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O43529

- CHSTA_HUMAN

UniProt

O43529 - CHSTA_HUMAN

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Protein

Carbohydrate sulfotransferase 10

Gene
CHST10
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi127 – 1337PAPS Inferred
Nucleotide bindingi189 – 1979PAPS Inferred

GO - Molecular functioni

  1. HNK-1 sulfotransferase activity Source: Ensembl
  2. sulfotransferase activity Source: ProtInc

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. cell adhesion Source: ProtInc
  3. learning Source: Ensembl
  4. long-term memory Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 10 (EC:2.8.2.-)
Alternative name(s):
HNK-1 sulfotransferase
Short name:
HNK-1ST
Short name:
HNK1ST
Short name:
HuHNK-1ST
Gene namesi
Name:CHST10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:19650. CHST10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Cytoplasmic Reviewed prediction
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini28 – 356329Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: ProtInc
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi128 – 1281K → A: Loss of function. 1 Publication
Mutagenesisi128 – 1281K → R: Induces a reduction in enzyme activity. 1 Publication
Mutagenesisi189 – 1891R → A or K: Loss of function. 1 Publication
Mutagenesisi190 – 1901D → A: Loss of function. 1 Publication
Mutagenesisi190 – 1901D → E: Induces a mild reduction in enzyme activity. 1 Publication
Mutagenesisi191 – 1911P → A or G: Loss of function. 1 Publication
Mutagenesisi197 – 1971S → A or T: Loss of function. 1 Publication

Organism-specific databases

PharmGKBiPA134920179.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Carbohydrate sulfotransferase 10PRO_0000189657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi228 – 2281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi316 – 3161N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO43529.
PRIDEiO43529.

PTM databases

PhosphoSiteiO43529.

Expressioni

Tissue specificityi

In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at higher level in the frontal lobe.1 Publication

Gene expression databases

ArrayExpressiO43529.
BgeeiO43529.
CleanExiHS_CHST10.
GenevestigatoriO43529.

Organism-specific databases

HPAiHPA012884.
HPA051545.

Interactioni

Protein-protein interaction databases

BioGridi114868. 3 interactions.
IntActiO43529. 1 interaction.
STRINGi9606.ENSP00000264249.

Structurei

3D structure databases

ProteinModelPortaliO43529.
SMRiO43529. Positions 176-203.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG86863.
HOVERGENiHBG050951.
InParanoidiO43529.
KOiK09674.
PhylomeDBiO43529.
TreeFamiTF325581.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43529-1 [UniParc]FASTAAdd to Basket

« Hide

MHHQWLLLAA CFWVIFMFMV ASKFITLTFK DPDVYSAKQE FLFLTTMPEV    50
RKLPEEKHIP EELKPTGKEL PDSQLVQPLV YMERLELIRN VCRDDALKNL 100
SHTPVSKFVL DRIFVCDKHK ILFCQTPKVG NTQWKKVLIV LNGAFSSIEE 150
IPENVVHDHE KNGLPRLSSF SDAEIQKRLK TYFKFFIVRD PFERLISAFK 200
DKFVHNPRFE PWYRHEIAPG IIRKYRRNRT ETRGIQFEDF VRYLGDPNHR 250
WLDLQFGDHI IHWVTYVELC APCEIMYSVI GHHETLEDDA PYILKEAGID 300
HLVSYPTIPP GITVYNRTKV EHYFLGISKR DIRRLYARFE GDFKLFGYQK 350
PDFLLN 356
Length:356
Mass (Da):42,207
Last modified:June 1, 1998 - v1
Checksum:i0AE82883CCD8291A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti20 – 201V → L.
Corresponds to variant rs35177621 [ dbSNP | Ensembl ].
VAR_033737
Natural varianti258 – 2581D → N.
Corresponds to variant rs3748932 [ dbSNP | Ensembl ].
VAR_021470

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF033827 mRNA. Translation: AAC04707.1.
AF070594 mRNA. Translation: AAC28651.1.
AK313241 mRNA. Translation: BAG36052.1.
AC012493 Genomic DNA. Translation: AAX93044.1.
CH471127 Genomic DNA. Translation: EAX01841.1.
CH471127 Genomic DNA. Translation: EAX01842.1.
CH471127 Genomic DNA. Translation: EAX01843.1.
CH471127 Genomic DNA. Translation: EAX01844.1.
BC010441 mRNA. Translation: AAH10441.1.
CCDSiCCDS2047.1.
RefSeqiNP_004845.1. NM_004854.4.
UniGeneiHs.516370.
Hs.731724.

