O43422 (P52K_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
52 kDa repressor of the inhibitor of the protein kinase
Short name=p52rIPK

Alternative name(s):
58 kDa interferon-induced protein kinase-interacting protein
Short name=p58IPK-interacting protein
Death-associated protein 4
THAP domain-containing protein 0

Gene names

Name:	PRKRIR
Synonyms:	DAP4, P52RIPK, THAP0

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Upstream regulator of interferon-induced serine/threonine protein kinase R (PKR). May block the PKR-inhibitory function of P58IPK, resulting in restoration of kinase activity and suppression of cell growth.
Subunit structure	Interacts with P58IPK, probably sequestring it.
Sequence similarities	Contains 1 THAP-type zinc finger.
Sequence caution	The sequence AAH21992.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB43226.1 differs from that shown. Reason: Frameshift at position 471.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc response to stress Traceable author statement Ref.1. Source: ProtInc signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: O43422-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: O43422-2)

The sequence of this isoform differs from the canonical sequence as follows:
     489-492: VLSF → EIKI
     493-761: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 761	761	52 kDa repressor of the inhibitor of the protein kinase		PRO_0000068634
Regions
	Zinc finger	1 – 86	86	THAP-type
Natural variations
	Alternative sequence	489 – 492	4	VLSF → EIKI in isoform Short.		VSP_004355
	Alternative sequence	493 – 761	269	Missing in isoform Short.		VSP_004356
Experimental info
	Sequence conflict	157	1	L → W Ref.6
	Sequence conflict	738	1	D → V Ref.6
	Sequence conflict	754	1	N → K Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 88809CFE52399C13	FASTA	761	87,704
	10 20 30 40 50 60 MPNFCAAPNC TRKSTQSDLA FFRFPRDPAR CQKWVENCRR ADLEDKTPDQ LNKHYRLCAK 70 80 90 100 110 120 HFETSMICRT SPYRTVLRDN AIPTIFDLTS HLNNPHSRHR KRIKELSEDE IRTLKQKKID 130 140 150 160 170 180 ETSEQEQKHK ETNNSNAQNP SEEEGEGQDE DILPLTLEEK ENKEYLKSLF EILILMGKQN 190 200 210 220 230 240 IPLDGHEADE IPEGLFTPDN FQALLECRIN SGEEVLRKRF ETTAVNTLFC SKTQQRQMLE 250 260 270 280 290 300 ICESCIREET LREVRDSHFF SIITDDVVDI AGEEHLPVLV RFVDESHNLR EEFIGFLPYE 310 320 330 340 350 360 ADAEILAVKF HTMITEKWGL NMEYCRGQAY IVSSGFSSKM KVVASRLLEK YPQAIYTLCS 370 380 390 400 410 420 SCALNMWLAK SVPVMGVSVA LGTIEEVCSF FHRSPQLLLE LDNVISVLFQ NSKERGKELK 430 440 450 460 470 480 EICHSQWTGR HDAFEILVEL LQALVLCLDG INSDTNIRWN NYIAGRAFVL CSAVSDFDFI 490 500 510 520 530 540 VTIVVLKNVL SFTRAFGKNL QGQTSDVFFA AGSLTAVLHS LNEVMENIEV YHEFWFEEAT 550 560 570 580 590 600 NLATKLDIQM KLPGKFRRAH QGNLESQLTS ESYYKETLSV PTVEHIIQEL KDIFSEQHLK 610 620 630 640 650 660 ALKCLSLVPS VMGQLKFNTS EEHHADMYRS DLPNPDTLSA ELHCWRIKWK HRGKDIELPS 670 680 690 700 710 720 TIYEALHLPD IKFFPNVYAL LKVLCILPVM KVENERYENG RKRLKAYLRN TLTDQRSSNL 730 740 750 760 ALLNINFDIK HDLDLMVDTY IKLYTSKSEL PTDNSETVEN T
	Isoform Short [UniParc]. Checksum: E0CC8E2C5DD93215	FASTA	492	56,656
