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O43310

- CTIF_HUMAN

UniProt

O43310 - CTIF_HUMAN

Protein

CBP80/20-dependent translation initiation factor

Gene

CTIF

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD).1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
    2. regulation of translational initiation Source: UniProtKB

    Keywords - Biological processi

    Nonsense-mediated mRNA decay, Translation regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CBP80/20-dependent translation initiation factor
    Gene namesi
    Name:CTIF
    Synonyms:KIAA0427
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:23925. CTIF.

    Subcellular locationi

    Cytoplasmperinuclear region 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. perinuclear region of cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134974369.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 598598CBP80/20-dependent translation initiation factorPRO_0000050754Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications
    Modified residuei18 – 181PhosphoserineBy similarity
    Modified residuei299 – 2991Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO43310.
    PaxDbiO43310.
    PRIDEiO43310.

    PTM databases

    PhosphoSiteiO43310.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiO43310.
    BgeeiO43310.
    CleanExiHS_KIAA0427.
    GenevestigatoriO43310.

    Organism-specific databases

    HPAiHPA016865.
    HPA024801.

    Interactioni

    Subunit structurei

    Interacts with NCBP1/CBP80; the interaction is direct. Associates with the eukaryotic translation initiation factor 3 (eIF-3) complex.1 Publication

    Protein-protein interaction databases

    BioGridi115150. 3 interactions.
    IntActiO43310. 1 interaction.
    STRINGi9606.ENSP00000372459.

    Structurei

    3D structure databases

    ProteinModelPortaliO43310.
    SMRiO43310. Positions 377-589.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini376 – 577202MIF4GAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 305305Interaction with NCBP1/CBP80Add
    BLAST

    Sequence similaritiesi

    Belongs to the CTIF family.Curated
    Contains 1 MIF4G domain.Curated

    Phylogenomic databases

    eggNOGiNOG305962.
    HOGENOMiHOG000113148.
    OMAiPKQPLPH.
    OrthoDBiEOG7JX33W.
    PhylomeDBiO43310.
    TreeFamiTF350740.

    Family and domain databases

    Gene3Di1.25.40.180. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR016021. MIF4-like_typ_1/2/3.
    IPR003890. MIF4G-like_typ-3.
    [Graphical view]
    PfamiPF02854. MIF4G. 1 hit.
    [Graphical view]
    SMARTiSM00543. MIF4G. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O43310-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MENSSAASAS SEAGSSRSQE IEELERFIDS YVLEYQVQGL LADKTEGDGE    50
    SERTQSHISQ WTADCSEPLD SSCSFSRGRA PPQQNGSKDN SLDMLGTDIW 100
    AANTFDSFSG ATWDLQPEKL DFTQFHRKVR HTPKQPLPHI DREGCGKGKL 150
    EDGDGINLND IEKVLPAWQG YHPMPHEVEI AHTKKLFRRR RNDRRRQQRP 200
    PGGNKPQQHG DHQPGSAKHN RDHQKSYQGG SAPHPSGRPT HHGYSQNRRW 250
    HHGNMKHPPG DKGEAGAHRN AKETMTIENP KLEDTAGDTG HSSLEAPRSP 300
    DTLAPVASER LPPQQSGGPE VETKRKDSIL PERIGERPKI TLLQSSKDRL 350
    RRRLKEKDEV AVETTTPQQN KMDKLIEILN SMRNNSSDVD TKLTTFMEEA 400
    QNSTNSEEML GEIVRTIYQK AVSDRSFAFT AAKLCDKMAL FMVEGTKFRS 450
    LLLNMLQKDF TVREELQQQD VERWLGFITF LCEVFGTMRS STGEPFRVLV 500
    CPIYTCLREL LQSQDVKEDA VLCCSMELQS TGRLLEEQLP EMMTELLASA 550
    RDKMLCPSES MLTRSLLLEV IELHANSWNP LTPPITQYYN RTIQKLTA 598
    Length:598
    Mass (Da):67,587
    Last modified:June 1, 1998 - v1
    Checksum:i9F8776857A7DDC8A
    GO
    Isoform 2 (identifier: O43310-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         357-357: K → KVP

    Note: No experimental confirmation available.

    Show »
    Length:600
    Mass (Da):67,783
    Checksum:iC1B11E2D07F44794
    GO

    Sequence cautioni

    The sequence BAG53180.1 differs from that shown. Reason: Aberrant splicing.
    The sequence BAA24857.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti82 – 821P → L.
    Corresponds to variant rs2277712 [ dbSNP | Ensembl ].
    VAR_020041
    Natural varianti389 – 3891V → L in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035749
    Natural varianti438 – 4381M → I in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035750

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei357 – 3571K → KVP in isoform 2. 1 PublicationVSP_013774

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007887 mRNA. Translation: BAA24857.2. Different initiation.
    AC022919 Genomic DNA. No translation available.
    AC048380 Genomic DNA. No translation available.
    AC093567 Genomic DNA. No translation available.
    BC042146 mRNA. Translation: AAH42146.1.
    AK095970 mRNA. Translation: BAG53180.1. Sequence problems.
    CCDSiCCDS11935.1. [O43310-1]
    CCDS45864.1. [O43310-2]
    RefSeqiNP_001135869.1. NM_001142397.1. [O43310-2]
    NP_055587.1. NM_014772.2. [O43310-1]
    XP_006722650.1. XM_006722587.1. [O43310-2]
    XP_006722651.1. XM_006722588.1. [O43310-2]
    XP_006722652.1. XM_006722589.1. [O43310-2]
    UniGeneiHs.145230.

    Genome annotation databases

    EnsembliENST00000256413; ENSP00000256413; ENSG00000134030. [O43310-1]
    ENST00000382998; ENSP00000372459; ENSG00000134030. [O43310-2]
    GeneIDi9811.
    KEGGihsa:9811.
    UCSCiuc002ldc.3. human. [O43310-1]
    uc002ldd.3. human. [O43310-2]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007887 mRNA. Translation: BAA24857.2 . Different initiation.
    AC022919 Genomic DNA. No translation available.
    AC048380 Genomic DNA. No translation available.
    AC093567 Genomic DNA. No translation available.
    BC042146 mRNA. Translation: AAH42146.1 .
    AK095970 mRNA. Translation: BAG53180.1 . Sequence problems.
    CCDSi CCDS11935.1. [O43310-1 ]
    CCDS45864.1. [O43310-2 ]
    RefSeqi NP_001135869.1. NM_001142397.1. [O43310-2 ]
    NP_055587.1. NM_014772.2. [O43310-1 ]
    XP_006722650.1. XM_006722587.1. [O43310-2 ]
    XP_006722651.1. XM_006722588.1. [O43310-2 ]
    XP_006722652.1. XM_006722589.1. [O43310-2 ]
    UniGenei Hs.145230.

    3D structure databases

    ProteinModelPortali O43310.
    SMRi O43310. Positions 377-589.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115150. 3 interactions.
    IntActi O43310. 1 interaction.
    STRINGi 9606.ENSP00000372459.

    PTM databases

    PhosphoSitei O43310.

    Proteomic databases

    MaxQBi O43310.
    PaxDbi O43310.
    PRIDEi O43310.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000256413 ; ENSP00000256413 ; ENSG00000134030 . [O43310-1 ]
    ENST00000382998 ; ENSP00000372459 ; ENSG00000134030 . [O43310-2 ]
    GeneIDi 9811.
    KEGGi hsa:9811.
    UCSCi uc002ldc.3. human. [O43310-1 ]
    uc002ldd.3. human. [O43310-2 ]

    Organism-specific databases

    CTDi 9811.
    GeneCardsi GC18P046066.
    HGNCi HGNC:23925. CTIF.
    HPAi HPA016865.
    HPA024801.
    MIMi 613178. gene.
    neXtProti NX_O43310.
    PharmGKBi PA134974369.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG305962.
    HOGENOMi HOG000113148.
    OMAi PKQPLPH.
    OrthoDBi EOG7JX33W.
    PhylomeDBi O43310.
    TreeFami TF350740.

    Miscellaneous databases

    ChiTaRSi CTIF. human.
    GenomeRNAii 9811.
    NextBioi 36936.
    PROi O43310.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O43310.
    Bgeei O43310.
    CleanExi HS_KIAA0427.
    Genevestigatori O43310.

    Family and domain databases

    Gene3Di 1.25.40.180. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR016021. MIF4-like_typ_1/2/3.
    IPR003890. MIF4G-like_typ-3.
    [Graphical view ]
    Pfami PF02854. MIF4G. 1 hit.
    [Graphical view ]
    SMARTi SM00543. MIF4G. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
      Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-578 (ISOFORM 1).
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Platelet.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A new MIF4G domain-containing protein, CTIF, directs nuclear cap-binding protein CBP80/20-dependent translation."
      Kim K.M., Cho H., Choi K., Kim J., Kim B.-W., Ko Y.-G., Jang S.K., Kim Y.K.
      Genes Dev. 23:2033-2045(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NCBP1, INTERACTION WITH THE EIF-3 COMPLEX.
    9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-389 AND ILE-438.

    Entry informationi

    Entry nameiCTIF_HUMAN
    AccessioniPrimary (citable) accession number: O43310
    Secondary accession number(s): B3KTR8, Q8IVD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3