O43290 (SNUT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U4/U6.U5 tri-snRNP-associated protein 1

Alternative name(s):
SNU66 homolog
Short name=hSnu66
Squamous cell carcinoma antigen recognized by T-cells 1
Short name=SART-1
Short name=hSART-1
U4/U6.U5 tri-snRNP-associated 110 kDa protein
Allergen=Hom s 1

Gene names

Name:

SART1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	800 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. Ref.2
Subunit structure	Identified in the spliceosome C complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Ref.2 Ref.9 Ref.11
Subcellular location	Nucleus. Note: Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells. Ref.1 Ref.2
Tissue specificity	Ubiquitously expressed. Ref.1
Post-translational modification	Sumoylated with SUMO2.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations. Ref.8
Sequence similarities	Belongs to the SNU66/SART1 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Allergen
Domain	Coiled coil
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle arrest Non-traceable author statement PubMed 16158934. Source: UniProtKB intrinsic apoptotic signaling pathway Non-traceable author statement PubMed 16158934. Source: UniProtKB positive regulation of cytotoxic T cell differentiation Inferred from direct assay PubMed 10209962. Source: UniProtKB spliceosomal snRNP assembly Traceable author statement PubMed 16687569. Source: BHF-UCL
Cellular_component	Cajal body Inferred from direct assay PubMed 16687569. Source: BHF-UCL Golgi apparatus Inferred from direct assay. Source: HPA catalytic step 2 spliceosome Inferred from direct assay Ref.9. Source: UniProtKB cytosol Traceable author statement PubMed 10929426. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SUMO1	P63165	2	EBI-607761,EBI-80140
SUMO3	P55854	2	EBI-607761,EBI-474067

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms may be produced.

Isoform 1 (identifier: O43290-1)

