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Protein

Disintegrin and metalloproteinase domain-containing protein 20

Gene

ADAM20

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May be involved in sperm maturation and/or fertilization.

Miscellaneous

May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi173Zinc; in inhibited formBy similarity1
Metal bindingi342Zinc; catalyticSequence analysis1
Active sitei343PROSITE-ProRule annotation1
Metal bindingi346Zinc; catalyticSequence analysis1
Metal bindingi352Zinc; catalyticSequence analysis1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: ProtInc

GO - Biological processi

  • binding of sperm to zona pellucida Source: Reactome
  • single fertilization Source: ProtInc

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1300644 Interaction With The Zona Pellucida

Protein family/group databases

MEROPSiM12.218

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 20 (EC:3.4.24.-)
Short name:
ADAM 20
Gene namesi
Name:ADAM20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000134007.3
HGNCiHGNC:199 ADAM20
MIMi603712 gene
neXtProtiNX_O43506

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini207 – 693ExtracellularSequence analysisAdd BLAST487
Transmembranei694 – 714HelicalSequence analysisAdd BLAST21
Topological domaini715 – 726CytoplasmicSequence analysisAdd BLAST12

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8748
PharmGKBiPA24516

Polymorphism and mutation databases

BioMutaiADAM20

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
PropeptideiPRO_000002910632 – 206Sequence analysisAdd BLAST175
ChainiPRO_0000029107207 – 726Disintegrin and metalloproteinase domain-containing protein 20Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi191N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi226N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi317 ↔ 394By similarity
Disulfide bondi357 ↔ 379By similarity
Disulfide bondi359 ↔ 364By similarity
Glycosylationi378N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi438N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi465 ↔ 485By similarity
Glycosylationi479N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi587N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi635 ↔ 646By similarity
Disulfide bondi640 ↔ 652By similarity
Disulfide bondi654 ↔ 663By similarity

Post-translational modificationi

Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO43506
PeptideAtlasiO43506
PRIDEiO43506

PTM databases

iPTMnetiO43506
PhosphoSitePlusiO43506

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

BgeeiENSG00000134007
CleanExiHS_ADAM20
GenevisibleiO43506 HS

Organism-specific databases

HPAiHPA059377

Interactioni

Protein-protein interaction databases

IntActiO43506, 1 interactor
MINTiO43506
STRINGi9606.ENSP00000256389

Structurei

3D structure databases

ProteinModelPortaliO43506
SMRiO43506
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 400Peptidase M12BPROSITE-ProRule annotationAdd BLAST194
Domaini407 – 493DisintegrinPROSITE-ProRule annotationAdd BLAST87
Domaini635 – 663EGF-likeAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi171 – 178Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi494 – 634Cys-richAdd BLAST141

Domaini

A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607 Eukaryota
ENOG410XX2M LUCA
HOGENOMiHOG000230883
HOVERGENiHBG006978
InParanoidiO43506
KOiK08609
OrthoDBiEOG091G03BZ
PhylomeDBiO43506
TreeFamiTF314733

Family and domain databases

CDDicd04269 ZnMc_adamalysin_II_like, 1 hit
Gene3Di3.40.390.10, 1 hit
4.10.70.10, 1 hit
InterProiView protein in InterPro
IPR006586 ADAM_Cys-rich
IPR018358 Disintegrin_CS
IPR001762 Disintegrin_dom
IPR036436 Disintegrin_dom_sf
IPR024079 MetalloPept_cat_dom_sf
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR034027 Reprolysin_adamalysin
PfamiView protein in Pfam
PF08516 ADAM_CR, 1 hit
PF00200 Disintegrin, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF01421 Reprolysin, 1 hit
PRINTSiPR00289 DISINTEGRIN
SMARTiView protein in SMART
SM00608 ACR, 1 hit
SM00050 DISIN, 1 hit
SUPFAMiSSF57552 SSF57552, 1 hit
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS00427 DISINTEGRIN_1, 1 hit
PS50214 DISINTEGRIN_2, 1 hit
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVGEPLVHI RVTLLLLWFG MFLSISGHSQ ARPSQYFTSP EVVIPLKVIS
60 70 80 90 100
RGRGAKAPGW LSYSLRFGGQ RYIVHMRVNK LLFAAHLPVF TYTEQHALLQ
110 120 130 140 150
DQPFIQDDCY YHGYVEGVPE SLVALSTCSG GFLGMLQIND LVYEIKPISV
160 170 180 190 200
SATFEHLVYK IDSDDTQFPP MRCGLTEEKI AHQMELQLSY NFTLKQSSFV
210 220 230 240 250
GWWTHQRFVE LVVVVDNIRY LFSQSNATTV QHEVFNVVNI VDSFYHPLEV
260 270 280 290 300
DVILTGIDIW TASNPLPTSG DLDNVLEDFS IWKNYNLNNR LQHDVAHLFI
310 320 330 340 350
KDTQGMKLGV AYVKGICQNP FNTGVDVFED NRLVVFAITL GHELGHNLGM
360 370 380 390 400
QHDTQWCVCE LQWCIMHAYR KVTTKFSNCS YAQYWDSTIS SGLCIQPPPY
410 420 430 440 450
PGNIFRLKYC GNLVVEEGEE CDCGTIRQCA KDPCCLLNCT LHPGAACAFG
460 470 480 490 500
ICCKDCKFLP SGTLCRQQVG ECDLPEWCNG TSHQCPDDVY VQDGISCNVN
510 520 530 540 550
AFCYEKTCNN HDIQCKEIFG QDARSASQSC YQEINTQGNR FGHCGIVGTT
560 570 580 590 600
YVKCWTPDIM CGRVQCENVG VIPNLIEHST VQQFHLNDTT CWGTDYHLGM
610 620 630 640 650
AIPDIGEVKD GTVCGPEKIC IRKKCASMVH LSQACQPKTC NMRGICNNKQ
660 670 680 690 700
HCHCNHEWAP PYCKDKGYGG SADSGPPPKN NMEGLNVMGK LRYLSLLCLL
710 720
PLVAFLLFCL HVLFKKRTKS KEDEEG
Length:726
Mass (Da):81,603
Last modified:November 25, 2008 - v2
Checksum:i7DB864FA1796A9B0
GO

Sequence cautioni

The sequence AAH25378 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH25378 differs from that shown. Reason: Erroneous initiation.Curated
The sequence EAW81037 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109C → W in AAC52041 (PubMed:9469942).Curated1
Sequence conflicti637P → R in AAC52041 (PubMed:9469942).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04731119F → L1 PublicationCorresponds to variant dbSNP:rs1059166Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029899 mRNA Translation: AAC52041.1
AF158643 Genomic DNA Translation: AAD55254.1
AL357153 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW81037.1 Different initiation.
BC025378 mRNA Translation: AAH25378.2 Different initiation.
RefSeqiNP_003805.3, NM_003814.4
XP_005268208.1, XM_005268151.3
UniGeneiHs.177984

Genome annotation databases

EnsembliENST00000256389; ENSP00000256389; ENSG00000134007
GeneIDi8748
KEGGihsa:8748
UCSCiuc001xme.4 human

Similar proteinsi

Entry informationi

Entry nameiADA20_HUMAN
AccessioniPrimary (citable) accession number: O43506
Secondary accession number(s): Q6GTZ1, Q9UKJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 25, 2008
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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