	10 20 30 40 50 60 MPNFCAAPNC TRKSTQSDLA FFRFPRDPAR CQKWVENCRR ADLEDKTPDQ LNKHYRLCAK 70 80 90 100 110 120 HFETSMICRT SPYRTVLRDN AIPTIFDLTS HLNNPHSRHR KRIKELSEDE IRTLKQKKID 130 140 150 160 170 180 ETSEQEQKHK ETNNSNAQNP SEEEGEGQDE DILPLTLEEK ENKEYLKSLF EILILMGKQN 190 200 210 220 230 240 IPLDGHEADE IPEGLFTPDN FQALLECRIN SGEEVLRKRF ETTAVNTLFC SKTQQRQMLE 250 260 270 280 290 300 ICESCIREET LREVRDSHFF SIITDDVVDI AGEEHLPVLV RFVDESHNLR EEFIGFLPYE 310 320 330 340 350 360 ADAEILAVKF HTMITEKWGL NMEYCRGQAY IVSSGFSSKM KVVASRLLEK YPQAIYTLCS 370 380 390 400 410 420 SCALNMWLAK SVPVMGVSVA LGTIEEVCSF FHRSPQLLLE LDNVISVLFQ NSKERGKELK 430 440 450 460 470 480 EICHSQWTGR HDAFEILVEL LQALVLCLDG INSDTNIRWN NYIAGRAFVL CSAVSDFDFI 490 VTIVVLKNEI KI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of interferon-induced protein kinase PKR: modulation of P58IPK inhibitory function by a novel protein, P52rIPK." Gale M.J. Jr., Blakely C.M., Hopkins D.A., Melville M.W., Wambach M., Romano P.R., Katze M.G. Mol. Cell. Biol. 18:859-871(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]	Barzilay G., Kimchi A. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Brain and Prostate.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-761 (ISOFORM LONG). Tissue: Brain.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF007393 mRNA. Translation: AAC39564.1. AF081567 mRNA. Translation: AAG01570.1. AK291843 mRNA. Translation: BAF84532.1. CH471076 Genomic DNA. Translation: EAW74993.1. BC021992 mRNA. Translation: AAH21992.1. Different initiation. BC117138 mRNA. Translation: AAI17139.1. BC117140 mRNA. Translation: AAI17141.1. AL049970 mRNA. Translation: CAB43226.1. Frameshift.
IPI	IPI00012438. IPI00219175.
RefSeq	NP_004696.2. NM_004705.2.
UniGene	Hs.503315.
3D structure databases
ProteinModelPortal	O43422.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43422. 1 interaction.
STRING	9606.ENSP00000260045.
PTM databases
PhosphoSite	O43422.
Proteomic databases
PaxDb	O43422.
PRIDE	O43422.
Protocols and materials databases
DNASU	5612.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000260045; ENSP00000260045; ENSG00000137492.
GeneID	5612.
KEGG	hsa:5612.
UCSC	uc001oxh.1. human.
Organism-specific databases
CTD	5612.
GeneCards	GC11M076061.
HGNC	HGNC:9440. PRKRIR.
HPA	HPA008758.
MIM	607374. gene.
neXtProt	NX_O43422.
PharmGKB	PA33781.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG145047.
HOGENOM	HOG000013127.
HOVERGEN	HBG007908.
InParanoid	O43422.
OMA	TNVRWNN.
OrthoDB	EOG4TMR1D.
PhylomeDB	O43422.
Gene expression databases
ArrayExpress	O43422.
Bgee	O43422.
CleanEx	HS_PRKRIR.
Genevestigator	O43422.
GermOnline	ENSG00000137492. Homo sapiens.
Family and domain databases
InterPro	IPR025398. DUF4371. IPR008906. HATC. IPR012337. RNaseH-like_dom. IPR006612. Znf_C2CH. [Graphical view]
Pfam	PF05699. Dimer_Tnp_hAT. 1 hit. PF14291. DUF4371. 1 hit. PF05485. THAP. 1 hit. [Graphical view]
SMART	SM00692. DM3. 1 hit. SM00980. THAP. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS50950. ZF_THAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5612.
NextBio	21814.
SOURCE	Search...

Entry information

Entry name

P52K_HUMAN

Accession

Primary (citable) accession number: O43422
Secondary accession number(s): A8K728 , Q17RY9, Q8WTW1, Q9Y3Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.