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Protein

Protein Mis18-beta

Gene

OIP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.1 Publication

GO - Biological processi

  1. cell communication Source: UniProtKB
  2. cell division Source: UniProtKB-KW
  3. CENP-A containing nucleosome assembly Source: Reactome
  4. mitotic nuclear division Source: UniProtKB-KW
  5. nucleosome assembly Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Mis18-beta
Alternative name(s):
Cancer/testis antigen 86
Short name:
CT86
Opa-interacting protein 5
Short name:
OIP-5
Gene namesi
Name:OIP5
Synonyms:MIS18B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:20300. OIP5.

Subcellular locationi

Nucleus 1 Publication. Chromosome 1 Publication. Chromosomecentromere 1 Publication
Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.

GO - Cellular componenti

  1. Cajal body Source: MGI
  2. chromatin Source: MGI
  3. chromocenter Source: Ensembl
  4. chromosome, centromeric region Source: UniProtKB-SubCell
  5. cytoplasm Source: HPA
  6. nucleoplasm Source: HPA
  7. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134887641.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 229229Protein Mis18-betaPRO_0000058038Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481Phosphoserine1 Publication
Modified residuei221 – 2211Phosphothreonine2 Publications
Modified residuei225 – 2251Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO43482.
PaxDbiO43482.
PRIDEiO43482.

PTM databases

PhosphoSiteiO43482.

Expressioni

Gene expression databases

BgeeiO43482.
CleanExiHS_OIP5.
ExpressionAtlasiO43482. baseline and differential.
GenevestigatoriO43482.

Organism-specific databases

HPAiHPA052271.
HPA059602.

Interactioni

Subunit structurei

Homodimer, and heterodimer with MIS18A. Identified in a complex containing MIS18A, MIS18B, MIS18BP1, RBBP7 and RBBP4. Binds outer membrane protein OpaP from Neisseria gonorrhoeae.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MIS18AQ9NYP96EBI-536879,EBI-1104552
MIS18BP1Q6P0N03EBI-536879,EBI-9870821
RAF1P040494EBI-536879,EBI-365996

Protein-protein interaction databases

BioGridi116467. 38 interactions.
IntActiO43482. 11 interactions.
MINTiMINT-8417709.
STRINGi9606.ENSP00000220514.

Structurei

3D structure databases

ProteinModelPortaliO43482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG79271.
GeneTreeiENSGT00390000007347.
HOGENOMiHOG000113655.
HOVERGENiHBG095583.
InParanoidiO43482.
KOiK11565.
OMAiKIMLTHT.
OrthoDBiEOG7VMP80.
PhylomeDBiO43482.
TreeFamiTF330739.

Family and domain databases

InterProiIPR004910. Yippee/Mis18.
[Graphical view]
PfamiPF03226. Yippee-Mis18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQPLRHRS RCATPPRGDF CGGTERAIDQ ASFTTSMEWD TQVVKGSSPL
60 70 80 90 100
GPAGLGAEEP AAGPQLPSWL QPERCAVFQC AQCHAVLADS VHLAWDLSRS
110 120 130 140 150
LGAVVFSRVT NNVVLEAPFL VGIEGSLKGS TYNLLFCGSC GIPVGFHLYS
160 170 180 190 200
THAALAALRG HFCLSSDKMV CYLLKTKAIV NASEMDIQNV PLSEKIAELK
210 220
EKIVLTHNRL KSLMKILSEV TPDQSKPEN
Length:229
Mass (Da):24,691
Last modified:April 3, 2002 - v2
Checksum:i0EBD4006193A3106
GO

Sequence cautioni

The sequence AAC39561.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025441 mRNA. Translation: AAC39561.1. Different initiation.
BC015050 mRNA. Translation: AAH15050.1.
CCDSiCCDS10074.1.
RefSeqiNP_009211.1. NM_007280.1.
UniGeneiHs.661645.

Genome annotation databases

EnsembliENST00000220514; ENSP00000220514; ENSG00000104147.
GeneIDi11339.
KEGGihsa:11339.
UCSCiuc001znp.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025441 mRNA. Translation: AAC39561.1. Different initiation.
BC015050 mRNA. Translation: AAH15050.1.
CCDSiCCDS10074.1.
RefSeqiNP_009211.1. NM_007280.1.
UniGeneiHs.661645.

3D structure databases

ProteinModelPortaliO43482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116467. 38 interactions.
IntActiO43482. 11 interactions.
MINTiMINT-8417709.
STRINGi9606.ENSP00000220514.

PTM databases

PhosphoSiteiO43482.

Proteomic databases

MaxQBiO43482.
PaxDbiO43482.
PRIDEiO43482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000220514; ENSP00000220514; ENSG00000104147.
GeneIDi11339.
KEGGihsa:11339.
UCSCiuc001znp.3. human.

Organism-specific databases

CTDi11339.
GeneCardsiGC15M041601.
HGNCiHGNC:20300. OIP5.
HPAiHPA052271.
HPA059602.
MIMi606020. gene.
neXtProtiNX_O43482.
PharmGKBiPA134887641.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG79271.
GeneTreeiENSGT00390000007347.
HOGENOMiHOG000113655.
HOVERGENiHBG095583.
InParanoidiO43482.
KOiK11565.
OMAiKIMLTHT.
OrthoDBiEOG7VMP80.
PhylomeDBiO43482.
TreeFamiTF330739.

Enzyme and pathway databases

ReactomeiREACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.

Miscellaneous databases

ChiTaRSiOIP5. human.
GenomeRNAii11339.
NextBioi43085.
PROiO43482.
SOURCEiSearch...

Gene expression databases

BgeeiO43482.
CleanExiHS_OIP5.
ExpressionAtlasiO43482. baseline and differential.
GenevestigatoriO43482.

Family and domain databases

InterProiIPR004910. Yippee/Mis18.
[Graphical view]
PfamiPF03226. Yippee-Mis18. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth."
    Williams J.M., Chen G.-C., Zhu L., Rest R.F.
    Mol. Microbiol. 27:171-186(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterus.
  3. "Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1."
    Fujita Y., Hayashi T., Kiyomitsu T., Toyoda Y., Kokubu A., Obuse C., Yanagida M.
    Dev. Cell 12:17-30(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MIS18A, IDENTIFICATION IN A COMPLEX CONTAINING MIS18A; MIS18B; MIS18BP1; RBBP7 AND RBBP4.
  4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; THR-221 AND SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-221 AND SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMS18B_HUMAN
AccessioniPrimary (citable) accession number: O43482
Secondary accession number(s): Q96BX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: April 3, 2002
Last modified: March 4, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.