O43414 (ERI3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ERI1 exoribonuclease 3
EC=3.1.-.-

Alternative name(s):
Prion interactor 1
Prion protein-interacting protein

Gene names

Name:	ERI3
Synonyms:	PINT1, PRNPIP, PRNPIP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 2 magnesium ions per subunit By similarity.
Subunit structure	Interacts with PRNP By similarity.
Sequence similarities	Contains 1 exonuclease domain.
Sequence caution	The sequence AAC19158.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43414-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43414-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MATASPAADG...TCDKPQIHPQ → MFVMLQVPWL...MEVLAGQYGM

Isoform 3 (identifier: O43414-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-209: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 337

337

ERI1 exoribonuclease 3

PRO_0000317626

Regions

Domain

146 – 320

175

Exonuclease

Sites

Active site

152

1

Proton acceptor Potential

Active site

307

1

Proton acceptor Potential

Metal binding

150

1

Magnesium 1 By similarity

Metal binding

150

1

Magnesium 2 By similarity

Metal binding

152

1

Magnesium 1 By similarity

Metal binding

249

1

Magnesium 2 By similarity

Metal binding

312

1

Magnesium 1 By similarity

Binding site

152

1

AMP By similarity

Binding site

307

1

AMP By similarity

Natural variations

Alternative sequence

1 – 209

209

Missing in isoform 3.

VSP_031104

Alternative sequence

1 – 163

163

MATAS…QIHPQ → MFVMLQVPWLQSCANLVQIG APLPLLGCLGQYFEVLGSME VLAGQYGM in isoform 2.

VSP_031103

Secondary structure

1

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337

Helix Strand Turn

Details...

Beta strand

144 – 149

6

Beta strand

165 – 174

10

Turn

175 – 177

3

Beta strand

180 – 187

8

Beta strand

191 – 193

3

Helix

198 – 204

7

Helix

208 – 211

4

Helix

217 – 230

14

Turn

231 – 234

4

Beta strand

240 – 247

8

Helix

248 – 251

4

Helix

253 – 261

9

Helix

267 – 269

3

Beta strand

272 – 274

3

Helix

275 – 283

9

Turn

288 – 290

3

Helix

291 – 297

7

Helix

309 – 325

17

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 28E8A691659B4D67	FASTA	337	37,238
	10 20 30 40 50 60 MATASPAADG GRGRPWEGGL VSWPPAPPLT LPWTWMGPSW GQHPGHWGFP ALTEPSASPA 70 80 90 100 110 120 AGLGIFEVRR VLDASGCSML APLQTGAARF SSYLLSRARK VLGSHLFSPC GVPEFCSIST 130 140 150 160 170 180 RKLAAHGFGA SMAAMVSFPP QRYHYFLVLD FEATCDKPQI HPQEIIEFPI LKLNGRTMEI 190 200 210 220 230 240 ESTFHMYVQP VVHPQLTPFC TELTGIIQAM VDGQPSLQQV LERVDEWMAK EGLLDPNVKS 250 260 270 280 290 300 IFVTCGDWDL KVMLPGQCQY LGLPVADYFK QWINLKKAYS FAMGCWPKNG LLDMNKGLSL 310 320 330 QHIGRPHSGI DDCKNIANIM KTLAYRGFIF KQTSKPF
	Isoform 2 [UniParc]. Checksum: 5DF6E20F9B256DC4	FASTA	222	25,009
	10 20 30 40 50 60 MFVMLQVPWL QSCANLVQIG APLPLLGCLG QYFEVLGSME VLAGQYGMEI IEFPILKLNG 70 80 90 100 110 120 RTMEIESTFH MYVQPVVHPQ LTPFCTELTG IIQAMVDGQP SLQQVLERVD EWMAKEGLLD 130 140 150 160 170 180 PNVKSIFVTC GDWDLKVMLP GQCQYLGLPV ADYFKQWINL KKAYSFAMGC WPKNGLLDMN 190 200 210 220 KGLSLQHIGR PHSGIDDCKN IANIMKTLAY RGFIFKQTSK PF
	Isoform 3 [UniParc]. Checksum: 3DB7393DB221D90F	FASTA	128	14,542
	10 20 30 40 50 60 MVDGQPSLQQ VLERVDEWMA KEGLLDPNVK SIFVTCGDWD LKVMLPGQCQ YLGLPVADYF 70 80 90 100 110 120 KQWINLKKAY SFAMGCWPKN GLLDMNKGLS LQHIGRPHSG IDDCKNIANI MKTLAYRGFI FKQTSKPF

References

[1]	Yu W., Sarginson J., Gibbs R.A. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Lung.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF007157 mRNA. Translation: AAC19158.1. Different initiation.
AK290567 mRNA. Translation: BAF83256.1.
AL390776, AL035417 Genomic DNA. Translation: CAI12491.1.
AL390776, AL035417 Genomic DNA. Translation: CAI12494.1.
AL035417, AL390776 Genomic DNA. Translation: CAI23194.1.
AL035417, AL390776 Genomic DNA. Translation: CAI23196.1.
AC004254 Genomic DNA. Translation: AAC04618.1.
BC001072 mRNA. Translation: AAH01072.1.
BC004456 mRNA. Translation: AAH04456.1.

IPI

IPI00020701.
IPI00644349.
IPI00888358.

RefSeq

NP_076971.1. NM_024066.1.

UniGene

Hs.731413.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XRI	X-ray	2.15	A	137-337	[»]

ProteinModelPortal

O43414.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000361331.

PTM databases

PhosphoSite

O43414.

Proteomic databases

PaxDb

O43414.

PRIDE

O43414.

Protocols and materials databases

DNASU

79033.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000372257; ENSP00000361331; ENSG00000117419.
ENST00000372259; ENSP00000361333; ENSG00000117419.

GeneID

79033.

KEGG

hsa:79033.

UCSC

uc001clt.3. human.

Organism-specific databases

CTD

79033.

GeneCards

GC01M044686.

HGNC

HGNC:17276. ERI3.

HPA

HPA027147.

MIM

609917. gene.

neXtProt

NX_O43414.

PharmGKB

PA164719272.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5018.

HOVERGEN

HBG108293.

OrthoDB

EOG4ZKJMJ.

Gene expression databases

ArrayExpress

O43414.

Bgee

O43414.

CleanEx

HS_ERI3.

Genevestigator

O43414.

Family and domain databases

InterPro

IPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]

Pfam

PF00929. RNase_T. 1 hit.
[Graphical view]

SMART

SM00479. EXOIII. 1 hit.
[Graphical view]

SUPFAM

SSF53098. RNaseH_fold. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

O43414.

GenomeRNAi

79033.

NextBio

67747.

SOURCE

Search...

Entry information

Entry name

ERI3_HUMAN

Accession

Primary (citable) accession number: O43414
Secondary accession number(s): B1AK98 , Q5T2T7, Q5T2T9, Q5TG35, Q9BQA0, Q9UEB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.