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Protein

DNA repair protein XRCC2

Gene

XRCC2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.4 Publications

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. DNA binding Source: UniProtKB-KW
  3. DNA-dependent ATPase activity Source: InterPro

GO - Biological processi

  1. centrosome organization Source: UniProtKB
  2. DNA repair Source: BHF-UCL
  3. double-strand break repair via homologous recombination Source: UniProtKB
  4. in utero embryonic development Source: Ensembl
  5. meiotic nuclear division Source: ProtInc
  6. mitotic cell cycle Source: UniProtKB
  7. multicellular organism growth Source: Ensembl
  8. negative regulation of neuron apoptotic process Source: Ensembl
  9. positive regulation of neurogenesis Source: Ensembl
  10. regulation of fibroblast apoptotic process Source: Ensembl
  11. response to gamma radiation Source: Ensembl
  12. response to X-ray Source: Ensembl
  13. somitogenesis Source: Ensembl
  14. strand invasion Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein XRCC2
Alternative name(s):
X-ray repair cross-complementing protein 2
Gene namesi
Name:XRCC2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:12829. XRCC2.

Subcellular locationi

Nucleus 1 Publication. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. Rad51B-Rad51C-Rad51D-XRCC2 complex Source: UniProtKB
  4. replication fork Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

Orphaneti227535. Hereditary breast cancer.
PharmGKBiPA37421.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280DNA repair protein XRCC2PRO_0000122948Add
BLAST

Proteomic databases

MaxQBiO43543.
PaxDbiO43543.
PRIDEiO43543.

PTM databases

PhosphoSiteiO43543.

Expressioni

Gene expression databases

BgeeiO43543.
CleanExiHS_XRCC2.
GenevestigatoriO43543.

Interactioni

Subunit structurei

Interacts with RAD51D. Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. In the absence of DNA, the BCDX2 subcomplex XRCC2:RAD51D formed a multimeric ring structure; in the presence of single-stranded DNA it formed a filamentous structure with the ssDNA.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD51CO435022EBI-3918457,EBI-2267048
RAD51DO7577116EBI-3918457,EBI-1055693

Protein-protein interaction databases

BioGridi113350. 10 interactions.
DIPiDIP-24242N.
IntActiO43543. 7 interactions.
MINTiMINT-137755.
STRINGi9606.ENSP00000352271.

Structurei

3D structure databases

ProteinModelPortaliO43543.
SMRiO43543. Positions 41-196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

eggNOGiNOG279678.
GeneTreeiENSGT00390000020445.
HOGENOMiHOG000004802.
HOVERGENiHBG079347.
InParanoidiO43543.
KOiK10879.
OMAiDTDYHFD.
OrthoDBiEOG7K9K4C.
PhylomeDBiO43543.
TreeFamiTF101202.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013632. DNA_recomb/repair_Rad51_C.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSAFHRAES GTELLARLEG RSSLKEIEPN LFADEDSPVH GDILEFHGPE
60 70 80 90 100
GTGKTEMLYH LTARCILPKS EGGLEVEVLF IDTDYHFDML RLVTILEHRL
110 120 130 140 150
SQSSEEIIKY CLGRFFLVYC SSSTHLLLTL YSLESMFCSH PSLCLLILDS
160 170 180 190 200
LSAFYWIDRV NGGESVNLQE STLRKCSQCL EKLVNDYRLV LFATTQTIMQ
210 220 230 240 250
KASSSSEEPS HASRRLCDVD IDYRPYLCKA WQQLVKHRMF FSKQDDSQSS
260 270 280
NQFSLVSRCL KSNSLKKHFF IIGESGVEFC
Length:280
Mass (Da):31,956
Last modified:June 1, 1998 - v1
Checksum:i5656277E74C06074
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti16 – 161A → S.
Corresponds to variant rs4987090 [ dbSNP | Ensembl ].
VAR_020403
Natural varianti188 – 1881R → H.1 Publication
Corresponds to variant rs3218536 [ dbSNP | Ensembl ].
VAR_020404
Natural varianti221 – 2211I → T.1 Publication
Corresponds to variant rs3218537 [ dbSNP | Ensembl ].
VAR_029294

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035587 mRNA. Translation: AAC05369.1.
AC003109 Genomic DNA. Translation: AAC05802.1.
Y08837 mRNA. Translation: CAA70065.1.
Y17033 Genomic DNA. Translation: CAA76597.1.
AF520762 Genomic DNA. Translation: AAM55241.1.
AK313607 mRNA. Translation: BAG36372.1.
CH471173 Genomic DNA. Translation: EAW53968.1.
BC042137 mRNA. Translation: AAH42137.1.
CCDSiCCDS5933.1.
RefSeqiNP_005422.1. NM_005431.1.
UniGeneiHs.647093.

Genome annotation databases

EnsembliENST00000359321; ENSP00000352271; ENSG00000196584.
GeneIDi7516.
KEGGihsa:7516.
UCSCiuc003wld.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035587 mRNA. Translation: AAC05369.1.
AC003109 Genomic DNA. Translation: AAC05802.1.
Y08837 mRNA. Translation: CAA70065.1.
Y17033 Genomic DNA. Translation: CAA76597.1.
AF520762 Genomic DNA. Translation: AAM55241.1.
AK313607 mRNA. Translation: BAG36372.1.
CH471173 Genomic DNA. Translation: EAW53968.1.
BC042137 mRNA. Translation: AAH42137.1.
CCDSiCCDS5933.1.
RefSeqiNP_005422.1. NM_005431.1.
UniGeneiHs.647093.

