O43506 (ADA20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Disintegrin and metalloproteinase domain-containing protein 20
Short name=ADAM 20
EC=3.4.24.-

Gene names

Name:

ADAM20

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in sperm maturation and/or fertilization.
Cofactor	Binds 1 zinc ion per subunit Potential.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Testis specific.
Domain	A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding. The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
Miscellaneous	May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain.
Sequence caution	The sequence AAH25378.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAH25378.2 differs from that shown. Reason: Erroneous initiation. The sequence EAW81037.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Membrane
Domain	EGF-like domain Signal Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW single fertilization Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Propeptide	32 – 206	175	Potential		PRO_0000029106
	Chain	207 – 726	520	Disintegrin and metalloproteinase domain-containing protein 20		PRO_0000029107
Regions
	Topological domain	207 – 693	487	Extracellular Potential
	Transmembrane	694 – 714	21	Helical; Potential
	Topological domain	715 – 726	12	Cytoplasmic Potential
	Domain	207 – 400	194	Peptidase M12B
	Domain	407 – 493	87	Disintegrin
	Domain	635 – 663	29	EGF-like
	Motif	171 – 178	8	Cysteine switch By similarity
	Compositional bias	494 – 634	141	Cys-rich
Sites
	Active site	343	1	Potential
	Metal binding	173	1	Zinc; in inhibited form By similarity
	Metal binding	342	1	Zinc; catalytic Potential
	Metal binding	346	1	Zinc; catalytic Potential
	Metal binding	352	1	Zinc; catalytic Potential
Amino acid modifications
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	226	1	N-linked (GlcNAc...) Potential
	Glycosylation	378	1	N-linked (GlcNAc...) Potential
	Glycosylation	438	1	N-linked (GlcNAc...) Potential
	Glycosylation	479	1	N-linked (GlcNAc...) Potential
	Glycosylation	587	1	N-linked (GlcNAc...) Potential
	Disulfide bond	317 ↔ 394		By similarity
	Disulfide bond	357 ↔ 379		By similarity
	Disulfide bond	359 ↔ 364		By similarity
	Disulfide bond	465 ↔ 485		By similarity
	Disulfide bond	635 ↔ 646		By similarity
	Disulfide bond	640 ↔ 652		By similarity
	Disulfide bond	654 ↔ 663		By similarity
Natural variations
	Natural variant	19	1	F → L. Ref.1 Corresponds to variant rs1059166 [ dbSNP | Ensembl ].		VAR_047311
Experimental info
	Sequence conflict	109	1	C → W in AAC52041. Ref.1
	Sequence conflict	637	1	P → R in AAC52041. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O43506 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 7DB864FA1796A9B0	FASTA	726	81,603
	10 20 30 40 50 60 MAVGEPLVHI RVTLLLLWFG MFLSISGHSQ ARPSQYFTSP EVVIPLKVIS RGRGAKAPGW 70 80 90 100 110 120 LSYSLRFGGQ RYIVHMRVNK LLFAAHLPVF TYTEQHALLQ DQPFIQDDCY YHGYVEGVPE 130 140 150 160 170 180 SLVALSTCSG GFLGMLQIND LVYEIKPISV SATFEHLVYK IDSDDTQFPP MRCGLTEEKI 190 200 210 220 230 240 AHQMELQLSY NFTLKQSSFV GWWTHQRFVE LVVVVDNIRY LFSQSNATTV QHEVFNVVNI 250 260 270 280 290 300 VDSFYHPLEV DVILTGIDIW TASNPLPTSG DLDNVLEDFS IWKNYNLNNR LQHDVAHLFI 310 320 330 340 350 360 KDTQGMKLGV AYVKGICQNP FNTGVDVFED NRLVVFAITL GHELGHNLGM QHDTQWCVCE 370 380 390 400 410 420 LQWCIMHAYR KVTTKFSNCS YAQYWDSTIS SGLCIQPPPY PGNIFRLKYC GNLVVEEGEE 430 440 450 460 470 480 CDCGTIRQCA KDPCCLLNCT LHPGAACAFG ICCKDCKFLP SGTLCRQQVG ECDLPEWCNG 490 500 510 520 530 540 TSHQCPDDVY VQDGISCNVN AFCYEKTCNN HDIQCKEIFG QDARSASQSC YQEINTQGNR 550 560 570 580 590 600 FGHCGIVGTT YVKCWTPDIM CGRVQCENVG VIPNLIEHST VQQFHLNDTT CWGTDYHLGM 610 620 630 640 650 660 AIPDIGEVKD GTVCGPEKIC IRKKCASMVH LSQACQPKTC NMRGICNNKQ HCHCNHEWAP 670 680 690 700 710 720 PYCKDKGYGG SADSGPPPKN NMEGLNVMGK LRYLSLLCLL PLVAFLLFCL HVLFKKRTKS KEDEEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ADAM 20 and 21; two novel human testis-specific membrane metalloproteases with similarity to fertilin-alpha." Hooft van Huijsduijnen R. Gene 206:273-282(1998) [PubMed: 9469942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-19. Tissue: Testis.
[2]	"The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries." Poindexter K., Nelson N., DuBose R.F., Black R.A., Cerretti D.P. Gene 237:61-70(1999) [PubMed: 10524237] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF029899 mRNA. Translation: AAC52041.1. AF158643 Genomic DNA. Translation: AAD55254.1. AL357153 Genomic DNA. No translation available. CH471061 Genomic DNA. Translation: EAW81037.1. Different initiation. BC025378 mRNA. Translation: AAH25378.2. Different initiation.
IPI	IPI00306205.
RefSeq	NP_003805.3. NM_003814.4.
UniGene	Hs.177984.
3D structure databases
ProteinModelPortal	O43506.
SMR	O43506. Positions 202-670.
ModBase	Search...
Protein-protein interaction databases
STRING	O43506.
Protein family/group databases
MEROPS	M12.218.
Proteomic databases
PRIDE	O43506.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000256389; ENSP00000256389; ENSG00000134007.
GeneID	8748.
KEGG	hsa:8748.
Organism-specific databases
CTD	8748.
GeneCards	GC14M070989.
H-InvDB	HIX0202085.
HGNC	HGNC:199. ADAM20.
MIM	603712. gene.
neXtProt	NX_O43506.
PharmGKB	PA24516.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15722.
GeneTree	ENSGT00590000082741.
HOGENOM	HBG507118.
HOVERGEN	HBG006978.
InParanoid	O43506.
OrthoDB	EOG4XWFX8.
PhylomeDB	O43506.
Gene expression databases
ArrayExpress	O43506.
Bgee	O43506.
CleanEx	HS_ADAM20.
Genevestigator	O43506.
GermOnline	ENSG00000134007. Homo sapiens.
Family and domain databases
InterPro	IPR006586. ADAM_Cys-rich. IPR001762. Blood-coag_inhib_Disintegrin. IPR018358. Disintegrin_CS. IPR024079. MetalloPept_cat_dom. IPR001590. Peptidase_M12B. IPR002870. Peptidase_M12B_N. [Graphical view]
Gene3D	G3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit. G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
KO	K08609.
Pfam	PF08516. ADAM_CR. 1 hit. PF00200. Disintegrin. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. [Graphical view]
PRINTS	PR00289. DISINTEGRIN.
SMART	SM00608. ACR. 1 hit. SM00050. DISIN. 1 hit. [Graphical view]
SUPFAM	SSF57552. Disintegrin. 1 hit.
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00546. CYSTEINE_SWITCH. False negative. PS00427. DISINTEGRIN_1. 1 hit. PS50214. DISINTEGRIN_2. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

ADA20_HUMAN

Accession

Primary (citable) accession number: O43506
Secondary accession number(s): Q6GTZ1, Q9UKJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.