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O43290

- SNUT1_HUMAN

UniProt

O43290 - SNUT1_HUMAN

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Protein

U4/U6.U5 tri-snRNP-associated protein 1

Gene

SART1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.1 Publication

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  1. cell cycle arrest Source: UniProtKB
  2. intrinsic apoptotic signaling pathway Source: UniProtKB
  3. mRNA splicing, via spliceosome Source: UniProtKB
  4. positive regulation of cytotoxic T cell differentiation Source: UniProtKB
  5. spliceosomal snRNP assembly Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6.U5 tri-snRNP-associated protein 1
Alternative name(s):
SNU66 homolog
Short name:
hSnu66
Squamous cell carcinoma antigen recognized by T-cells 1
Short name:
SART-1
Short name:
hSART-1
U4/U6.U5 tri-snRNP-associated 110 kDa protein
Allergen: Hom s 1
Gene namesi
Name:SART1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:10538. SART1.

Subcellular locationi

Nucleus 2 Publications
Note: Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells.

GO - Cellular componenti

  1. Cajal body Source: BHF-UCL
  2. catalytic step 2 spliceosome Source: UniProtKB
  3. cytoplasm Source: ProtInc
  4. cytosol Source: UniProtKB
  5. Golgi apparatus Source: HPA
  6. nucleoplasm Source: BHF-UCL
  7. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations.1 Publication

Keywords - Diseasei

Allergen

Organism-specific databases

PharmGKBiPA34947.

Protein family/group databases

Allergomei3322. Hom s 1.0101.
411. Hom s 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 800800U4/U6.U5 tri-snRNP-associated protein 1PRO_0000223308Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei392 – 3921Phosphothreonine1 Publication
Modified residuei430 – 4301Phosphothreonine2 Publications
Modified residuei448 – 4481Phosphoserine6 Publications
Modified residuei521 – 5211Phosphoserine1 Publication
Modified residuei591 – 5911Phosphoserine2 Publications
Modified residuei596 – 5961Phosphoserine1 Publication
Modified residuei598 – 5981Phosphoserine1 Publication
Modified residuei695 – 6951Phosphothreonine1 Publication
Modified residuei764 – 7641Phosphothreonine1 Publication

Post-translational modificationi

Sumoylated with SUMO2.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO43290.
PaxDbiO43290.
PeptideAtlasiO43290.
PRIDEiO43290.

PTM databases

PhosphoSiteiO43290.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiO43290.
CleanExiHS_SART1.
ExpressionAtlasiO43290. baseline and differential.
GenevestigatoriO43290.

Organism-specific databases

HPAiHPA031188.
HPA031189.
HPA031190.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNW1Q135733EBI-607761,EBI-632715
SUMO1P631652EBI-607761,EBI-80140
SUMO3P558542EBI-607761,EBI-474067

Protein-protein interaction databases

BioGridi114546. 94 interactions.
DIPiDIP-33360N.
IntActiO43290. 21 interactions.
MINTiMINT-1630512.
STRINGi9606.ENSP00000310448.

Structurei

Secondary structure

1
800
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi120 – 12910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4PYUX-ray2.00C/D/H/L/P/T117-135[»]
ProteinModelPortaliO43290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili157 – 23175Sequence AnalysisAdd
BLAST
Coiled coili490 – 53344Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SNU66/SART1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG268163.
GeneTreeiENSGT00390000007071.
HOGENOMiHOG000046291.
HOVERGENiHBG052942.
InParanoidiO43290.
KOiK11984.
OMAiFDDTSEF.
OrthoDBiEOG7S7SF7.
PhylomeDBiO43290.
TreeFamiTF318828.

Family and domain databases

InterProiIPR005011. SART_1.
[Graphical view]
PANTHERiPTHR14152. PTHR14152. 1 hit.
PfamiPF03343. SART-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms may be produced.

