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O43312 (MTSS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metastasis suppressor protein 1
Alternative name(s):
Metastasis suppressor YGL-1
Missing in metastasis protein
Gene names
Name:MTSS1
Synonyms:KIAA0429, MIM
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length755 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be related to cancer progression or tumor metastasis in a variety of organ sites, most likely through an interaction with the actin cytoskeleton.

Subunit structure

Binds to actin. Binds to the cytoplasmic domain of receptor protein tyrosine phosphatase delta. Ref.6

Subcellular location

Cytoplasmcytoskeleton.

Tissue specificity

Expressed in many tissues, including spleen, thymus, prostate, testis, uterus, colon, and peripheral blood. Ref.5

Domain

The WH2 motif at the C-terminus binds to actin monomers. Ref.6

Sequence similarities

Belongs to the MTSS1 family.

Contains 1 IMD (IRSp53/MIM homology) domain.

Contains 1 WH2 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
Polymorphism
   DiseaseTumor suppressor
   DomainCoiled coil
   LigandActin-binding
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Traceable author statement Ref.5. Source: UniProtKB

actin filament polymerization

Inferred from electronic annotation. Source: Ensembl

cell adhesion

Non-traceable author statement Ref.6. Source: UniProtKB

cellular component movement

Non-traceable author statement Ref.6. Source: UniProtKB

cellular response to fluid shear stress

Inferred from sequence or structural similarity. Source: UniProtKB

epithelial cell proliferation involved in renal tubule morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

filopodium assembly

Inferred from electronic annotation. Source: InterPro

glomerulus morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

microspike assembly

Non-traceable author statement Ref.6. Source: UniProtKB

negative regulation of epithelial cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

nephron tubule epithelial cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

renal tubule morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

transmembrane receptor protein tyrosine kinase signaling pathway

Traceable author statement Ref.6. Source: UniProtKB

   Cellular_componentactin cytoskeleton

Inferred from direct assay Ref.6. Source: UniProtKB

cytoplasm

Traceable author statement Ref.6. Source: UniProtKB

endocytic vesicle

Traceable author statement Ref.6. Source: UniProtKB

ruffle

Non-traceable author statement Ref.6. Source: UniProtKB

   Molecular_functionactin monomer binding

Inferred from direct assay Ref.6. Source: UniProtKB

identical protein binding

Inferred from physical interaction PubMed 14752106. Source: IntAct

receptor binding

Inferred from physical interaction Ref.6. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-473954,EBI-473954
CttnQ605982EBI-473954,EBI-397955From a different organism.
HTTP428583EBI-473954,EBI-466029

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O43312-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O43312-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-200: Missing.
     345-426: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: O43312-4)

The sequence of this isoform differs from the canonical sequence as follows:
     346-409: LSNGFSHYSL...MFPSSQIPSW → NSSSSASSEA...STMGAWVSTE
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 755755Metastasis suppressor protein 1
PRO_0000096639

Regions

Domain1 – 250250IMD
Domain727 – 74418WH2
Coiled coil108 – 15548 Potential
Compositional bias238 – 359122Ser-rich
Compositional bias608 – 726119Pro-rich

Amino acid modifications

Modified residue3221Phosphoserine By similarity

Natural variations

Alternative sequence1 – 200200Missing in isoform 2.
VSP_007420
Alternative sequence345 – 42682Missing in isoform 2.
VSP_007421
Alternative sequence346 – 40964LSNGF…QIPSW → NSSSSASSEASETCQSVSEC SSPTSVSSGSTMGAWVSTE in isoform 3.
VSP_016216
Natural variant3051N → I.
Corresponds to variant rs2303956 [ dbSNP | Ensembl ].
VAR_054010
Natural variant7251T → A.
Corresponds to variant rs3829037 [ dbSNP | Ensembl ].
VAR_054011

Experimental info

Sequence conflict1531K → KVDTL in AK027015. Ref.2
Sequence conflict5861V → A in AK027015. Ref.2
Sequence conflict6301L → M in AAF15947. Ref.1

Secondary structure

... 755
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 16, 2003. Version 2.
Checksum: 76FEE3224CBA9287

FASTA75582,251
        10         20         30         40         50         60 
MEAVIEKECS ALGGLFQTII SDMKGSYPVW EDFINKAGKL QSQLRTTVVA AAAFLDAFQK 

        70         80         90        100        110        120 
VADMATNTRG GTREIGSALT RMCMRHRSIE AKLRQFSSAL IDCLINPLQE QMEEWKKVAN 

       130        140        150        160        170        180 
QLDKDHAKEY KKARQEIKKK SSDTLKLQKK AKKGRGDIQP QLDSALQDVN DKYLLLEETE 

       190        200        210        220        230        240 
KQAVRKALIE ERGRFCTFIS MLRPVIEEEI SMLGEITHLQ TISEDLKSLT MDPHKLPSSS 

       250        260        270        280        290        300 
EQVILDLKGS DYSWSYQTPP SSPSTTMSRK SSVCSSLNSV NSSDSRSSGS HSHSPSSHYR 

