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Protein

CBP80/20-dependent translation initiation factor

Gene

CTIF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD).1 Publication

GO - Molecular functioni

  1. RNA binding Source: InterPro

GO - Biological processi

  1. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
  2. regulation of translational initiation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Nonsense-mediated mRNA decay, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
CBP80/20-dependent translation initiation factor
Gene namesi
Name:CTIF
Synonyms:KIAA0427
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:23925. CTIF.

Subcellular locationi

  1. Cytoplasmperinuclear region 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134974369.

Polymorphism and mutation databases

BioMutaiCTIF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598CBP80/20-dependent translation initiation factorPRO_0000050754Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications
Modified residuei18 – 181PhosphoserineBy similarity
Modified residuei289 – 2891Phosphothreonine1 Publication
Modified residuei299 – 2991Phosphoserine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO43310.
PaxDbiO43310.
PRIDEiO43310.

PTM databases

PhosphoSiteiO43310.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiO43310.
CleanExiHS_KIAA0427.
ExpressionAtlasiO43310. baseline and differential.
GenevestigatoriO43310.

Organism-specific databases

HPAiHPA016865.
HPA024801.

Interactioni

Subunit structurei

Interacts with NCBP1/CBP80; the interaction is direct. Associates with the eukaryotic translation initiation factor 3 (eIF-3) complex.1 Publication

Protein-protein interaction databases

BioGridi115150. 5 interactions.
IntActiO43310. 1 interaction.
STRINGi9606.ENSP00000372459.

Structurei

3D structure databases

ProteinModelPortaliO43310.
SMRiO43310. Positions 377-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini376 – 577202MIF4GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 305305Interaction with NCBP1/CBP80Add
BLAST

Sequence similaritiesi

Belongs to the CTIF family.Curated
Contains 1 MIF4G domain.Curated

Phylogenomic databases

eggNOGiNOG305962.
GeneTreeiENSGT00630000089865.
HOGENOMiHOG000113148.
InParanoidiO43310.
OMAiLPAWQGY.
OrthoDBiEOG7JX33W.
PhylomeDBiO43310.
TreeFamiTF350740.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43310-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENSSAASAS SEAGSSRSQE IEELERFIDS YVLEYQVQGL LADKTEGDGE
60 70 80 90 100
SERTQSHISQ WTADCSEPLD SSCSFSRGRA PPQQNGSKDN SLDMLGTDIW
110 120 130 140 150
AANTFDSFSG ATWDLQPEKL DFTQFHRKVR HTPKQPLPHI DREGCGKGKL
160 170 180 190 200
EDGDGINLND IEKVLPAWQG YHPMPHEVEI AHTKKLFRRR RNDRRRQQRP
210 220 230 240 250
PGGNKPQQHG DHQPGSAKHN RDHQKSYQGG SAPHPSGRPT HHGYSQNRRW
260 270 280 290 300
HHGNMKHPPG DKGEAGAHRN AKETMTIENP KLEDTAGDTG HSSLEAPRSP
310 320 330 340 350
DTLAPVASER LPPQQSGGPE VETKRKDSIL PERIGERPKI TLLQSSKDRL
360 370 380 390 400
RRRLKEKDEV AVETTTPQQN KMDKLIEILN SMRNNSSDVD TKLTTFMEEA
410 420 430 440 450
QNSTNSEEML GEIVRTIYQK AVSDRSFAFT AAKLCDKMAL FMVEGTKFRS
460 470 480 490 500
LLLNMLQKDF TVREELQQQD VERWLGFITF LCEVFGTMRS STGEPFRVLV
510 520 530 540 550
CPIYTCLREL LQSQDVKEDA VLCCSMELQS TGRLLEEQLP EMMTELLASA
560 570 580 590
RDKMLCPSES MLTRSLLLEV IELHANSWNP LTPPITQYYN RTIQKLTA
Length:598
Mass (Da):67,587
Last modified:June 1, 1998 - v1
Checksum:i9F8776857A7DDC8A
GO
Isoform 2 (identifier: O43310-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-357: K → KVP

Note: No experimental confirmation available.

Show »
Length:600
Mass (Da):67,783
Checksum:iC1B11E2D07F44794
GO

Sequence cautioni

The sequence BAA24857.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAG53180.1 differs from that shown.Aberrant splicing.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti82 – 821P → L.
Corresponds to variant rs2277712 [ dbSNP | Ensembl ].
VAR_020041
Natural varianti389 – 3891V → L in a breast cancer sample; somatic mutation. 1 Publication
VAR_035749
Natural varianti438 – 4381M → I in a breast cancer sample; somatic mutation. 1 Publication
VAR_035750

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei357 – 3571K → KVP in isoform 2. 1 PublicationVSP_013774

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007887 mRNA. Translation: BAA24857.2. Different initiation.
AC022919 Genomic DNA. No translation available.
AC048380 Genomic DNA. No translation available.
AC093567 Genomic DNA. No translation available.
BC042146 mRNA. Translation: AAH42146.1.
AK095970 mRNA. Translation: BAG53180.1. Sequence problems.
CCDSiCCDS11935.1. [O43310-1]
CCDS45864.1. [O43310-2]
RefSeqiNP_001135869.1. NM_001142397.1. [O43310-2]
NP_055587.1. NM_014772.2. [O43310-1]
XP_006722650.1. XM_006722587.2. [O43310-2]
XP_006722651.1. XM_006722588.2. [O43310-2]
XP_006722652.1. XM_006722589.2. [O43310-2]
UniGeneiHs.145230.

Genome annotation databases

EnsembliENST00000256413; ENSP00000256413; ENSG00000134030. [O43310-1]
ENST00000382998; ENSP00000372459; ENSG00000134030. [O43310-2]
GeneIDi9811.
KEGGihsa:9811.
UCSCiuc002ldc.3. human. [O43310-1]
uc002ldd.3. human. [O43310-2]

Polymorphism and mutation databases

BioMutaiCTIF.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007887 mRNA. Translation: BAA24857.2. Different initiation.
AC022919 Genomic DNA. No translation available.
AC048380 Genomic DNA. No translation available.
AC093567 Genomic DNA. No translation available.
BC042146 mRNA. Translation: AAH42146.1.
AK095970 mRNA. Translation: BAG53180.1. Sequence problems.
CCDSiCCDS11935.1. [O43310-1]
CCDS45864.1. [O43310-2]
RefSeqiNP_001135869.1. NM_001142397.1. [O43310-2]
NP_055587.1. NM_014772.2. [O43310-1]
XP_006722650.1. XM_006722587.2. [O43310-2]
XP_006722651.1. XM_006722588.2. [O43310-2]
XP_006722652.1. XM_006722589.2. [O43310-2]
UniGeneiHs.145230.

3D structure databases

ProteinModelPortaliO43310.
SMRiO43310. Positions 377-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115150. 5 interactions.
IntActiO43310. 1 interaction.
STRINGi9606.ENSP00000372459.

PTM databases

PhosphoSiteiO43310.

Polymorphism and mutation databases

BioMutaiCTIF.

Proteomic databases

MaxQBiO43310.
PaxDbiO43310.
PRIDEiO43310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000256413; ENSP00000256413; ENSG00000134030. [O43310-1]
ENST00000382998; ENSP00000372459; ENSG00000134030. [O43310-2]
GeneIDi9811.
KEGGihsa:9811.
UCSCiuc002ldc.3. human. [O43310-1]
uc002ldd.3. human. [O43310-2]

Organism-specific databases

CTDi9811.
GeneCardsiGC18P046066.
HGNCiHGNC:23925. CTIF.
HPAiHPA016865.
HPA024801.
MIMi613178. gene.
neXtProtiNX_O43310.
PharmGKBiPA134974369.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG305962.
GeneTreeiENSGT00630000089865.
HOGENOMiHOG000113148.
InParanoidiO43310.
OMAiLPAWQGY.
OrthoDBiEOG7JX33W.
PhylomeDBiO43310.
TreeFamiTF350740.

Miscellaneous databases

ChiTaRSiCTIF. human.
GenomeRNAii9811.
NextBioi36936.
PROiO43310.
SOURCEiSearch...

Gene expression databases

BgeeiO43310.
CleanExiHS_KIAA0427.
ExpressionAtlasiO43310. baseline and differential.
GenevestigatoriO43310.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR016021. MIF4-like_typ_1/2/3.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
    Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-578 (ISOFORM 1).
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A new MIF4G domain-containing protein, CTIF, directs nuclear cap-binding protein CBP80/20-dependent translation."
    Kim K.M., Cho H., Choi K., Kim J., Kim B.-W., Ko Y.-G., Jang S.K., Kim Y.K.
    Genes Dev. 23:2033-2045(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NCBP1, INTERACTION WITH THE EIF-3 COMPLEX.
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-289 AND SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  13. Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-389 AND ILE-438.

Entry informationi

Entry nameiCTIF_HUMAN
AccessioniPrimary (citable) accession number: O43310
Secondary accession number(s): B3KTR8, Q8IVD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 1, 1998
Last modified: April 29, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.