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O43506

- ADA20_HUMAN

UniProt

O43506 - ADA20_HUMAN

Protein

Disintegrin and metalloproteinase domain-containing protein 20

Gene

ADAM20

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    May be involved in sperm maturation and/or fertilization.

    Cofactori

    Binds 1 zinc ion per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi173 – 1731Zinc; in inhibited formBy similarity
    Metal bindingi342 – 3421Zinc; catalyticSequence Analysis
    Active sitei343 – 3431PROSITE-ProRule annotation
    Metal bindingi346 – 3461Zinc; catalyticSequence Analysis
    Metal bindingi352 – 3521Zinc; catalyticSequence Analysis

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: InterPro
    2. metallopeptidase activity Source: ProtInc
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. binding of sperm to zona pellucida Source: Reactome
    2. multicellular organism reproduction Source: Reactome
    3. single fertilization Source: Reactome

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_163933. Interaction With The Zona Pellucida.

    Protein family/group databases

    MEROPSiM12.218.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 20 (EC:3.4.24.-)
    Short name:
    ADAM 20
    Gene namesi
    Name:ADAM20
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:199. ADAM20.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24516.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Propeptidei32 – 206175Sequence AnalysisPRO_0000029106Add
    BLAST
    Chaini207 – 726520Disintegrin and metalloproteinase domain-containing protein 20PRO_0000029107Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi317 ↔ 394By similarity
    Disulfide bondi357 ↔ 379By similarity
    Disulfide bondi359 ↔ 364By similarity
    Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi438 – 4381N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi465 ↔ 485By similarity
    Glycosylationi479 – 4791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi587 – 5871N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi635 ↔ 646By similarity
    Disulfide bondi640 ↔ 652By similarity
    Disulfide bondi654 ↔ 663By similarity

    Post-translational modificationi

    Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiO43506.
    PRIDEiO43506.

    PTM databases

    PhosphoSiteiO43506.

    Expressioni

    Tissue specificityi

    Testis specific.

    Gene expression databases

    BgeeiO43506.
    CleanExiHS_ADAM20.
    GenevestigatoriO43506.

    Interactioni

    Protein-protein interaction databases

    BioGridi114284. 1 interaction.
    IntActiO43506. 1 interaction.
    MINTiMINT-7970672.
    STRINGi9606.ENSP00000256389.

    Structurei

    3D structure databases

    ProteinModelPortaliO43506.
    SMRiO43506. Positions 206-628.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini207 – 693487ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini715 – 72612CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei694 – 71421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini207 – 400194Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini407 – 49387DisintegrinPROSITE-ProRule annotationAdd
    BLAST
    Domaini635 – 66329EGF-likeAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi171 – 1788Cysteine switchBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi494 – 634141Cys-richAdd
    BLAST

    Domaini

    A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
    The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    Sequence similaritiesi

    Contains 1 disintegrin domain.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.Curated
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG246189.
    HOGENOMiHOG000230883.
    HOVERGENiHBG006978.
    InParanoidiO43506.
    KOiK08609.
    OrthoDBiEOG7KWSGW.
    PhylomeDBiO43506.
    TreeFamiTF314733.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProiIPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view]
    PfamiPF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view]
    PRINTSiPR00289. DISINTEGRIN.
    SMARTiSM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF57552. SSF57552. 1 hit.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O43506-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVGEPLVHI RVTLLLLWFG MFLSISGHSQ ARPSQYFTSP EVVIPLKVIS    50
    RGRGAKAPGW LSYSLRFGGQ RYIVHMRVNK LLFAAHLPVF TYTEQHALLQ 100
    DQPFIQDDCY YHGYVEGVPE SLVALSTCSG GFLGMLQIND LVYEIKPISV 150
    SATFEHLVYK IDSDDTQFPP MRCGLTEEKI AHQMELQLSY NFTLKQSSFV 200
    GWWTHQRFVE LVVVVDNIRY LFSQSNATTV QHEVFNVVNI VDSFYHPLEV 250
    DVILTGIDIW TASNPLPTSG DLDNVLEDFS IWKNYNLNNR LQHDVAHLFI 300
    KDTQGMKLGV AYVKGICQNP FNTGVDVFED NRLVVFAITL GHELGHNLGM 350
    QHDTQWCVCE LQWCIMHAYR KVTTKFSNCS YAQYWDSTIS SGLCIQPPPY 400
    PGNIFRLKYC GNLVVEEGEE CDCGTIRQCA KDPCCLLNCT LHPGAACAFG 450
    ICCKDCKFLP SGTLCRQQVG ECDLPEWCNG TSHQCPDDVY VQDGISCNVN 500
    AFCYEKTCNN HDIQCKEIFG QDARSASQSC YQEINTQGNR FGHCGIVGTT 550
    YVKCWTPDIM CGRVQCENVG VIPNLIEHST VQQFHLNDTT CWGTDYHLGM 600
    AIPDIGEVKD GTVCGPEKIC IRKKCASMVH LSQACQPKTC NMRGICNNKQ 650
    HCHCNHEWAP PYCKDKGYGG SADSGPPPKN NMEGLNVMGK LRYLSLLCLL 700
    PLVAFLLFCL HVLFKKRTKS KEDEEG 726
    Length:726
    Mass (Da):81,603
    Last modified:November 25, 2008 - v2
    Checksum:i7DB864FA1796A9B0
    GO

