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Protein

CBP80/20-dependent translation initiation factor

Gene

CTIF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD).1 Publication

GO - Molecular functioni

GO - Biological processi

  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
  • regulation of translational initiation Source: UniProtKB

Keywordsi

Biological processNonsense-mediated mRNA decay, Translation regulation

Protein family/group databases

TCDBi1.I.1.1.3. the eukaryotic nuclear pore complex (e-npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
CBP80/20-dependent translation initiation factor
Gene namesi
Name:CTIF
Synonyms:KIAA0427
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000134030.13.
HGNCiHGNC:23925. CTIF.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi9811.
OpenTargetsiENSG00000134030.
PharmGKBiPA134974369.

Polymorphism and mutation databases

BioMutaiCTIF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000507541 – 598CBP80/20-dependent translation initiation factorAdd BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei18PhosphoserineCombined sources1
Modified residuei289PhosphothreonineCombined sources1
Modified residuei299PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO43310.
MaxQBiO43310.
PaxDbiO43310.
PeptideAtlasiO43310.
PRIDEiO43310.

PTM databases

iPTMnetiO43310.
PhosphoSitePlusiO43310.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000134030.
CleanExiHS_KIAA0427.
ExpressionAtlasiO43310. baseline and differential.
GenevisibleiO43310. HS.

Organism-specific databases

HPAiHPA016865.
HPA024801.

Interactioni

Subunit structurei

Interacts with NCBP1/CBP80; the interaction is direct. Associates with the eukaryotic translation initiation factor 3 (eIF-3) complex.1 Publication

Protein-protein interaction databases

BioGridi115150. 5 interactors.
CORUMiO43310.
IntActiO43310. 10 interactors.
STRINGi9606.ENSP00000372459.

Structurei

3D structure databases

ProteinModelPortaliO43310.
SMRiO43310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini376 – 577MIF4GAdd BLAST202

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 305Interaction with NCBP1/CBP801 PublicationAdd BLAST305

Sequence similaritiesi

Belongs to the CTIF family.Curated

Phylogenomic databases

eggNOGiKOG3942. Eukaryota.
ENOG410XQKW. LUCA.
GeneTreeiENSGT00630000089865.
HOGENOMiHOG000113148.
InParanoidiO43310.
OMAiQYYNKTI.
OrthoDBiEOG091G05G0.
PhylomeDBiO43310.
TreeFamiTF350740.

Family and domain databases

InterProiView protein in InterPro
IPR016024. ARM-type_fold.
IPR003890. MIF4G-like_typ-3.
PfamiView protein in Pfam
PF02854. MIF4G. 1 hit.
SMARTiView protein in SMART
SM00543. MIF4G. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43310-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENSSAASAS SEAGSSRSQE IEELERFIDS YVLEYQVQGL LADKTEGDGE
60 70 80 90 100
SERTQSHISQ WTADCSEPLD SSCSFSRGRA PPQQNGSKDN SLDMLGTDIW
110 120 130 140 150
AANTFDSFSG ATWDLQPEKL DFTQFHRKVR HTPKQPLPHI DREGCGKGKL
160 170 180 190 200
EDGDGINLND IEKVLPAWQG YHPMPHEVEI AHTKKLFRRR RNDRRRQQRP
210 220 230 240 250
PGGNKPQQHG DHQPGSAKHN RDHQKSYQGG SAPHPSGRPT HHGYSQNRRW
260 270 280 290 300
HHGNMKHPPG DKGEAGAHRN AKETMTIENP KLEDTAGDTG HSSLEAPRSP
310 320 330 340 350
DTLAPVASER LPPQQSGGPE VETKRKDSIL PERIGERPKI TLLQSSKDRL
360 370 380 390 400
RRRLKEKDEV AVETTTPQQN KMDKLIEILN SMRNNSSDVD TKLTTFMEEA
410 420 430 440 450
QNSTNSEEML GEIVRTIYQK AVSDRSFAFT AAKLCDKMAL FMVEGTKFRS
460 470 480 490 500
LLLNMLQKDF TVREELQQQD VERWLGFITF LCEVFGTMRS STGEPFRVLV
510 520 530 540 550
CPIYTCLREL LQSQDVKEDA VLCCSMELQS TGRLLEEQLP EMMTELLASA
560 570 580 590
RDKMLCPSES MLTRSLLLEV IELHANSWNP LTPPITQYYN RTIQKLTA
Length:598
Mass (Da):67,587
Last modified:June 1, 1998 - v1
Checksum:i9F8776857A7DDC8A
GO
Isoform 2 (identifier: O43310-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-357: K → KVP

Note: No experimental confirmation available.
Show »
Length:600
Mass (Da):67,783
Checksum:iC1B11E2D07F44794
GO

Sequence cautioni

The sequence BAA24857 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAG53180 differs from that shown. Aberrant splicing.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02004182P → L. Corresponds to variant dbSNP:rs2277712Ensembl.1
Natural variantiVAR_035749389V → L in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_035750438M → I in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013774357K → KVP in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007887 mRNA. Translation: BAA24857.2. Different initiation.
AC022919 Genomic DNA. No translation available.
AC048380 Genomic DNA. No translation available.
AC093567 Genomic DNA. No translation available.
BC042146 mRNA. Translation: AAH42146.1.
AK095970 mRNA. Translation: BAG53180.1. Sequence problems.
CCDSiCCDS11935.1. [O43310-1]
CCDS45864.1. [O43310-2]
RefSeqiNP_001135869.1. NM_001142397.1. [O43310-2]
NP_055587.1. NM_014772.2. [O43310-1]
XP_006722650.1. XM_006722587.3. [O43310-2]
XP_006722651.1. XM_006722588.3. [O43310-2]
XP_006722652.1. XM_006722589.3. [O43310-2]
XP_011524581.1. XM_011526279.2. [O43310-1]
XP_016881591.1. XM_017026102.1. [O43310-2]
UniGeneiHs.145230.

Genome annotation databases

EnsembliENST00000256413; ENSP00000256413; ENSG00000134030. [O43310-1]
ENST00000382998; ENSP00000372459; ENSG00000134030. [O43310-2]
ENST00000634350; ENSP00000489354; ENSG00000282825. [O43310-2]
ENST00000635228; ENSP00000489589; ENSG00000282825. [O43310-1]
GeneIDi9811.
KEGGihsa:9811.
UCSCiuc002ldc.4. human. [O43310-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCTIF_HUMAN
AccessioniPrimary (citable) accession number: O43310
Secondary accession number(s): B3KTR8, Q8IVD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 1, 1998
Last modified: September 27, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families