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O43290

- SNUT1_HUMAN

UniProt

O43290 - SNUT1_HUMAN

Protein

U4/U6.U5 tri-snRNP-associated protein 1

Gene

SART1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.1 Publication

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle arrest Source: UniProtKB
    2. intrinsic apoptotic signaling pathway Source: UniProtKB
    3. mRNA splicing, via spliceosome Source: UniProtKB
    4. positive regulation of cytotoxic T cell differentiation Source: UniProtKB
    5. spliceosomal snRNP assembly Source: BHF-UCL

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U4/U6.U5 tri-snRNP-associated protein 1
    Alternative name(s):
    SNU66 homolog
    Short name:
    hSnu66
    Squamous cell carcinoma antigen recognized by T-cells 1
    Short name:
    SART-1
    Short name:
    hSART-1
    U4/U6.U5 tri-snRNP-associated 110 kDa protein
    Allergen: Hom s 1
    Gene namesi
    Name:SART1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:10538. SART1.

    Subcellular locationi

    Nucleus 2 Publications
    Note: Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells.

    GO - Cellular componenti

    1. Cajal body Source: BHF-UCL
    2. catalytic step 2 spliceosome Source: UniProtKB
    3. cytoplasm Source: ProtInc
    4. cytosol Source: UniProtKB
    5. Golgi apparatus Source: HPA
    6. nucleoplasm Source: BHF-UCL
    7. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations.1 Publication

    Keywords - Diseasei

    Allergen

    Organism-specific databases

    PharmGKBiPA34947.

    Protein family/group databases

    Allergomei3322. Hom s 1.0101.
    411. Hom s 1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 800800U4/U6.U5 tri-snRNP-associated protein 1PRO_0000223308Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei392 – 3921Phosphothreonine1 Publication
    Modified residuei430 – 4301Phosphothreonine2 Publications
    Modified residuei448 – 4481Phosphoserine6 Publications
    Modified residuei521 – 5211Phosphoserine1 Publication
    Modified residuei591 – 5911Phosphoserine2 Publications
    Modified residuei596 – 5961Phosphoserine1 Publication
    Modified residuei598 – 5981Phosphoserine1 Publication
    Modified residuei695 – 6951Phosphothreonine1 Publication
    Modified residuei764 – 7641Phosphothreonine1 Publication

    Post-translational modificationi

    Sumoylated with SUMO2.

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiO43290.
    PaxDbiO43290.
    PeptideAtlasiO43290.
    PRIDEiO43290.

    PTM databases

    PhosphoSiteiO43290.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    ArrayExpressiO43290.
    BgeeiO43290.
    CleanExiHS_SART1.
    GenevestigatoriO43290.

    Organism-specific databases

    HPAiHPA031188.
    HPA031189.
    HPA031190.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SNW1Q135733EBI-607761,EBI-632715
    SUMO1P631652EBI-607761,EBI-80140
    SUMO3P558542EBI-607761,EBI-474067

    Protein-protein interaction databases

    BioGridi114546. 89 interactions.
    DIPiDIP-33360N.
    IntActiO43290. 21 interactions.
    MINTiMINT-1630512.
    STRINGi9606.ENSP00000310448.

    Structurei

    3D structure databases

    ProteinModelPortaliO43290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili157 – 23175Sequence AnalysisAdd
    BLAST
    Coiled coili490 – 53344Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SNU66/SART1 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG268163.
    HOGENOMiHOG000046291.
    HOVERGENiHBG052942.
    InParanoidiO43290.
    KOiK11984.
    OMAiFDDTSEF.
    OrthoDBiEOG7S7SF7.
    PhylomeDBiO43290.
    TreeFamiTF318828.

    Family and domain databases

    InterProiIPR005011. SART_1.
    [Graphical view]
    PANTHERiPTHR14152. PTHR14152. 1 hit.
    PfamiPF03343. SART-1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms may be produced.

    Isoform 1 (identifier: O43290-1) [UniParc]FASTAAdd to Basket

    Also known as: 125 kDa

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG    50
    ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD 100
    GYEAAASSKT SSGDASSLSI EETNKLRAKL GLKPLEVNAI KKEAGTKEEP 150
    VTADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT 200
    AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FGQRRQDLYS 250
    ARDLQGLTVE HAIDSFREGE TMILTLKDKG VLQEEEDVLV NVNLVDKERA 300
    EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGERPHSFR 350
    LEQGGTADGL RERELEEIRA KLRLQAQSLS TVGPRLASEY LTPEEMVTFK 400
    KTKRRVKKIR KKEKEVVVRA DDLLPLGDQT QDGDFGSRLR GRGRRRVSEV 450
    EEEKEPVPQP LPSDDTRVEN MDISDEEEGG APPPGSPQVL EEDEAELELQ 500
    KQLEKGRRLR QLQQLQQLRD SGEKVVEIVK KLESRQRGWE EDEDPERKGA 550
    IVFNATSEFC RTLGEIPTYG LAGNREEQEE LMDFERDEER SANGGSESDG 600
    EENIGWSTVN LDEEKQQQDF SASSTTILDE EPIVNRGLAA ALLLCQNKGL 650
    LETTVQKVAR VKAPNKSLPS AVYCIEDKMA IDDKYSRREE YRGFTQDFKE 700
    KDGYKPDVKI EYVDETGRKL TPKEAFRQLS HRFHGKGSGK MKTERRMKKL 750
    DEEALLKKMS SSDTPLGTVA LLQEKQKAQK TPYIVLSGSG KSMNANTITK 800
    Length:800
    Mass (Da):90,255
    Last modified:June 1, 1998 - v1
    Checksum:iB13525E80640DF9E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti209 – 2091Q → R in BAD96736. 1 PublicationCurated
    Sequence conflicti712 – 7121Y → H in BAD96736. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti245 – 2451R → C.
    Corresponds to variant rs688862 [ dbSNP | Ensembl ].
    VAR_051367
    Natural varianti463 – 4631S → A.
    Corresponds to variant rs35036096 [ dbSNP | Ensembl ].
    VAR_034504
    Natural varianti485 – 4851G → A.1 Publication
    Corresponds to variant rs660118 [ dbSNP | Ensembl ].
    VAR_025319

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006198 mRNA. Translation: BAA24056.1.
    AF353625 mRNA. Translation: AAK49523.1.
    BT006637 mRNA. Translation: AAP35283.1.
    AK223016 mRNA. Translation: BAD96736.1.
    AP006287 Genomic DNA. No translation available.
    BC001058 mRNA. Translation: AAH01058.1.
    AF109680 Genomic DNA. Translation: AAD20223.1.
    CCDSiCCDS31611.1. [O43290-1]
    PIRiT00034.
    RefSeqiNP_005137.1. NM_005146.4. [O43290-1]
    UniGeneiHs.502883.

    Genome annotation databases

    EnsembliENST00000312397; ENSP00000310448; ENSG00000175467. [O43290-1]
    GeneIDi9092.
    KEGGihsa:9092.
    UCSCiuc001ogl.3. human. [O43290-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006198 mRNA. Translation: BAA24056.1 .
    AF353625 mRNA. Translation: AAK49523.1 .
    BT006637 mRNA. Translation: AAP35283.1 .
    AK223016 mRNA. Translation: BAD96736.1 .
    AP006287 Genomic DNA. No translation available.
    BC001058 mRNA. Translation: AAH01058.1 .
    AF109680 Genomic DNA. Translation: AAD20223.1 .
    CCDSi CCDS31611.1. [O43290-1 ]
    PIRi T00034.
    RefSeqi NP_005137.1. NM_005146.4. [O43290-1 ]
    UniGenei Hs.502883.

    3D structure databases

    ProteinModelPortali O43290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114546. 89 interactions.
    DIPi DIP-33360N.
    IntActi O43290. 21 interactions.
    MINTi MINT-1630512.
    STRINGi 9606.ENSP00000310448.

    Protein family/group databases

    Allergomei 3322. Hom s 1.0101.
    411. Hom s 1.

    PTM databases

    PhosphoSitei O43290.

    Proteomic databases

    MaxQBi O43290.
    PaxDbi O43290.
    PeptideAtlasi O43290.
    PRIDEi O43290.

    Protocols and materials databases

    DNASUi 9092.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000312397 ; ENSP00000310448 ; ENSG00000175467 . [O43290-1 ]
    GeneIDi 9092.
    KEGGi hsa:9092.
    UCSCi uc001ogl.3. human. [O43290-1 ]

    Organism-specific databases

    CTDi 9092.
    GeneCardsi GC11P065730.
    H-InvDB HIX0095875.
    HGNCi HGNC:10538. SART1.
    HPAi HPA031188.
    HPA031189.
    HPA031190.
    MIMi 605941. gene.
    neXtProti NX_O43290.
    PharmGKBi PA34947.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG268163.
    HOGENOMi HOG000046291.
    HOVERGENi HBG052942.
    InParanoidi O43290.
    KOi K11984.
    OMAi FDDTSEF.
    OrthoDBi EOG7S7SF7.
    PhylomeDBi O43290.
    TreeFami TF318828.

    Miscellaneous databases

    ChiTaRSi SART1. human.
    GeneWikii SART1.
    GenomeRNAii 9092.
    NextBioi 34067.
    PROi O43290.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O43290.
    Bgeei O43290.
    CleanExi HS_SART1.
    Genevestigatori O43290.

    Family and domain databases

    InterProi IPR005011. SART_1.
    [Graphical view ]
    PANTHERi PTHR14152. PTHR14152. 1 hit.
    Pfami PF03343. SART-1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A gene encoding antigenic peptides of human squamous cell carcinoma recognized cytotoxic T lymphocytes."
      Shichijo S., Nakao M., Imai Y., Takasu H., Kawamoto M., Niiya F., Yang D., Toh Y., Yamana H., Itoh K.
      J. Exp. Med. 187:277-288(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    2. "The 65 and 110 kDa SR-related proteins of the U4/U6*U5 tri-snRNP are essential for the assembly of mature spliceosomes."
      Makarova O.V., Makarov E.M., Luehrmann R.
      EMBO J. 20:2553-2563(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, SUBUNIT.
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-485.
      Tissue: Small intestine.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    7. "Intron loss in the SART1 genes of Fugu rubripes and Tetraodon nigroviridis."
      Bolland D.J., Hewitt J.E.
      Gene 271:43-49(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 639-732.
    8. "Molecular characterization of an autoallergen, Hom s 1, identified by serum IgE from atopic dermatitis patients."
      Valenta R., Natter S., Seiberler S., Wichlas S., Maurer D., Hess M., Pavelka M., Grote M., Ferreira F., Szepfalusi Z., Valent P., Stingl G.
      J. Invest. Dermatol. 111:1178-1183(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
    9. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
    10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448 AND SER-591, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP."
      Liu S., Rauhut R., Vornlocher H.-P., Luehrmann R.
      RNA 12:1418-1430(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430 AND THR-695, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    13. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    14. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-392 AND SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430; SER-448 AND THR-764, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    17. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448; SER-521; SER-591; SER-596 AND SER-598, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    18. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    19. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSNUT1_HUMAN
    AccessioniPrimary (citable) accession number: O43290
    Secondary accession number(s): A6NDN1, Q53GB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3