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O43347

- MSI1H_HUMAN

UniProt

O43347 - MSI1H_HUMAN

Protein

RNA-binding protein Musashi homolog 1

Gene

MSI1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity.By similarity

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. poly(A) RNA binding Source: UniProtKB
    3. poly(U) RNA binding Source: Ensembl
    4. RNA binding Source: ProtInc

    GO - Biological processi

    1. epithelial cell differentiation Source: Ensembl
    2. nervous system development Source: ProtInc
    3. response to hormone Source: Ensembl

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-binding protein Musashi homolog 1
    Short name:
    Musashi-1
    Gene namesi
    Name:MSI1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:7330. MSI1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. polysome Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31137.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 362362RNA-binding protein Musashi homolog 1PRO_0000081649Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications
    Modified residuei191 – 1911Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO43347.
    PaxDbiO43347.
    PRIDEiO43347.

    PTM databases

    PhosphoSiteiO43347.

    Expressioni

    Tissue specificityi

    Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.2 Publications

    Gene expression databases

    ArrayExpressiO43347.
    BgeeiO43347.
    CleanExiHS_MSI1.
    GenevestigatoriO43347.

    Organism-specific databases

    HPAiCAB022337.

    Interactioni

    Protein-protein interaction databases

    BioGridi110577. 4 interactions.
    IntActiO43347. 1 interaction.
    MINTiMINT-1432501.
    STRINGi9606.ENSP00000257552.

    Structurei

    3D structure databases

    ProteinModelPortaliO43347.
    SMRiO43347. Positions 18-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 11091RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini109 – 18678RRM 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi274 – 2818Poly-Ala

    Domaini

    The first RNA recognition motif binds more strongly to RNA compared to the second one.By similarity

    Sequence similaritiesi

    Belongs to the Musashi family.Curated
    Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0724.
    HOGENOMiHOG000234441.
    HOVERGENiHBG002295.
    InParanoidiO43347.
    KOiK14411.
    OMAiMETDAPQ.
    OrthoDBiEOG715Q6V.
    PhylomeDBiO43347.
    TreeFamiTF325419.

    Family and domain databases

    Gene3Di3.30.70.330. 2 hits.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 2 hits.
    [Graphical view]
    SMARTiSM00360. RRM. 2 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O43347-1 [UniParc]FASTAAdd to Basket

    « Hide

    METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGEVKECL    50
    VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDSKTI DPKVAFPRRA 100
    QPKMVTRTKK IFVGGLSVNT TVEDVKQYFE QFGKVDDAML MFDKTTNRHR 150
    GFGFVTFESE DIVEKVCEIH FHEINNKMVE CKKAQPKEVM SPTGSARGRS 200
    RVMPYGMDAF MLGIGMLGYP GFQATTYASR SYTGLAPGYT YQFPEFRVER 250
    TPLPSAPVLP ELTAIPLTAY GPMAAAAAAA AVVRGTGSHP WTMAPPPGST 300
    PSRTGGFLGT TSPGPMAELY GAANQDSGVS SYISAASPAP STGFGHSLGG 350
    PLIATAFTNG YH 362
    Length:362
    Mass (Da):39,125
    Last modified:June 1, 1998 - v1
    Checksum:i26487B0A32CF1F40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti197 – 1971R → W in BAB70469. (PubMed:12054577)Curated
    Sequence conflicti225 – 2251T → S in BAB69769. (PubMed:12054577)Curated
    Sequence conflicti232 – 2321Y → C in BAB69769. (PubMed:12054577)Curated
    Sequence conflicti236 – 2361A → P in BAB69767. (PubMed:12054577)Curated
    Sequence conflicti245 – 2451E → K in BAB70469. (PubMed:12054577)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti160 – 1601E → Q in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035485

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012851 mRNA. Translation: BAA33962.1.
    AC003982 Genomic DNA. Translation: AAB95636.1.
    AB072590 mRNA. Translation: BAB69767.1.
    AB072591 mRNA. Translation: BAB69768.1.
    AB072592 mRNA. Translation: BAB69769.1.
    AB073212 mRNA. Translation: BAB70469.1.
    CCDSiCCDS9196.1.
    RefSeqiNP_002433.1. NM_002442.3.
    UniGeneiHs.158311.

    Genome annotation databases

    EnsembliENST00000257552; ENSP00000257552; ENSG00000135097.
    GeneIDi4440.
    KEGGihsa:4440.
    UCSCiuc001tye.2. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB012851 mRNA. Translation: BAA33962.1 .
    AC003982 Genomic DNA. Translation: AAB95636.1 .
    AB072590 mRNA. Translation: BAB69767.1 .
    AB072591 mRNA. Translation: BAB69768.1 .
    AB072592 mRNA. Translation: BAB69769.1 .
    AB073212 mRNA. Translation: BAB70469.1 .
    CCDSi CCDS9196.1.
    RefSeqi NP_002433.1. NM_002442.3.
    UniGenei Hs.158311.

    3D structure databases

    ProteinModelPortali O43347.
    SMRi O43347. Positions 18-187.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110577. 4 interactions.
    IntActi O43347. 1 interaction.
    MINTi MINT-1432501.
    STRINGi 9606.ENSP00000257552.

    PTM databases

    PhosphoSitei O43347.

    Proteomic databases

    MaxQBi O43347.
    PaxDbi O43347.
    PRIDEi O43347.

    Protocols and materials databases

    DNASUi 4440.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000257552 ; ENSP00000257552 ; ENSG00000135097 .
    GeneIDi 4440.
    KEGGi hsa:4440.
    UCSCi uc001tye.2. human.

    Organism-specific databases

    CTDi 4440.
    GeneCardsi GC12M120779.
    HGNCi HGNC:7330. MSI1.
    HPAi CAB022337.
    MIMi 603328. gene.
    neXtProti NX_O43347.
    PharmGKBi PA31137.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0724.
    HOGENOMi HOG000234441.
    HOVERGENi HBG002295.
    InParanoidi O43347.
    KOi K14411.
    OMAi METDAPQ.
    OrthoDBi EOG715Q6V.
    PhylomeDBi O43347.
    TreeFami TF325419.

    Miscellaneous databases

    GeneWikii MSI1.
    GenomeRNAii 4440.
    NextBioi 17313.
    PROi O43347.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O43347.
    Bgeei O43347.
    CleanExi HS_MSI1.
    Genevestigatori O43347.

    Family and domain databases

    Gene3Di 3.30.70.330. 2 hits.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 2 hits.
    [Graphical view ]
    SMARTi SM00360. RRM. 2 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells."
      Good P., Yoda A., Sakakibara S., Yamamoto A., Imai T., Sawa H., Ikeuchi T., Tsuji S., Satoh H., Okano H.
      Genomics 52:382-384(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
      Tissue: Fetal brain.
    2. "The finished DNA sequence of human chromosome 12."
      Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
      , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
      Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines."
      Shu H.-J., Saito T., Watanabe H., Ito J., Takeda H., Okano H., Kawata S.
      Biochem. Biophys. Res. Commun. 293:150-154(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 64-245, TISSUE SPECIFICITY.
    4. "Musashi: a translational regulator of cell fate."
      Okano H., Imai T., Okabe M.
      J. Cell Sci. 115:1355-1359(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-160.

    Entry informationi

    Entry nameiMSI1H_HUMAN
    AccessioniPrimary (citable) accession number: O43347
    Secondary accession number(s): Q96PU0
    , Q96PU1, Q96PU2, Q96PU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3