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O43474 (KLF4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Krueppel-like factor 4
Alternative name(s):
Epithelial zinc finger protein EZF
Gut-enriched krueppel-like factor
Gene names
Name:KLF4
Synonyms:EZF, GKLF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. Ref.10 Ref.15

Subunit structure

Interacts with POU5F1/OCT4 and SOX2 By similarity. Interacts with MUC1 (via the C-terminal domain). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a Ref.10 Ref.17

Subcellular location

Nucleus.

Domain

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.12

Biotechnological use

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Ref.11

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Sequence caution

The sequence AAB48399.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAC03462.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAD42165.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAH29923.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAH30811.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence ABG25917.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAG36271.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence EAW59020.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EAW59021.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to cycloheximide

Inferred from electronic annotation. Source: Ensembl

cellular response to growth factor stimulus

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

cellular response to hydrogen peroxide

Inferred from electronic annotation. Source: Ensembl

cellular response to peptide

Inferred from electronic annotation. Source: Ensembl

epidermal cell differentiation

Inferred from electronic annotation. Source: Ensembl

epidermis morphogenesis

Inferred from electronic annotation. Source: Ensembl

fat cell differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

mesodermal cell fate determination

Traceable author statement PubMed 8940147. Source: ProtInc

negative regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

negative regulation of NF-kappaB transcription factor activity

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

negative regulation of cell migration involved in sprouting angiogenesis

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

negative regulation of cell proliferation

Traceable author statement PubMed 8702718. Source: ProtInc

negative regulation of chemokine (C-X-C motif) ligand 2 production

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

negative regulation of heterotypic cell-cell adhesion

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

negative regulation of inflammatory response

Traceable author statement PubMed 20551324. Source: BHF-UCL

negative regulation of interleukin-8 biosynthetic process

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

negative regulation of muscle hyperplasia

Inferred from electronic annotation. Source: Ensembl

negative regulation of phosphatidylinositol 3-kinase signaling

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

negative regulation of response to cytokine stimulus

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

negative regulation of smooth muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 20551324. Source: BHF-UCL

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.1. Source: UniProtKB

positive regulation of hemoglobin biosynthetic process

Inferred from mutant phenotype PubMed 21539536. Source: BHF-UCL

positive regulation of protein metabolic process

Inferred from genetic interaction PubMed 20551324. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction. Source: BHF-UCL

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

post-embryonic camera-type eye development

Inferred from electronic annotation. Source: Ensembl

post-embryonic hemopoiesis

Inferred from mutant phenotype PubMed 21539536. Source: BHF-UCL

regulation of cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

response to retinoic acid

Inferred from electronic annotation. Source: Ensembl

stem cell maintenance

Inferred from sequence or structural similarity. Source: UniProtKB

transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentnuclear chromatin

Inferred from direct assay PubMed 21539536. Source: BHF-UCL

nucleus

Inferred from direct assay. Source: HPA

transcription factor complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from mutant phenotype PubMed 21539536. Source: BHF-UCL

RNA polymerase II transcription factor binding

Inferred from physical interaction PubMed 21539536. Source: BHF-UCL

RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 21539536. Source: BHF-UCL

core promoter proximal region sequence-specific DNA binding

Inferred from direct assay PubMed 21539536. Source: BHF-UCL

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

phosphatidylinositol 3-kinase regulator activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 19751731. Source: IntAct

sequence-specific DNA binding transcription factor activity

Non-traceable author statement Ref.1. Source: UniProtKB

sequence-specific DNA binding transcription factor recruiting transcription factor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

transcription regulatory region DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CTBP1Q133634EBI-7232405,EBI-908846

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O43474-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O43474-1)

The sequence of this isoform differs from the canonical sequence as follows:
     367-400: Missing.
Isoform 3 (identifier: O43474-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
     367-400: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: O43474-5)

Also known as: 1a;

The sequence of this isoform differs from the canonical sequence as follows:
     43-118: RWREELSHMK...LSNSLTHPPE → SSCHPVPACQ...VDGNSPAQMN
     119-513: Missing.
Isoform 5 (identifier: O43474-6)

The sequence of this isoform differs from the canonical sequence as follows:
     43-63: RWREELSHMKRLPPVLPGRPY → VRNLTTVTGTAVDGNSPAQMN
     64-513: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513Krueppel-like factor 4
PRO_0000047167

Regions

Zinc finger430 – 45425C2H2-type 1
Zinc finger460 – 48425C2H2-type 2
Zinc finger490 – 51223C2H2-type 3
Region473 – 50432Interaction with target DNA By similarity
Motif101 – 10999aaTAD
Compositional bias125 – 14925Ser-rich
Compositional bias179 – 415237Pro-rich

Amino acid modifications

Modified residue2541Phosphoserine Ref.16
Cross-link32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.14

Natural variations

Alternative sequence1 – 5050Missing in isoform 3.
VSP_040569
Alternative sequence43 – 11876RWREE…THPPE → SSCHPVPACQRSPSQRGEDD RGPGKGPPPTLVITRAAAKP TQRVPISRHTCEPTQVRNLT TVTGTAVDGNSPAQMN in isoform 4.
VSP_047470
Alternative sequence43 – 6321RWREE…PGRPY → VRNLTTVTGTAVDGNSPAQM N in isoform 5.
VSP_047471
Alternative sequence64 – 513450Missing in isoform 5.
VSP_047472
Alternative sequence119 – 513395Missing in isoform 4.
VSP_047473
Alternative sequence367 – 40034Missing in isoform 2 and isoform 3.
VSP_036399
Natural variant3151T → S. Ref.4
Corresponds to variant rs1059913 [ dbSNP | Ensembl ].
VAR_059888
Natural variant3211L → F. Ref.4
Corresponds to variant rs1059914 [ dbSNP | Ensembl ].
VAR_059889

Experimental info

Sequence conflict60 – 612GR → AG in AAC03462. Ref.1
Sequence conflict771G → A in AAC03462. Ref.1
Sequence conflict2511S → T in AAC03462. Ref.1
Sequence conflict3041D → N in AAB48399. Ref.4
Sequence conflict3291D → E in AAB48399. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 10, 2009. Version 3.
Checksum: 3B6113A3EF333935

FASTA51354,671
        10         20         30         40         50         60 
MRQPPGESDM AVSDALLPSF STFASGPAGR EKTLRQAGAP NNRWREELSH MKRLPPVLPG 

        70         80         90        100        110        120 
RPYDLAAATV ATDLESGGAG AACGGSNLAP LPRRETEEFN DLLDLDFILS NSLTHPPESV 

       130        140        150        160        170        180 
AATVSSSASA SSSSSPSSSG PASAPSTCSF TYPIRAGNDP GVAPGGTGGG LLYGRESAPP 

       190        200        210        220        230        240 
PTAPFNLADI NDVSPSGGFV AELLRPELDP VYIPPQQPQP PGGGLMGKFV LKASLSAPGS 

       250        260        270        280        290        300 
EYGSPSVISV SKGSPDGSHP VVVAPYNGGP PRTCPKIKQE AVSSCTHLGA GPPLSNGHRP 

       310        320        330        340        350        360 
AAHDFPLGRQ LPSRTTPTLG LEEVLSSRDC HPALPLPPGF HPHPGPNYPS FLPDQMQPQV 

       370        380        390        400        410        420 
PPLHYQGQSR GFVARAGEPC VCWPHFGTHG MMLTPPSSPL ELMPPGSCMP EEPKPKRGRR 

       430        440        450        460        470        480 
SWPRKRTATH TCDYAGCGKT YTKSSHLKAH LRTHTGEKPY HCDWDGCGWK FARSDELTRH 

       490        500        510 
YRKHTGHRPF QCQKCDRAFS RSDHLALHMK RHF 

« Hide

Isoform 2 [UniParc].

Checksum: 039095BBF3E3F78D
Show »

FASTA47951,076
Isoform 3 [UniParc].

Checksum: 99B86BE95A31F23D
Show »

FASTA42945,639
Isoform 4 (1a) [UniParc].

Checksum: 9E900F0852665920
Show »

FASTA11812,329
Isoform 5 [UniParc].

Checksum: 2A7B5BE789162A89
Show »

