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Protein

Mediator of RNA polymerase II transcription subunit 7

Gene

MED7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

  • RNA polymerase II transcription cofactor activity Source: InterPro
  • transcription coactivator activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 7
Short name:
hMED7
Alternative name(s):
Activator-recruited cofactor 34 kDa component
Short name:
ARC34
Cofactor required for Sp1 transcriptional activation subunit 9
Short name:
CRSP complex subunit 9
Mediator complex subunit 7
RNA polymerase transcriptional regulation mediator subunit 7 homolog
Transcriptional coactivator CRSP33
Gene namesi
Name:MED7
Synonyms:ARC34, CRSP9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:2378. MED7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395669.

Polymorphism and mutation databases

BioMutaiMED7.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Mediator of RNA polymerase II transcription subunit 7PRO_0000079408Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei194 – 1941Phosphoserine1 Publication

Post-translational modificationi

Constitutively sumoylated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO43513.
PaxDbiO43513.
PeptideAtlasiO43513.
PRIDEiO43513.

PTM databases

PhosphoSiteiO43513.

Expressioni

Gene expression databases

BgeeiO43513.
CleanExiHS_MED7.
ExpressionAtlasiO43513. baseline and differential.
GenevestigatoriO43513.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
envP033833EBI-394632,EBI-9676086From a different organism.
MED1Q156484EBI-394632,EBI-394459
MED10Q9BTT45EBI-394632,EBI-394354
MED26O954026EBI-394632,EBI-394392

Protein-protein interaction databases

BioGridi114833. 60 interactions.
IntActiO43513. 13 interactions.
MINTiMINT-1457297.
STRINGi9606.ENSP00000286317.

Structurei

3D structure databases

ProteinModelPortaliO43513.
SMRiO43513. Positions 106-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 7 family.Curated

Phylogenomic databases

eggNOGiNOG274591.
HOGENOMiHOG000241102.
HOVERGENiHBG002997.
InParanoidiO43513.
KOiK15148.
OMAiMCSIIDE.
OrthoDBiEOG74XS6M.
PhylomeDBiO43513.
TreeFamiTF314411.

Family and domain databases

InterProiIPR009244. Mediatior_Med7.
[Graphical view]
PfamiPF05983. Med7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43513-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ
60 70 80 90 100
CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINFL DLLDILIRSP
110 120 130 140 150
GSIKREEKLE DLKLLFVHVH HLINEYRPHQ ARETLRVMME VQKRQRLETA
160 170 180 190 200
ERFQKHLERV IEMIQNCLAS LPDDLPHSEA GMRVKTEPMD ADDSNNCTGQ
210 220 230
NEHQRENSGH RRDQIIEKDA ALCVLIDEMN ERP
Length:233
Mass (Da):27,245
Last modified:June 1, 1998 - v1
Checksum:i9D95A2A8360B9C62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031383 mRNA. Translation: AAC52115.1.
AF104251 mRNA. Translation: AAD12720.1.
BC005250 mRNA. No translation available.
CCDSiCCDS4334.1.
RefSeqiNP_001094286.1. NM_001100816.1.
NP_004261.1. NM_004270.4.
UniGeneiHs.279902.
Hs.744244.

Genome annotation databases

EnsembliENST00000286317; ENSP00000286317; ENSG00000155868.
ENST00000420343; ENSP00000401046; ENSG00000155868.
GeneIDi9443.
KEGGihsa:9443.
UCSCiuc003lwm.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031383 mRNA. Translation: AAC52115.1.
AF104251 mRNA. Translation: AAD12720.1.
BC005250 mRNA. No translation available.
CCDSiCCDS4334.1.
RefSeqiNP_001094286.1. NM_001100816.1.
NP_004261.1. NM_004270.4.
UniGeneiHs.279902.
Hs.744244.

3D structure databases

ProteinModelPortaliO43513.
SMRiO43513. Positions 106-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114833. 60 interactions.
IntActiO43513. 13 interactions.
MINTiMINT-1457297.
STRINGi9606.ENSP00000286317.

PTM databases

PhosphoSiteiO43513.

Polymorphism and mutation databases

BioMutaiMED7.

Proteomic databases

MaxQBiO43513.
PaxDbiO43513.
PeptideAtlasiO43513.
PRIDEiO43513.

Protocols and materials databases

DNASUi9443.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000286317; ENSP00000286317; ENSG00000155868.
ENST00000420343; ENSP00000401046; ENSG00000155868.
GeneIDi9443.
KEGGihsa:9443.
UCSCiuc003lwm.4. human.

Organism-specific databases

CTDi9443.
GeneCardsiGC05M156564.
HGNCiHGNC:2378. MED7.
MIMi605045. gene.
neXtProtiNX_O43513.
PharmGKBiPA162395669.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG274591.
HOGENOMiHOG000241102.
HOVERGENiHBG002997.
InParanoidiO43513.
KOiK15148.
OMAiMCSIIDE.
OrthoDBiEOG74XS6M.
PhylomeDBiO43513.
TreeFamiTF314411.

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

GeneWikiiMED7.
GenomeRNAii9443.
NextBioi35362.
PROiO43513.
SOURCEiSearch...

Gene expression databases

BgeeiO43513.
CleanExiHS_MED7.
ExpressionAtlasiO43513. baseline and differential.
GenevestigatoriO43513.

Family and domain databases

InterProiIPR009244. Mediatior_Med7.
[Graphical view]
PfamiPF05983. Med7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways."
    Jiang Y.W., Veschambre P., Erdjument-Bromage H., Tempst P., Conaway J.W., Conaway R.C., Kornberg R.D.
    Proc. Natl. Acad. Sci. U.S.A. 95:8538-8543(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1."
    Ryu S., Zhou S., Ladurner A.G., Tjian R.
    Nature 397:446-450(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Urinary bladder.
  4. "Composite co-activator ARC mediates chromatin-directed transcriptional activation."
    Naeaer A.M., Beaurang P.A., Zhou S., Abraham S., Solomon W.B., Tjian R.
    Nature 398:828-832(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN ARC COMPLEX, PROTEIN SEQUENCE OF 19-29 AND 212-222.
  5. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  6. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  7. "Ubc9 fusion-directed SUMOylation identifies constitutive and inducible SUMOylation."
    Jakobs A., Himstedt F., Funk M., Korn B., Gaestel M., Niedenthal R.
    Nucleic Acids Res. 35:E109-E109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMED7_HUMAN
AccessioniPrimary (citable) accession number: O43513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: June 1, 1998
Last modified: April 29, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.