O43482 (MS18B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Mis18-beta

Alternative name(s):
Cancer/testis antigen 86
Short name=CT86
Opa-interacting protein 5
Short name=OIP-5

Gene names

Name:	OIP5
Synonyms:	MIS18B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. Ref.3
Subunit structure	Homodimer, and heterodimer with MIS18A. Identified in a complex containing MIS18A, MIS18B, MIS18BP1, RBBP7 and RBBP4. Binds outer membrane protein OpaP from Neisseria gonorrhoeae. Ref.3
Subcellular location	Nucleus. Chromosome. Chromosome › centromere. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin. Ref.3
Sequence caution	The sequence AAC39561.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CENP-A containing nucleosome assembly at centromere Traceable author statement. Source: Reactome cell communication Non-traceable author statement Ref.1. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Cajal body Inferred from direct assay PubMed 17284516. Source: MGI chromatin Inferred from direct assay PubMed 17284516. Source: MGI chromocenter Inferred from electronic annotation. Source: Compara chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RAF1	P04049	4	EBI-536879,EBI-365996

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Protein Mis18-beta		PRO_0000058038
Amino acid modifications
	Modified residue	48	1	Phosphoserine Ref.6
	Modified residue	221	1	Phosphothreonine Ref.6 Ref.7
	Modified residue	225	1	Phosphoserine Ref.6 Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	O43482 [UniParc]. Last modified April 3, 2002. Version 2. Checksum: 0EBD4006193A3106	FASTA	229	24,691
	10 20 30 40 50 60 MAAQPLRHRS RCATPPRGDF CGGTERAIDQ ASFTTSMEWD TQVVKGSSPL GPAGLGAEEP 70 80 90 100 110 120 AAGPQLPSWL QPERCAVFQC AQCHAVLADS VHLAWDLSRS LGAVVFSRVT NNVVLEAPFL 130 140 150 160 170 180 VGIEGSLKGS TYNLLFCGSC GIPVGFHLYS THAALAALRG HFCLSSDKMV CYLLKTKAIV 190 200 210 220 NASEMDIQNV PLSEKIAELK EKIVLTHNRL KSLMKILSEV TPDQSKPEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth." Williams J.M., Chen G.-C., Zhu L., Rest R.F. Mol. Microbiol. 27:171-186(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[3]	"Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1." Fujita Y., Hayashi T., Kiyomitsu T., Toyoda Y., Kokubu A., Obuse C., Yanagida M. Dev. Cell 12:17-30(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, INTERACTION WITH MIS18A, IDENTIFICATION IN A COMPLEX CONTAINING MIS18A; MIS18B; MIS18BP1; RBBP7 AND RBBP4.
[4]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; THR-221 AND SER-225, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-221 AND SER-225, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF025441 mRNA. Translation: AAC39561.1. Different initiation. BC015050 mRNA. Translation: AAH15050.1.
IPI	IPI00001999.
RefSeq	NP_009211.1. NM_007280.1.
UniGene	Hs.661645.
3D structure databases
ProteinModelPortal	O43482.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43482. 8 interactions.
STRING	9606.ENSP00000220514.
PTM databases
PhosphoSite	O43482.
Proteomic databases
PaxDb	O43482.
PRIDE	O43482.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000220514; ENSP00000220514; ENSG00000104147.
GeneID	11339.
KEGG	hsa:11339.
UCSC	uc001znp.3. human.
Organism-specific databases
CTD	11339.
GeneCards	GC15M041601.
HGNC	HGNC:20300. OIP5.
HPA	HPA052271.
MIM	606020. gene.
neXtProt	NX_O43482.
PharmGKB	PA134887641.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG79271.
HOGENOM	HOG000113655.
HOVERGEN	HBG095583.
InParanoid	O43482.
KO	K11565.
OMA	FTTSMEW.
OrthoDB	EOG45MN68.
PhylomeDB	O43482.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle.
Gene expression databases
Bgee	O43482.
CleanEx	HS_OIP5.
Genevestigator	O43482.
GermOnline	ENSG00000104147. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	OIP5. human.
GenomeRNAi	11339.
NextBio	43085.
SOURCE	Search...

Entry information

Entry name

MS18B_HUMAN

Accession

Primary (citable) accession number: O43482
Secondary accession number(s): Q96BX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	April 3, 2002
Last modified:	April 3, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.