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Protein

U4/U6.U5 tri-snRNP-associated protein 1

Gene

SART1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.1 Publication

GO - Molecular functioni

GO - Biological processi

  • maturation of 5S rRNA Source: GO_Central
  • mRNA cis splicing, via spliceosome Source: GO_Central
  • mRNA splicing, via spliceosome Source: Reactome
  • positive regulation of cytotoxic T cell differentiation Source: UniProtKB
  • spliceosomal snRNP assembly Source: BHF-UCL

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6.U5 tri-snRNP-associated protein 1
Alternative name(s):
SNU66 homolog
Short name:
hSnu66
Squamous cell carcinoma antigen recognized by T-cells 1
Short name:
SART-1
Short name:
hSART-1
U4/U6.U5 tri-snRNP-associated 110 kDa protein
Allergen: Hom s 1
Gene namesi
Name:SART1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000175467.14
HGNCiHGNC:10538 SART1
MIMi605941 gene
neXtProtiNX_O43290

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations.1 Publication

Keywords - Diseasei

Allergen

Organism-specific databases

DisGeNETi9092
OpenTargetsiENSG00000175467
PharmGKBiPA34947

Protein family/group databases

Allergomei3322 Hom s 1.0101
411 Hom s 1

Polymorphism and mutation databases

BioMutaiSART1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002233081 – 800U4/U6.U5 tri-snRNP-associated protein 1Add BLAST800

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki125Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki188Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei189PhosphothreonineBy similarity1
Cross-linki277Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei321PhosphoserineCombined sources1
Cross-linki329Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki336Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei348PhosphoserineCombined sources1
Modified residuei392PhosphothreonineCombined sources1
Cross-linki400Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki414Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei430PhosphothreonineCombined sources1
Modified residuei448PhosphoserineCombined sources1
Modified residuei474PhosphoserineCombined sources1
Modified residuei486PhosphoserineCombined sources1
Modified residuei521PhosphoserineCombined sources1
Cross-linki548Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei591PhosphoserineCombined sources1
Modified residuei596PhosphoserineCombined sources1
Modified residuei598PhosphoserineCombined sources1
Modified residuei621PhosphoserineCombined sources1
Cross-linki648Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki657Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki684Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei695PhosphothreonineCombined sources1
Cross-linki699Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki709Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki723Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki749Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki758Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei761PhosphoserineCombined sources1
Modified residuei764PhosphothreonineCombined sources1
Cross-linki775Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki780Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei789PhosphoserineCombined sources1
Cross-linki791Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Sumoylated with SUMO2.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO43290
MaxQBiO43290
PaxDbiO43290
PeptideAtlasiO43290
PRIDEiO43290

PTM databases

iPTMnetiO43290
PhosphoSitePlusiO43290

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000175467
CleanExiHS_SART1
ExpressionAtlasiO43290 baseline and differential
GenevisibleiO43290 HS

Organism-specific databases

HPAiHPA031188
HPA031189
HPA031190

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114546124 interactors.
CORUMiO43290
DIPiDIP-33360N
IntActiO43290 35 interactors.
MINTiO43290
STRINGi9606.ENSP00000310448

Structurei

Secondary structure

1800
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi120 – 129Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PYUX-ray2.00C/D/H/L/P/T117-135[»]
5O9Zelectron microscopy4.50P1-800[»]
ProteinModelPortaliO43290
SMRiO43290
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili157 – 231Sequence analysisAdd BLAST75
Coiled coili490 – 533Sequence analysisAdd BLAST44

Sequence similaritiesi

Belongs to the SNU66/SART1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2217 Eukaryota
ENOG410XSTT LUCA
GeneTreeiENSGT00390000007071
HOGENOMiHOG000046291
HOVERGENiHBG052942
InParanoidiO43290
KOiK11984
OMAiARWENKQ
OrthoDBiEOG091G04IE
PhylomeDBiO43290
TreeFamiTF318828

Family and domain databases

InterProiView protein in InterPro
IPR005011 SNU66/SART1
PANTHERiPTHR14152 PTHR14152, 1 hit
PfamiView protein in Pfam
PF03343 SART-1, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms may be produced.
Isoform 1 (identifier: O43290-1) [UniParc]FASTAAdd to basket
Also known as: 125 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG
60 70 80 90 100
ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD
110 120 130 140 150
GYEAAASSKT SSGDASSLSI EETNKLRAKL GLKPLEVNAI KKEAGTKEEP
160 170 180 190 200
VTADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT
210 220 230 240 250
AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FGQRRQDLYS
260 270 280 290 300
ARDLQGLTVE HAIDSFREGE TMILTLKDKG VLQEEEDVLV NVNLVDKERA
310 320 330 340 350
EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGERPHSFR
360 370 380 390 400
LEQGGTADGL RERELEEIRA KLRLQAQSLS TVGPRLASEY LTPEEMVTFK
410 420 430 440 450
KTKRRVKKIR KKEKEVVVRA DDLLPLGDQT QDGDFGSRLR GRGRRRVSEV
460 470 480 490 500
EEEKEPVPQP LPSDDTRVEN MDISDEEEGG APPPGSPQVL EEDEAELELQ
510 520 530 540 550
KQLEKGRRLR QLQQLQQLRD SGEKVVEIVK KLESRQRGWE EDEDPERKGA
560 570 580 590 600
IVFNATSEFC RTLGEIPTYG LAGNREEQEE LMDFERDEER SANGGSESDG
610 620 630 640 650
EENIGWSTVN LDEEKQQQDF SASSTTILDE EPIVNRGLAA ALLLCQNKGL
660 670 680 690 700
LETTVQKVAR VKAPNKSLPS AVYCIEDKMA IDDKYSRREE YRGFTQDFKE
710 720 730 740 750
KDGYKPDVKI EYVDETGRKL TPKEAFRQLS HRFHGKGSGK MKTERRMKKL
760 770 780 790 800
DEEALLKKMS SSDTPLGTVA LLQEKQKAQK TPYIVLSGSG KSMNANTITK
Length:800
Mass (Da):90,255
Last modified:June 1, 1998 - v1
Checksum:iB13525E80640DF9E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209Q → R in BAD96736 (Ref. 4) Curated1
Sequence conflicti712Y → H in BAD96736 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051367245R → C. Corresponds to variant dbSNP:rs688862Ensembl.1
Natural variantiVAR_034504463S → A. Corresponds to variant dbSNP:rs35036096Ensembl.1
Natural variantiVAR_025319485G → A1 PublicationCorresponds to variant dbSNP:rs660118Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006198 mRNA Translation: BAA24056.1
AF353625 mRNA Translation: AAK49523.1
BT006637 mRNA Translation: AAP35283.1
AK223016 mRNA Translation: BAD96736.1
AP006287 Genomic DNA No translation available.
BC001058 mRNA Translation: AAH01058.1
AF109680 Genomic DNA Translation: AAD20223.1
CCDSiCCDS31611.1 [O43290-1]
PIRiT00034
RefSeqiNP_005137.1, NM_005146.4 [O43290-1]
UniGeneiHs.502883

Genome annotation databases

EnsembliENST00000312397; ENSP00000310448; ENSG00000175467 [O43290-1]
GeneIDi9092
KEGGihsa:9092
UCSCiuc001ogl.4 human [O43290-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSNUT1_HUMAN
AccessioniPrimary (citable) accession number: O43290
Secondary accession number(s): A6NDN1, Q53GB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome