O43543 (XRCC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair protein XRCC2

Alternative name(s):
X-ray repair cross-complementing protein 2

Gene names

Name:

XRCC2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Ref.8
Subunit structure	Interacts with RAD51D. Part of a BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2. Part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3. In the absence of DNA, XRCC2-RAD51D formed a multimeric ring structure. In the presence of single-stranded DNA, XRCC2-RAD51D formed a filamentous structure. Ref.8 Ref.9 Ref.10 Ref.11
Subcellular location	Nucleus.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the RecA family. RAD51 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from genetic interaction PubMed 10422536. Source: BHF-UCL double-strand break repair via homologous recombination Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from electronic annotation. Source: Compara meiosis Traceable author statement Ref.3. Source: ProtInc negative regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of neurogenesis Inferred from electronic annotation. Source: Compara response to X-ray Inferred from electronic annotation. Source: Compara response to gamma radiation Inferred from electronic annotation. Source: Compara
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	DNA repair protein XRCC2		PRO_0000122948
Natural variations
	Natural variant	16	1	A → S. Corresponds to variant rs4987090 [ dbSNP | Ensembl ].		VAR_020403
	Natural variant	188	1	R → H. Ref.4 Corresponds to variant rs3218536 [ dbSNP | Ensembl ].		VAR_020404
	Natural variant	221	1	I → T. Ref.4 Corresponds to variant rs3218537 [ dbSNP | Ensembl ].		VAR_029294

Sequences

	Sequence		Length	Mass (Da)
	O43543 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 5656277E74C06074	FASTA	280	31,956
	10 20 30 40 50 60 MCSAFHRAES GTELLARLEG RSSLKEIEPN LFADEDSPVH GDILEFHGPE GTGKTEMLYH 70 80 90 100 110 120 LTARCILPKS EGGLEVEVLF IDTDYHFDML RLVTILEHRL SQSSEEIIKY CLGRFFLVYC 130 140 150 160 170 180 SSSTHLLLTL YSLESMFCSH PSLCLLILDS LSAFYWIDRV NGGESVNLQE STLRKCSQCL 190 200 210 220 230 240 EKLVNDYRLV LFATTQTIMQ KASSSSEEPS HASRRLCDVD IDYRPYLCKA WQQLVKHRMF 250 260 270 280 FSKQDDSQSS NQFSLVSRCL KSNSLKKHFF IIGESGVEFC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages." Liu N., Lamerdin J.E., Tebbs R.S., Schild D., Tucker J.D., Shen M.R., Brookman K.W., Siciliano M.J., Walter C.A., Fan W., Narayana L.S., Zhou Z.-Q., Adamson A.W., Sorensen K.J., Chen D.J., Jones N.J., Thompson L.H. Mol. Cell 1:783-793(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Cervix carcinoma.
[2]	"The XRCC2 DNA repair gene: identification of a positional candidate." Tambini C.E., George A.M., Rommens J.M., Tsui L.-C., Scherer S.W., Thacker J. Genomics 41:84-92(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The XRCC2 DNA repair gene from human and mouse encodes a novel member of the recA/RAD51 family." Cartwright R., Tambini C.E., Simpson P.J., Thacker J. Nucleic Acids Res. 26:3084-3089(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Cervix carcinoma.
[4]	NIEHS SNPs program Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS HIS-188 AND THR-221.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[8]	"Identification and purification of two distinct complexes containing the five RAD51 paralogs." Masson J.Y., Tarsounas M.C., Stasiak A.Z., Stasiak A., Shah R., McIlwraith M.J., Benson F.E., West S.C. Genes Dev. 15:3296-3307(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH RAD51C; RAD51D AND XRCC2.
[9]	"RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51." Miller K.A., Yoshikawa D.M., McConnell I.R., Clark R., Schild D., Albala J.S. J. Biol. Chem. 277:8406-8411(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH RAD51B; RAD51C; RAD51D AND XRCC3.
[10]	"Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells." Wiese C., Collins D.W., Albala J.S., Thompson L.H., Kronenberg A., Schild D. Nucleic Acids Res. 30:1001-1008(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH RAD51B; RAD51C AND RAD51D.
[11]	"Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells." Liu N., Schild D., Thelen M.P., Thompson L.H. Nucleic Acids Res. 30:1009-1015(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH RAD51B; RAD51C AND RAD51D.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF035587 mRNA. Translation: AAC05369.1. AC003109 Genomic DNA. Translation: AAC05802.1. Y08837 mRNA. Translation: CAA70065.1. Y17033 Genomic DNA. Translation: CAA76597.1. AF520762 Genomic DNA. Translation: AAM55241.1. AK313607 mRNA. Translation: BAG36372.1. CH471173 Genomic DNA. Translation: EAW53968.1. BC042137 mRNA. Translation: AAH42137.1.
IPI	IPI00306229.
RefSeq	NP_005422.1. NM_005431.1.
UniGene	Hs.647093.
3D structure databases
ProteinModelPortal	O43543.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24242N.
IntAct	O43543. 2 interactions.
MINT	MINT-137755.
STRING	9606.ENSP00000352271.
PTM databases
PhosphoSite	O43543.
Proteomic databases
PaxDb	O43543.
PRIDE	O43543.
Protocols and materials databases
DNASU	7516.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359321; ENSP00000352271; ENSG00000196584.
GeneID	7516.
KEGG	hsa:7516.
UCSC	uc003wld.3. human.
Organism-specific databases
CTD	7516.
GeneCards	GC07M152341.
HGNC	HGNC:12829. XRCC2.
MIM	600375. gene.
neXtProt	NX_O43543.
Orphanet	227535. Hereditary breast cancer.
PharmGKB	PA37421.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG279678.
HOGENOM	HOG000004802.
HOVERGEN	HBG079347.
InParanoid	O43543.
KO	K10879.
OMA	DTDYHFD.
OrthoDB	EOG4X6C91.
PhylomeDB	O43543.
Gene expression databases
Bgee	O43543.
CleanEx	HS_XRCC2.
Genevestigator	O43543.
GermOnline	ENSG00000196584. Homo sapiens.
Family and domain databases
InterPro	IPR013632. DNA_recomb/repair_Rad51_C. IPR020588. DNA_recomb_RecA/RadB_ATP-bd. [Graphical view]
Pfam	PF08423. Rad51. 1 hit. [Graphical view]
PROSITE	PS50162. RECA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	XRCC2. human.
GenomeRNAi	7516.
NextBio	29411.
SOURCE	Search...

Entry information

Entry name

XRCC2_HUMAN

Accession

Primary (citable) accession number: O43543
Secondary accession number(s): B2R925

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.