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Protein

U4/U6.U5 tri-snRNP-associated protein 1

Gene

SART1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.1 Publication

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • cell cycle arrest Source: UniProtKB
  • intrinsic apoptotic signaling pathway Source: UniProtKB
  • maturation of 5S rRNA Source: GO_Central
  • mRNA cis splicing, via spliceosome Source: GO_Central
  • mRNA splicing, via spliceosome Source: UniProtKB
  • positive regulation of cytotoxic T cell differentiation Source: UniProtKB
  • spliceosomal snRNP assembly Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175467-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6.U5 tri-snRNP-associated protein 1
Alternative name(s):
SNU66 homolog
Short name:
hSnu66
Squamous cell carcinoma antigen recognized by T-cells 1
Short name:
SART-1
Short name:
hSART-1
U4/U6.U5 tri-snRNP-associated 110 kDa protein
Allergen: Hom s 1
Gene namesi
Name:SART1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:10538. SART1.

Subcellular locationi

GO - Cellular componenti

  • Cajal body Source: BHF-UCL
  • catalytic step 2 spliceosome Source: UniProtKB
  • cytoplasm Source: ProtInc
  • cytosol Source: UniProtKB
  • Golgi apparatus Source: HPA
  • nucleoplasm Source: BHF-UCL
  • U4/U6 x U5 tri-snRNP complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations.1 Publication

Keywords - Diseasei

Allergen

Organism-specific databases

DisGeNETi9092.
OpenTargetsiENSG00000175467.
PharmGKBiPA34947.

Protein family/group databases

Allergomei3322. Hom s 1.0101.
411. Hom s 1.

Polymorphism and mutation databases

BioMutaiSART1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002233081 – 800U4/U6.U5 tri-snRNP-associated protein 1Add BLAST800

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki188Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei189PhosphothreonineBy similarity1
Modified residuei321PhosphoserineCombined sources1
Modified residuei348PhosphoserineCombined sources1
Modified residuei392PhosphothreonineCombined sources1
Modified residuei430PhosphothreonineCombined sources1
Modified residuei448PhosphoserineCombined sources1
Modified residuei474PhosphoserineCombined sources1
Modified residuei486PhosphoserineCombined sources1
Modified residuei521PhosphoserineCombined sources1
Modified residuei591PhosphoserineCombined sources1
Modified residuei596PhosphoserineCombined sources1
Modified residuei598PhosphoserineCombined sources1
Modified residuei621PhosphoserineCombined sources1
Modified residuei695PhosphothreonineCombined sources1
Modified residuei761PhosphoserineCombined sources1
Modified residuei764PhosphothreonineCombined sources1
Modified residuei789PhosphoserineCombined sources1

Post-translational modificationi

Sumoylated with SUMO2.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO43290.
MaxQBiO43290.
PaxDbiO43290.
PeptideAtlasiO43290.
PRIDEiO43290.

PTM databases

iPTMnetiO43290.
PhosphoSitePlusiO43290.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000175467.
CleanExiHS_SART1.
ExpressionAtlasiO43290. baseline and differential.
GenevisibleiO43290. HS.

Organism-specific databases

HPAiHPA031188.
HPA031189.
HPA031190.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNW1Q135733EBI-607761,EBI-632715
SUMO1P631652EBI-607761,EBI-80140
SUMO3P558542EBI-607761,EBI-474067
UBL5Q9BZL14EBI-607761,EBI-607755

Protein-protein interaction databases

BioGridi114546. 117 interactors.
DIPiDIP-33360N.
IntActiO43290. 32 interactors.
MINTiMINT-1630512.
STRINGi9606.ENSP00000310448.

Structurei

Secondary structure

1800
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi120 – 129Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PYUX-ray2.00C/D/H/L/P/T117-135[»]
ProteinModelPortaliO43290.
SMRiO43290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili157 – 231Sequence analysisAdd BLAST75
Coiled coili490 – 533Sequence analysisAdd BLAST44

Sequence similaritiesi

Belongs to the SNU66/SART1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2217. Eukaryota.
ENOG410XSTT. LUCA.
GeneTreeiENSGT00390000007071.
HOGENOMiHOG000046291.
HOVERGENiHBG052942.
InParanoidiO43290.
KOiK11984.
OMAiFVRAITY.
OrthoDBiEOG091G04IE.
PhylomeDBiO43290.
TreeFamiTF318828.

