Reviewed, UniProtKB/Swiss-Prot O43324 (MCA3_HUMAN)

Names and origin

Protein names

Recommended name:
    Eukaryotic translation elongation factor 1 epsilon-1
Alternative name(s):
    Multisynthetase complex auxiliary component p18
    Elongation factor p18

Gene names

Name:	EEF1E1
Synonyms:	P18

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Positive modulator of ATM response to DNA damage.
Subunit structure	Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with ATM and ATR. The interaction with ATM, which takes place independently of TP53, is induced by DNA damage that may occur during genotoxic stress or cell growth. The interaction with ATR is enhanced by UV irradiation.
Subcellular location	Cytoplasm. Nucleus. Note= Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage.
Tissue specificity	Down-regulated in various cancer tissues.
Induction	By DNA damaging agents, such as UV, adriamycin, actinomycin D and cisplatin.
Sequence similarities	Contains 1 GST C-terminal domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm Nucleus
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of DNA damage response, signal transduction by p53 class mediator Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of apoptosis Inferred from sequence or structural similarity. Source: UniProtKB translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 174

173

Eukaryotic translation elongation factor 1 epsilon-1

PRO_0000221132

Regions

Domain

50 – 173

124

GST C-terminal

Amino acid modifications

Modified residue

2

1

N-acetylalanine

Secondary structure

1

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174

Helix Strand Turn

Details...

Helix

2 – 13

12

Beta strand

23 – 25

3

Turn

26 – 29

4

Beta strand

30 – 34

5

Beta strand

40 – 43

4

Helix

44 – 54

11

Helix

58 – 61

4

Helix

65 – 81

17

Helix

90 – 101

12

Helix

102 – 104

3

Beta strand

110 – 112

3

Helix

115 – 128

14

Helix

133 – 138

6

Helix

140 – 151

12

Turn

153 – 155

3

Sequences

	Sequence		Length	Mass (Da)
	O43324-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 58AAE4BD9E1684E2	FASTA	174	19,811
	10 20 30 40 50 60 MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL 70 80 90 100 110 120 LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL 130 140 150 160 170 YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNA for human p18 from human testis." Motegi H., Noda T., Shiba K. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning." Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed: 9653160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Urinary bladder.
[6]	Bienvenut W.V., Calvo F., Kolch W. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"The haploinsufficient tumor suppressor p18 upregulates p53 via interactions with ATM/ATR." Park B.-J., Kang J.W., Lee S.W., Choi S.-J., Shin Y.K., Ahn Y.H., Choi Y.H., Choi D., Lee K.S., Kim S. Cell 120:209-221(2005) [PubMed: 15680327] [Abstract] Cited for: INTERACTION WITH ATM, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB011079 mRNA. Translation: BAA24926.1.
AF054186 mRNA. Translation: AAC39916.1.
BT007306 mRNA. Translation: AAP35970.1.
AL355499, AL023694, AL451187 Genomic DNA. Translation: CAH72539.1.
AL023694, AL355499, AL451187 Genomic DNA. Translation: CAI21644.1.
AL451187, AL023694, AL355499 Genomic DNA. Translation: CAI14747.1.
BC005291 mRNA. Translation: AAH05291.1.

RefSeq

NP_004271.1.

UniGene

Hs.631818

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2UZ8	X-ray	2.00	A/B	1-174	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O43324.

PTM databases

PhosphoSite

O43324.

Proteomic databases

PeptideAtlas

O43324.

Genome annotation databases

Ensembl

ENSG00000124802. Homo sapiens. [Contig view]

GeneID

9521.

KEGG

hsa:9521.

Organism-specific databases

H-InvDB

HIX0005569.

HGNC

HGNC:3212. EEF1E1.

MIM

609206. gene.

PharmGKB

PA27648.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

O43324.

HOVERGEN

O43324.

Gene expression databases

ArrayExpress

O43324.

CleanEx

HS_EEF1E1.

GermOnline

ENSG00000124802. Homo sapiens.

Family and domain databases

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
[Graphical view]

Gene3D

G3DSA:1.20.1050.10. GST_C_like. 1 hit.

Pfam

PF00043. GST_C. 1 hit.
[Graphical view]

PROSITE

PS50405. GST_CTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

O43324.

NextBio

35684.

SOURCE

Search...

Entry information

Entry name

MCA3_HUMAN

Accession

Primary (citable) accession number: O43324
Secondary accession number(s): Q5THS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	June 1, 1998
Last modified:	November 25, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)