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O43422

- P52K_HUMAN

UniProt

O43422 - P52K_HUMAN

Protein

52 kDa repressor of the inhibitor of the protein kinase

Gene

PRKRIR

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (11 Jan 2001)
      Previous versions | rss
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    Functioni

    Upstream regulator of interferon-induced serine/threonine protein kinase R (PKR). May block the PKR-inhibitory function of P58IPK, resulting in restoration of kinase activity and suppression of cell growth.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1 – 8686THAP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. negative regulation of cell proliferation Source: ProtInc
    2. response to stress Source: ProtInc
    3. signal transduction Source: ProtInc

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    52 kDa repressor of the inhibitor of the protein kinase
    Short name:
    p52rIPK
    Alternative name(s):
    58 kDa interferon-induced protein kinase-interacting protein
    Short name:
    p58IPK-interacting protein
    Death-associated protein 4
    THAP domain-containing protein 0
    Gene namesi
    Name:PRKRIR
    Synonyms:DAP4, P52RIPK, THAP0
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:9440. PRKRIR.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: Ensembl

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33781.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 76176152 kDa repressor of the inhibitor of the protein kinasePRO_0000068634Add
    BLAST

    Proteomic databases

    MaxQBiO43422.
    PaxDbiO43422.
    PRIDEiO43422.

    PTM databases

    PhosphoSiteiO43422.

    Expressioni

    Gene expression databases

    ArrayExpressiO43422.
    BgeeiO43422.
    CleanExiHS_PRKRIR.
    GenevestigatoriO43422.

    Organism-specific databases

    HPAiHPA008758.

    Interactioni

    Subunit structurei

    Interacts with P58IPK, probably sequestring it.

    Protein-protein interaction databases

    BioGridi111598. 8 interactions.
    IntActiO43422. 3 interactions.
    MINTiMINT-1531238.
    STRINGi9606.ENSP00000260045.

    Structurei

    3D structure databases

    ProteinModelPortaliO43422.
    SMRiO43422. Positions 1-97.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 THAP-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1 – 8686THAP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG145047.
    HOGENOMiHOG000013127.
    HOVERGENiHBG007908.
    InParanoidiO43422.
    OMAiKFRRAQH.
    PhylomeDBiO43422.
    TreeFamiTF330114.

    Family and domain databases

    InterProiIPR025398. DUF4371.
    IPR008906. HATC_dom_C.
    IPR012337. RNaseH-like_dom.
    IPR006612. Znf_C2CH.
    [Graphical view]
    PfamiPF05699. Dimer_Tnp_hAT. 1 hit.
    PF14291. DUF4371. 1 hit.
    PF05485. THAP. 1 hit.
    [Graphical view]
    SMARTiSM00692. DM3. 1 hit.
    SM00980. THAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 2 hits.
    PROSITEiPS50950. ZF_THAP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: O43422-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPNFCAAPNC TRKSTQSDLA FFRFPRDPAR CQKWVENCRR ADLEDKTPDQ    50
    LNKHYRLCAK HFETSMICRT SPYRTVLRDN AIPTIFDLTS HLNNPHSRHR 100
    KRIKELSEDE IRTLKQKKID ETSEQEQKHK ETNNSNAQNP SEEEGEGQDE 150
    DILPLTLEEK ENKEYLKSLF EILILMGKQN IPLDGHEADE IPEGLFTPDN 200
    FQALLECRIN SGEEVLRKRF ETTAVNTLFC SKTQQRQMLE ICESCIREET 250
    LREVRDSHFF SIITDDVVDI AGEEHLPVLV RFVDESHNLR EEFIGFLPYE 300
    ADAEILAVKF HTMITEKWGL NMEYCRGQAY IVSSGFSSKM KVVASRLLEK 350
    YPQAIYTLCS SCALNMWLAK SVPVMGVSVA LGTIEEVCSF FHRSPQLLLE 400
    LDNVISVLFQ NSKERGKELK EICHSQWTGR HDAFEILVEL LQALVLCLDG 450
    INSDTNIRWN NYIAGRAFVL CSAVSDFDFI VTIVVLKNVL SFTRAFGKNL 500
    QGQTSDVFFA AGSLTAVLHS LNEVMENIEV YHEFWFEEAT NLATKLDIQM 550
    KLPGKFRRAH QGNLESQLTS ESYYKETLSV PTVEHIIQEL KDIFSEQHLK 600
    ALKCLSLVPS VMGQLKFNTS EEHHADMYRS DLPNPDTLSA ELHCWRIKWK 650
    HRGKDIELPS TIYEALHLPD IKFFPNVYAL LKVLCILPVM KVENERYENG 700
    RKRLKAYLRN TLTDQRSSNL ALLNINFDIK HDLDLMVDTY IKLYTSKSEL 750
    PTDNSETVEN T 761
    Length:761
    Mass (Da):87,704
    Last modified:January 11, 2001 - v2
    Checksum:i88809CFE52399C13
    GO
    Isoform Short (identifier: O43422-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         489-492: VLSF → EIKI
         493-761: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:492
    Mass (Da):56,656
    Checksum:iE0CC8E2C5DD93215
    GO

    Sequence cautioni

    The sequence CAB43226.1 differs from that shown. Reason: Frameshift at position 471.
    The sequence AAH21992.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti157 – 1571L → W(PubMed:17974005)Curated
    Sequence conflicti738 – 7381D → V(PubMed:17974005)Curated
    Sequence conflicti754 – 7541N → K(PubMed:17974005)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei489 – 4924VLSF → EIKI in isoform Short. 1 PublicationVSP_004355
    Alternative sequencei493 – 761269Missing in isoform Short. 1 PublicationVSP_004356Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007393 mRNA. Translation: AAC39564.1.
    AF081567 mRNA. Translation: AAG01570.1.
    AK291843 mRNA. Translation: BAF84532.1.
    CH471076 Genomic DNA. Translation: EAW74993.1.
    BC021992 mRNA. Translation: AAH21992.1. Different initiation.
    BC117138 mRNA. Translation: AAI17139.1.
    BC117140 mRNA. Translation: AAI17141.1.
    AL049970 mRNA. Translation: CAB43226.1. Frameshift.
    CCDSiCCDS8243.1. [O43422-1]
    RefSeqiNP_004696.2. NM_004705.2. [O43422-1]
    UniGeneiHs.503315.

    Genome annotation databases

    EnsembliENST00000260045; ENSP00000260045; ENSG00000137492. [O43422-1]
    GeneIDi5612.
    KEGGihsa:5612.
    UCSCiuc001oxh.1. human. [O43422-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007393 mRNA. Translation: AAC39564.1 .
    AF081567 mRNA. Translation: AAG01570.1 .
    AK291843 mRNA. Translation: BAF84532.1 .
    CH471076 Genomic DNA. Translation: EAW74993.1 .
    BC021992 mRNA. Translation: AAH21992.1 . Different initiation.
    BC117138 mRNA. Translation: AAI17139.1 .
    BC117140 mRNA. Translation: AAI17141.1 .
    AL049970 mRNA. Translation: CAB43226.1 . Frameshift.
    CCDSi CCDS8243.1. [O43422-1 ]
    RefSeqi NP_004696.2. NM_004705.2. [O43422-1 ]
    UniGenei Hs.503315.

    3D structure databases

    ProteinModelPortali O43422.
    SMRi O43422. Positions 1-97.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111598. 8 interactions.
    IntActi O43422. 3 interactions.
    MINTi MINT-1531238.
    STRINGi 9606.ENSP00000260045.

    PTM databases

    PhosphoSitei O43422.

    Proteomic databases

    MaxQBi O43422.
    PaxDbi O43422.
    PRIDEi O43422.

    Protocols and materials databases

    DNASUi 5612.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000260045 ; ENSP00000260045 ; ENSG00000137492 . [O43422-1 ]
    GeneIDi 5612.
    KEGGi hsa:5612.
    UCSCi uc001oxh.1. human. [O43422-1 ]

    Organism-specific databases

    CTDi 5612.
    GeneCardsi GC11M076061.
    HGNCi HGNC:9440. PRKRIR.
    HPAi HPA008758.
    MIMi 607374. gene.
    neXtProti NX_O43422.
    PharmGKBi PA33781.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG145047.
    HOGENOMi HOG000013127.
    HOVERGENi HBG007908.
    InParanoidi O43422.
    OMAi KFRRAQH.
    PhylomeDBi O43422.
    TreeFami TF330114.

    Miscellaneous databases

    GeneWikii PRKRIR.
    GenomeRNAii 5612.
    NextBioi 21814.
    PROi O43422.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O43422.
    Bgeei O43422.
    CleanExi HS_PRKRIR.
    Genevestigatori O43422.

    Family and domain databases

    InterProi IPR025398. DUF4371.
    IPR008906. HATC_dom_C.
    IPR012337. RNaseH-like_dom.
    IPR006612. Znf_C2CH.
    [Graphical view ]
    Pfami PF05699. Dimer_Tnp_hAT. 1 hit.
    PF14291. DUF4371. 1 hit.
    PF05485. THAP. 1 hit.
    [Graphical view ]
    SMARTi SM00692. DM3. 1 hit.
    SM00980. THAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 2 hits.
    PROSITEi PS50950. ZF_THAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of interferon-induced protein kinase PKR: modulation of P58IPK inhibitory function by a novel protein, P52rIPK."
      Gale M.J. Jr., Blakely C.M., Hopkins D.A., Melville M.W., Wambach M., Romano P.R., Katze M.G.
      Mol. Cell. Biol. 18:859-871(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    2. Barzilay G., Kimchi A.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Tissue: Brain and Prostate.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-761 (ISOFORM LONG).
      Tissue: Brain.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiP52K_HUMAN
    AccessioniPrimary (citable) accession number: O43422
    Secondary accession number(s): A8K728
    , Q17RY9, Q8WTW1, Q9Y3Z4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3