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O43482 (MS18B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Mis18-beta
Alternative name(s):
Cancer/testis antigen 86
Short name=CT86
Opa-interacting protein 5
Short name=OIP-5
Gene names
Name:OIP5
Synonyms:MIS18B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. Ref.3

Subunit structure

Homodimer, and heterodimer with MIS18A. Identified in a complex containing MIS18A, MIS18B, MIS18BP1, RBBP7 and RBBP4. Binds outer membrane protein OpaP from Neisseria gonorrhoeae. Ref.3

Subcellular location

Nucleus. Chromosome. Chromosomecentromere. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin. Ref.3

Sequence caution

The sequence AAC39561.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RAF1P040494EBI-536879,EBI-365996

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229Protein Mis18-beta
PRO_0000058038

Amino acid modifications

Modified residue481Phosphoserine Ref.6
Modified residue2211Phosphothreonine Ref.6 Ref.7
Modified residue2251Phosphoserine Ref.6 Ref.7 Ref.8

Sequences

Sequence LengthMass (Da)Tools
O43482 [UniParc].

Last modified April 3, 2002. Version 2.
Checksum: 0EBD4006193A3106

FASTA22924,691
        10         20         30         40         50         60 
MAAQPLRHRS RCATPPRGDF CGGTERAIDQ ASFTTSMEWD TQVVKGSSPL GPAGLGAEEP 

        70         80         90        100        110        120 
AAGPQLPSWL QPERCAVFQC AQCHAVLADS VHLAWDLSRS LGAVVFSRVT NNVVLEAPFL 

       130        140        150        160        170        180 
VGIEGSLKGS TYNLLFCGSC GIPVGFHLYS THAALAALRG HFCLSSDKMV CYLLKTKAIV 

       190        200        210        220 
NASEMDIQNV PLSEKIAELK EKIVLTHNRL KSLMKILSEV TPDQSKPEN 

« Hide

References

« Hide 'large scale' references
[1]"Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth."
Williams J.M., Chen G.-C., Zhu L., Rest R.F.
Mol. Microbiol. 27:171-186(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Uterus.
[3]"Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1."
Fujita Y., Hayashi T., Kiyomitsu T., Toyoda Y., Kokubu A., Obuse C., Yanagida M.
Dev. Cell 12:17-30(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MIS18A, IDENTIFICATION IN A COMPLEX CONTAINING MIS18A; MIS18B; MIS18BP1; RBBP7 AND RBBP4.
[4]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; THR-221 AND SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-221 AND SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF025441 mRNA. Translation: AAC39561.1. Different initiation.
BC015050 mRNA. Translation: AAH15050.1.
CCDSCCDS10074.1.
RefSeqNP_009211.1. NM_007280.1.
UniGeneHs.661645.

3D structure databases

ProteinModelPortalO43482.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116467. 15 interactions.
IntActO43482. 10 interactions.
MINTMINT-8417709.
STRING9606.ENSP00000220514.

PTM databases

PhosphoSiteO43482.

Proteomic databases

MaxQBO43482.
PaxDbO43482.
PRIDEO43482.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000220514; ENSP00000220514; ENSG00000104147.
GeneID11339.
KEGGhsa:11339.
UCSCuc001znp.3. human.

Organism-specific databases

CTD11339.
GeneCardsGC15M041601.
HGNCHGNC:20300. OIP5.
HPAHPA052271.
MIM606020. gene.
neXtProtNX_O43482.
PharmGKBPA134887641.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG79271.
HOGENOMHOG000113655.
HOVERGENHBG095583.
InParanoidO43482.
KOK11565.
OMANCETMAN.
OrthoDBEOG7VMP80.
PhylomeDBO43482.
TreeFamTF330739.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.

Gene expression databases

BgeeO43482.
CleanExHS_OIP5.
GenevestigatorO43482.

Family and domain databases

InterProIPR004910. Yippee/Mis18.
[Graphical view]
PfamPF03226. Yippee-Mis18. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSOIP5. human.
GenomeRNAi11339.
NextBio43085.
PROO43482.
SOURCESearch...

Entry information

Entry nameMS18B_HUMAN
AccessionPrimary (citable) accession number: O43482
Secondary accession number(s): Q96BX7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: April 3, 2002
Last modified: July 9, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM