Reviewed, UniProtKB/Swiss-Prot O43542 (XRCC3_HUMAN)

Names and origin

Protein names

Recommended name:
    DNA repair protein XRCC3
Alternative name(s):
    X-ray repair cross-complementing protein 3

Gene names

Name:

XRCC3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions.
Subunit structure	Interacts with RAD51C and RAD51. Part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3.
Subcellular location	NucleusProbable.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Involvement in disease	Met-241 is associated with the development of cutaneous malignant melanoma.
Sequence similarities	Belongs to the recA family. RAD51 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Disease	Disease mutation
Ligand	ATP-binding DNA-binding Nucleotide-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA recombination Ref.1 Traceable author statement. Source: ProtInc DNA repair Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-dependent ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	DNA repair protein XRCC3		PRO_0000122951
Regions
	Nucleotide binding	107 – 114	8	ATP Potential
Amino acid modifications
	Modified residue	225	1	Phosphoserine
Natural variations
	Natural variant	94	1	R → H: dbSNP rs3212057.		VAR_020405
	Natural variant	241	1	T → M Associated with cutaneous malignant melanoma. dbSNP rs861539.		VAR_013006
	Natural variant	271	1	G → R: dbSNP rs28903080.		VAR_029295
	Natural variant	302	1	R → H: dbSNP rs28903081.		VAR_029296

Sequences

	Sequence		Length	Mass (Da)
	O43542-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 4DDF3B163C3B3332	FASTA	346	37,850
	10 20 30 40 50 60 MDLDLLDLNP RIIAAIKKAK LKSVKEVLHF SGPDLKRLTN LSSPEVWHLL RTASLHLRGS 70 80 90 100 110 120 SILTALQLHQ QKERFPTQHQ RLSLGCPVLD ALLRGGLPLD GITELAGRSS AGKTQLALQL 130 140 150 160 170 180 CLAVQFPRQH GGLEAGAVYI CTEDAFPHKR LQQLMAQQPR LRTDVPGELL QKLRFGSQIF 190 200 210 220 230 240 IEHVADVDTL LECVNKKVPV LLSRGMARLV VIDSVAAPFR CEFDSQASAP RARHLQSLGA 250 260 270 280 290 300 TLRELSSAFQ SPVLCINQVT EAMEEQGAAH GPLGFWDERV SPALGITWAN QLLVRLLADR 310 320 330 340 LREEEAALGC PARTLRVLSA PHLPPSSCSY TISAEGVRGT PGTQSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages." Liu N., Lamerdin J.E., Tebbs R.S., Schild D., Tucker J.D., Shen M.R., Brookman K.W., Siciliano M.J., Walter C.A., Fan W., Narayana L.S., Zhou Z.-Q., Adamson A.W., Sorensen K.J., Chen D.J., Jones N.J., Thompson L.H. Mol. Cell 1:783-793(1998) [PubMed: 9660962] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT MET-241. Tissue: Cervix carcinoma.
[2]	"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Rieder M.J., Livingston R.J., Braun A.C., Montoya M.A., Chung M.-W., Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph, Muscle and Placenta.
[4]	"RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51." Miller K.A., Yoshikawa D.M., McConnell I.R., Clark R., Schild D., Albala J.S. J. Biol. Chem. 277:8406-8411(2002) [PubMed: 11744692] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH RAD51B; RAD51C; RAD51D AND XRCC3.
[5]	"Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells." Liu N., Schild D., Thelen M.P., Thompson L.H. Nucleic Acids Res. 30:1009-1015(2002) [PubMed: 11842113] [Abstract] Cited for: INTERACTION WITH RAD51 AND RAD51C.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, MASS SPECTROMETRY.
[7]	"A variant within the DNA repair gene XRCC3 is associated with the development of melanoma skin cancer." Winsey S.L., Haldar N.A., Marsh H.P., Bunce M., Marshall S.E., Harris A.L., Wojnarowska F., Welsh K.I. Cancer Res. 60:5612-5616(2000) [PubMed: 11059748] [Abstract] Cited for: VARIANT MET-241.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF035586 mRNA. Translation: AAC05368.1. AF037222 Genomic DNA. Translation: AAC04805.1. AF508041 Genomic DNA. Translation: AAM23015.1. BC001036 mRNA. Translation: AAH01036.1. BC002949 mRNA. Translation: AAH02949.1. BC011725 mRNA. Translation: AAH11725.1.
RefSeq	NP_001093588.1. NP_001093589.1. NP_005423.1.
UniGene	Hs.592325
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	O43542.
Polymorphism databases
NIEHS-SNPs	Search...
Genome annotation databases
Ensembl	ENSG00000126215. Homo sapiens. [Contig view]
GeneID	7517.
KEGG	hsa:7517.
Organism-specific databases
H-InvDB	HIX0012007.
HGNC	HGNC:12830. XRCC3.
MIM	600675. gene.
PharmGKB	PA37422.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O43542.
HOVERGEN	O43542.
Gene expression databases
ArrayExpress	O43542.
CleanEx	HS_XRCC3.
GermOnline	ENSG00000126215. Homo sapiens.
Family and domain databases
InterPro	IPR016467. DNA_repair_recomb_RecA-like. IPR013632. Rad51_C. IPR001553. RecA_bac. [Graphical view]
Pfam	PF08423. Rad51. 1 hit. [Graphical view]
PIRSF	PIRSF005856. Rad51. 1 hit.
PROSITE	PS50162. RECA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	29415.
SOURCE	Search...

Entry information

Entry name

XRCC3_HUMAN

Accession

Primary (citable) accession number: O43542
Secondary accession number(s): O43568, Q9BU18

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	June 1, 1998
Last modified:	November 25, 2008
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)