Reviewed, UniProtKB/Swiss-Prot O43521 (BIM_HUMAN)

Names and origin

Protein names

Recommended name:
    Bcl-2-like protein 11
Alternative name(s):
    Bcl2-interacting mediator of cell death

Gene names

Name:	BCL2L11
Synonyms:	BIM

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Induces apoptosis. Isoform BimL is more potent than isoform BimEL.
Subunit structure	Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity.
Subcellular location	Membrane; Peripheral membrane protein. Note= Associated with intracytoplasmic membranes By similarity.
Domain	The BH3 motif is required for Bcl-2 binding and cytotoxicity.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	activation of pro-apoptotic gene products Inferred from Experiment. Source: Reactome induction of apoptosis Traceable author statement. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome membrane fraction Ref.1 Traceable author statement. Source: ProtInc mitochondrial outer membrane Inferred from Experiment. Source: Reactome plasma membrane Inferred from Experiment. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AKT1	P31749	1	EBI-526416,EBI-296087
BCL2	P10415	1	EBI-526406,EBI-77694
BCL2	P10415	2	EBI-526420,EBI-77694
BCL2	P10415	1	EBI-526416,EBI-77694
Bcl2a1	Q07440	1	EBI-526406,EBI-707754	From a different organism.
BCL2L1	Q07817-1	4	EBI-526406,EBI-287195
BCL2L1	Q07817-1	1	EBI-526416,EBI-287195
BCL2L2	Q92843	1	EBI-526406,EBI-707714
DYNLRB1	Q9NP97	1	EBI-526406,EBI-372128
Mapk1	P63085	1	EBI-526416,EBI-397697	From a different organism.
Mcl1	P97287	3	EBI-526406,EBI-707292	From a different organism.
YWHAB	P31946	1	EBI-526406,EBI-359815

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform BimEL (identifier: O43521-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform BimL (identifier: O43521-2)

The sequence of this isoform differs from the canonical sequence as follows:
     42-101: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 198

198

Bcl-2-like protein 11

Regions

Motif

148 – 162

15

BH3

Amino acid modifications

Modified residue

77

1

Phosphoserine

Natural variations

Alternative sequence

42 – 101

60

Missing in isoform BimL.

Secondary structure

1

.

.

.

198

Helix Strand Turn

Details...

Helix

144 – 164

21

Sequences

	Sequence		Length	Mass (Da)
	Isoform BimEL [UniParc]. Last modified June 1, 1998. Version 1. Checksum: D75735E469CA6997	FASTA	198	22,171
	10 20 30 40 50 60 MAKQPSDVSS ECDREGRQLQ PAERPPQLRP GAPTSLQTEP QGNPEGNHGG EGDSCPHGSP 70 80 90 100 110 120 QGPLAPPASP GPFATRSPLF IFMRRSSLLS RSSSGYFSFD TDRSPAPMSC DKSTQTPSPP 130 140 150 160 170 180 CQAFNHYLSA MASMRQAEPA DMRPEIWIAQ ELRRIGDEFN AYYARRVFLN NYQAAEDHPR 190 MVILRLLRYI VRLVWRMH
	Isoform BimL [UniParc]. Checksum: 8BB4AAE06CB080FA		138	15,967
	10 20 30 40 50 60 MAKQPSDVSS ECDREGRQLQ PAERPPQLRP GAPTSLQTEP QDRSPAPMSC DKSTQTPSPP 70 80 90 100 110 120 CQAFNHYLSA MASMRQAEPA DMRPEIWIAQ ELRRIGDEFN AYYARRVFLN NYQAAEDHPR 130 MVILRLLRYI VRLVWRMH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bim: a novel member of the Bcl-2 family that promotes apoptosis." O'Connor L., Strasser A., O'Reilly L.A., Hausmann G., Adams J.M., Cory S., Huang D.C.S. EMBO J. 17:384-395(1998) [PubMed: 9430630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BIMEL AND BIML), FUNCTION. Tissue: Peripheral blood and Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BIMEL). Tissue: Blood.
[3]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF032457 mRNA. Translation: AAC39593.1.
AF032458 mRNA. Translation: AAC39594.1.
BC033694 mRNA. Translation: AAH33694.1.

RefSeq

NP_006529.1.
NP_619527.1.

UniGene

Hs.469658

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2NL9	X-ray	1.55	B	141-166	[»]
2VM6	X-ray	2.20	B	141-165	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:29185N.

IntAct

O43521.

PTM databases

PhosphoSite

O43521.

Genome annotation databases

Ensembl

ENSG00000153094. Homo sapiens. [Contig view]

GeneID

10018.

KEGG

hsa:10018.

Organism-specific databases

HGNC

HGNC:994. BCL2L11.

HPA

CAB002616.

MIM

603827. gene.

PharmGKB

PA25305.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

O43521.

HOVERGEN

O43521.

Enzyme and pathway databases

Reactome

REACT_578. Apoptosis.

Gene expression databases

ArrayExpress

O43521.

CleanEx

HS_BCL2L11.

GermOnline

ENSG00000153094. Homo sapiens.

Family and domain databases

InterPro

IPR017288. Apoptosis_Bcl-2-like_11.
IPR014771. Apoptosis_Bim_N.
IPR000712. Bcl2_BH.
IPR015040. Bclx_interact.
[Graphical view]

Pfam

PF08945. Bclx_interact. 1 hit.
PF06773. Bim_N. 1 hit.
[Graphical view]

PIRSF

PIRSF037827. Bcl-2-like_p11. 1 hit.

PROSITE

PS01259. BH3. False negative.
[Graphical view]

ProDom

O43521.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

LinkHub

O43521.

SOURCE

Search...

ProtoNet

Search...

Entry information

Entry name

BIM_HUMAN

Accession

Primary (citable) accession number: O43521
Secondary accession number(s): O43522

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	June 1, 1998
Last modified:	July 22, 2008
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)