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Protein

E3 ubiquitin-protein ligase RNF13

Gene

RNF13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri240 – 282RING-type; atypicalPROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000082996-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF13 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 13
Gene namesi
Name:RNF13
Synonyms:RZF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:10057. RNF13.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini35 – 182LumenalSequence analysisAdd BLAST148
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 381CytoplasmicSequence analysisAdd BLAST178

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43N → A: No effect on glycosylation. 1 Publication1
Mutagenesisi88N → A: Loss of glycosylation. 1 Publication1
Mutagenesisi258C → A: Complete loss of E3 ligase activity; when associated with A-260. 1 Publication1
Mutagenesisi260H → A: Complete loss of E3 ligase activity; when associated with A-258. 1 Publication1
Mutagenesisi270W → A: Drastically reduces E3 ligase activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000082996.
PharmGKBiPA34422.

Polymorphism and mutation databases

BioMutaiRNF13.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Sequence analysisAdd BLAST34
ChainiPRO_000005605435 – 381E3 ubiquitin-protein ligase RNF13Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi88N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

Auto-ubiquitinated.1 Publication

Keywords - PTMi

Glycoprotein, Ubl conjugation

Proteomic databases

EPDiO43567.
MaxQBiO43567.
PaxDbiO43567.
PeptideAtlasiO43567.
PRIDEiO43567.

PTM databases

iPTMnetiO43567.
PhosphoSitePlusiO43567.

Expressioni

Tissue specificityi

Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000082996.
CleanExiHS_RNF13.
ExpressionAtlasiO43567. baseline and differential.
GenevisibleiO43567. HS.

Organism-specific databases

HPAiHPA008709.
HPA064784.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
UBE2NP610882EBI-2129183,EBI-1052908

Protein-protein interaction databases

BioGridi116470. 36 interactors.
IntActiO43567. 11 interactors.
MINTiMINT-4657952.
STRINGi9606.ENSP00000341361.

Structurei

3D structure databases

ProteinModelPortaliO43567.
SMRiO43567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 160PAAdd BLAST96

Domaini

The RING-type zinc finger domain is required for E3 ligase activity.

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri240 – 282RING-type; atypicalPROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG4628. Eukaryota.
ENOG410Z5DF. LUCA.
GeneTreeiENSGT00760000119057.
HOGENOMiHOG000234362.
HOVERGENiHBG063762.
InParanoidiO43567.
KOiK15692.
OMAiNMTESSE.
OrthoDBiEOG091G08WC.
PhylomeDBiO43567.
TreeFamiTF317486.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR003137. PA_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43567-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLSIGMLML SATQVYTILT VQLFAFLNLL PVEADILAYN FENASQTFDD
60 70 80 90 100
LPARFGYRLP AEGLKGFLIN SKPENACEPI VPPPVKDNSS GTFIVLIRRL
110 120 130 140 150
DCNFDIKVLN AQRAGYKAAI VHNVDSDDLI SMGSNDIEVL KKIDIPSVFI
160 170 180 190 200
GESSANSLKD EFTYEKGGHL ILVPEFSLPL EYYLIPFLII VGICLILIVI
210 220 230 240 250
FMITKFVQDR HRARRNRLRK DQLKKLPVHK FKKGDEYDVC AICLDEYEDG
260 270 280 290 300
DKLRILPCSH AYHCKCVDPW LTKTKKTCPV CKQKVVPSQG DSDSDTDSSQ
310 320 330 340 350
EENEVTEHTP LLRPLASVSA QSFGALSESR SHQNMTESSD YEEDDNEDTD
360 370 380
SSDAENEINE HDVVVQLQPN GERDYNIANT V
Length:381
Mass (Da):42,814
Last modified:June 1, 1998 - v1
Checksum:i4600727D0F197653
GO
Isoform 2 (identifier: O43567-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MLLSIGMLMLSATQVYTI → MLILMTSLAWDPTTVSTV
     19-137: Missing.

Note: No experimental confirmation available.
Show »
Length:262
Mass (Da):29,713
Checksum:iDAAF0885999293D3
GO

Sequence cautioni

The sequence AAH17878 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0554301 – 18MLLSI…QVYTI → MLILMTSLAWDPTTVSTV in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_05543119 – 137Missing in isoform 2. 1 PublicationAdd BLAST119

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037204 mRNA. Translation: AAC03769.1.
AF070558 mRNA. Translation: AAC28641.1.
AK313304 mRNA. Translation: BAG36109.1.
AK090638 mRNA. Translation: BAG52202.1.
AK090771 mRNA. Translation: BAG52224.1.
CR456804 mRNA. Translation: CAG33085.1.
AC069216 Genomic DNA. No translation available.
AC117395 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78858.1.
CH471052 Genomic DNA. Translation: EAW78859.1.
CH471052 Genomic DNA. Translation: EAW78860.1.
CH471052 Genomic DNA. Translation: EAW78861.1.
CH471052 Genomic DNA. Translation: EAW78862.1.
CH471052 Genomic DNA. Translation: EAW78863.1.
BC009803 mRNA. Translation: AAH09803.1.
BC009781 mRNA. Translation: AAH09781.1.
BC017878 mRNA. Translation: AAH17878.1. Sequence problems.
CCDSiCCDS3146.1. [O43567-1]
RefSeqiNP_009213.1. NM_007282.4. [O43567-1]
NP_899237.1. NM_183381.2. [O43567-1]
XP_005247149.1. XM_005247092.3. [O43567-2]
XP_011510675.1. XM_011512373.2. [O43567-1]
XP_011510676.1. XM_011512374.2. [O43567-1]
XP_011510678.1. XM_011512376.2. [O43567-2]
XP_016861143.1. XM_017005654.1. [O43567-1]
XP_016861144.1. XM_017005655.1. [O43567-1]
XP_016861145.1. XM_017005656.1. [O43567-2]
XP_016861146.1. XM_017005657.1. [O43567-2]
XP_016861147.1. XM_017005658.1. [O43567-2]
XP_016861148.1. XM_017005659.1. [O43567-2]
UniGeneiHs.12333.

Genome annotation databases

EnsembliENST00000344229; ENSP00000341361; ENSG00000082996. [O43567-1]
ENST00000361785; ENSP00000355268; ENSG00000082996. [O43567-2]
ENST00000392894; ENSP00000376628; ENSG00000082996. [O43567-1]
GeneIDi11342.
KEGGihsa:11342.
UCSCiuc003exn.5. human. [O43567-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037204 mRNA. Translation: AAC03769.1.
AF070558 mRNA. Translation: AAC28641.1.
AK313304 mRNA. Translation: BAG36109.1.
AK090638 mRNA. Translation: BAG52202.1.
AK090771 mRNA. Translation: BAG52224.1.
CR456804 mRNA. Translation: CAG33085.1.
AC069216 Genomic DNA. No translation available.
AC117395 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78858.1.
CH471052 Genomic DNA. Translation: EAW78859.1.
CH471052 Genomic DNA. Translation: EAW78860.1.
CH471052 Genomic DNA. Translation: EAW78861.1.
CH471052 Genomic DNA. Translation: EAW78862.1.
CH471052 Genomic DNA. Translation: EAW78863.1.
BC009803 mRNA. Translation: AAH09803.1.
BC009781 mRNA. Translation: AAH09781.1.
BC017878 mRNA. Translation: AAH17878.1. Sequence problems.
CCDSiCCDS3146.1. [O43567-1]
RefSeqiNP_009213.1. NM_007282.4. [O43567-1]
NP_899237.1. NM_183381.2. [O43567-1]
XP_005247149.1. XM_005247092.3. [O43567-2]
XP_011510675.1. XM_011512373.2. [O43567-1]
XP_011510676.1. XM_011512374.2. [O43567-1]
XP_011510678.1. XM_011512376.2. [O43567-2]
XP_016861143.1. XM_017005654.1. [O43567-1]
XP_016861144.1. XM_017005655.1. [O43567-1]
XP_016861145.1. XM_017005656.1. [O43567-2]
XP_016861146.1. XM_017005657.1. [O43567-2]
XP_016861147.1. XM_017005658.1. [O43567-2]
XP_016861148.1. XM_017005659.1. [O43567-2]
UniGeneiHs.12333.

3D structure databases

ProteinModelPortaliO43567.
SMRiO43567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116470. 36 interactors.
IntActiO43567. 11 interactors.
MINTiMINT-4657952.
STRINGi9606.ENSP00000341361.

PTM databases

iPTMnetiO43567.
PhosphoSitePlusiO43567.

Polymorphism and mutation databases

BioMutaiRNF13.

Proteomic databases

EPDiO43567.
MaxQBiO43567.
PaxDbiO43567.
PeptideAtlasiO43567.
PRIDEiO43567.

Protocols and materials databases

DNASUi11342.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344229; ENSP00000341361; ENSG00000082996. [O43567-1]
ENST00000361785; ENSP00000355268; ENSG00000082996. [O43567-2]
ENST00000392894; ENSP00000376628; ENSG00000082996. [O43567-1]
GeneIDi11342.
KEGGihsa:11342.
UCSCiuc003exn.5. human. [O43567-1]

Organism-specific databases

CTDi11342.
GeneCardsiRNF13.
HGNCiHGNC:10057. RNF13.
HPAiHPA008709.
HPA064784.
MIMi609247. gene.
neXtProtiNX_O43567.
OpenTargetsiENSG00000082996.
PharmGKBiPA34422.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4628. Eukaryota.
ENOG410Z5DF. LUCA.
GeneTreeiENSGT00760000119057.
HOGENOMiHOG000234362.
HOVERGENiHBG063762.
InParanoidiO43567.
KOiK15692.
OMAiNMTESSE.
OrthoDBiEOG091G08WC.
PhylomeDBiO43567.
TreeFamiTF317486.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000082996-MONOMER.

Miscellaneous databases

ChiTaRSiRNF13. human.
GeneWikiiRNF13.
GenomeRNAii11342.
PROiO43567.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000082996.
CleanExiHS_RNF13.
ExpressionAtlasiO43567. baseline and differential.
GenevisibleiO43567. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR003137. PA_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNF13_HUMAN
AccessioniPrimary (citable) accession number: O43567
Secondary accession number(s): A6NC87
, B3KR12, Q05D66, Q6IBJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.