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O43567 (RNF13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase RNF13

EC=6.3.2.-
Alternative name(s):
RING finger protein 13
Gene names
Name:RNF13
Synonyms:RZF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation. Ref.9

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane; Single-pass membrane protein By similarity. Lysosome membrane. Nucleus inner membrane By similarity. Note: Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane By similarity. Ref.8 Ref.9

Tissue specificity

Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level). Ref.9

Domain

The RING-type zinc finger domain is required for E3 ligase activity.

Post-translational modification

Auto-ubiquitinated.

Sequence similarities

Contains 1 PA (protease associated) domain.

Contains 1 RING-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Potential
Chain35 – 381347E3 ubiquitin-protein ligase RNF13
PRO_0000056054

Regions

Topological domain35 – 182148Lumenal Potential
Transmembrane183 – 20321Helical; Potential
Topological domain204 – 381178Cytoplasmic Potential
Domain65 – 16096PA
Zinc finger240 – 28243RING-type; atypical

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Ref.9

Experimental info

Mutagenesis431N → A: No effect on glycosylation. Ref.9
Mutagenesis881N → A: Loss of glycosylation. Ref.9
Mutagenesis2581C → A: Complete loss of E3 ligase activity; when associated with A-260. Ref.9
Mutagenesis2601H → A: Complete loss of E3 ligase activity; when associated with A-258. Ref.9
Mutagenesis2701W → A: Drastically reduces E3 ligase activity. Ref.9

Sequences

Sequence LengthMass (Da)Tools
O43567 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 4600727D0F197653

FASTA38142,814
        10         20         30         40         50         60 
MLLSIGMLML SATQVYTILT VQLFAFLNLL PVEADILAYN FENASQTFDD LPARFGYRLP 

        70         80         90        100        110        120 
AEGLKGFLIN SKPENACEPI VPPPVKDNSS GTFIVLIRRL DCNFDIKVLN AQRAGYKAAI 

       130        140        150        160        170        180 
VHNVDSDDLI SMGSNDIEVL KKIDIPSVFI GESSANSLKD EFTYEKGGHL ILVPEFSLPL 

       190        200        210        220        230        240 
EYYLIPFLII VGICLILIVI FMITKFVQDR HRARRNRLRK DQLKKLPVHK FKKGDEYDVC 

       250        260        270        280        290        300 
AICLDEYEDG DKLRILPCSH AYHCKCVDPW LTKTKKTCPV CKQKVVPSQG DSDSDTDSSQ 

       310        320        330        340        350        360 
EENEVTEHTP LLRPLASVSA QSFGALSESR SHQNMTESSD YEEDDNEDTD SSDAENEINE 

       370        380 
HDVVVQLQPN GERDYNIANT V 

« Hide

References

« Hide 'large scale' references
[1]"The gene for a RING zinc finger protein is expressed in the inner chick ear after noise exposure."
Lomax M.I., Warner S.J., Bersirli C.G., Gong T.-W.L.
Prim. Sens. Neuron 2:305-316(1998)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Yu W., Gibbs R.A.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Glial tumor and Spleen.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Urinary bladder.
[8]"Integral and associated lysosomal membrane proteins."
Schroeder B., Wrocklage C., Pan C., Jaeger R., Koesters B., Schaefer H., Elsaesser H.-P., Mann M., Hasilik A.
Traffic 8:1676-1686(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Placenta.
[9]"RNF13: a novel RING-type ubiquitin ligase over-expressed in pancreatic cancer."
Zhang Q., Meng Y., Zhang L., Chen J., Zhu D.
Cell Res. 19:348-357(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AUTOUBIQUITINATION, GLYCOSYLATION AT ASN-88, MUTAGENESIS OF ASN-43; ASN-88; CYS-258; HIS-260 AND TRP-270.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF037204 mRNA. Translation: AAC03769.1.
AF070558 mRNA. Translation: AAC28641.1.
AK313304 mRNA. Translation: BAG36109.1.
AK090638 mRNA. Translation: BAG52202.1.
CR456804 mRNA. Translation: CAG33085.1.
AC069216 Genomic DNA. No translation available.
AC117395 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78859.1.
CH471052 Genomic DNA. Translation: EAW78861.1.
CH471052 Genomic DNA. Translation: EAW78863.1.
BC009803 mRNA. Translation: AAH09803.1.
BC009781 mRNA. Translation: AAH09781.1.
RefSeqNP_009213.1. NM_007282.4.
NP_899237.1. NM_183381.2.
UniGeneHs.12333.

3D structure databases

ProteinModelPortalO43567.
SMRO43567. Positions 59-162, 240-286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116470. 13 interactions.
IntActO43567. 7 interactions.
MINTMINT-4657952.
STRING9606.ENSP00000341361.

PTM databases

PhosphoSiteO43567.

Proteomic databases

PaxDbO43567.
PRIDEO43567.

Protocols and materials databases

DNASU11342.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000344229; ENSP00000341361; ENSG00000082996.
ENST00000392894; ENSP00000376628; ENSG00000082996.
GeneID11342.
KEGGhsa:11342.
UCSCuc003exn.4. human.

Organism-specific databases

CTD11342.
GeneCardsGC03P149531.
HGNCHGNC:10057. RNF13.
HPAHPA008709.
MIM609247. gene.
neXtProtNX_O43567.
PharmGKBPA34422.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5540.
HOGENOMHOG000234362.
HOVERGENHBG063762.
InParanoidO43567.
KOK15692.
OMAMGSNDID.
OrthoDBEOG7NW68S.
PhylomeDBO43567.
TreeFamTF317486.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressO43567.
BgeeO43567.
CleanExHS_RNF13.
GenevestigatorO43567.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR003137. Protease-assoc_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF02225. PA. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
PROSITEPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRNF13. human.
GeneWikiRNF13.
GenomeRNAi11342.
NextBio43096.
PROO43567.
SOURCESearch...

Entry information

Entry nameRNF13_HUMAN
AccessionPrimary (citable) accession number: O43567
Secondary accession number(s): Q6IBJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM