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O43422 (P52K_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
52 kDa repressor of the inhibitor of the protein kinase

Short name=p52rIPK
Alternative name(s):
58 kDa interferon-induced protein kinase-interacting protein
Short name=p58IPK-interacting protein
Death-associated protein 4
THAP domain-containing protein 0
Gene names
Name:PRKRIR
Synonyms:DAP4, P52RIPK, THAP0
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length761 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Upstream regulator of interferon-induced serine/threonine protein kinase R (PKR). May block the PKR-inhibitory function of P58IPK, resulting in restoration of kinase activity and suppression of cell growth.

Subunit structure

Interacts with P58IPK, probably sequestring it.

Sequence similarities

Contains 1 THAP-type zinc finger.

Sequence caution

The sequence AAH21992.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAB43226.1 differs from that shown. Reason: Frameshift at position 471.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of cell proliferation

Traceable author statement Ref.1. Source: ProtInc

response to stress

Traceable author statement Ref.1. Source: ProtInc

signal transduction

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentnucleus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: O43422-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: O43422-2)

The sequence of this isoform differs from the canonical sequence as follows:
     489-492: VLSF → EIKI
     493-761: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 76176152 kDa repressor of the inhibitor of the protein kinase
PRO_0000068634

Regions

Zinc finger1 – 8686THAP-type

Natural variations

Alternative sequence489 – 4924VLSF → EIKI in isoform Short.
VSP_004355
Alternative sequence493 – 761269Missing in isoform Short.
VSP_004356

Experimental info

Sequence conflict1571L → W Ref.6
Sequence conflict7381D → V Ref.6
Sequence conflict7541N → K Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 88809CFE52399C13

FASTA76187,704
        10         20         30         40         50         60 
MPNFCAAPNC TRKSTQSDLA FFRFPRDPAR CQKWVENCRR ADLEDKTPDQ LNKHYRLCAK 

        70         80         90        100        110        120 
HFETSMICRT SPYRTVLRDN AIPTIFDLTS HLNNPHSRHR KRIKELSEDE IRTLKQKKID 

       130        140        150        160        170        180 
ETSEQEQKHK ETNNSNAQNP SEEEGEGQDE DILPLTLEEK ENKEYLKSLF EILILMGKQN 

       190        200        210        220        230        240 
IPLDGHEADE IPEGLFTPDN FQALLECRIN SGEEVLRKRF ETTAVNTLFC SKTQQRQMLE 

       250        260        270        280        290        300 
ICESCIREET LREVRDSHFF SIITDDVVDI AGEEHLPVLV RFVDESHNLR EEFIGFLPYE 

       310        320        330        340        350        360 
ADAEILAVKF HTMITEKWGL NMEYCRGQAY IVSSGFSSKM KVVASRLLEK YPQAIYTLCS 

       370        380        390        400        410        420 
SCALNMWLAK SVPVMGVSVA LGTIEEVCSF FHRSPQLLLE LDNVISVLFQ NSKERGKELK 

       430        440        450        460        470        480 
EICHSQWTGR HDAFEILVEL LQALVLCLDG INSDTNIRWN NYIAGRAFVL CSAVSDFDFI 

       490        500        510        520        530        540 
VTIVVLKNVL SFTRAFGKNL QGQTSDVFFA AGSLTAVLHS LNEVMENIEV YHEFWFEEAT 

       550        560        570        580        590        600 
NLATKLDIQM KLPGKFRRAH QGNLESQLTS ESYYKETLSV PTVEHIIQEL KDIFSEQHLK 

       610        620        630        640        650        660 
ALKCLSLVPS VMGQLKFNTS EEHHADMYRS DLPNPDTLSA ELHCWRIKWK HRGKDIELPS 

       670        680        690        700        710        720 
TIYEALHLPD IKFFPNVYAL LKVLCILPVM KVENERYENG RKRLKAYLRN TLTDQRSSNL 

       730        740        750        760 
ALLNINFDIK HDLDLMVDTY IKLYTSKSEL PTDNSETVEN T 

« Hide

Isoform Short [UniParc].

Checksum: E0CC8E2C5DD93215
Show »

FASTA49256,656

References

« Hide 'large scale' references
[1]"Regulation of interferon-induced protein kinase PKR: modulation of P58IPK inhibitory function by a novel protein, P52rIPK."
Gale M.J. Jr., Blakely C.M., Hopkins D.A., Melville M.W., Wambach M., Romano P.R., Katze M.G.
Mol. Cell. Biol. 18:859-871(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]Barzilay G., Kimchi A.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Tissue: Brain and Prostate.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-761 (ISOFORM LONG).
Tissue: Brain.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF007393 mRNA. Translation: AAC39564.1.
AF081567 mRNA. Translation: AAG01570.1.
AK291843 mRNA. Translation: BAF84532.1.
CH471076 Genomic DNA. Translation: EAW74993.1.
BC021992 mRNA. Translation: AAH21992.1. Different initiation.
BC117138 mRNA. Translation: AAI17139.1.
BC117140 mRNA. Translation: AAI17141.1.
AL049970 mRNA. Translation: CAB43226.1. Frameshift.
CCDSCCDS8243.1. [O43422-1]
RefSeqNP_004696.2. NM_004705.2. [O43422-1]
UniGeneHs.503315.

3D structure databases

ProteinModelPortalO43422.
SMRO43422. Positions 1-97.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111598. 7 interactions.
IntActO43422. 3 interactions.
MINTMINT-1531238.
STRING9606.ENSP00000260045.

PTM databases

PhosphoSiteO43422.

Proteomic databases

MaxQBO43422.
PaxDbO43422.
PRIDEO43422.

Protocols and materials databases

DNASU5612.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000260045; ENSP00000260045; ENSG00000137492. [O43422-1]
GeneID5612.
KEGGhsa:5612.
UCSCuc001oxh.1. human. [O43422-1]

Organism-specific databases

CTD5612.
GeneCardsGC11M076061.
HGNCHGNC:9440. PRKRIR.
HPAHPA008758.
MIM607374. gene.
neXtProtNX_O43422.
PharmGKBPA33781.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG145047.
HOGENOMHOG000013127.
HOVERGENHBG007908.
InParanoidO43422.
OMAKFRRAQH.
PhylomeDBO43422.
TreeFamTF330114.

Gene expression databases

ArrayExpressO43422.
BgeeO43422.
CleanExHS_PRKRIR.
GenevestigatorO43422.

Family and domain databases

InterProIPR025398. DUF4371.
IPR008906. HATC_dom_C.
IPR012337. RNaseH-like_dom.
IPR006612. Znf_C2CH.
[Graphical view]
PfamPF05699. Dimer_Tnp_hAT. 1 hit.
PF14291. DUF4371. 1 hit.
PF05485. THAP. 1 hit.
[Graphical view]
SMARTSM00692. DM3. 1 hit.
SM00980. THAP. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 2 hits.
PROSITEPS50950. ZF_THAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPRKRIR.
GenomeRNAi5612.
NextBio21814.
PROO43422.
SOURCESearch...

Entry information

Entry nameP52K_HUMAN
AccessionPrimary (citable) accession number: O43422
Secondary accession number(s): A8K728 expand/collapse secondary AC list , Q17RY9, Q8WTW1, Q9Y3Z4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM