Reviewed,
UniProtKB/Swiss-Prot O43324 (MCA3_HUMAN)
Last modified
November 25, 2008.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Eukaryotic translation elongation factor 1 epsilon-1 Alternative name(s): Multisynthetase complex auxiliary component p18 Elongation factor p18 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 174 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Positive modulator of ATM response to DNA damage. |
| Subunit structure | Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with ATM and ATR. The interaction with ATM, which takes place independently of TP53, is induced by DNA damage that may occur during genotoxic stress or cell growth. The interaction with ATR is enhanced by UV irradiation. |
| Subcellular location | Cytoplasm. Nucleus. Note= Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage. |
| Tissue specificity | Down-regulated in various cancer tissues. |
| Induction | By DNA damaging agents, such as UV, adriamycin, actinomycin D and cisplatin. |
| Sequence similarities | Contains 1 GST C-terminal domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Protein biosynthesis |
| Cellular component | Cytoplasm Nucleus |
| PTM | Acetylation |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | negative regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of DNA damage response, signal transduction by p53 class mediatorInferred from sequence or structural similarity. Source: UniProtKB positive regulation of apoptosisInferred from sequence or structural similarity. Source: UniProtKB translationInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleusInferred from sequence or structural similarity. Source: UniProtKB |
| Molecular function | protein binding Inferred from sequence or structural similarity. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||||||||||||||||||||||||||||
| Chain | 2 – 174 | 173 | Eukaryotic translation elongation factor 1 epsilon-1 | PRO_0000221132 | |||||||||||||||||||||||||||||||
Regions | |||||||||||||||||||||||||||||||||||
| Domain | 50 – 173 | 124 | GST C-terminal | ||||||||||||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||||||||||||
| Modified residue | 2 | 1 | N-acetylalanine | ||||||||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||||||||
| Helix | 2 – 13 | 12 | |||||||||||||||||||||||||||||||||
| Beta strand | 23 – 25 | 3 | |||||||||||||||||||||||||||||||||
| Turn | 26 – 29 | 4 | |||||||||||||||||||||||||||||||||
| Beta strand | 30 – 34 | 5 | |||||||||||||||||||||||||||||||||
| Beta strand | 40 – 43 | 4 | |||||||||||||||||||||||||||||||||
| Helix | 44 – 54 | 11 | |||||||||||||||||||||||||||||||||
| Helix | 58 – 61 | 4 | |||||||||||||||||||||||||||||||||
| Helix | 65 – 81 | 17 | |||||||||||||||||||||||||||||||||
| Helix | 90 – 101 | 12 | |||||||||||||||||||||||||||||||||
| Helix | 102 – 104 | 3 | |||||||||||||||||||||||||||||||||
| Beta strand | 110 – 112 | 3 | |||||||||||||||||||||||||||||||||
| Helix | 115 – 128 | 14 | |||||||||||||||||||||||||||||||||
| Helix | 133 – 138 | 6 | |||||||||||||||||||||||||||||||||
| Helix | 140 – 151 | 12 | |||||||||||||||||||||||||||||||||
| Turn | 153 – 155 | 3 | |||||||||||||||||||||||||||||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Cloning of cDNA for human p18 from human testis." Motegi H., Noda T., Shiba K. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis. |
| [2] | "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning." Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed: 9653160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Urinary bladder. |
| [6] | Bienvenut W.V., Calvo F., Kolch W. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [7] | "The haploinsufficient tumor suppressor p18 upregulates p53 via interactions with ATM/ATR." Park B.-J., Kang J.W., Lee S.W., Choi S.-J., Shin Y.K., Ahn Y.H., Choi Y.H., Choi D., Lee K.S., Kim S. Cell 120:209-221(2005) [PubMed: 15680327] [Abstract] Cited for: INTERACTION WITH ATM, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AB011079 mRNA. Translation: BAA24926.1. AF054186 mRNA. Translation: AAC39916.1. BT007306 mRNA. Translation: AAP35970.1. AL355499, AL023694, AL451187 Genomic DNA. Translation: CAH72539.1. AL023694, AL355499, AL451187 Genomic DNA. Translation: CAI21644.1. AL451187, AL023694, AL355499 Genomic DNA. Translation: CAI14747.1. BC005291 mRNA. Translation: AAH05291.1. | |||||||||||||
| RefSeq | NP_004271.1. | ||||||||||||
| UniGene | Hs.631818 | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | O43324. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | O43324. | ||||||||||||
Proteomic databases | |||||||||||||
| PeptideAtlas | O43324. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000124802. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 9521. | ||||||||||||
| KEGG | hsa:9521. | ||||||||||||
Organism-specific databases | |||||||||||||
| H-InvDB | HIX0005569. | ||||||||||||
| HGNC | HGNC:3212. EEF1E1. | ||||||||||||
| MIM | 609206. gene. | ||||||||||||
| PharmGKB | PA27648. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
| GeneCards | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | O43324. | ||||||||||||
| HOVERGEN | O43324. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | O43324. | ||||||||||||
| CleanEx | HS_EEF1E1. | ||||||||||||
| GermOnline | ENSG00000124802. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR010987. Glutathione-S-Trfase_C-like. IPR004046. GST_C. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:1.20.1050.10. GST_C_like. 1 hit. | ||||||||||||
| Pfam | PF00043. GST_C. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS50405. GST_CTER. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| LinkHub | O43324. | ||||||||||||
| NextBio | 35684. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | MCA3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O43324 Secondary accession number(s): Q5THS2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


