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O43513

- MED7_HUMAN

UniProt

O43513 - MED7_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 7

Gene

MED7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. RNA polymerase II transcription cofactor activity Source: InterPro
    3. transcription coactivator activity Source: ProtInc

    GO - Biological processi

    1. gene expression Source: Reactome
    2. regulation of transcription from RNA polymerase II promoter Source: MGI
    3. stem cell maintenance Source: Ensembl
    4. transcription initiation from RNA polymerase II promoter Source: Reactome

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 7
    Short name:
    hMED7
    Alternative name(s):
    Activator-recruited cofactor 34 kDa component
    Short name:
    ARC34
    Cofactor required for Sp1 transcriptional activation subunit 9
    Short name:
    CRSP complex subunit 9
    Mediator complex subunit 7
    RNA polymerase transcriptional regulation mediator subunit 7 homolog
    Transcriptional coactivator CRSP33
    Gene namesi
    Name:MED7
    Synonyms:ARC34, CRSP9
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:2378. MED7.

    Subcellular locationi

    GO - Cellular componenti

    1. mediator complex Source: Ensembl
    2. mitochondrion Source: HPA
    3. nuclear membrane Source: HPA
    4. nucleoplasm Source: Reactome
    5. nucleus Source: HPA
    6. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162395669.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 233233Mediator of RNA polymerase II transcription subunit 7PRO_0000079408Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei194 – 1941Phosphoserine1 Publication

    Post-translational modificationi

    Constitutively sumoylated.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiO43513.
    PaxDbiO43513.
    PeptideAtlasiO43513.
    PRIDEiO43513.

    PTM databases

    PhosphoSiteiO43513.

    Expressioni

    Gene expression databases

    ArrayExpressiO43513.
    BgeeiO43513.
    CleanExiHS_MED7.
    GenevestigatoriO43513.

    Organism-specific databases

    HPAiHPA048562.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

    Protein-protein interaction databases

    BioGridi114833. 42 interactions.
    IntActiO43513. 9 interactions.
    MINTiMINT-1457297.
    STRINGi9606.ENSP00000286317.

    Structurei

    3D structure databases

    ProteinModelPortaliO43513.
    SMRiO43513. Positions 12-47, 73-170.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 7 family.Curated

    Phylogenomic databases

    eggNOGiNOG274591.
    HOGENOMiHOG000241102.
    HOVERGENiHBG002997.
    InParanoidiO43513.
    KOiK15148.
    OMAiLLNEFRP.
    OrthoDBiEOG74XS6M.
    PhylomeDBiO43513.
    TreeFamiTF314411.

    Family and domain databases

    InterProiIPR009244. Mediatior_Med7.
    [Graphical view]
    PfamiPF05983. Med7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O43513-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ    50
    CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINFL DLLDILIRSP 100
    GSIKREEKLE DLKLLFVHVH HLINEYRPHQ ARETLRVMME VQKRQRLETA 150
    ERFQKHLERV IEMIQNCLAS LPDDLPHSEA GMRVKTEPMD ADDSNNCTGQ 200
    NEHQRENSGH RRDQIIEKDA ALCVLIDEMN ERP 233
    Length:233
    Mass (Da):27,245
    Last modified:June 1, 1998 - v1
    Checksum:i9D95A2A8360B9C62
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031383 mRNA. Translation: AAC52115.1.
    AF104251 mRNA. Translation: AAD12720.1.
    BC005250 mRNA. No translation available.
    CCDSiCCDS4334.1.
    RefSeqiNP_001094286.1. NM_001100816.1.
    NP_004261.1. NM_004270.4.
    UniGeneiHs.279902.
    Hs.744244.

    Genome annotation databases

    EnsembliENST00000286317; ENSP00000286317; ENSG00000155868.
    ENST00000420343; ENSP00000401046; ENSG00000155868.
    GeneIDi9443.
    KEGGihsa:9443.
    UCSCiuc003lwm.4. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031383 mRNA. Translation: AAC52115.1 .
    AF104251 mRNA. Translation: AAD12720.1 .
    BC005250 mRNA. No translation available.
    CCDSi CCDS4334.1.
    RefSeqi NP_001094286.1. NM_001100816.1.
    NP_004261.1. NM_004270.4.
    UniGenei Hs.279902.
    Hs.744244.

    3D structure databases

    ProteinModelPortali O43513.
    SMRi O43513. Positions 12-47, 73-170.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114833. 42 interactions.
    IntActi O43513. 9 interactions.
    MINTi MINT-1457297.
    STRINGi 9606.ENSP00000286317.

    PTM databases

    PhosphoSitei O43513.

    Proteomic databases

    MaxQBi O43513.
    PaxDbi O43513.
    PeptideAtlasi O43513.
    PRIDEi O43513.

    Protocols and materials databases

    DNASUi 9443.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000286317 ; ENSP00000286317 ; ENSG00000155868 .
    ENST00000420343 ; ENSP00000401046 ; ENSG00000155868 .
    GeneIDi 9443.
    KEGGi hsa:9443.
    UCSCi uc003lwm.4. human.

    Organism-specific databases

    CTDi 9443.
    GeneCardsi GC05M156564.
    HGNCi HGNC:2378. MED7.
    HPAi HPA048562.
    MIMi 605045. gene.
    neXtProti NX_O43513.
    PharmGKBi PA162395669.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG274591.
    HOGENOMi HOG000241102.
    HOVERGENi HBG002997.
    InParanoidi O43513.
    KOi K15148.
    OMAi LLNEFRP.
    OrthoDBi EOG74XS6M.
    PhylomeDBi O43513.
    TreeFami TF314411.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    GeneWikii MED7.
    GenomeRNAii 9443.
    NextBioi 35362.
    PROi O43513.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O43513.
    Bgeei O43513.
    CleanExi HS_MED7.
    Genevestigatori O43513.

    Family and domain databases

    InterProi IPR009244. Mediatior_Med7.
    [Graphical view ]
    Pfami PF05983. Med7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways."
      Jiang Y.W., Veschambre P., Erdjument-Bromage H., Tempst P., Conaway J.W., Conaway R.C., Kornberg R.D.
      Proc. Natl. Acad. Sci. U.S.A. 95:8538-8543(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1."
      Ryu S., Zhou S., Ladurner A.G., Tjian R.
      Nature 397:446-450(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Urinary bladder.
    4. "Composite co-activator ARC mediates chromatin-directed transcriptional activation."
      Naeaer A.M., Beaurang P.A., Zhou S., Abraham S., Solomon W.B., Tjian R.
      Nature 398:828-832(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN ARC COMPLEX, PROTEIN SEQUENCE OF 19-29 AND 212-222.
    5. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    6. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    7. "Ubc9 fusion-directed SUMOylation identifies constitutive and inducible SUMOylation."
      Jakobs A., Himstedt F., Funk M., Korn B., Gaestel M., Niedenthal R.
      Nucleic Acids Res. 35:E109-E109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMED7_HUMAN
    AccessioniPrimary (citable) accession number: O43513
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 28, 2003
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3