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Protein

WAS/WASL-interacting protein family member 1

Gene

WIPF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.By similarity3 Publications

Miscellaneous

Recruited to PIP5K-induced vesicle surfaces in the absence of functional WASL.By similarity

GO - Molecular functioni

  • actin binding Source: ProtInc
  • profilin binding Source: ProtInc
  • SH3 domain binding Source: UniProtKB

GO - Biological processi

  • actin filament-based movement Source: Ensembl
  • actin polymerization or depolymerization Source: ProtInc
  • Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
  • protein complex assembly Source: ProtInc
  • response to other organism Source: Ensembl

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.
SIGNORiO43516.

Names & Taxonomyi

Protein namesi
Recommended name:
WAS/WASL-interacting protein family member 1
Alternative name(s):
Protein PRPL-2
Wiskott-Aldrich syndrome protein-interacting protein
Short name:
WASP-interacting protein
Gene namesi
Name:WIPF1
Synonyms:WASPIP, WIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115935.16.
HGNCiHGNC:12736. WIPF1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

Pathology & Biotechi

Involvement in diseasei

Wiskott-Aldrich syndrome 2 (WAS2)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn immunodeficiency disorder characterized by eczema, thrombocytopenia, recurrent infections, defective T-cell proliferation, and impaired natural killer cell function.
See also OMIM:614493

Organism-specific databases

DisGeNETi7456.
MalaCardsiWIPF1.
MIMi614493. phenotype.
OpenTargetsiENSG00000115935.
Orphaneti906. Wiskott-Aldrich syndrome.
PharmGKBiPA162409189.

Polymorphism and mutation databases

BioMutaiWIPF1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659411 – 503WAS/WASL-interacting protein family member 1Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33Asymmetric dimethylarginineBy similarity1
Modified residuei125Omega-N-methylarginineBy similarity1
Modified residuei134Omega-N-methylarginineBy similarity1
Modified residuei142PhosphoserineBy similarity1
Modified residuei234PhosphoserineCombined sources1
Modified residuei340PhosphoserineCombined sources1
Modified residuei345PhosphothreonineCombined sources1
Modified residuei350PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiO43516.
PaxDbiO43516.
PeptideAtlasiO43516.
PRIDEiO43516.

PTM databases

iPTMnetiO43516.
PhosphoSitePlusiO43516.

Expressioni

Tissue specificityi

Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.1 Publication

Gene expression databases

BgeeiENSG00000115935.
CleanExiHS_WIPF1.
ExpressionAtlasiO43516. baseline and differential.
GenevisibleiO43516. HS.

Organism-specific databases

HPAiCAB009737.
HPA003739.
HPA070706.

Interactioni

Subunit structurei

Binds to WAS, profilin and actin. Binds to WASL (By similarity). Interacts with DBNL. Interacts with FNBP1L (via the SH3 domain) (PubMed:19798448).By similarity5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin binding Source: ProtInc
  • profilin binding Source: ProtInc
  • SH3 domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi113295. 29 interactors.
CORUMiO43516.
DIPiDIP-17015N.
ELMiO43516.
IntActiO43516. 29 interactors.
MINTiMINT-238480.
STRINGi9606.ENSP00000352802.

Structurei

Secondary structure

1503
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 42Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A41X-ray2.60C29-60[»]
ProteinModelPortaliO43516.
SMRiO43516.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43516.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 49WH2PROSITE-ProRule annotationAdd BLAST18
Repeati352 – 361XRSGPXPPXP motif 110
Repeati374 – 383XRSGPXPPXP motif 210
Repeati410 – 419XRSGPXPPXP motif 310

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 48Binds actin4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 13Poly-ProAdd BLAST12
Compositional biasi64 – 96Gly-richAdd BLAST33
Compositional biasi241 – 244Poly-Ser4
Compositional biasi264 – 433Pro-richAdd BLAST170

Domaini

Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.

Sequence similaritiesi

Belongs to the verprolin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4462. Eukaryota.
ENOG4111FVT. LUCA.
GeneTreeiENSGT00810000125434.
HOVERGENiHBG054157.
InParanoidiO43516.
KOiK19475.
OMAiQSSLHNR.
OrthoDBiEOG091G16MU.
PhylomeDBiO43516.
TreeFamiTF332135.

