UniProtKB - O43516 (WIPF1_HUMAN)
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Protein
WAS/WASL-interacting protein family member 1
Gene
WIPF1
Organism
Homo sapiens (Human)
Status
Functioni
Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.By similarity3 Publications
Miscellaneous
Recruited to PIP5K-induced vesicle surfaces in the absence of functional WASL.By similarity
Caution
GO - Molecular functioni
- actin binding Source: ProtInc
- profilin binding Source: ProtInc
- SH3 domain binding Source: UniProtKB
GO - Biological processi
- actin filament-based movement Source: Ensembl
- actin polymerization or depolymerization Source: ProtInc
- Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
- protein-containing complex assembly Source: ProtInc
- response to other organism Source: Ensembl
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
Reactomei | R-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs |
SIGNORi | O43516 |
Names & Taxonomyi
Protein namesi | Recommended name: WAS/WASL-interacting protein family member 1Alternative name(s): Protein PRPL-2 Wiskott-Aldrich syndrome protein-interacting protein Short name: WASP-interacting protein |
Gene namesi | Name:WIPF1 Synonyms:WASPIP, WIP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000115935.16 |
HGNCi | HGNC:12736 WIPF1 |
MIMi | 602357 gene |
neXtProti | NX_O43516 |
Subcellular locationi
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoplasmic vesicle, CytoskeletonPathology & Biotechi
Involvement in diseasei
Wiskott-Aldrich syndrome 2 (WAS2)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn immunodeficiency disorder characterized by eczema, thrombocytopenia, recurrent infections, defective T-cell proliferation, and impaired natural killer cell function.
See also OMIM:614493Organism-specific databases
DisGeNETi | 7456 |
MalaCardsi | WIPF1 |
MIMi | 614493 phenotype |
OpenTargetsi | ENSG00000115935 |
Orphaneti | 906 Wiskott-Aldrich syndrome |
PharmGKBi | PA162409189 |
Polymorphism and mutation databases
BioMutai | WIPF1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000065941 | 1 – 503 | WAS/WASL-interacting protein family member 1Add BLAST | 503 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 33 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 125 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 134 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 142 | PhosphoserineBy similarity | 1 | |
Modified residuei | 234 | PhosphoserineCombined sources | 1 | |
Modified residuei | 340 | PhosphoserineCombined sources | 1 | |
Modified residuei | 345 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 350 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
MaxQBi | O43516 |
PaxDbi | O43516 |
PeptideAtlasi | O43516 |
PRIDEi | O43516 |
PTM databases
iPTMneti | O43516 |
PhosphoSitePlusi | O43516 |
Expressioni
Tissue specificityi
Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.1 Publication
Gene expression databases
Bgeei | ENSG00000115935 |
CleanExi | HS_WIPF1 |
ExpressionAtlasi | O43516 baseline and differential |
Genevisiblei | O43516 HS |
Organism-specific databases
HPAi | CAB009737 HPA003739 HPA070706 |
Interactioni
Subunit structurei
Binds to WAS, profilin and actin. Binds to WASL (By similarity). Interacts with DBNL. Interacts with FNBP1L (via the SH3 domain) (PubMed:19798448).By similarity5 Publications
Binary interactionsi
GO - Molecular functioni
- actin binding Source: ProtInc
- profilin binding Source: ProtInc
- SH3 domain binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 11329529 interactors. |
CORUMi | O43516 |
DIPi | DIP-17015N |
ELMi | O43516 |
IntActi | O43516 29 interactors. |
MINTi | O43516 |
STRINGi | 9606.ENSP00000352802 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 33 – 42 | Combined sources | 10 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2A41 | X-ray | 2.60 | C | 29-60 | [»] | |
ProteinModelPortali | O43516 | |||||
SMRi | O43516 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O43516 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 49 | WH2PROSITE-ProRule annotationAdd BLAST | 18 | |
Repeati | 352 – 361 | XRSGPXPPXP motif 1 | 10 | |
Repeati | 374 – 383 | XRSGPXPPXP motif 2 | 10 | |
Repeati | 410 – 419 | XRSGPXPPXP motif 3 | 10 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 45 – 48 | Binds actin | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 2 – 13 | Poly-ProAdd BLAST | 12 | |
Compositional biasi | 64 – 96 | Gly-richAdd BLAST | 33 | |
Compositional biasi | 241 – 244 | Poly-Ser | 4 | |
Compositional biasi | 264 – 433 | Pro-richAdd BLAST | 170 |
Domaini
Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.