Isoform 1 (identifier: O43290-1) [UniParc]FASTAAdd to Basket

Also known as: 125 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG
60 70 80 90 100
ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD
110 120 130 140 150
GYEAAASSKT SSGDASSLSI EETNKLRAKL GLKPLEVNAI KKEAGTKEEP
160 170 180 190 200
VTADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT
210 220 230 240 250
AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FGQRRQDLYS
260 270 280 290 300
ARDLQGLTVE HAIDSFREGE TMILTLKDKG VLQEEEDVLV NVNLVDKERA
310 320 330 340 350
EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGERPHSFR
360 370 380 390 400
LEQGGTADGL RERELEEIRA KLRLQAQSLS TVGPRLASEY LTPEEMVTFK
410 420 430 440 450
KTKRRVKKIR KKEKEVVVRA DDLLPLGDQT QDGDFGSRLR GRGRRRVSEV
460 470 480 490 500
EEEKEPVPQP LPSDDTRVEN MDISDEEEGG APPPGSPQVL EEDEAELELQ
510 520 530 540 550
KQLEKGRRLR QLQQLQQLRD SGEKVVEIVK KLESRQRGWE EDEDPERKGA
560 570 580 590 600
IVFNATSEFC RTLGEIPTYG LAGNREEQEE LMDFERDEER SANGGSESDG
610 620 630 640 650
EENIGWSTVN LDEEKQQQDF SASSTTILDE EPIVNRGLAA ALLLCQNKGL
660 670 680 690 700
LETTVQKVAR VKAPNKSLPS AVYCIEDKMA IDDKYSRREE YRGFTQDFKE
710 720 730 740 750
KDGYKPDVKI EYVDETGRKL TPKEAFRQLS HRFHGKGSGK MKTERRMKKL
760 770 780 790 800
DEEALLKKMS SSDTPLGTVA LLQEKQKAQK TPYIVLSGSG KSMNANTITK
Length:800
Mass (Da):90,255
Last modified:June 1, 1998 - v1
Checksum:iB13525E80640DF9E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti209 – 2091Q → R in BAD96736. 1 PublicationCurated
Sequence conflicti712 – 7121Y → H in BAD96736. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti245 – 2451R → C.
Corresponds to variant rs688862 [ dbSNP | Ensembl ].
VAR_051367
Natural varianti463 – 4631S → A.
Corresponds to variant rs35036096 [ dbSNP | Ensembl ].
VAR_034504
Natural varianti485 – 4851G → A.1 Publication
Corresponds to variant rs660118 [ dbSNP | Ensembl ].
VAR_025319

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006198 mRNA. Translation: BAA24056.1.
AF353625 mRNA. Translation: AAK49523.1.
BT006637 mRNA. Translation: AAP35283.1.
AK223016 mRNA. Translation: BAD96736.1.
AP006287 Genomic DNA. No translation available.
BC001058 mRNA. Translation: AAH01058.1.
AF109680 Genomic DNA. Translation: AAD20223.1.
CCDSiCCDS31611.1. [O43290-1]
PIRiT00034.
RefSeqiNP_005137.1. NM_005146.4. [O43290-1]
UniGeneiHs.502883.

Genome annotation databases

EnsembliENST00000312397; ENSP00000310448; ENSG00000175467. [O43290-1]
GeneIDi9092.
KEGGihsa:9092.
UCSCiuc001ogl.3. human. [O43290-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006198 mRNA. Translation: BAA24056.1 .
AF353625 mRNA. Translation: AAK49523.1 .
BT006637 mRNA. Translation: AAP35283.1 .
AK223016 mRNA. Translation: BAD96736.1 .
AP006287 Genomic DNA. No translation available.
BC001058 mRNA. Translation: AAH01058.1 .
AF109680 Genomic DNA. Translation: AAD20223.1 .
CCDSi CCDS31611.1. [O43290-1 ]
PIRi T00034.
RefSeqi NP_005137.1. NM_005146.4. [O43290-1 ]
UniGenei Hs.502883.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4PYU X-ray 2.00 C/D/H/L/P/T 117-135 [» ]
ProteinModelPortali O43290.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114546. 94 interactions.
DIPi DIP-33360N.
IntActi O43290. 21 interactions.
MINTi MINT-1630512.
STRINGi 9606.ENSP00000310448.

Protein family/group databases

Allergomei 3322. Hom s 1.0101.
411. Hom s 1.

PTM databases

PhosphoSitei O43290.

Proteomic databases

MaxQBi O43290.
PaxDbi O43290.
PeptideAtlasi O43290.
PRIDEi O43290.

Protocols and materials databases

DNASUi 9092.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000312397 ; ENSP00000310448 ; ENSG00000175467 . [O43290-1 ]
GeneIDi 9092.
KEGGi hsa:9092.
UCSCi uc001ogl.3. human. [O43290-1 ]

Organism-specific databases

CTDi 9092.
GeneCardsi GC11P065730.
H-InvDB HIX0095875.
HGNCi HGNC:10538. SART1.
HPAi HPA031188.
HPA031189.
HPA031190.
MIMi 605941. gene.
neXtProti NX_O43290.
PharmGKBi PA34947.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG268163.
GeneTreei ENSGT00390000007071.
HOGENOMi HOG000046291.
HOVERGENi HBG052942.
InParanoidi O43290.
KOi K11984.
OMAi FDDTSEF.
OrthoDBi EOG7S7SF7.
PhylomeDBi O43290.
TreeFami TF318828.

Miscellaneous databases

ChiTaRSi SART1. human.
GeneWikii SART1.
GenomeRNAii 9092.
NextBioi 34067.
PROi O43290.
SOURCEi Search...

Gene expression databases

Bgeei O43290.
CleanExi HS_SART1.
ExpressionAtlasi O43290. baseline and differential.
Genevestigatori O43290.

Family and domain databases

InterProi IPR005011. SART_1.
[Graphical view ]
PANTHERi PTHR14152. PTHR14152. 1 hit.
Pfami PF03343. SART-1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A gene encoding antigenic peptides of human squamous cell carcinoma recognized cytotoxic T lymphocytes."
    Shichijo S., Nakao M., Imai Y., Takasu H., Kawamoto M., Niiya F., Yang D., Toh Y., Yamana H., Itoh K.
    J. Exp. Med. 187:277-288(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  2. "The 65 and 110 kDa SR-related proteins of the U4/U6*U5 tri-snRNP are essential for the assembly of mature spliceosomes."
    Makarova O.V., Makarov E.M., Luehrmann R.
    EMBO J. 20:2553-2563(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, SUBUNIT.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-485.
    Tissue: Small intestine.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  7. "Intron loss in the SART1 genes of Fugu rubripes and Tetraodon nigroviridis."
    Bolland D.J., Hewitt J.E.
    Gene 271:43-49(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 639-732.
  8. "Molecular characterization of an autoallergen, Hom s 1, identified by serum IgE from atopic dermatitis patients."
    Valenta R., Natter S., Seiberler S., Wichlas S., Maurer D., Hess M., Pavelka M., Grote M., Ferreira F., Szepfalusi Z., Valent P., Stingl G.
    J. Invest. Dermatol. 111:1178-1183(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
  9. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
    Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
    RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
  10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448 AND SER-591, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP."
    Liu S., Rauhut R., Vornlocher H.-P., Luehrmann R.
    RNA 12:1418-1430(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430 AND THR-695, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  13. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  14. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-392 AND SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430; SER-448 AND THR-764, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  17. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448; SER-521; SER-591; SER-596 AND SER-598, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  18. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  19. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSNUT1_HUMAN
AccessioniPrimary (citable) accession number: O43290
Secondary accession number(s): A6NDN1, Q53GB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3