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O43506

- ADA20_HUMAN

UniProt

O43506 - ADA20_HUMAN

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Protein

Disintegrin and metalloproteinase domain-containing protein 20

Gene

ADAM20

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

May be involved in sperm maturation and/or fertilization.

Cofactori

Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi173 – 1731Zinc; in inhibited formBy similarity
Metal bindingi342 – 3421Zinc; catalyticSequence Analysis
Active sitei343 – 3431PROSITE-ProRule annotation
Metal bindingi346 – 3461Zinc; catalyticSequence Analysis
Metal bindingi352 – 3521Zinc; catalyticSequence Analysis

GO - Molecular functioni

  1. metalloendopeptidase activity Source: InterPro
  2. metallopeptidase activity Source: ProtInc
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. binding of sperm to zona pellucida Source: Reactome
  2. multicellular organism reproduction Source: Reactome
  3. single fertilization Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_163933. Interaction With The Zona Pellucida.

Protein family/group databases

MEROPSiM12.218.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 20 (EC:3.4.24.-)
Short name:
ADAM 20
Gene namesi
Name:ADAM20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:199. ADAM20.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24516.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Propeptidei32 – 206175Sequence AnalysisPRO_0000029106Add
BLAST
Chaini207 – 726520Disintegrin and metalloproteinase domain-containing protein 20PRO_0000029107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi317 ↔ 394By similarity
Disulfide bondi357 ↔ 379By similarity
Disulfide bondi359 ↔ 364By similarity
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi438 – 4381N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi465 ↔ 485By similarity
Glycosylationi479 – 4791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi587 – 5871N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi635 ↔ 646By similarity
Disulfide bondi640 ↔ 652By similarity
Disulfide bondi654 ↔ 663By similarity

Post-translational modificationi

Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO43506.
PRIDEiO43506.

PTM databases

PhosphoSiteiO43506.

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

BgeeiO43506.
CleanExiHS_ADAM20.
GenevestigatoriO43506.

Interactioni

Protein-protein interaction databases

BioGridi114284. 1 interaction.
IntActiO43506. 1 interaction.
MINTiMINT-7970672.
STRINGi9606.ENSP00000256389.

Structurei

3D structure databases

ProteinModelPortaliO43506.
SMRiO43506. Positions 206-628.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini207 – 693487ExtracellularSequence AnalysisAdd
BLAST
Topological domaini715 – 72612CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei694 – 71421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini207 – 400194Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini407 – 49387DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini635 – 66329EGF-likeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi171 – 1788Cysteine switchBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi494 – 634141Cys-richAdd
BLAST

Domaini

A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG246189.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiO43506.
KOiK08609.
OrthoDBiEOG7KWSGW.
PhylomeDBiO43506.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43506-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVGEPLVHI RVTLLLLWFG MFLSISGHSQ ARPSQYFTSP EVVIPLKVIS
60 70 80 90 100
RGRGAKAPGW LSYSLRFGGQ RYIVHMRVNK LLFAAHLPVF TYTEQHALLQ
110 120 130 140 150
DQPFIQDDCY YHGYVEGVPE SLVALSTCSG GFLGMLQIND LVYEIKPISV
160 170 180 190 200
SATFEHLVYK IDSDDTQFPP MRCGLTEEKI AHQMELQLSY NFTLKQSSFV
210 220 230 240 250
GWWTHQRFVE LVVVVDNIRY LFSQSNATTV QHEVFNVVNI VDSFYHPLEV
260 270 280 290 300
DVILTGIDIW TASNPLPTSG DLDNVLEDFS IWKNYNLNNR LQHDVAHLFI
310 320 330 340 350
KDTQGMKLGV AYVKGICQNP FNTGVDVFED NRLVVFAITL GHELGHNLGM
360 370 380 390 400
QHDTQWCVCE LQWCIMHAYR KVTTKFSNCS YAQYWDSTIS SGLCIQPPPY
410 420 430 440 450
PGNIFRLKYC GNLVVEEGEE CDCGTIRQCA KDPCCLLNCT LHPGAACAFG
460 470 480 490 500
ICCKDCKFLP SGTLCRQQVG ECDLPEWCNG TSHQCPDDVY VQDGISCNVN
510 520 530 540 550
AFCYEKTCNN HDIQCKEIFG QDARSASQSC YQEINTQGNR FGHCGIVGTT
560 570 580 590 600
YVKCWTPDIM CGRVQCENVG VIPNLIEHST VQQFHLNDTT CWGTDYHLGM
610 620 630 640 650
AIPDIGEVKD GTVCGPEKIC IRKKCASMVH LSQACQPKTC NMRGICNNKQ
660 670 680 690 700
HCHCNHEWAP PYCKDKGYGG SADSGPPPKN NMEGLNVMGK LRYLSLLCLL
710 720
PLVAFLLFCL HVLFKKRTKS KEDEEG
Length:726
Mass (Da):81,603
Last modified:November 25, 2008 - v2
Checksum:i7DB864FA1796A9B0
GO