Family and domain databases

InterProiIPR005011. SNU66/SART1.
[Graphical view]
PANTHERiPTHR14152:SF5. PTHR14152:SF5. 1 hit.
PfamiPF03343. SART-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms may be produced.
Isoform 1 (identifier: O43290-1) [UniParc]FASTAAdd to basket
Also known as: 125 kDa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG
60 70 80 90 100
ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD
110 120 130 140 150
GYEAAASSKT SSGDASSLSI EETNKLRAKL GLKPLEVNAI KKEAGTKEEP
160 170 180 190 200
VTADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT
210 220 230 240 250
AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FGQRRQDLYS
260 270 280 290 300
ARDLQGLTVE HAIDSFREGE TMILTLKDKG VLQEEEDVLV NVNLVDKERA
310 320 330 340 350
EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGERPHSFR
360 370 380 390 400
LEQGGTADGL RERELEEIRA KLRLQAQSLS TVGPRLASEY LTPEEMVTFK
410 420 430 440 450
KTKRRVKKIR KKEKEVVVRA DDLLPLGDQT QDGDFGSRLR GRGRRRVSEV
460 470 480 490 500
EEEKEPVPQP LPSDDTRVEN MDISDEEEGG APPPGSPQVL EEDEAELELQ
510 520 530 540 550
KQLEKGRRLR QLQQLQQLRD SGEKVVEIVK KLESRQRGWE EDEDPERKGA
560 570 580 590 600
IVFNATSEFC RTLGEIPTYG LAGNREEQEE LMDFERDEER SANGGSESDG
610 620 630 640 650
EENIGWSTVN LDEEKQQQDF SASSTTILDE EPIVNRGLAA ALLLCQNKGL
660 670 680 690 700
LETTVQKVAR VKAPNKSLPS AVYCIEDKMA IDDKYSRREE YRGFTQDFKE
710 720 730 740 750
KDGYKPDVKI EYVDETGRKL TPKEAFRQLS HRFHGKGSGK MKTERRMKKL
760 770 780 790 800
DEEALLKKMS SSDTPLGTVA LLQEKQKAQK TPYIVLSGSG KSMNANTITK
Length:800
Mass (Da):90,255
Last modified:June 1, 1998 - v1
Checksum:iB13525E80640DF9E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209Q → R in BAD96736 (Ref. 4) Curated1
Sequence conflicti712Y → H in BAD96736 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051367245R → C.Corresponds to variant rs688862dbSNPEnsembl.1
Natural variantiVAR_034504463S → A.Corresponds to variant rs35036096dbSNPEnsembl.1
Natural variantiVAR_025319485G → A.1 PublicationCorresponds to variant rs660118dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006198 mRNA. Translation: BAA24056.1.
AF353625 mRNA. Translation: AAK49523.1.
BT006637 mRNA. Translation: AAP35283.1.
AK223016 mRNA. Translation: BAD96736.1.
AP006287 Genomic DNA. No translation available.
BC001058 mRNA. Translation: AAH01058.1.
AF109680 Genomic DNA. Translation: AAD20223.1.
CCDSiCCDS31611.1. [O43290-1]
PIRiT00034.
RefSeqiNP_005137.1. NM_005146.4. [O43290-1]
UniGeneiHs.502883.

Genome annotation databases

EnsembliENST00000312397; ENSP00000310448; ENSG00000175467. [O43290-1]
GeneIDi9092.
KEGGihsa:9092.
UCSCiuc001ogl.4. human. [O43290-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006198 mRNA. Translation: BAA24056.1.
AF353625 mRNA. Translation: AAK49523.1.
BT006637 mRNA. Translation: AAP35283.1.
AK223016 mRNA. Translation: BAD96736.1.
AP006287 Genomic DNA. No translation available.
BC001058 mRNA. Translation: AAH01058.1.
AF109680 Genomic DNA. Translation: AAD20223.1.
CCDSiCCDS31611.1. [O43290-1]
PIRiT00034.
RefSeqiNP_005137.1. NM_005146.4. [O43290-1]
UniGeneiHs.502883.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PYUX-ray2.00C/D/H/L/P/T117-135[»]
ProteinModelPortaliO43290.
SMRiO43290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114546. 117 interactors.
DIPiDIP-33360N.
IntActiO43290. 32 interactors.
MINTiMINT-1630512.
STRINGi9606.ENSP00000310448.

Protein family/group databases

Allergomei3322. Hom s 1.0101.
411. Hom s 1.

PTM databases

iPTMnetiO43290.
PhosphoSitePlusiO43290.

Polymorphism and mutation databases

BioMutaiSART1.

Proteomic databases

EPDiO43290.
MaxQBiO43290.
PaxDbiO43290.
PeptideAtlasiO43290.
PRIDEiO43290.

Protocols and materials databases

DNASUi9092.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312397; ENSP00000310448; ENSG00000175467. [O43290-1]
GeneIDi9092.
KEGGihsa:9092.
UCSCiuc001ogl.4. human. [O43290-1]

Organism-specific databases

CTDi9092.
DisGeNETi9092.
GeneCardsiSART1.
H-InvDBHIX0095875.
HGNCiHGNC:10538. SART1.
HPAiHPA031188.
HPA031189.
HPA031190.
MIMi605941. gene.
neXtProtiNX_O43290.
OpenTargetsiENSG00000175467.
PharmGKBiPA34947.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2217. Eukaryota.
ENOG410XSTT. LUCA.
GeneTreeiENSGT00390000007071.
HOGENOMiHOG000046291.
HOVERGENiHBG052942.
InParanoidiO43290.
KOiK11984.
OMAiFVRAITY.
OrthoDBiEOG091G04IE.
PhylomeDBiO43290.
TreeFamiTF318828.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175467-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiSART1. human.
GeneWikiiSART1.
GenomeRNAii9092.
PROiO43290.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175467.
CleanExiHS_SART1.
ExpressionAtlasiO43290. baseline and differential.
GenevisibleiO43290. HS.

Family and domain databases

InterProiIPR005011. SNU66/SART1.
[Graphical view]
PANTHERiPTHR14152:SF5. PTHR14152:SF5. 1 hit.
PfamiPF03343. SART-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNUT1_HUMAN
AccessioniPrimary (citable) accession number: O43290
Secondary accession number(s): A6NDN1, Q53GB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.