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Protein

Carbohydrate sulfotransferase 10

Gene

CHST10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 133PAPSCurated7
Nucleotide bindingi189 – 197PAPSCurated9

GO - Molecular functioni

  • HNK-1 sulfotransferase activity Source: Reactome
  • sulfotransferase activity Source: ProtInc

GO - Biological processi

  • carbohydrate biosynthetic process Source: InterPro
  • cell adhesion Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115526-MONOMER.
ReactomeiR-HSA-975578. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 10 (EC:2.8.2.-)
Alternative name(s):
HNK-1 sulfotransferase
Short name:
HNK-1ST
Short name:
HNK1ST
Short name:
HuHNK-1ST
Gene namesi
Name:CHST10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:19650. CHST10.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 356LumenalSequence analysisAdd BLAST329

GO - Cellular componenti

  • Golgi apparatus Source: ProtInc
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi128K → A: Loss of function. 1 Publication1
Mutagenesisi128K → R: Induces a reduction in enzyme activity. 1 Publication1
Mutagenesisi189R → A or K: Loss of function. 1 Publication1
Mutagenesisi190D → A: Loss of function. 1 Publication1
Mutagenesisi190D → E: Induces a mild reduction in enzyme activity. 1 Publication1
Mutagenesisi191P → A or G: Loss of function. 1 Publication1
Mutagenesisi197S → A or T: Loss of function. 1 Publication1

Organism-specific databases

DisGeNETi9486.
OpenTargetsiENSG00000115526.
PharmGKBiPA134920179.

Polymorphism and mutation databases

BioMutaiCHST10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896571 – 356Carbohydrate sulfotransferase 10Add BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi228N-linked (GlcNAc...)Sequence analysis1
Glycosylationi316N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO43529.
PeptideAtlasiO43529.
PRIDEiO43529.

PTM databases

iPTMnetiO43529.
PhosphoSitePlusiO43529.

Expressioni

Tissue specificityi

In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at higher level in the frontal lobe.1 Publication

Gene expression databases

BgeeiENSG00000115526.
CleanExiHS_CHST10.
ExpressionAtlasiO43529. baseline and differential.
GenevisibleiO43529. HS.

Organism-specific databases

HPAiHPA012884.
HPA051545.

Interactioni

Protein-protein interaction databases

BioGridi114868. 19 interactors.
IntActiO43529. 1 interactor.
STRINGi9606.ENSP00000264249.

Structurei

3D structure databases

ProteinModelPortaliO43529.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOVERGENiHBG050951.
InParanoidiO43529.
KOiK09674.
OMAiVEHYFLG.
OrthoDBiEOG091G0FZO.
PhylomeDBiO43529.
TreeFamiTF325581.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHHQWLLLAA CFWVIFMFMV ASKFITLTFK DPDVYSAKQE FLFLTTMPEV
60 70 80 90 100
RKLPEEKHIP EELKPTGKEL PDSQLVQPLV YMERLELIRN VCRDDALKNL
110 120 130 140 150
SHTPVSKFVL DRIFVCDKHK ILFCQTPKVG NTQWKKVLIV LNGAFSSIEE
160 170 180 190 200
IPENVVHDHE KNGLPRLSSF SDAEIQKRLK TYFKFFIVRD PFERLISAFK
210 220 230 240 250
DKFVHNPRFE PWYRHEIAPG IIRKYRRNRT ETRGIQFEDF VRYLGDPNHR
260 270 280 290 300
WLDLQFGDHI IHWVTYVELC APCEIMYSVI GHHETLEDDA PYILKEAGID
310 320 330 340 350
HLVSYPTIPP GITVYNRTKV EHYFLGISKR DIRRLYARFE GDFKLFGYQK

