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Protein

RNA-binding protein Musashi homolog 1

Gene

MSI1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).By similarity

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. poly(A) RNA binding Source: UniProtKB
  3. poly(U) RNA binding Source: Ensembl
  4. RNA binding Source: ProtInc

GO - Biological processi

  1. epithelial cell differentiation Source: Ensembl
  2. nervous system development Source: ProtInc
  3. response to hormone Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein Musashi homolog 1
Short name:
Musashi-1
Gene namesi
Name:MSI1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:7330. MSI1.

Subcellular locationi

  1. Cytoplasm By similarity
  2. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. polysome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31137.

Polymorphism and mutation databases

BioMutaiMSI1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362RNA-binding protein Musashi homolog 1PRO_0000081649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications
Modified residuei191 – 1911Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO43347.
PaxDbiO43347.
PRIDEiO43347.

PTM databases

PhosphoSiteiO43347.

Expressioni

Tissue specificityi

Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.2 Publications

Gene expression databases

BgeeiO43347.
CleanExiHS_MSI1.
ExpressionAtlasiO43347. baseline and differential.
GenevestigatoriO43347.

Organism-specific databases

HPAiCAB022337.

Interactioni

Protein-protein interaction databases

BioGridi110577. 5 interactions.
IntActiO43347. 1 interaction.
MINTiMINT-1432501.
STRINGi9606.ENSP00000257552.

Structurei

3D structure databases

ProteinModelPortaliO43347.
SMRiO43347. Positions 18-187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 11091RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini109 – 18678RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi274 – 2818Poly-Ala

Domaini

The first RNA recognition motif binds more strongly to RNA compared to the second one.By similarity

Sequence similaritiesi

Belongs to the Musashi family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000110584.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiO43347.
KOiK14411.
OMAiMETDAPQ.
OrthoDBiEOG715Q6V.
PhylomeDBiO43347.
TreeFamiTF325419.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGEVKECL
60 70 80 90 100
VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDSKTI DPKVAFPRRA
110 120 130 140 150
QPKMVTRTKK IFVGGLSVNT TVEDVKQYFE QFGKVDDAML MFDKTTNRHR
160 170 180 190 200
GFGFVTFESE DIVEKVCEIH FHEINNKMVE CKKAQPKEVM SPTGSARGRS
210 220 230 240 250
RVMPYGMDAF MLGIGMLGYP GFQATTYASR SYTGLAPGYT YQFPEFRVER
260 270 280 290 300
TPLPSAPVLP ELTAIPLTAY GPMAAAAAAA AVVRGTGSHP WTMAPPPGST
310 320 330 340 350
PSRTGGFLGT TSPGPMAELY GAANQDSGVS SYISAASPAP STGFGHSLGG
360
PLIATAFTNG YH
Length:362
Mass (Da):39,125
Last modified:June 1, 1998 - v1
Checksum:i26487B0A32CF1F40
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971R → W in BAB70469 (PubMed:12054577).Curated
Sequence conflicti225 – 2251T → S in BAB69769 (PubMed:12054577).Curated
Sequence conflicti232 – 2321Y → C in BAB69769 (PubMed:12054577).Curated
Sequence conflicti236 – 2361A → P in BAB69767 (PubMed:12054577).Curated
Sequence conflicti245 – 2451E → K in BAB70469 (PubMed:12054577).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti160 – 1601E → Q in a breast cancer sample; somatic mutation. 1 Publication
VAR_035485

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012851 mRNA. Translation: BAA33962.1.
AC003982 Genomic DNA. Translation: AAB95636.1.
AB072590 mRNA. Translation: BAB69767.1.
AB072591 mRNA. Translation: BAB69768.1.
AB072592 mRNA. Translation: BAB69769.1.
AB073212 mRNA. Translation: BAB70469.1.
CCDSiCCDS9196.1.
RefSeqiNP_002433.1. NM_002442.3.
UniGeneiHs.158311.

Genome annotation databases

EnsembliENST00000257552; ENSP00000257552; ENSG00000135097.
GeneIDi4440.
KEGGihsa:4440.
UCSCiuc001tye.2. human.

Polymorphism and mutation databases

BioMutaiMSI1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012851 mRNA. Translation: BAA33962.1.
AC003982 Genomic DNA. Translation: AAB95636.1.
AB072590 mRNA. Translation: BAB69767.1.
AB072591 mRNA. Translation: BAB69768.1.
AB072592 mRNA. Translation: BAB69769.1.
AB073212 mRNA. Translation: BAB70469.1.
CCDSiCCDS9196.1.
RefSeqiNP_002433.1. NM_002442.3.
UniGeneiHs.158311.

3D structure databases

ProteinModelPortaliO43347.
SMRiO43347. Positions 18-187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110577. 5 interactions.
IntActiO43347. 1 interaction.
MINTiMINT-1432501.
STRINGi9606.ENSP00000257552.

PTM databases

PhosphoSiteiO43347.

Polymorphism and mutation databases

BioMutaiMSI1.

Proteomic databases

MaxQBiO43347.
PaxDbiO43347.
PRIDEiO43347.

Protocols and materials databases

DNASUi4440.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257552; ENSP00000257552; ENSG00000135097.
GeneIDi4440.
KEGGihsa:4440.
UCSCiuc001tye.2. human.

Organism-specific databases

CTDi4440.
GeneCardsiGC12M120779.
HGNCiHGNC:7330. MSI1.
HPAiCAB022337.
MIMi603328. gene.
neXtProtiNX_O43347.
PharmGKBiPA31137.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000110584.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiO43347.
KOiK14411.
OMAiMETDAPQ.
OrthoDBiEOG715Q6V.
PhylomeDBiO43347.
TreeFamiTF325419.

Miscellaneous databases

GeneWikiiMSI1.
GenomeRNAii4440.
NextBioi17313.
PROiO43347.
SOURCEiSearch...

Gene expression databases

BgeeiO43347.
CleanExiHS_MSI1.
ExpressionAtlasiO43347. baseline and differential.
GenevestigatoriO43347.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells."
    Good P., Yoda A., Sakakibara S., Yamamoto A., Imai T., Sawa H., Ikeuchi T., Tsuji S., Satoh H., Okano H.
    Genomics 52:382-384(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
    Tissue: Fetal brain.
  2. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines."
    Shu H.-J., Saito T., Watanabe H., Ito J., Takeda H., Okano H., Kawata S.
    Biochem. Biophys. Res. Commun. 293:150-154(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 64-245, TISSUE SPECIFICITY.
  4. "Musashi: a translational regulator of cell fate."
    Okano H., Imai T., Okabe M.
    J. Cell Sci. 115:1355-1359(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-160.

Entry informationi

Entry nameiMSI1H_HUMAN
AccessioniPrimary (citable) accession number: O43347
Secondary accession number(s): Q96PU0
, Q96PU1, Q96PU2, Q96PU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 1998
Last modified: April 29, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.