3D structure databases

ProteinModelPortaliO43543.
SMRiO43543. Positions 41-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113350. 10 interactions.
DIPiDIP-24242N.
IntActiO43543. 7 interactions.
MINTiMINT-137755.
STRINGi9606.ENSP00000352271.

PTM databases

PhosphoSiteiO43543.

Proteomic databases

MaxQBiO43543.
PaxDbiO43543.
PRIDEiO43543.

Protocols and materials databases

DNASUi7516.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359321; ENSP00000352271; ENSG00000196584.
GeneIDi7516.
KEGGihsa:7516.
UCSCiuc003wld.3. human.

Organism-specific databases

CTDi7516.
GeneCardsiGC07M152341.
HGNCiHGNC:12829. XRCC2.
MIMi600375. gene.
neXtProtiNX_O43543.
Orphaneti227535. Hereditary breast cancer.
PharmGKBiPA37421.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG279678.
GeneTreeiENSGT00390000020445.
HOGENOMiHOG000004802.
HOVERGENiHBG079347.
InParanoidiO43543.
KOiK10879.
OMAiDTDYHFD.
OrthoDBiEOG7K9K4C.
PhylomeDBiO43543.
TreeFamiTF101202.

Miscellaneous databases

ChiTaRSiXRCC2. human.
GeneWikiiXRCC2.
GenomeRNAii7516.
NextBioi29411.
PROiO43543.
SOURCEiSearch...

Gene expression databases

BgeeiO43543.
CleanExiHS_XRCC2.
GenevestigatoriO43543.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013632. DNA_recomb/repair_Rad51_C.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages."
    Liu N., Lamerdin J.E., Tebbs R.S., Schild D., Tucker J.D., Shen M.R., Brookman K.W., Siciliano M.J., Walter C.A., Fan W., Narayana L.S., Zhou Z.-Q., Adamson A.W., Sorensen K.J., Chen D.J., Jones N.J., Thompson L.H.
    Mol. Cell 1:783-793(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Cervix carcinoma.
  2. "The XRCC2 DNA repair gene: identification of a positional candidate."
    Tambini C.E., George A.M., Rommens J.M., Tsui L.-C., Scherer S.W., Thacker J.
    Genomics 41:84-92(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The XRCC2 DNA repair gene from human and mouse encodes a novel member of the recA/RAD51 family."
    Cartwright R., Tambini C.E., Simpson P.J., Thacker J.
    Nucleic Acids Res. 26:3084-3089(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Cervix carcinoma.
  4. NIEHS SNPs program
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-188 AND THR-221.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  8. "Identification and purification of two distinct complexes containing the five RAD51 paralogs."
    Masson J.Y., Tarsounas M.C., Stasiak A.Z., Stasiak A., Shah R., McIlwraith M.J., Benson F.E., West S.C.
    Genes Dev. 15:3296-3307(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE BCDX2 COMPLEX WITH RAD51C; RAD51D AND XRCC2.
  9. "RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51."
    Miller K.A., Yoshikawa D.M., McConnell I.R., Clark R., Schild D., Albala J.S.
    J. Biol. Chem. 277:8406-8411(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  10. "Homologous pairing and ring and filament structure formation activities of the human Xrcc2*Rad51D complex."
    Kurumizaka H., Ikawa S., Nakada M., Enomoto R., Kagawa W., Kinebuchi T., Yamazoe M., Yokoyama S., Shibata T.
    J. Biol. Chem. 277:14315-14320(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  11. "Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells."
    Wiese C., Collins D.W., Albala J.S., Thompson L.H., Kronenberg A., Schild D.
    Nucleic Acids Res. 30:1001-1008(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH RAD51B; RAD51C AND RAD51D.
  12. "Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells."
    Liu N., Schild D., Thelen M.P., Thompson L.H.
    Nucleic Acids Res. 30:1009-1015(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH RAD51B; RAD51C AND RAD51D.
  13. "Domain mapping of the Rad51 paralog protein complexes."
    Miller K.A., Sawicka D., Barsky D., Albala J.S.
    Nucleic Acids Res. 32:169-178(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD51D.
  14. "Homologous recombination proteins are associated with centrosomes and are required for mitotic stability."
    Cappelli E., Townsend S., Griffin C., Thacker J.
    Exp. Cell Res. 317:1203-1213(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  15. "Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway."
    Chun J., Buechelmaier E.S., Powell S.N.
    Mol. Cell. Biol. 33:387-395(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE BCDX2 COMPLEX.
  16. "Ring-shaped Rad51 paralog protein complexes bind Holliday junctions and replication forks as visualized by electron microscopy."
    Compton S.A., Ozgur S., Griffith J.D.
    J. Biol. Chem. 285:13349-13356(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ELECTRON MICROSCOPY OF THE BCDX2 COMPLEX, DNA-BINDING OF THE BCDX2 COMPLEX.

Entry informationi

Entry nameiXRCC2_HUMAN
AccessioniPrimary (citable) accession number: O43543
Secondary accession number(s): B2R925
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: June 1, 1998
Last modified: March 4, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.