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O43543

- XRCC2_HUMAN

UniProt

O43543 - XRCC2_HUMAN

Protein

DNA repair protein XRCC2

Gene

XRCC2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.4 Publications

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. DNA binding Source: UniProtKB-KW
    3. DNA-dependent ATPase activity Source: InterPro
    4. protein binding Source: IntAct

    GO - Biological processi

    1. centrosome organization Source: UniProtKB
    2. DNA repair Source: BHF-UCL
    3. double-strand break repair via homologous recombination Source: UniProtKB
    4. in utero embryonic development Source: Ensembl
    5. meiotic nuclear division Source: ProtInc
    6. mitotic cell cycle Source: UniProtKB
    7. negative regulation of neuron apoptotic process Source: Ensembl
    8. positive regulation of neurogenesis Source: Ensembl
    9. response to gamma radiation Source: Ensembl
    10. response to X-ray Source: Ensembl
    11. strand invasion Source: UniProtKB

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein XRCC2
    Alternative name(s):
    X-ray repair cross-complementing protein 2
    Gene namesi
    Name:XRCC2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:12829. XRCC2.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB-KW
    3. Rad51B-Rad51C-Rad51D-XRCC2 complex Source: UniProtKB
    4. replication fork Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    Orphaneti227535. Hereditary breast cancer.
    PharmGKBiPA37421.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 280280DNA repair protein XRCC2PRO_0000122948Add
    BLAST

    Proteomic databases

    PaxDbiO43543.
    PRIDEiO43543.

    PTM databases

    PhosphoSiteiO43543.

    Expressioni

    Gene expression databases

    BgeeiO43543.
    CleanExiHS_XRCC2.
    GenevestigatoriO43543.

    Interactioni

    Subunit structurei

    Interacts with RAD51D. Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. In the absence of DNA, the BCDX2 subcomplex XRCC2:RAD51D formed a multimeric ring structure; in the presence of single-stranded DNA it formed a filamentous structure with the ssDNA.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RAD51CO435022EBI-3918457,EBI-2267048
    RAD51DO7577116EBI-3918457,EBI-1055693

    Protein-protein interaction databases

    BioGridi113350. 9 interactions.
    DIPiDIP-24242N.
    IntActiO43543. 7 interactions.
    MINTiMINT-137755.
    STRINGi9606.ENSP00000352271.

    Structurei

    3D structure databases

    ProteinModelPortaliO43543.
    SMRiO43543. Positions 9-196.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RecA family. RAD51 subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG279678.
    HOGENOMiHOG000004802.
    HOVERGENiHBG079347.
    InParanoidiO43543.
    KOiK10879.
    OMAiDTDYHFD.
    OrthoDBiEOG7K9K4C.
    PhylomeDBiO43543.
    TreeFamiTF101202.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR013632. DNA_recomb/repair_Rad51_C.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    [Graphical view]
    PfamiPF08423. Rad51. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS50162. RECA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O43543-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCSAFHRAES GTELLARLEG RSSLKEIEPN LFADEDSPVH GDILEFHGPE    50
    GTGKTEMLYH LTARCILPKS EGGLEVEVLF IDTDYHFDML RLVTILEHRL 100
    SQSSEEIIKY CLGRFFLVYC SSSTHLLLTL YSLESMFCSH PSLCLLILDS 150
    LSAFYWIDRV NGGESVNLQE STLRKCSQCL EKLVNDYRLV LFATTQTIMQ 200
    KASSSSEEPS HASRRLCDVD IDYRPYLCKA WQQLVKHRMF FSKQDDSQSS 250
    NQFSLVSRCL KSNSLKKHFF IIGESGVEFC 280
    Length:280
    Mass (Da):31,956
    Last modified:June 1, 1998 - v1
    Checksum:i5656277E74C06074
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti16 – 161A → S.
    Corresponds to variant rs4987090 [ dbSNP | Ensembl ].
    VAR_020403
    Natural varianti188 – 1881R → H.1 Publication
    Corresponds to variant rs3218536 [ dbSNP | Ensembl ].
    VAR_020404
    Natural varianti221 – 2211I → T.1 Publication
    Corresponds to variant rs3218537 [ dbSNP | Ensembl ].
    VAR_029294

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF035587 mRNA. Translation: AAC05369.1.
    AC003109 Genomic DNA. Translation: AAC05802.1.
    Y08837 mRNA. Translation: CAA70065.1.
    Y17033 Genomic DNA. Translation: CAA76597.1.
    AF520762 Genomic DNA. Translation: AAM55241.1.
    AK313607 mRNA. Translation: BAG36372.1.
    CH471173 Genomic DNA. Translation: EAW53968.1.
    BC042137 mRNA. Translation: AAH42137.1.
    CCDSiCCDS5933.1.
    RefSeqiNP_005422.1. NM_005431.1.
    UniGeneiHs.647093.

    Genome annotation databases

    EnsembliENST00000359321; ENSP00000352271; ENSG00000196584.
    GeneIDi7516.
    KEGGihsa:7516.
    UCSCiuc003wld.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF035587 mRNA. Translation: AAC05369.1 .
    AC003109 Genomic DNA. Translation: AAC05802.1 .
    Y08837 mRNA. Translation: CAA70065.1 .
    Y17033 Genomic DNA. Translation: CAA76597.1 .
    AF520762 Genomic DNA. Translation: AAM55241.1 .
    AK313607 mRNA. Translation: BAG36372.1 .
    CH471173 Genomic DNA. Translation: EAW53968.1 .
    BC042137 mRNA. Translation: AAH42137.1 .
    CCDSi CCDS5933.1.
    RefSeqi NP_005422.1. NM_005431.1.
    UniGenei Hs.647093.