Also known as: 125 kDa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 800	800	U4/U6.U5 tri-snRNP-associated protein 1		PRO_0000223308
Regions
	Coiled coil	157 – 231	75	Potential
	Coiled coil	490 – 533	44	Potential
Amino acid modifications
	Modified residue	392	1	Phosphothreonine Ref.14
	Modified residue	430	1	Phosphothreonine Ref.12 Ref.15
	Modified residue	448	1	Phosphoserine Ref.10 Ref.13 Ref.14 Ref.15 Ref.16 Ref.18
	Modified residue	521	1	Phosphoserine Ref.16
	Modified residue	591	1	Phosphoserine Ref.10 Ref.16
	Modified residue	596	1	Phosphoserine Ref.16
	Modified residue	598	1	Phosphoserine Ref.16
	Modified residue	695	1	Phosphothreonine Ref.12
	Modified residue	764	1	Phosphothreonine Ref.15
Natural variations
	Natural variant	245	1	R → C. Corresponds to variant rs688862 [ dbSNP | Ensembl ].		VAR_051367
	Natural variant	463	1	S → A. Corresponds to variant rs35036096 [ dbSNP | Ensembl ].		VAR_034504
	Natural variant	485	1	G → A. Ref.4 Corresponds to variant rs660118 [ dbSNP | Ensembl ].		VAR_025319
Experimental info
	Sequence conflict	209	1	Q → R in BAD96736. Ref.4
	Sequence conflict	712	1	Y → H in BAD96736. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (125 kDa) [UniParc]. Last modified June 1, 1998. Version 1. Checksum: B13525E80640DF9E	FASTA	800	90,255
	10 20 30 40 50 60 MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG 70 80 90 100 110 120 GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD GYEAAASSKT SSGDASSLSI 130 140 150 160 170 180 EETNKLRAKL GLKPLEVNAI KKEAGTKEEP VTADVINPMA LRQREELREK LAAAKEKRLL 190 200 210 220 230 240 NQKLGKIKTL GEDDPWLDDT AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE 250 260 270 280 290 300 FGQRRQDLYS ARDLQGLTVE HAIDSFREGE TMILTLKDKG VLQEEEDVLV NVNLVDKERA 310 320 330 340 350 360 EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGERPHSFR LEQGGTADGL 370 380 390 400 410 420 RERELEEIRA KLRLQAQSLS TVGPRLASEY LTPEEMVTFK KTKRRVKKIR KKEKEVVVRA 430 440 450 460 470 480 DDLLPLGDQT QDGDFGSRLR GRGRRRVSEV EEEKEPVPQP LPSDDTRVEN MDISDEEEGG 490 500 510 520 530 540 APPPGSPQVL EEDEAELELQ KQLEKGRRLR QLQQLQQLRD SGEKVVEIVK KLESRQRGWE 550 560 570 580 590 600 EDEDPERKGA IVFNATSEFC RTLGEIPTYG LAGNREEQEE LMDFERDEER SANGGSESDG 610 620 630 640 650 660 EENIGWSTVN LDEEKQQQDF SASSTTILDE EPIVNRGLAA ALLLCQNKGL LETTVQKVAR 670 680 690 700 710 720 VKAPNKSLPS AVYCIEDKMA IDDKYSRREE YRGFTQDFKE KDGYKPDVKI EYVDETGRKL 730 740 750 760 770 780 TPKEAFRQLS HRFHGKGSGK MKTERRMKKL DEEALLKKMS SSDTPLGTVA LLQEKQKAQK 790 800 TPYIVLSGSG KSMNANTITK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene encoding antigenic peptides of human squamous cell carcinoma recognized cytotoxic T lymphocytes." Shichijo S., Nakao M., Imai Y., Takasu H., Kawamoto M., Niiya F., Yang D., Toh Y., Yamana H., Itoh K. J. Exp. Med. 187:277-288(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"The 65 and 110 kDa SR-related proteins of the U4/U6*U5 tri-snRNP are essential for the assembly of mature spliceosomes." Makarova O.V., Makarov E.M., Luehrmann R. EMBO J. 20:2553-2563(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, SUBUNIT.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-485. Tissue: Small intestine.
[5]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[7]	"Intron loss in the SART1 genes of Fugu rubripes and Tetraodon nigroviridis." Bolland D.J., Hewitt J.E. Gene 271:43-49(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 639-732.
[8]	"Molecular characterization of an autoallergen, Hom s 1, identified by serum IgE from atopic dermatitis patients." Valenta R., Natter S., Seiberler S., Wichlas S., Maurer D., Hess M., Pavelka M., Grote M., Ferreira F., Szepfalusi Z., Valent P., Stingl G. J. Invest. Dermatol. 111:1178-1183(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
[9]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448 AND SER-591, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP." Liu S., Rauhut R., Vornlocher H.-P., Luehrmann R. RNA 12:1418-1430(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[12]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430 AND THR-695, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[13]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-392 AND SER-448, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430; SER-448 AND THR-764, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[16]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448; SER-521; SER-591; SER-596 AND SER-598, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[17]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB006198 mRNA. Translation: BAA24056.1. AF353625 mRNA. Translation: AAK49523.1. BT006637 mRNA. Translation: AAP35283.1. AK223016 mRNA. Translation: BAD96736.1. AP006287 Genomic DNA. No translation available. BC001058 mRNA. Translation: AAH01058.1. AF109680 Genomic DNA. Translation: AAD20223.1.
IPI	IPI00021417.
PIR	T00034.
RefSeq	NP_005137.1. NM_005146.4.
UniGene	Hs.502883.
3D structure databases
ProteinModelPortal	O43290.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-33360N.
IntAct	O43290. 11 interactions.
MINT	MINT-1630512.
STRING	9606.ENSP00000310448.
Protein family/group databases
Allergome	3322. Hom s 1.0101. 411. Hom s 1.
PTM databases
PhosphoSite	O43290.
Proteomic databases
PaxDb	O43290.
PeptideAtlas	O43290.
PRIDE	O43290.
Protocols and materials databases
DNASU	9092.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312397; ENSP00000310448; ENSG00000175467.
GeneID	9092.
KEGG	hsa:9092.
UCSC	uc001ogl.3. human.
Organism-specific databases
CTD	9092.
GeneCards	GC11P065730.
H-InvDB	HIX0095875.
HGNC	HGNC:10538. SART1.
HPA	HPA031188. HPA031189. HPA031190.
MIM	605941. gene.
neXtProt	NX_O43290.
PharmGKB	PA34947.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG268163.
HOGENOM	HOG000046291.
HOVERGEN	HBG052942.
InParanoid	O43290.
KO	K11984.
OMA	VAADVIN.
OrthoDB	EOG4W9J3H.
Gene expression databases
ArrayExpress	O43290.
Bgee	O43290.
CleanEx	HS_SART1.
Genevestigator	O43290.
GermOnline	ENSG00000175467. Homo sapiens.
Family and domain databases
InterPro	IPR005011. SART_1. [Graphical view]
PANTHER	PTHR14152. PTHR14152. 1 hit.
Pfam	PF03343. SART-1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SART1. human.
GenomeRNAi	9092.
NextBio	34067.
SOURCE	Search...

Entry information

Entry name

SNUT1_HUMAN

Accession

Primary (citable) accession number: O43290
Secondary accession number(s): A6NDN1, Q53GB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.