O43310 (CTIF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CBP80/20-dependent translation initiation factor

Gene names

Name:	CTIF
Synonyms:	KIAA0427

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD). Ref.7
Subunit structure	Interacts with NCBP1/CBP80; the interaction is direct. Associates with the eukaryotic translation initiation factor 3 (eIF-3) complex. Ref.7
Subcellular location	Cytoplasm › perinuclear region Ref.7.
Tissue specificity	Widely expressed. Ref.7
Sequence similarities	Belongs to the CTIF family. Contains 1 MIF4G domain.
Sequence caution	The sequence BAA24857.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAG53180.1 differs from that shown. Reason: Aberrant splicing.

Ontologies

Keywords
Biological process	Nonsense-mediated mRNA decay Translation regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Inferred from mutant phenotype Ref.7. Source: UniProtKB regulation of translational initiation Inferred from mutant phenotype Ref.7. Source: UniProtKB
Cellular_component	perinuclear region of cytoplasm Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43310-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43310-2)

The sequence of this isoform differs from the canonical sequence as follows:
     357-357: K → KVP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	CBP80/20-dependent translation initiation factor		PRO_0000050754
Regions
	Domain	376 – 577	202	MIF4G
	Region	1 – 305	305	Interaction with NCBP1/CBP80
Amino acid modifications
	Modified residue	18	1	Phosphoserine By similarity
	Modified residue	299	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	357	1	K → KVP in isoform 2.		VSP_013774
	Natural variant	82	1	P → L. Corresponds to variant rs2277712 [ dbSNP | Ensembl ].		VAR_020041
	Natural variant	389	1	V → L in a breast cancer sample; somatic mutation. Ref.10		VAR_035749
	Natural variant	438	1	M → I in a breast cancer sample; somatic mutation. Ref.10		VAR_035750

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9F8776857A7DDC8A	FASTA	598	67,587
	10 20 30 40 50 60 MENSSAASAS SEAGSSRSQE IEELERFIDS YVLEYQVQGL LADKTEGDGE SERTQSHISQ 70 80 90 100 110 120 WTADCSEPLD SSCSFSRGRA PPQQNGSKDN SLDMLGTDIW AANTFDSFSG ATWDLQPEKL 130 140 150 160 170 180 DFTQFHRKVR HTPKQPLPHI DREGCGKGKL EDGDGINLND IEKVLPAWQG YHPMPHEVEI 190 200 210 220 230 240 AHTKKLFRRR RNDRRRQQRP PGGNKPQQHG DHQPGSAKHN RDHQKSYQGG SAPHPSGRPT 250 260 270 280 290 300 HHGYSQNRRW HHGNMKHPPG DKGEAGAHRN AKETMTIENP KLEDTAGDTG HSSLEAPRSP 310 320 330 340 350 360 DTLAPVASER LPPQQSGGPE VETKRKDSIL PERIGERPKI TLLQSSKDRL RRRLKEKDEV 370 380 390 400 410 420 AVETTTPQQN KMDKLIEILN SMRNNSSDVD TKLTTFMEEA QNSTNSEEML GEIVRTIYQK 430 440 450 460 470 480 AVSDRSFAFT AAKLCDKMAL FMVEGTKFRS LLLNMLQKDF TVREELQQQD VERWLGFITF 490 500 510 520 530 540 LCEVFGTMRS STGEPFRVLV CPIYTCLREL LQSQDVKEDA VLCCSMELQS TGRLLEEQLP 550 560 570 580 590 EMMTELLASA RDKMLCPSES MLTRSLLLEV IELHANSWNP LTPPITQYYN RTIQKLTA
	Isoform 2 [UniParc]. Checksum: C1B11E2D07F44794	FASTA	600	67,783
	10 20 30 40 50 60 MENSSAASAS SEAGSSRSQE IEELERFIDS YVLEYQVQGL LADKTEGDGE SERTQSHISQ 70 80 90 100 110 120 WTADCSEPLD SSCSFSRGRA PPQQNGSKDN SLDMLGTDIW AANTFDSFSG ATWDLQPEKL 130 140 150 160 170 180 DFTQFHRKVR HTPKQPLPHI DREGCGKGKL EDGDGINLND IEKVLPAWQG YHPMPHEVEI 190 200 210 220 230 240 AHTKKLFRRR RNDRRRQQRP PGGNKPQQHG DHQPGSAKHN RDHQKSYQGG SAPHPSGRPT 250 260 270 280 290 300 HHGYSQNRRW HHGNMKHPPG DKGEAGAHRN AKETMTIENP KLEDTAGDTG HSSLEAPRSP 310 320 330 340 350 360 DTLAPVASER LPPQQSGGPE VETKRKDSIL PERIGERPKI TLLQSSKDRL RRRLKEKVPD 370 380 390 400 410 420 EVAVETTTPQ QNKMDKLIEI LNSMRNNSSD VDTKLTTFME EAQNSTNSEE MLGEIVRTIY 430 440 450 460 470 480 QKAVSDRSFA FTAAKLCDKM ALFMVEGTKF RSLLLNMLQK DFTVREELQQ QDVERWLGFI 490 500 510 520 530 540 TFLCEVFGTM RSSTGEPFRV LVCPIYTCLR ELLQSQDVKE DAVLCCSMEL QSTGRLLEEQ 550 560 570 580 590 600 LPEMMTELLA SARDKMLCPS ESMLTRSLLL EVIELHANSW NPLTPPITQY YNRTIQKLTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro." Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-578 (ISOFORM 1).
[5]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, MASS SPECTROMETRY. Tissue: Platelet.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"A new MIF4G domain-containing protein, CTIF, directs nuclear cap-binding protein CBP80/20-dependent translation." Kim K.M., Cho H., Choi K., Kim J., Kim B.-W., Ko Y.-G., Jang S.K., Kim Y.K. Genes Dev. 23:2033-2045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH NCBP1, INTERACTION WITH THE EIF-3 COMPLEX.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-389 AND ILE-438.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007887 mRNA. Translation: BAA24857.2. Different initiation. AC022919 Genomic DNA. No translation available. AC048380 Genomic DNA. No translation available. AC093567 Genomic DNA. No translation available. BC042146 mRNA. Translation: AAH42146.1. AK095970 mRNA. Translation: BAG53180.1. Sequence problems.
IPI	IPI00011951. IPI00604690. IPI00902575.
RefSeq	NP_001135869.1. NM_001142397.1. NP_055587.1. NM_014772.2.
UniGene	Hs.145230.
3D structure databases
ProteinModelPortal	O43310.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43310. 1 interaction.
STRING	9606.ENSP00000372459.
PTM databases
PhosphoSite	O43310.
Proteomic databases
PaxDb	O43310.
PRIDE	O43310.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000256413; ENSP00000256413; ENSG00000134030. ENST00000382998; ENSP00000372459; ENSG00000134030.
GeneID	9811.
KEGG	hsa:9811.
UCSC	uc002ldc.3. human. uc002ldd.3. human.
Organism-specific databases
CTD	9811.
GeneCards	GC18P046066.
HGNC	HGNC:23925. CTIF.
HPA	HPA016865. HPA024801.
MIM	613178. gene.
neXtProt	NX_O43310.
PharmGKB	PA134974369.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG305962.
HOGENOM	HOG000113148.
OMA	TQFHRKL.
OrthoDB	EOG4CVG6J.
PhylomeDB	O43310.
Gene expression databases
Bgee	O43310.
CleanEx	HS_KIAA0427.
Genevestigator	O43310.
GermOnline	ENSG00000134030. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.180. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR016021. MIF4-like_typ_1/2/3. IPR003890. MIF4G-like_typ-3. [Graphical view]
Pfam	PF02854. MIF4G. 1 hit. [Graphical view]
SMART	SM00543. MIF4G. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	CTIF. human.
GenomeRNAi	9811.
NextBio	36936.
SOURCE	Search...

Entry information

Entry name

CTIF_HUMAN

Accession

Primary (citable) accession number: O43310
Secondary accession number(s): B3KTR8, Q8IVD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.