    3D structure databases

    ProteinModelPortali O43543.
    SMRi O43543. Positions 9-196.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113350. 9 interactions.
    DIPi DIP-24242N.
    IntActi O43543. 7 interactions.
    MINTi MINT-137755.
    STRINGi 9606.ENSP00000352271.

    PTM databases

    PhosphoSitei O43543.

    Proteomic databases

    PaxDbi O43543.
    PRIDEi O43543.

    Protocols and materials databases

    DNASUi 7516.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000359321 ; ENSP00000352271 ; ENSG00000196584 .
    GeneIDi 7516.
    KEGGi hsa:7516.
    UCSCi uc003wld.3. human.

    Organism-specific databases

    CTDi 7516.
    GeneCardsi GC07M152341.
    HGNCi HGNC:12829. XRCC2.
    MIMi 600375. gene.
    neXtProti NX_O43543.
    Orphaneti 227535. Hereditary breast cancer.
    PharmGKBi PA37421.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG279678.
    HOGENOMi HOG000004802.
    HOVERGENi HBG079347.
    InParanoidi O43543.
    KOi K10879.
    OMAi DTDYHFD.
    OrthoDBi EOG7K9K4C.
    PhylomeDBi O43543.
    TreeFami TF101202.

    Miscellaneous databases

    ChiTaRSi XRCC2. human.
    GeneWikii XRCC2.
    GenomeRNAii 7516.
    NextBioi 29411.
    PROi O43543.
    SOURCEi Search...

    Gene expression databases

    Bgeei O43543.
    CleanExi HS_XRCC2.
    Genevestigatori O43543.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR013632. DNA_recomb/repair_Rad51_C.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    [Graphical view ]
    Pfami PF08423. Rad51. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS50162. RECA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages."
      Liu N., Lamerdin J.E., Tebbs R.S., Schild D., Tucker J.D., Shen M.R., Brookman K.W., Siciliano M.J., Walter C.A., Fan W., Narayana L.S., Zhou Z.-Q., Adamson A.W., Sorensen K.J., Chen D.J., Jones N.J., Thompson L.H.
      Mol. Cell 1:783-793(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Tissue: Cervix carcinoma.
    2. "The XRCC2 DNA repair gene: identification of a positional candidate."
      Tambini C.E., George A.M., Rommens J.M., Tsui L.-C., Scherer S.W., Thacker J.
      Genomics 41:84-92(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The XRCC2 DNA repair gene from human and mouse encodes a novel member of the recA/RAD51 family."
      Cartwright R., Tambini C.E., Simpson P.J., Thacker J.
      Nucleic Acids Res. 26:3084-3089(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Tissue: Cervix carcinoma.
    4. NIEHS SNPs program
      Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-188 AND THR-221.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    8. "Identification and purification of two distinct complexes containing the five RAD51 paralogs."
      Masson J.Y., Tarsounas M.C., Stasiak A.Z., Stasiak A., Shah R., McIlwraith M.J., Benson F.E., West S.C.
      Genes Dev. 15:3296-3307(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE BCDX2 COMPLEX WITH RAD51C; RAD51D AND XRCC2.
    9. "RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51."
      Miller K.A., Yoshikawa D.M., McConnell I.R., Clark R., Schild D., Albala J.S.
      J. Biol. Chem. 277:8406-8411(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    10. "Homologous pairing and ring and filament structure formation activities of the human Xrcc2*Rad51D complex."
      Kurumizaka H., Ikawa S., Nakada M., Enomoto R., Kagawa W., Kinebuchi T., Yamazoe M., Yokoyama S., Shibata T.
      J. Biol. Chem. 277:14315-14320(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    11. "Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells."
      Wiese C., Collins D.W., Albala J.S., Thompson L.H., Kronenberg A., Schild D.
      Nucleic Acids Res. 30:1001-1008(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH RAD51B; RAD51C AND RAD51D.
    12. "Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells."
      Liu N., Schild D., Thelen M.P., Thompson L.H.
      Nucleic Acids Res. 30:1009-1015(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH RAD51B; RAD51C AND RAD51D.
    13. "Domain mapping of the Rad51 paralog protein complexes."
      Miller K.A., Sawicka D., Barsky D., Albala J.S.
      Nucleic Acids Res. 32:169-178(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAD51D.
    14. "Homologous recombination proteins are associated with centrosomes and are required for mitotic stability."
      Cappelli E., Townsend S., Griffin C., Thacker J.
      Exp. Cell Res. 317:1203-1213(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    15. "Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway."
      Chun J., Buechelmaier E.S., Powell S.N.
      Mol. Cell. Biol. 33:387-395(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE BCDX2 COMPLEX.
    16. "Ring-shaped Rad51 paralog protein complexes bind Holliday junctions and replication forks as visualized by electron microscopy."
      Compton S.A., Ozgur S., Griffith J.D.
      J. Biol. Chem. 285:13349-13356(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: ELECTRON MICROSCOPY OF THE BCDX2 COMPLEX, DNA-BINDING OF THE BCDX2 COMPLEX.

    Entry informationi

    Entry nameiXRCC2_HUMAN
    AccessioniPrimary (citable) accession number: O43543
    Secondary accession number(s): B2R925
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2002
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3