Genome annotation databases

EnsembliENST00000264249; ENSP00000264249; ENSG00000115526.
ENST00000409701; ENSP00000387309; ENSG00000115526.
GeneIDi9486.
KEGGihsa:9486.
UCSCiuc002tam.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF033827 mRNA. Translation: AAC04707.1 .
AF070594 mRNA. Translation: AAC28651.1 .
AK313241 mRNA. Translation: BAG36052.1 .
AC012493 Genomic DNA. Translation: AAX93044.1 .
CH471127 Genomic DNA. Translation: EAX01841.1 .
CH471127 Genomic DNA. Translation: EAX01842.1 .
CH471127 Genomic DNA. Translation: EAX01843.1 .
CH471127 Genomic DNA. Translation: EAX01844.1 .
BC010441 mRNA. Translation: AAH10441.1 .
CCDSi CCDS2047.1.
RefSeqi NP_004845.1. NM_004854.4.
UniGenei Hs.516370.
Hs.731724.

3D structure databases

ProteinModelPortali O43529.
SMRi O43529. Positions 176-203.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114868. 3 interactions.
IntActi O43529. 1 interaction.
STRINGi 9606.ENSP00000264249.

PTM databases

PhosphoSitei O43529.

Proteomic databases

PaxDbi O43529.
PRIDEi O43529.

Protocols and materials databases

DNASUi 9486.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000264249 ; ENSP00000264249 ; ENSG00000115526 .
ENST00000409701 ; ENSP00000387309 ; ENSG00000115526 .
GeneIDi 9486.
KEGGi hsa:9486.
UCSCi uc002tam.3. human.

Organism-specific databases

CTDi 9486.
GeneCardsi GC02M101008.
HGNCi HGNC:19650. CHST10.
HPAi HPA012884.
HPA051545.
MIMi 606376. gene.
neXtProti NX_O43529.
PharmGKBi PA134920179.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG86863.
HOVERGENi HBG050951.
InParanoidi O43529.
KOi K09674.
PhylomeDBi O43529.
TreeFami TF325581.

Miscellaneous databases

GeneWikii CHST10.
GenomeRNAii 9486.
NextBioi 35544.
PROi O43529.
SOURCEi Search...

Gene expression databases

ArrayExpressi O43529.
Bgeei O43529.
CleanExi HS_CHST10.
Genevestigatori O43529.

Family and domain databases

InterProi IPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view ]
PANTHERi PTHR12137. PTHR12137. 1 hit.
Pfami PF03567. Sulfotransfer_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning of a human sulfotransferase that directs the synthesis of the HNK-1 glycan on the neural cell adhesion molecule and glycolipids."
    Ong E., Yeh J.-C., Ding Y., Hindsgaul O., Fukuda M.
    J. Biol. Chem. 273:5190-5195(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. Yu W., Gibbs R.A.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  7. "Structure and function of HNK-1 sulfotransferase. Identification of donor and acceptor binding sites by site-directed mutagenesis."
    Ong E., Yeh J.-C., Ding Y., Hindsgaul O., Pedersen L.C., Negishi M., Fukuda M.
    J. Biol. Chem. 274:25608-25612(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-128; ARG-189; ASP-190; PRO-191 AND SER-197.

Entry informationi

Entry nameiCHSTA_HUMAN
AccessioniPrimary (citable) accession number: O43529
Secondary accession number(s): Q53T18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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