PDFLLN
Length:356
Mass (Da):42,207
Last modified:June 1, 1998 - v1
Checksum:i0AE82883CCD8291A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03373720V → L.Corresponds to variant rs35177621dbSNPEnsembl.1
Natural variantiVAR_021470258D → N.Corresponds to variant rs3748932dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033827 mRNA. Translation: AAC04707.1.
AF070594 mRNA. Translation: AAC28651.1.
AK313241 mRNA. Translation: BAG36052.1.
AC012493 Genomic DNA. Translation: AAX93044.1.
CH471127 Genomic DNA. Translation: EAX01841.1.
CH471127 Genomic DNA. Translation: EAX01842.1.
CH471127 Genomic DNA. Translation: EAX01843.1.
CH471127 Genomic DNA. Translation: EAX01844.1.
BC010441 mRNA. Translation: AAH10441.1.
CCDSiCCDS2047.1.
RefSeqiNP_004845.1. NM_004854.4.
XP_011510509.1. XM_011512207.1.
XP_011510510.1. XM_011512208.1.
XP_011510512.1. XM_011512210.1.
XP_011510513.1. XM_011512211.1.
XP_011510514.1. XM_011512212.1.
XP_016860869.1. XM_017005380.1.
XP_016860870.1. XM_017005381.1.
XP_016860871.1. XM_017005382.1.
XP_016860872.1. XM_017005383.1.
UniGeneiHs.516370.
Hs.731724.

Genome annotation databases

EnsembliENST00000264249; ENSP00000264249; ENSG00000115526.
ENST00000409701; ENSP00000387309; ENSG00000115526.
GeneIDi9486.
KEGGihsa:9486.
UCSCiuc002tam.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033827 mRNA. Translation: AAC04707.1.
AF070594 mRNA. Translation: AAC28651.1.
AK313241 mRNA. Translation: BAG36052.1.
AC012493 Genomic DNA. Translation: AAX93044.1.
CH471127 Genomic DNA. Translation: EAX01841.1.
CH471127 Genomic DNA. Translation: EAX01842.1.
CH471127 Genomic DNA. Translation: EAX01843.1.
CH471127 Genomic DNA. Translation: EAX01844.1.
BC010441 mRNA. Translation: AAH10441.1.
CCDSiCCDS2047.1.
RefSeqiNP_004845.1. NM_004854.4.
XP_011510509.1. XM_011512207.1.
XP_011510510.1. XM_011512208.1.
XP_011510512.1. XM_011512210.1.
XP_011510513.1. XM_011512211.1.
XP_011510514.1. XM_011512212.1.
XP_016860869.1. XM_017005380.1.
XP_016860870.1. XM_017005381.1.
XP_016860871.1. XM_017005382.1.
XP_016860872.1. XM_017005383.1.
UniGeneiHs.516370.
Hs.731724.

3D structure databases

ProteinModelPortaliO43529.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114868. 19 interactors.
IntActiO43529. 1 interactor.
STRINGi9606.ENSP00000264249.

PTM databases

iPTMnetiO43529.
PhosphoSitePlusiO43529.

Polymorphism and mutation databases

BioMutaiCHST10.

Proteomic databases

PaxDbiO43529.
PeptideAtlasiO43529.
PRIDEiO43529.

Protocols and materials databases

DNASUi9486.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264249; ENSP00000264249; ENSG00000115526.
ENST00000409701; ENSP00000387309; ENSG00000115526.
GeneIDi9486.
KEGGihsa:9486.
UCSCiuc002tam.4. human.

Organism-specific databases

CTDi9486.
DisGeNETi9486.
GeneCardsiCHST10.
HGNCiHGNC:19650. CHST10.
HPAiHPA012884.
HPA051545.
MIMi606376. gene.
neXtProtiNX_O43529.
OpenTargetsiENSG00000115526.
PharmGKBiPA134920179.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOVERGENiHBG050951.
InParanoidiO43529.
KOiK09674.
OMAiVEHYFLG.
OrthoDBiEOG091G0FZO.
PhylomeDBiO43529.
TreeFamiTF325581.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115526-MONOMER.
ReactomeiR-HSA-975578. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

ChiTaRSiCHST10. human.
GeneWikiiCHST10.
GenomeRNAii9486.
PROiO43529.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115526.
CleanExiHS_CHST10.
ExpressionAtlasiO43529. baseline and differential.
GenevisibleiO43529. HS.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHSTA_HUMAN
AccessioniPrimary (citable) accession number: O43529
Secondary accession number(s): Q53T18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.