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Protein

Disintegrin and metalloproteinase domain-containing protein 20

Gene

ADAM20

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in sperm maturation and/or fertilization.

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi173Zinc; in inhibited formBy similarity1
Metal bindingi342Zinc; catalyticSequence analysis1
Active sitei343PROSITE-ProRule annotation1
Metal bindingi346Zinc; catalyticSequence analysis1
Metal bindingi352Zinc; catalyticSequence analysis1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: ProtInc

GO - Biological processi

  • binding of sperm to zona pellucida Source: Reactome
  • single fertilization Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000134007-MONOMER.
ReactomeiR-HSA-1300644. Interaction With The Zona Pellucida.

Protein family/group databases

MEROPSiM12.218.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 20 (EC:3.4.24.-)
Short name:
ADAM 20
Gene namesi
Name:ADAM20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:199. ADAM20.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini207 – 693ExtracellularSequence analysisAdd BLAST487
Transmembranei694 – 714HelicalSequence analysisAdd BLAST21
Topological domaini715 – 726CytoplasmicSequence analysisAdd BLAST12

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8748.
PharmGKBiPA24516.

Polymorphism and mutation databases

BioMutaiADAM20.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
PropeptideiPRO_000002910632 – 206Sequence analysisAdd BLAST175
ChainiPRO_0000029107207 – 726Disintegrin and metalloproteinase domain-containing protein 20Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi191N-linked (GlcNAc...)Sequence analysis1
Glycosylationi226N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi317 ↔ 394By similarity
Disulfide bondi357 ↔ 379By similarity
Disulfide bondi359 ↔ 364By similarity
Glycosylationi378N-linked (GlcNAc...)Sequence analysis1
Glycosylationi438N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi465 ↔ 485By similarity
Glycosylationi479N-linked (GlcNAc...)Sequence analysis1
Glycosylationi587N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi635 ↔ 646By similarity
Disulfide bondi640 ↔ 652By similarity
Disulfide bondi654 ↔ 663By similarity

Post-translational modificationi

Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO43506.
PRIDEiO43506.

PTM databases

iPTMnetiO43506.
PhosphoSitePlusiO43506.

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

BgeeiENSG00000134007.
CleanExiHS_ADAM20.
GenevisibleiO43506. HS.

Organism-specific databases

HPAiHPA059377.

Interactioni

Protein-protein interaction databases

BioGridi114284. 1 interactor.
IntActiO43506. 1 interactor.
MINTiMINT-7970672.
STRINGi9606.ENSP00000256389.

Structurei

3D structure databases

ProteinModelPortaliO43506.
SMRiO43506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 400Peptidase M12BPROSITE-ProRule annotationAdd BLAST194
Domaini407 – 493DisintegrinPROSITE-ProRule annotationAdd BLAST87
Domaini635 – 663EGF-likeAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi171 – 178Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi494 – 634Cys-richAdd BLAST141

Domaini

A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiO43506.
KOiK08609.
OrthoDBiEOG091G03BZ.
PhylomeDBiO43506.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR033612. ADAM20.
IPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PANTHERiPTHR11905:SF135. PTHR11905:SF135. 1 hit.
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVGEPLVHI RVTLLLLWFG MFLSISGHSQ ARPSQYFTSP EVVIPLKVIS
60 70 80 90 100
RGRGAKAPGW LSYSLRFGGQ RYIVHMRVNK LLFAAHLPVF TYTEQHALLQ
110 120 130 140 150
DQPFIQDDCY YHGYVEGVPE SLVALSTCSG GFLGMLQIND LVYEIKPISV
160 170 180 190 200
SATFEHLVYK IDSDDTQFPP MRCGLTEEKI AHQMELQLSY NFTLKQSSFV
210 220 230 240 250
GWWTHQRFVE LVVVVDNIRY LFSQSNATTV QHEVFNVVNI VDSFYHPLEV
260 270 280 290 300
DVILTGIDIW TASNPLPTSG DLDNVLEDFS IWKNYNLNNR LQHDVAHLFI
310 320 330 340 350
KDTQGMKLGV AYVKGICQNP FNTGVDVFED NRLVVFAITL GHELGHNLGM
360 370 380 390 400
QHDTQWCVCE LQWCIMHAYR KVTTKFSNCS YAQYWDSTIS SGLCIQPPPY
410 420 430 440 450
PGNIFRLKYC GNLVVEEGEE CDCGTIRQCA KDPCCLLNCT LHPGAACAFG
460 470 480 490 500
ICCKDCKFLP SGTLCRQQVG ECDLPEWCNG TSHQCPDDVY VQDGISCNVN
510 520 530 540 550
AFCYEKTCNN HDIQCKEIFG QDARSASQSC YQEINTQGNR FGHCGIVGTT
560 570 580 590 600
YVKCWTPDIM CGRVQCENVG VIPNLIEHST VQQFHLNDTT CWGTDYHLGM
610 620 630 640 650
AIPDIGEVKD GTVCGPEKIC IRKKCASMVH LSQACQPKTC NMRGICNNKQ
660 670 680 690 700
HCHCNHEWAP PYCKDKGYGG SADSGPPPKN NMEGLNVMGK LRYLSLLCLL
710 720
PLVAFLLFCL HVLFKKRTKS KEDEEG
Length:726
Mass (Da):81,603
Last modified:November 25, 2008 - v2
Checksum:i7DB864FA1796A9B0
GO

Sequence cautioni

The sequence AAH25378 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH25378 differs from that shown. Reason: Erroneous initiation.Curated
The sequence EAW81037 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109C → W in AAC52041 (PubMed:9469942).Curated1
Sequence conflicti637P → R in AAC52041 (PubMed:9469942).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04731119F → L.1 PublicationCorresponds to variant rs1059166dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029899 mRNA. Translation: AAC52041.1.
AF158643 Genomic DNA. Translation: AAD55254.1.
AL357153 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81037.1. Different initiation.
BC025378 mRNA. Translation: AAH25378.2. Different initiation.
RefSeqiNP_003805.3. NM_003814.4.
XP_005268208.1. XM_005268151.3.
UniGeneiHs.177984.

Genome annotation databases

EnsembliENST00000256389; ENSP00000256389; ENSG00000134007.
GeneIDi8748.
KEGGihsa:8748.
UCSCiuc001xme.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029899 mRNA. Translation: AAC52041.1.
AF158643 Genomic DNA. Translation: AAD55254.1.
AL357153 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81037.1. Different initiation.
BC025378 mRNA. Translation: AAH25378.2. Different initiation.
RefSeqiNP_003805.3. NM_003814.4.
XP_005268208.1. XM_005268151.3.
UniGeneiHs.177984.

3D structure databases

ProteinModelPortaliO43506.
SMRiO43506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114284. 1 interactor.
IntActiO43506. 1 interactor.
MINTiMINT-7970672.
STRINGi9606.ENSP00000256389.

Protein family/group databases

MEROPSiM12.218.

PTM databases

iPTMnetiO43506.
PhosphoSitePlusiO43506.

Polymorphism and mutation databases

BioMutaiADAM20.

Proteomic databases

PaxDbiO43506.
PRIDEiO43506.

Protocols and materials databases

DNASUi8748.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000256389; ENSP00000256389; ENSG00000134007.
GeneIDi8748.
KEGGihsa:8748.
UCSCiuc001xme.4. human.

Organism-specific databases

CTDi8748.
DisGeNETi8748.
GeneCardsiADAM20.
HGNCiHGNC:199. ADAM20.
HPAiHPA059377.
MIMi603712. gene.
neXtProtiNX_O43506.
PharmGKBiPA24516.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiO43506.
KOiK08609.
OrthoDBiEOG091G03BZ.
PhylomeDBiO43506.
TreeFamiTF314733.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000134007-MONOMER.
ReactomeiR-HSA-1300644. Interaction With The Zona Pellucida.

Miscellaneous databases

GenomeRNAii8748.
PROiO43506.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134007.
CleanExiHS_ADAM20.
GenevisibleiO43506. HS.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR033612. ADAM20.
IPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PANTHERiPTHR11905:SF135. PTHR11905:SF135. 1 hit.
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADA20_HUMAN
AccessioniPrimary (citable) accession number: O43506
Secondary accession number(s): Q6GTZ1, Q9UKJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 25, 2008
Last modified: November 2, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.