Family and domain databases

InterProiView protein in InterPro
IPR003124. WH2_dom.
IPR028295. WIP.
PANTHERiPTHR23202:SF46. PTHR23202:SF46. 1 hit.
PfamiView protein in Pfam
PF02205. WH2. 1 hit.
SMARTiView protein in SMART
SM00246. WH2. 1 hit.
PROSITEiView protein in PROSITE
PS51082. WH2. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43516-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVPPPPAPP PPPTFALANT EKPTLNKTEQ AGRNALLSDI SKGKKLKKTV
60 70 80 90 100
TNDRSAPILD KPKGAGAGGG GGGFGGGGGF GGGGGGGGGG SFGGGGPPGL
110 120 130 140 150
GGLFQAGMPK LRSTANRDND SGGSRPPLLP PGGRSTSAKP FSPPSGPGRF
160 170 180 190 200
PVPSPGHRSG PPEPQRNRMP PPRPDVGSKP DSIPPPVPST PRPIQSSPHN
210 220 230 240 250
RGSPPVPGGP RQPSPGPTPP PFPGNRGTAL GGGSIRQSPL SSSSPFSNRP
260 270 280 290 300
PLPPTPSRAL DDKPPPPPPP VGNRPSIHRE AVPPPPPQNN KPPVPSTPRP
310 320 330 340 350
SASSQAPPPP PPPSRPGPPP LPPSSSGNDE TPRLPQRNLS LSSSTPPLPS
360 370 380 390 400
PGRSGPLPPP PSERPPPPVR DPPGRSGPLP PPPPVSRNGS TSRALPATPQ
410 420 430 440 450
LPSRSGVDSP RSGPRPPLPP DRPSAGAPPP PPPSTSIRNG FQDSPCEDEW
460 470 480 490 500
ESRFYFHPIS DLPPPEPYVQ TTKSYPSKLA RNESRSGSNR RERGAPPLPP

IPR
Length:503
Mass (Da):51,258
Last modified:November 30, 2010 - v3
Checksum:i09B7A95E6808A46D
GO
Isoform 2 (identifier: O43516-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     487-503: GSNRRERGAPPLPPIPR → EYFCQGF

Show »
Length:493
Mass (Da):50,281
Checksum:iE6BD4D77F6F2B6F4
GO
Isoform 3 (identifier: O43516-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     503-503: R → RPPKQAAE

Note: No experimental confirmation available.
Show »
Length:510
Mass (Da):51,980
Checksum:i9AEF92B15E4FE7F9
GO
Isoform 4 (identifier: O43516-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     220-364: Missing.

Note: No experimental confirmation available.
Show »
Length:358
Mass (Da):36,426
Checksum:i0473FF11AFC330B4
GO

Sequence cautioni

The sequence AAC03767 differs from that shown. Reason: Frameshift at positions 302 and 310.Curated
The sequence CAA60014 differs from that shown. Reason: Frameshift at positions 302 and 310.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119N → D in CAE45928 (PubMed:17974005).Curated1
Sequence conflicti120D → H in AAH02914 (PubMed:15489334).Curated1
Sequence conflicti360P → PV in CAA60014 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_046526198P → L6 PublicationsCorresponds to variant dbSNP:rs4972450Ensembl.1
Natural variantiVAR_010295495A → G2 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012964220 – 364Missing in isoform 4. 1 PublicationAdd BLAST145
Alternative sequenceiVSP_012965487 – 503GSNRR…PPIPR → EYFCQGF in isoform 2. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_012966503R → RPPKQAAE in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031588 mRNA. Translation: AAC03767.1. Frameshift.
X86019 mRNA. Translation: CAA60014.1. Frameshift.
BX640870 mRNA. Translation: CAE45928.1.
AC010894 Genomic DNA. Translation: AAY14708.1.
AC104595 Genomic DNA. No translation available.
CH471058 Genomic DNA. Translation: EAX11131.1.
CH471058 Genomic DNA. Translation: EAX11132.1.
CH471058 Genomic DNA. Translation: EAX11133.1.
BC002914 mRNA. Translation: AAH02914.1.
AF106062 mRNA. Translation: AAD45972.1.
CCDSiCCDS2260.1. [O43516-1]
RefSeqiNP_001070737.1. NM_001077269.1. [O43516-1]
NP_003378.3. NM_003387.4. [O43516-1]
XP_011510082.1. XM_011511780.2. [O43516-1]
UniGeneiHs.128067.

Genome annotation databases

EnsembliENST00000272746; ENSP00000272746; ENSG00000115935. [O43516-3]
ENST00000359761; ENSP00000352802; ENSG00000115935. [O43516-1]
ENST00000392546; ENSP00000376329; ENSG00000115935. [O43516-1]
ENST00000392547; ENSP00000376330; ENSG00000115935. [O43516-1]
ENST00000409891; ENSP00000386431; ENSG00000115935. [O43516-2]
GeneIDi7456.
KEGGihsa:7456.
UCSCiuc002uiz.4. human. [O43516-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiWIPF1_HUMAN
AccessioniPrimary (citable) accession number: O43516
Secondary accession number(s): B8ZZM1
, D3DPE4, Q15220, Q53TA9, Q6MZU9, Q9BU37, Q9UNP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 30, 2010
Last modified: September 27, 2017
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families