Sequence similaritiesi
Belongs to the verprolin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4462 Eukaryota ENOG4111FVT LUCA |
GeneTreei | ENSGT00910000144296 |
HOVERGENi | HBG054157 |
InParanoidi | O43516 |
KOi | K19475 |
OMAi | QSSLHNR |
OrthoDBi | EOG091G16MU |
PhylomeDBi | O43516 |
TreeFami | TF332135 |
Family and domain databases
Gene3Di | 2.30.29.301 hit |
InterProi | View protein in InterPro IPR011993 PH-like_dom_sf IPR003124 WH2_dom IPR028295 WIP |
PANTHERi | PTHR23202:SF32 PTHR23202:SF32, 1 hit |
Pfami | View protein in Pfam PF02205 WH2, 1 hit |
SMARTi | View protein in SMART SM00246 WH2, 1 hit |
PROSITEi | View protein in PROSITE PS51082 WH2, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: O43516-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV
60 70 80 90 100
TNDRSAPILD KPKGAGAGGG GGGFGGGGGF GGGGGGGGGG SFGGGGPPGL
110 120 130 140 150
GGLFQAGMPK LRSTANRDND SGGSRPPLLP PGGRSTSAKP FSPPSGPGRF
160 170 180 190 200
PVPSPGHRSG PPEPQRNRMP PPRPDVGSKP DSIPPPVPST PRPIQSSPHN
210 220 230 240 250
RGSPPVPGGP RQPSPGPTPP PFPGNRGTAL GGGSIRQSPL SSSSPFSNRP
260 270 280 290 300
PLPPTPSRAL DDKPPPPPPP VGNRPSIHRE AVPPPPPQNN KPPVPSTPRP
310 320 330 340 350
SASSQAPPPP PPPSRPGPPP LPPSSSGNDE TPRLPQRNLS LSSSTPPLPS
360 370 380 390 400
PGRSGPLPPP PSERPPPPVR DPPGRSGPLP PPPPVSRNGS TSRALPATPQ
410 420 430 440 450
LPSRSGVDSP RSGPRPPLPP DRPSAGAPPP PPPSTSIRNG FQDSPCEDEW
460 470 480 490 500
ESRFYFHPIS DLPPPEPYVQ TTKSYPSKLA RNESRSGSNR RERGAPPLPP
IPR
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 119 | N → D in CAE45928 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 120 | D → H in AAH02914 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 360 | P → PV in CAA60014 (Ref. 2) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_046526 | 198 | P → L6 PublicationsCorresponds to variant dbSNP:rs4972450Ensembl. | 1 | |
Natural variantiVAR_010295 | 495 | A → G2 Publications | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012964 | 220 – 364 | Missing in isoform 4. 1 PublicationAdd BLAST | 145 | |
Alternative sequenceiVSP_012965 | 487 – 503 | GSNRR…PPIPR → EYFCQGF in isoform 2. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_012966 | 503 | R → RPPKQAAE in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF031588 mRNA Translation: AAC03767.1 Frameshift. X86019 mRNA Translation: CAA60014.1 Frameshift. BX640870 mRNA Translation: CAE45928.1 AC010894 Genomic DNA Translation: AAY14708.1 AC104595 Genomic DNA No translation available. CH471058 Genomic DNA Translation: EAX11131.1 CH471058 Genomic DNA Translation: EAX11132.1 CH471058 Genomic DNA Translation: EAX11133.1 BC002914 mRNA Translation: AAH02914.1 AF106062 mRNA Translation: AAD45972.1 |
CCDSi | CCDS2260.1 [O43516-1] |
RefSeqi | NP_001070737.1, NM_001077269.1 [O43516-1] NP_003378.3, NM_003387.4 [O43516-1] XP_011510082.1, XM_011511780.2 [O43516-1] |
UniGenei | Hs.128067 |
Genome annotation databases
Ensembli | ENST00000272746; ENSP00000272746; ENSG00000115935 [O43516-3] ENST00000359761; ENSP00000352802; ENSG00000115935 [O43516-1] ENST00000392546; ENSP00000376329; ENSG00000115935 [O43516-1] ENST00000392547; ENSP00000376330; ENSG00000115935 [O43516-1] ENST00000409891; ENSP00000386431; ENSG00000115935 [O43516-2] |
GeneIDi | 7456 |
KEGGi | hsa:7456 |
UCSCi | uc002uiz.4 human [O43516-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Entry informationi
Entry namei | WIPF1_HUMAN | |
Accessioni | O43516Primary (citable) accession number: O43516 Secondary accession number(s): B8ZZM1 Q9UNP1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 30, 2010 | |
Last modified: | April 25, 2018 | |
This is version 169 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |