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O43482

- MS18B_HUMAN

UniProt

O43482 - MS18B_HUMAN

Protein

Protein Mis18-beta

Gene

OIP5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (03 Apr 2002)
      Previous versions | rss
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    Functioni

    Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cell communication Source: UniProtKB
    2. CENP-A containing nucleosome assembly Source: Reactome
    3. mitotic nuclear division Source: UniProtKB-KW
    4. nucleosome assembly Source: Reactome

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein Mis18-beta
    Alternative name(s):
    Cancer/testis antigen 86
    Short name:
    CT86
    Opa-interacting protein 5
    Short name:
    OIP-5
    Gene namesi
    Name:OIP5
    Synonyms:MIS18B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:20300. OIP5.

    Subcellular locationi

    Nucleus 1 Publication. Chromosome 1 Publication. Chromosomecentromere 1 Publication
    Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.

    GO - Cellular componenti

    1. Cajal body Source: MGI
    2. chromatin Source: MGI
    3. chromocenter Source: Ensembl
    4. chromosome, centromeric region Source: UniProtKB-SubCell
    5. cytoplasm Source: HPA
    6. nucleoplasm Source: Reactome
    7. nucleus Source: HPA

    Keywords - Cellular componenti

    Centromere, Chromosome, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134887641.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 229229Protein Mis18-betaPRO_0000058038Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei48 – 481Phosphoserine1 Publication
    Modified residuei221 – 2211Phosphothreonine2 Publications
    Modified residuei225 – 2251Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO43482.
    PaxDbiO43482.
    PRIDEiO43482.

    PTM databases

    PhosphoSiteiO43482.

    Expressioni

    Gene expression databases

    BgeeiO43482.
    CleanExiHS_OIP5.
    GenevestigatoriO43482.

    Organism-specific databases

    HPAiHPA052271.

    Interactioni

    Subunit structurei

    Homodimer, and heterodimer with MIS18A. Identified in a complex containing MIS18A, MIS18B, MIS18BP1, RBBP7 and RBBP4. Binds outer membrane protein OpaP from Neisseria gonorrhoeae.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RAF1P040494EBI-536879,EBI-365996

    Protein-protein interaction databases

    BioGridi116467. 15 interactions.
    IntActiO43482. 10 interactions.
    MINTiMINT-8417709.
    STRINGi9606.ENSP00000220514.

    Structurei

    3D structure databases

    ProteinModelPortaliO43482.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG79271.
    HOGENOMiHOG000113655.
    HOVERGENiHBG095583.
    InParanoidiO43482.
    KOiK11565.
    OMAiNCETMAN.
    OrthoDBiEOG7VMP80.
    PhylomeDBiO43482.
    TreeFamiTF330739.

    Family and domain databases

    InterProiIPR004910. Yippee/Mis18.
    [Graphical view]
    PfamiPF03226. Yippee-Mis18. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O43482-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAQPLRHRS RCATPPRGDF CGGTERAIDQ ASFTTSMEWD TQVVKGSSPL    50
    GPAGLGAEEP AAGPQLPSWL QPERCAVFQC AQCHAVLADS VHLAWDLSRS 100
    LGAVVFSRVT NNVVLEAPFL VGIEGSLKGS TYNLLFCGSC GIPVGFHLYS 150
    THAALAALRG HFCLSSDKMV CYLLKTKAIV NASEMDIQNV PLSEKIAELK 200
    EKIVLTHNRL KSLMKILSEV TPDQSKPEN 229
    Length:229
    Mass (Da):24,691
    Last modified:April 3, 2002 - v2
    Checksum:i0EBD4006193A3106
    GO

    Sequence cautioni

    The sequence AAC39561.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF025441 mRNA. Translation: AAC39561.1. Different initiation.
    BC015050 mRNA. Translation: AAH15050.1.
    CCDSiCCDS10074.1.
    RefSeqiNP_009211.1. NM_007280.1.
    UniGeneiHs.661645.

    Genome annotation databases

    EnsembliENST00000220514; ENSP00000220514; ENSG00000104147.
    GeneIDi11339.
    KEGGihsa:11339.
    UCSCiuc001znp.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF025441 mRNA. Translation: AAC39561.1 . Different initiation.
    BC015050 mRNA. Translation: AAH15050.1 .
    CCDSi CCDS10074.1.
    RefSeqi NP_009211.1. NM_007280.1.
    UniGenei Hs.661645.

    3D structure databases

    ProteinModelPortali O43482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116467. 15 interactions.
    IntActi O43482. 10 interactions.
    MINTi MINT-8417709.
    STRINGi 9606.ENSP00000220514.

    PTM databases

    PhosphoSitei O43482.

    Proteomic databases

    MaxQBi O43482.
    PaxDbi O43482.
    PRIDEi O43482.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000220514 ; ENSP00000220514 ; ENSG00000104147 .
    GeneIDi 11339.
    KEGGi hsa:11339.
    UCSCi uc001znp.3. human.

    Organism-specific databases

    CTDi 11339.
    GeneCardsi GC15M041601.
    HGNCi HGNC:20300. OIP5.
    HPAi HPA052271.
    MIMi 606020. gene.
    neXtProti NX_O43482.
    PharmGKBi PA134887641.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG79271.
    HOGENOMi HOG000113655.
    HOVERGENi HBG095583.
    InParanoidi O43482.
    KOi K11565.
    OMAi NCETMAN.
    OrthoDBi EOG7VMP80.
    PhylomeDBi O43482.
    TreeFami TF330739.

    Enzyme and pathway databases

    Reactomei REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.

    Miscellaneous databases

    ChiTaRSi OIP5. human.
    GenomeRNAii 11339.
    NextBioi 43085.
    PROi O43482.
    SOURCEi Search...

    Gene expression databases

    Bgeei O43482.
    CleanExi HS_OIP5.
    Genevestigatori O43482.

    Family and domain databases

    InterProi IPR004910. Yippee/Mis18.
    [Graphical view ]
    Pfami PF03226. Yippee-Mis18. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth."
      Williams J.M., Chen G.-C., Zhu L., Rest R.F.
      Mol. Microbiol. 27:171-186(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Uterus.
    3. "Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1."
      Fujita Y., Hayashi T., Kiyomitsu T., Toyoda Y., Kokubu A., Obuse C., Yanagida M.
      Dev. Cell 12:17-30(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MIS18A, IDENTIFICATION IN A COMPLEX CONTAINING MIS18A; MIS18B; MIS18BP1; RBBP7 AND RBBP4.
    4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48; THR-221 AND SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-221 AND SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMS18B_HUMAN
    AccessioniPrimary (citable) accession number: O43482
    Secondary accession number(s): Q96BX7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2002
    Last sequence update: April 3, 2002
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3