    Sequence cautioni

    The sequence AAH25378.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAH25378.2 differs from that shown. Reason: Erroneous initiation.
    The sequence EAW81037.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti109 – 1091C → W in AAC52041. (PubMed:9469942)Curated
    Sequence conflicti637 – 6371P → R in AAC52041. (PubMed:9469942)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti19 – 191F → L.1 Publication
    Corresponds to variant rs1059166 [ dbSNP | Ensembl ].
    VAR_047311

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF029899 mRNA. Translation: AAC52041.1.
    AF158643 Genomic DNA. Translation: AAD55254.1.
    AL357153 Genomic DNA. No translation available.
    CH471061 Genomic DNA. Translation: EAW81037.1. Different initiation.
    BC025378 mRNA. Translation: AAH25378.2. Different initiation.
    RefSeqiNP_003805.3. NM_003814.4.
    XP_005268208.1. XM_005268151.1.
    XP_005268209.1. XM_005268152.1.
    UniGeneiHs.177984.

    Genome annotation databases

    EnsembliENST00000256389; ENSP00000256389; ENSG00000134007.
    GeneIDi8748.
    KEGGihsa:8748.
    UCSCiuc001xme.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF029899 mRNA. Translation: AAC52041.1 .
    AF158643 Genomic DNA. Translation: AAD55254.1 .
    AL357153 Genomic DNA. No translation available.
    CH471061 Genomic DNA. Translation: EAW81037.1 . Different initiation.
    BC025378 mRNA. Translation: AAH25378.2 . Different initiation.
    RefSeqi NP_003805.3. NM_003814.4.
    XP_005268208.1. XM_005268151.1.
    XP_005268209.1. XM_005268152.1.
    UniGenei Hs.177984.

    3D structure databases

    ProteinModelPortali O43506.
    SMRi O43506. Positions 206-628.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114284. 1 interaction.
    IntActi O43506. 1 interaction.
    MINTi MINT-7970672.
    STRINGi 9606.ENSP00000256389.

    Protein family/group databases

    MEROPSi M12.218.

    PTM databases

    PhosphoSitei O43506.

    Proteomic databases

    PaxDbi O43506.
    PRIDEi O43506.

    Protocols and materials databases

    DNASUi 8748.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000256389 ; ENSP00000256389 ; ENSG00000134007 .
    GeneIDi 8748.
    KEGGi hsa:8748.
    UCSCi uc001xme.3. human.

    Organism-specific databases

    CTDi 8748.
    GeneCardsi GC14M070989.
    HGNCi HGNC:199. ADAM20.
    MIMi 603712. gene.
    neXtProti NX_O43506.
    PharmGKBi PA24516.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG246189.
    HOGENOMi HOG000230883.
    HOVERGENi HBG006978.
    InParanoidi O43506.
    KOi K08609.
    OrthoDBi EOG7KWSGW.
    PhylomeDBi O43506.
    TreeFami TF314733.

    Enzyme and pathway databases

    Reactomei REACT_163933. Interaction With The Zona Pellucida.

    Miscellaneous databases

    GenomeRNAii 8748.
    NextBioi 32821.
    PROi O43506.
    SOURCEi Search...

    Gene expression databases

    Bgeei O43506.
    CleanExi HS_ADAM20.
    Genevestigatori O43506.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProi IPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view ]
    Pfami PF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view ]
    PRINTSi PR00289. DISINTEGRIN.
    SMARTi SM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57552. SSF57552. 1 hit.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ADAM 20 and 21; two novel human testis-specific membrane metalloproteases with similarity to fertilin-alpha."
      Hooft van Huijsduijnen R.
      Gene 206:273-282(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-19.
      Tissue: Testis.
    2. "The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries."
      Poindexter K., Nelson N., DuBose R.F., Black R.A., Cerretti D.P.
      Gene 237:61-70(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiADA20_HUMAN
    AccessioniPrimary (citable) accession number: O43506
    Secondary accession number(s): Q6GTZ1, Q9UKJ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3