Sequence cautioni

The sequence AAH25378.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAH25378.2 differs from that shown. Reason: Erroneous initiation.
The sequence EAW81037.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091C → W in AAC52041. (PubMed:9469942)Curated
Sequence conflicti637 – 6371P → R in AAC52041. (PubMed:9469942)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti19 – 191F → L.1 Publication
Corresponds to variant rs1059166 [ dbSNP | Ensembl ].
VAR_047311

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF029899 mRNA. Translation: AAC52041.1.
AF158643 Genomic DNA. Translation: AAD55254.1.
AL357153 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81037.1. Different initiation.
BC025378 mRNA. Translation: AAH25378.2. Different initiation.
RefSeqiNP_003805.3. NM_003814.4.
XP_005268208.1. XM_005268151.1.
XP_005268209.1. XM_005268152.1.
UniGeneiHs.177984.

Genome annotation databases

EnsembliENST00000256389; ENSP00000256389; ENSG00000134007.
GeneIDi8748.
KEGGihsa:8748.
UCSCiuc001xme.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF029899 mRNA. Translation: AAC52041.1 .
AF158643 Genomic DNA. Translation: AAD55254.1 .
AL357153 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81037.1 . Different initiation.
BC025378 mRNA. Translation: AAH25378.2 . Different initiation.
RefSeqi NP_003805.3. NM_003814.4.
XP_005268208.1. XM_005268151.1.
XP_005268209.1. XM_005268152.1.
UniGenei Hs.177984.

3D structure databases

ProteinModelPortali O43506.
SMRi O43506. Positions 206-628.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114284. 1 interaction.
IntActi O43506. 1 interaction.
MINTi MINT-7970672.
STRINGi 9606.ENSP00000256389.

Protein family/group databases

MEROPSi M12.218.

PTM databases

PhosphoSitei O43506.

Proteomic databases

PaxDbi O43506.
PRIDEi O43506.

Protocols and materials databases

DNASUi 8748.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000256389 ; ENSP00000256389 ; ENSG00000134007 .
GeneIDi 8748.
KEGGi hsa:8748.
UCSCi uc001xme.3. human.

Organism-specific databases

CTDi 8748.
GeneCardsi GC14M070989.
HGNCi HGNC:199. ADAM20.
MIMi 603712. gene.
neXtProti NX_O43506.
PharmGKBi PA24516.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG246189.
GeneTreei ENSGT00760000118888.
HOGENOMi HOG000230883.
HOVERGENi HBG006978.
InParanoidi O43506.
KOi K08609.
OrthoDBi EOG7KWSGW.
PhylomeDBi O43506.
TreeFami TF314733.

Enzyme and pathway databases

Reactomei REACT_163933. Interaction With The Zona Pellucida.

Miscellaneous databases

GenomeRNAii 8748.
NextBioi 32821.
PROi O43506.
SOURCEi Search...

Gene expression databases

Bgeei O43506.
CleanExi HS_ADAM20.
Genevestigatori O43506.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProi IPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view ]
Pfami PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view ]
PRINTSi PR00289. DISINTEGRIN.
SMARTi SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view ]
SUPFAMi SSF57552. SSF57552. 1 hit.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ADAM 20 and 21; two novel human testis-specific membrane metalloproteases with similarity to fertilin-alpha."
    Hooft van Huijsduijnen R.
    Gene 206:273-282(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-19.
    Tissue: Testis.
  2. "The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries."
    Poindexter K., Nelson N., DuBose R.F., Black R.A., Cerretti D.P.
    Gene 237:61-70(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiADA20_HUMAN
AccessioniPrimary (citable) accession number: O43506
Secondary accession number(s): Q6GTZ1, Q9UKJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 25, 2008
Last modified: October 29, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3