       310        320        330        340        350        360 
YRSSNLAQQA PVRLSSVSSH DSGFISQDAF QSKSPSPMPP EAPNQLSNGF SHYSLSSESH 

       370        380        390        400        410        420 
VGPTGAGLFP HCLPASRLLP RVTSVHLPDY AHYYTIGPGM FPSSQIPSWK DWAKPGPYDQ 

       430        440        450        460        470        480 
PLVNTLQRRK EKREPDPNGG GPTTASGPPA AAEEAQRPRS MTVSAATRPG EEMEACEELA 

       490        500        510        520        530        540 
LALSRGLQLD TQRSSRDSLQ CSSGYSTQTT TPCCSEDTIP SQVSDYDYFS VSGDQEADQQ 

       550        560        570        580        590        600 
EFDKSSTIPR NSDISQSYRR MFQAKRPAST AGLPTTLGPA MVTPGVATIR RTPSTKPSVR 

       610        620        630        640        650        660 
RGTIGAGPIP IKTPVIPVKT PTVPDLPGVL PAPPDGPEER GEHSPESPSV GEGPQGVTSM 

       670        680        690        700        710        720 
PSSMWSGQAS VNPPLPGPKP SIPEEHRQAI PESEAEDQER EPPSATVSPG QIPESDPADL 

       730        740        750 
SPRDTPQGED MLNAIRRGVK LKKTTTNDRS APRFS 

« Hide

Isoform 2 [UniParc].

Checksum: BD0E62304BD0C447
Show »

FASTA47350,650
Isoform 3 [UniParc].

Checksum: FB45648CFA440449
Show »

FASTA73079,144

References

« Hide 'large scale' references
[1]"Identification of potential metastasis suppressor gene (YGL-1) in bladder cancer."
Lee Y.-G., Macoska J.A., Schwab E.D., Korenchuk S., Pienta K.J.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[4]"Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 65-755 (ISOFORM 3).
Tissue: Brain.
[5]"MIM, a potential metastasis suppressor gene in bladder cancer."
Lee Y.-G., Macoska J.A., Korenchuk S., Pienta K.J.
Neoplasia 4:291-294(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 400-755 (ISOFORM 1), TISSUE SPECIFICITY.
[6]"MIM-B, a putative metastasis suppressor protein, binds to actin and to protein tyrosine phosphatase delta."
Woodings J.A., Sharp S.J., Machesky L.M.
Biochem. J. 371:463-471(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION OF WH2 DOMAIN WITH ACTIN, SUBUNIT.
[7]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF086645 mRNA. Translation: AAF15947.1.
AK027015 mRNA. No translation available.
BC023998 mRNA. Translation: AAH23998.1.
AB007889 mRNA. Translation: BAA24859.3.
RefSeqNP_001269903.1. NM_001282974.1.
NP_055566.3. NM_014751.5.
UniGeneHs.336994.
Hs.700429.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2D1KX-ray2.50C724-755[»]
ProteinModelPortalO43312.
SMRO43312. Positions 1-242, 725-753.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115132. 1 interaction.
DIPDIP-17022N.
IntActO43312. 5 interactions.
MINTMINT-3978135.
STRING9606.ENSP00000322804.

PTM databases

PhosphoSiteO43312.

Proteomic databases

PaxDbO43312.
PRIDEO43312.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000354184; ENSP00000346119; ENSG00000170873. [O43312-2]
ENST00000378017; ENSP00000367256; ENSG00000170873. [O43312-4]
ENST00000431961; ENSP00000393606; ENSG00000170873. [O43312-2]
ENST00000518547; ENSP00000429064; ENSG00000170873. [O43312-1]
GeneID9788.
KEGGhsa:9788.
UCSCuc003yri.2. human. [O43312-2]
uc003yrj.2. human. [O43312-4]
uc003yrk.2. human. [O43312-1]

Organism-specific databases

CTD9788.
GeneCardsGC08M125563.
HGNCHGNC:20443. MTSS1.
MIM608486. gene.
neXtProtNX_O43312.
PharmGKBPA134892011.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG72831.
HOGENOMHOG000113691.
HOVERGENHBG052530.
InParanoidO43312.
OrthoDBEOG7T7GSJ.
PhylomeDBO43312.
TreeFamTF320619.

Enzyme and pathway databases

SignaLinkO43312.

Gene expression databases

ArrayExpressO43312.
BgeeO43312.
CleanExHS_MTSS1.
GenevestigatorO43312.

Family and domain databases

InterProIPR013606. IRSp53/MIM_homology_IMD.
IPR027682. MTSS1.
IPR003124. WH2_dom.
[Graphical view]
PANTHERPTHR15708:SF5. PTHR15708:SF5. 1 hit.
PfamPF08397. IMD. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTSM00246. WH2. 1 hit.
[Graphical view]
PROSITEPS51338. IMD. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO43312.
GeneWikiMTSS1.
GenomeRNAi9788.
NextBio36858.
PROO43312.
SOURCESearch...

Entry information

Entry nameMTSS1_HUMAN
AccessionPrimary (citable) accession number: O43312
Secondary accession number(s): Q8TCA2, Q96RX2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 16, 2003
Last modified: April 16, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM