WAS/WASL-interacting protein family member 1

Names and origin

Protein names

Recommended name:
    WAS/WASL-interacting protein family member 1
Alternative name(s):
    Wiskott-Aldrich syndrome protein-interacting protein
      Short name=WASP-interacting protein
    Protein PRPL-2

Gene names

Name:	WIPF1
Synonyms:	WASPIP, WIP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	May have direct activity on the actin cytoskeleton. Induces actin polymerization and redistribution. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation By similarity. Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
Subunit structure	Binds to WAS, profilin and actin. Binds to WASL By similarity.
Subcellular location	Cytoplasmic vesicleBy similarity. Cytoplasm › cytoskeletonBy similarity. Note= Vesicle surfaces and along actin tails. Co-localized with actin stress fibers. When co-expressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL By similarity.
Tissue specificity	Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
Domain	Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.
Miscellaneous	Recruited to PIP5K-induced vesicle surfaces in the absence of functional WASL By similarity.
Sequence similarities	Belongs to the verprolin family. Contains 1 WH2 domain.

Hide | Top

Ontologies

Keywords
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	actin polymerization or depolymerization Ref.1 Traceable author statement. Source: ProtInc protein complex assembly Ref.1 Traceable author statement. Source: ProtInc
Cellular component	actin cytoskeleton Ref.1 Traceable author statement. Source: ProtInc cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	actin binding Ref.1 Traceable author statement. Source: ProtInc profilin binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTTN	Q14247	1	EBI-346356,EBI-351886
Cttn	Q60598	2	EBI-346356,EBI-397955	From a different organism.
FYN	P06241	1	EBI-346356,EBI-515315
HCK	P08631	3	EBI-346356,EBI-346340
NCK1	P16333	2	EBI-346356,EBI-389883
WAS	P42768	6	EBI-346356,EBI-346375

Hide | Top

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43516-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43516-2) The sequence of this isoform differs from the canonical sequence as follows: 487-503: GSNRRERGAPPLPPIPR → EYFCQGF

Isoform 3 (identifier: O43516-3) The sequence of this isoform differs from the canonical sequence as follows: 503-503: R → RPPKQAAE
Notes: No experimental confirmation available.

Isoform 4 (identifier: O43516-4) The sequence of this isoform differs from the canonical sequence as follows: 220-364: Missing.
Notes: No experimental confirmation available.

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 503

503

WAS/WASL-interacting protein family member 1

PRO_0000065941

Regions

Domain

32 – 49

18

WH2

Repeat

352 – 361

10

XRSGPXPPXP motif 1

Repeat

374 – 383

10

XRSGPXPPXP motif 2

Repeat

410 – 419

10

XRSGPXPPXP motif 3

Region

45 – 48

4

Binds actin

Compositional bias

2 – 13

12

Poly-Pro

Compositional bias

64 – 96

33

Gly-rich

Compositional bias

241 – 244

4

Poly-Ser

Compositional bias

264 – 433

170

Pro-rich

Amino acid modifications

Modified residue

350

1

Phosphoserine

Natural variations

Alternative sequence

220 – 364

145

Missing in isoform 4.

VSP_012964

Alternative sequence

487 – 503

17

GSNRR…PPIPR → EYFCQGF in isoform 2.

VSP_012965

Alternative sequence

503

1

R → RPPKQAAE in isoform 3.

VSP_012966

Natural variant

198

1

L → P: dbSNP rs4972450.

VAR_046526

Natural variant

495

1

A → G

VAR_010295

Experimental info

Sequence conflict

119

1

N → D in CAE45928. Ref.3

Sequence conflict

120

1

D → H in AAH02914. Ref.5

Sequence conflict

303 – 309

7

SSQAPPP → PHRPHLR in AAC03767. Ref.1

Sequence conflict

303 – 309

7

SSQAPPP → PHRPHLR in CAA60014. Ref.2

Sequence conflict

360

1

P → PV in CAA60014. Ref.2

Secondary structure

1

.

503

Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified March 1, 2005. Version 2. Checksum: 05FEF91E6D11B86D Show »	FASTA	503	51,275
10 20 30 40 50 60 MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV TNDRSAPILD 70 80 90 100 110 120 KPKGAGAGGG GGGFGGGGGF GGGGGGGGGG SFGGGGPPGL GGLFQAGMPK LRSTANRDND 130 140 150 160 170 180 SGGSRPPLLP PGGRSTSAKP FSPPSGPGRF PVPSPGHRSG PPEPQRNRMP PPRPDVGSKP 190 200 210 220 230 240 DSIPPPVPST PRPIQSSLHN RGSPPVPGGP RQPSPGPTPP PFPGNRGTAL GGGSIRQSPL 250 260 270 280 290 300 SSSSPFSNRP PLPPTPSRAL DDKPPPPPPP VGNRPSIHRE AVPPPPPQNN KPPVPSTPRP 310 320 330 340 350 360 SASSQAPPPP PPPSRPGPPP LPPSSSGNDE TPRLPQRNLS LSSSTPPLPS PGRSGPLPPP 370 380 390 400 410 420 PSERPPPPVR DPPGRSGPLP PPPPVSRNGS TSRALPATPQ LPSRSGVDSP RSGPRPPLPP 430 440 450 460 470 480 DRPSAGAPPP PPPSTSIRNG FQDSPCEDEW ESRFYFHPIS DLPPPEPYVQ TTKSYPSKLA 490 500 RNESRSGSNR RERGAPPLPP IPR « Hide
Isoform 2 [UniParc]. Checksum: E1D0FD646D9F0FB0 Show »		493	50,297
10 20 30 40 50 60 MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV TNDRSAPILD 70 80 90 100 110 120 KPKGAGAGGG GGGFGGGGGF GGGGGGGGGG SFGGGGPPGL GGLFQAGMPK LRSTANRDND 130 140 150 160 170 180 SGGSRPPLLP PGGRSTSAKP FSPPSGPGRF PVPSPGHRSG PPEPQRNRMP PPRPDVGSKP 190 200 210 220 230 240 DSIPPPVPST PRPIQSSLHN RGSPPVPGGP RQPSPGPTPP PFPGNRGTAL GGGSIRQSPL 250 260 270 280 290 300 SSSSPFSNRP PLPPTPSRAL DDKPPPPPPP VGNRPSIHRE AVPPPPPQNN KPPVPSTPRP 310 320 330 340 350 360 SASSQAPPPP PPPSRPGPPP LPPSSSGNDE TPRLPQRNLS LSSSTPPLPS PGRSGPLPPP 370 380 390 400 410 420 PSERPPPPVR DPPGRSGPLP PPPPVSRNGS TSRALPATPQ LPSRSGVDSP RSGPRPPLPP 430 440 450 460 470 480 DRPSAGAPPP PPPSTSIRNG FQDSPCEDEW ESRFYFHPIS DLPPPEPYVQ TTKSYPSKLA 490 RNESRSEYFC QGF « Hide
Isoform 3 [UniParc]. Checksum: FAA8FEB6396FA7F5 Show »		510	51,996
10 20 30 40 50 60 MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV TNDRSAPILD 70 80 90 100 110 120 KPKGAGAGGG GGGFGGGGGF GGGGGGGGGG SFGGGGPPGL GGLFQAGMPK LRSTANRDND 130 140 150 160 170 180 SGGSRPPLLP PGGRSTSAKP FSPPSGPGRF PVPSPGHRSG PPEPQRNRMP PPRPDVGSKP 190 200 210 220 230 240 DSIPPPVPST PRPIQSSLHN RGSPPVPGGP RQPSPGPTPP PFPGNRGTAL GGGSIRQSPL 250 260 270 280 290 300 SSSSPFSNRP PLPPTPSRAL DDKPPPPPPP VGNRPSIHRE AVPPPPPQNN KPPVPSTPRP 310 320 330 340 350 360 SASSQAPPPP PPPSRPGPPP LPPSSSGNDE TPRLPQRNLS LSSSTPPLPS PGRSGPLPPP 370 380 390 400 410 420 PSERPPPPVR DPPGRSGPLP PPPPVSRNGS TSRALPATPQ LPSRSGVDSP RSGPRPPLPP 430 440 450 460 470 480 DRPSAGAPPP PPPSTSIRNG FQDSPCEDEW ESRFYFHPIS DLPPPEPYVQ TTKSYPSKLA 490 500 510 RNESRSGSNR RERGAPPLPP IPRPPKQAAE « Hide
Isoform 4 [UniParc]. Checksum: 124197B8BB8321F1 Show »		358	36,442
10 20 30 40 50 60 MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV TNDRSAPILD 70 80 90 100 110 120 KPKGAGAGGG GGGFGGGGGF GGGGGGGGGG SFGGGGPPGL GGLFQAGMPK LRSTANRDND 130 140 150 160 170 180 SGGSRPPLLP PGGRSTSAKP FSPPSGPGRF PVPSPGHRSG PPEPQRNRMP PPRPDVGSKP 190 200 210 220 230 240 DSIPPPVPST PRPIQSSLHN RGSPPVPGGP RQPSPGPTPP PPPVRDPPGR SGPLPPPPPV 250 260 270 280 290 300 SRNGSTSRAL PATPQLPSRS GVDSPRSGPR PPLPPDRPSA GAPPPPPPST SIRNGFQDSP 310 320 330 340 350 CEDEWESRFY FHPISDLPPP EPYVQTTKSY PSKLARNESR SGSNRRERGA PPLPPIPR « Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"WIP, a protein associated with Wiskott-Aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cells." Ramesh N., Anton I.M., Hartwig J.H., Geha R.S. Proc. Natl. Acad. Sci. U.S.A. 94:14671-14676(1997) [PubMed: 9405671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLY-495, FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH WASP; PROFILIN AND ACTIN.
[2]	Kreideweiss S., Delany-Heiken P., Nordheim A., Ruhlmann A. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT GLY-495. Tissue: Tonsil.
[3]	The German cDNA consortium Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Endometrial tumor.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PRO-198.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Skin.
[6]	"Mutations that cause the Wiskott-Aldrich syndrome impair the interaction of Wiskott-Aldrich syndrome protein (WASP) with WASP interacting protein." Stewart D.M., Tian L., Nelson D.L. J. Immunol. 162:5019-5024(1999) [PubMed: 10202051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 192-503 (ISOFORM 1), INTERACTION WITH WASP.
[7]	"A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization." Moreau V., Frischknecht F., Reckmann I., Vincentelli R., Rabut G., Stewart D.M., Way M. Nat. Cell Biol. 2:441-448(2000) [PubMed: 10878810] [Abstract] Cited for: FUNCTION.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

AF031588 mRNA. Translation: AAC03767.1.
X86019 mRNA. Translation: CAA60014.1.
BX640870 mRNA. Translation: CAE45928.1.
AC010894 Genomic DNA. Translation: AAY14708.1.
BC002914 mRNA. Translation: AAH02914.1.
AF106062 mRNA. Translation: AAD45972.1.

RefSeq

NP_001070737.1.
NP_003378.3.

UniGene

Hs.654521

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MKE	NMR	-	A	-	[»]
2A41	X-ray	2.60	C	29-60	[»]

SMR

O43516. Positions 451-495.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:17015N.

IntAct

O43516.

PTM databases

PhosphoSite

O43516.

Genome annotation databases

Ensembl

ENSG00000115935. Homo sapiens. [Contig view]

GeneID

7456.

KEGG

hsa:7456.

Organism-specific databases

H-InvDB

HIX0034792.

HGNC

HGNC:12736. WIPF1.

HPA

CAB009737.

MIM

602357. gene.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOVERGEN

O43516.

Gene expression databases

ArrayExpress

O43516.

CleanEx

HS_WIPF1.

GermOnline

ENSG00000115935. Homo sapiens.

Family and domain databases

InterPro

IPR003124. WH2_actin_bd.
[Graphical view]

Pfam

PF02205. WH2. 1 hit.
[Graphical view]

SMART

SM00246. WH2. 1 hit.
[Graphical view]

PROSITE

PS51082. WH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

29198.

SOURCE

Search...

Hide | Top

Entry information

Entry name

WIPF1_HUMAN

Accession

Primary (citable) accession number: O43516
Secondary accession number(s): Q15220

Q9UNP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	March 1, 2005
Last modified:	November 25, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents