (Da):73,818
Last modified:July 19, 2005 - v2
Checksum:iB7A6D92CA2D3D794
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771V → M in AAB92260 (Ref. 2) Curated
Sequence conflicti524 – 5241L → P in AAB92260 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti249 – 2491R → H.
Corresponds to variant rs2108978 [ dbSNP | Ensembl ].
VAR_024607
Natural varianti646 – 6461I → V Associated with increased basal heart rate and decreased heart rate variability. 1 Publication
Corresponds to variant rs203462 [ dbSNP | Ensembl ].
VAR_024608

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037439 mRNA. Translation: AAB92260.1.
AK312438 mRNA. Translation: BAG35347.1.
CH471212 Genomic DNA. Translation: EAW50913.1.
BC017055 mRNA. Translation: AAH17055.1.
CCDSiCCDS11214.1.
RefSeqiNP_009133.2. NM_007202.3.
UniGeneiHs.642676.

Genome annotation databases

EnsembliENST00000225737; ENSP00000225737; ENSG00000108599.
GeneIDi11216.
KEGGihsa:11216.
UCSCiuc002gwo.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037439 mRNA. Translation: AAB92260.1.
AK312438 mRNA. Translation: BAG35347.1.
CH471212 Genomic DNA. Translation: EAW50913.1.
BC017055 mRNA. Translation: AAH17055.1.
CCDSiCCDS11214.1.
RefSeqiNP_009133.2. NM_007202.3.
UniGeneiHs.642676.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IM4X-ray2.28C623-662[»]
3TMHX-ray3.80D/H/L623-662[»]
ProteinModelPortaliO43572.
SMRiO43572. Positions 296-367, 371-504, 635-662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116385. 6 interactions.
DIPiDIP-48753N.
IntActiO43572. 1 interaction.
MINTiMINT-1473023.
STRINGi9606.ENSP00000225737.

PTM databases

iPTMnetiO43572.
PhosphoSiteiO43572.
SwissPalmiO43572.

Polymorphism and mutation databases

BioMutaiAKAP10.

Proteomic databases

EPDiO43572.
MaxQBiO43572.
PaxDbiO43572.
PeptideAtlasiO43572.
PRIDEiO43572.

Protocols and materials databases

DNASUi11216.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000225737; ENSP00000225737; ENSG00000108599.
GeneIDi11216.
KEGGihsa:11216.
UCSCiuc002gwo.5. human.

Organism-specific databases

CTDi11216.
GeneCardsiAKAP10.
HGNCiHGNC:368. AKAP10.
HPAiHPA028901.
MalaCardsiAKAP10.
MIMi115080. phenotype.
604694. gene.
neXtProtiNX_O43572.
PharmGKBiPA24662.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3590. Eukaryota.
ENOG410YH1M. LUCA.
GeneTreeiENSGT00390000015077.
HOGENOMiHOG000273900.
HOVERGENiHBG050469.
InParanoidiO43572.
KOiK16526.
OMAiFSAMEQE.
OrthoDBiEOG7B8S3S.
PhylomeDBiO43572.
TreeFamiTF105409.

Enzyme and pathway databases

ReactomeiR-HSA-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

ChiTaRSiAKAP10. human.
EvolutionaryTraceiO43572.
GeneWikiiAKAP10.
GenomeRNAii11216.
PROiO43572.
SOURCEiSearch...

Gene expression databases

BgeeiO43572.
CleanExiHS_AKAP10.
ExpressionAtlasiO43572. baseline and differential.
GenevisibleiO43572. HS.

Family and domain databases

InterProiIPR016137. RGS.
[Graphical view]
PfamiPF00615. RGS. 2 hits.
[Graphical view]
SMARTiSM00315. RGS. 2 hits.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 3 hits.
PROSITEiPS50132. RGS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and mitochondrial localization of full-length D-AKAP2, a protein kinase A anchoring protein."
    Wang L., Sunahara R.K., Krumins A., Perkins G., Crochiere M.L., Mackey M., Bell S., Ellisman M.H., Taylor S.S.
    Proc. Natl. Acad. Sci. U.S.A. 98:3220-3225(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "Homo sapiens protein kinase A anchoring protein with an RGS domain."
    Chatterjee T.K., Fisher R.A.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189 AND SER-281, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.
  9. "Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation."
    Tingley W.G., Pawlikowska L., Zaroff J.G., Kim T., Nguyen T., Young S.G., Vranizan K., Kwok P.Y., Whooley M.A., Conklin B.R.
    Proc. Natl. Acad. Sci. U.S.A. 104:8461-8466(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT VAL-646, INVOLVEMENT IN SUSCEPTIBILITY TO SCD.

Entry informationi

Entry nameiAKA10_HUMAN
AccessioniPrimary (citable) accession number: O43572
Secondary accession number(s): B2R650, Q96AJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 19, 2005
Last modified: July 6, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.