FASTA636,489

References

« Hide 'large scale' references
[1]"Human EZF, a Kruppel-like zinc finger protein, is expressed in vascular endothelial cells and contains transcriptional activation and repression domains."
Yet S.-F., McA'Nulty M.M., Folta S.C., Yen H.-W., Yoshizumi M., Hsieh C.-M., Layne M.D., Chin M.T., Wang H., Perrella M.A., Jain M.K., Lee M.-E.
J. Biol. Chem. 273:1026-1031(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Oncogene expression cloning by retroviral transduction of adenovirus E1A-immortalized rat kidney RK3E cells: transformation of a host with epithelial features by c-MYC and the zinc finger protein GKLF."
Foster K.W., Ren S., Louro I.D., Lobo-Ruppert S.M., McKie-Bell P., Grizzle W., Hayes M.R., Broker T.R., Chow L.T., Ruppert J.M.
Cell Growth Differ. 10:423-434(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Shaking the family tree: Identification of novel and biologically active alternatively spliced isoforms across the KLF family of transcription factors."
Camacho-Vanegas O., Till J., Miranda-Lorenzo I., Ozturk B., Camacho S.C., Martignetti J.A.
FASEB J. 27:432-436(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 4 AND 5), ALTERNATIVE SPLICING.
[4]Garrett-Sinha L.A., de Crombrugghe B.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANTS SER-315 AND PHE-321.
Tissue: Placenta.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Substantia nigra and Tongue.
[6]SeattleSNPs variation discovery resource
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Cervix and Lung.
[10]"Human mucin 1 oncoprotein represses transcription of the p53 tumor suppressor gene."
Wei X., Xu H., Kufe D.
Cancer Res. 67:1853-1858(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MUC1, FUNCTION.
[11]"Induction of pluripotent stem cells from adult human fibroblasts by defined factors."
Takahashi K., Tanabe K., Ohnuki M., Narita M., Ichisaka T., Tomoda K., Yamanaka S.
Cell 131:861-872(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY.
[12]"Nine-amino-acid transactivation domain: establishment and prediction utilities."
Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells."
Tan F., Lu L., Cai Y., Wang J., Xie Y., Wang L., Gong Y., Xu B.-E., Wu J., Luo Y., Qiang B., Yuan J., Sun X., Peng X.
Proteomics 8:2885-2896(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-32.
Tissue: Cervix carcinoma.
[15]"Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression."
Zhang P., Andrianakos R., Yang Y., Liu C., Lu W.
J. Biol. Chem. 285:9180-9189(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[16]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[17]"Cooperative transcriptional activation by Klf4, Meis2, and Pbx1."
Bjerke G.A., Hyman-Walsh C., Wotton D.
Mol. Cell. Biol. 31:3723-3733(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PBX1 AND MEIS2.
[18]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF022184 mRNA. Translation: AAC03462.1. Different initiation.
AF105036 mRNA. Translation: AAD42165.1. Different initiation.
HF546201 mRNA. Translation: CCO02787.1.
HF546202 mRNA. Translation: CCO02788.1.
U70663 mRNA. Translation: AAB48399.1. Different initiation.
AK095134 mRNA. Translation: BAG52991.1.
AK313489 mRNA. Translation: BAG36271.1. Different initiation.
DQ658241 Genomic DNA. Translation: ABG25917.1. Sequence problems.
AL360218 Genomic DNA. Translation: CAI12254.2.
CH471105 Genomic DNA. Translation: EAW59020.1. Sequence problems.
CH471105 Genomic DNA. Translation: EAW59021.1. Sequence problems.
BC029923 mRNA. Translation: AAH29923.1. Different initiation.
BC030811 mRNA. Translation: AAH30811.1. Different initiation.
CCDSCCDS6770.2. [O43474-1]
RefSeqNP_004226.3. NM_004235.4. [O43474-1]
XP_005252362.1. XM_005252305.1. [O43474-3]
UniGeneHs.376206.

3D structure databases

ProteinModelPortalO43474.
SMRO43474. Positions 427-513.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114726. 21 interactions.
DIPDIP-57667N.
IntActO43474. 2 interactions.
MINTMINT-7261942.
STRING9606.ENSP00000363804.

PTM databases

PhosphoSiteO43474.

Proteomic databases

MaxQBO43474.
PaxDbO43474.
PRIDEO43474.

Protocols and materials databases

DNASU9314.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000374672; ENSP00000363804; ENSG00000136826. [O43474-1]
GeneID9314.
KEGGhsa:9314.
UCSCuc004bdf.2. human. [O43474-4]
uc004bdg.3. human. [O43474-1]
uc004bdh.3. human. [O43474-3]

Organism-specific databases

CTD9314.
GeneCardsGC09M110247.
HGNCHGNC:6348. KLF4.
HPAHPA002926.
MIM602253. gene.
neXtProtNX_O43474.
PharmGKBPA30138.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOVERGENHBG006220.
KOK17846.
OMACTVGRPL.
PhylomeDBO43474.
TreeFamTF350556.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.
SignaLinkO43474.

Gene expression databases

ArrayExpressO43474.
BgeeO43474.
CleanExHS_KLF4.
GenevestigatorO43474.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKLF4.
GenomeRNAi9314.
NextBio34891.
PROO43474.
SOURCESearch...

Entry information

Entry nameKLF4_HUMAN
AccessionPrimary (citable) accession number: O43474
Secondary accession number(s): B2R8S4 expand/collapse secondary AC list , B3KT79, L0R3I6, L0R4N5, P78338, Q5T3J8, Q5T3J9, Q8N717, Q9UNP3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 10, 2009
Last modified: July 9, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM