Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O43290 (SNUT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U4/U6.U5 tri-snRNP-associated protein 1
Alternative name(s):
SNU66 homolog
Short name=hSnu66
Squamous cell carcinoma antigen recognized by T-cells 1
Short name=SART-1
Short name=hSART-1
U4/U6.U5 tri-snRNP-associated 110 kDa protein
Allergen=Hom s 1
Gene names
Name:SART1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length800 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. Ref.2

Subunit structure

Identified in the spliceosome C complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Ref.2 Ref.9 Ref.11

Subcellular location

Nucleus. Note: Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells. Ref.1 Ref.2

Tissue specificity

Ubiquitously expressed. Ref.1

Post-translational modification

Sumoylated with SUMO2.

Allergenic properties

Causes an allergic reaction in human. Binds to IgE from atopic dermatitis (AD) patients. Identified as an IgE autoantigen in atopic dermatitis (AD) patients with severe skin manifestations. Ref.8

Sequence similarities

Belongs to the SNU66/SART1 family.

Binary interactions

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms may be produced.
Isoform 1 (identifier: O43290-1)

Also known as: 125 kDa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 800800U4/U6.U5 tri-snRNP-associated protein 1
PRO_0000223308

Regions

Coiled coil157 – 23175 Potential
Coiled coil490 – 53344 Potential

Amino acid modifications

Modified residue3921Phosphothreonine Ref.14
Modified residue4301Phosphothreonine Ref.12 Ref.16
Modified residue4481Phosphoserine Ref.10 Ref.13 Ref.14 Ref.16 Ref.17 Ref.19
Modified residue5211Phosphoserine Ref.17
Modified residue5911Phosphoserine Ref.10 Ref.17
Modified residue5961Phosphoserine Ref.17
Modified residue5981Phosphoserine Ref.17
Modified residue6951Phosphothreonine Ref.12
Modified residue7641Phosphothreonine Ref.16

Natural variations

Natural variant2451R → C.
Corresponds to variant rs688862 [ dbSNP | Ensembl ].
VAR_051367
Natural variant4631S → A.
Corresponds to variant rs35036096 [ dbSNP | Ensembl ].
VAR_034504
Natural variant4851G → A. Ref.4
Corresponds to variant rs660118 [ dbSNP | Ensembl ].
VAR_025319

Experimental info

Sequence conflict2091Q → R in BAD96736. Ref.4
Sequence conflict7121Y → H in BAD96736. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (125 kDa) [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: B13525E80640DF9E

FASTA80090,255
        10         20         30         40         50         60 
MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG 

        70         80         90        100        110        120 
GERGSGRRGA EAEARSSTHG RERSQAEPSE RRVKREKRDD GYEAAASSKT SSGDASSLSI 

       130        140        150        160        170        180 
EETNKLRAKL GLKPLEVNAI KKEAGTKEEP VTADVINPMA LRQREELREK LAAAKEKRLL 

       190        200        210        220        230        240 
NQKLGKIKTL GEDDPWLDDT AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE 

       250        260        270        280        290        300 
FGQRRQDLYS ARDLQGLTVE HAIDSFREGE TMILTLKDKG VLQEEEDVLV NVNLVDKERA 

       310        320        330        340        350        360 
EKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILSKYDEE LEGERPHSFR LEQGGTADGL 

       370        380        390        400        410        420 
RERELEEIRA KLRLQAQSLS TVGPRLASEY LTPEEMVTFK KTKRRVKKIR KKEKEVVVRA 

       430        440        450        460        470        480 
DDLLPLGDQT QDGDFGSRLR GRGRRRVSEV EEEKEPVPQP LPSDDTRVEN MDISDEEEGG 

       490        500        510        520        530        540 
APPPGSPQVL EEDEAELELQ KQLEKGRRLR QLQQLQQLRD SGEKVVEIVK KLESRQRGWE 

       550        560        570        580        590        600 
EDEDPERKGA IVFNATSEFC RTLGEIPTYG LAGNREEQEE LMDFERDEER SANGGSESDG 

       610        620        630        640        650        660 
EENIGWSTVN LDEEKQQQDF SASSTTILDE EPIVNRGLAA ALLLCQNKGL LETTVQKVAR 

       670        680        690        700        710        720 
VKAPNKSLPS AVYCIEDKMA IDDKYSRREE YRGFTQDFKE KDGYKPDVKI EYVDETGRKL 

       730        740        750        760        770        780 
TPKEAFRQLS HRFHGKGSGK MKTERRMKKL DEEALLKKMS SSDTPLGTVA LLQEKQKAQK 

       790        800 
TPYIVLSGSG KSMNANTITK 

« Hide

References

« Hide 'large scale' references
[1]"A gene encoding antigenic peptides of human squamous cell carcinoma recognized cytotoxic T lymphocytes."
Shichijo S., Nakao M., Imai Y., Takasu H., Kawamoto M., Niiya F., Yang D., Toh Y., Yamana H., Itoh K.
J. Exp. Med. 187:277-288(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]"The 65 and 110 kDa SR-related proteins of the U4/U6*U5 tri-snRNP are essential for the assembly of mature spliceosomes."
Makarova O.V., Makarov E.M., Luehrmann R.
EMBO J. 20:2553-2563(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, SUBUNIT.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-485.
Tissue: Small intestine.
[5]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[7]"Intron loss in the SART1 genes of Fugu rubripes and Tetraodon nigroviridis."
Bolland D.J., Hewitt J.E.
Gene 271:43-49(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 639-732.
[8]"Molecular characterization of an autoallergen, Hom s 1, identified by serum IgE from atopic dermatitis patients."
Valenta R., Natter S., Seiberler S., Wichlas S., Maurer D., Hess M., Pavelka M., Grote M., Ferreira F., Szepfalusi Z., Valent P., Stingl G.
J. Invest. Dermatol. 111:1178-1183(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
[9]"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[10]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448 AND SER-591, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP."
Liu S., Rauhut R., Vornlocher H.-P., Luehrmann R.
RNA 12:1418-1430(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[12]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430 AND THR-695, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[13]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-392 AND SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[15]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-430; SER-448 AND THR-764, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[17]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448; SER-521; SER-591; SER-596 AND SER-598, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[18]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[19]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-448, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB006198 mRNA. Translation: BAA24056.1.
AF353625 mRNA. Translation: AAK49523.1.
BT006637 mRNA. Translation: AAP35283.1.
AK223016 mRNA. Translation: BAD96736.1.
AP006287 Genomic DNA. No translation available.
BC001058 mRNA. Translation: AAH01058.1.
AF109680 Genomic DNA. Translation: AAD20223.1.
PIRT00034.
RefSeqNP_005137.1. NM_005146.4.
UniGeneHs.502883.

3D structure databases

ProteinModelPortalO43290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114546. 85 interactions.
DIPDIP-33360N.
IntActO43290. 21 interactions.
MINTMINT-1630512.
STRING9606.ENSP00000310448.

Protein family/group databases

Allergome3322. Hom s 1.0101.
411. Hom s 1.

PTM databases

PhosphoSiteO43290.

Proteomic databases

PaxDbO43290.
PeptideAtlasO43290.
PRIDEO43290.

Protocols and materials databases

DNASU9092.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000312397; ENSP00000310448; ENSG00000175467. [O43290-1]
GeneID9092.
KEGGhsa:9092.
UCSCuc001ogl.3. human. [O43290-1]

Organism-specific databases

CTD9092.
GeneCardsGC11P065730.
H-InvDBHIX0095875.
HGNCHGNC:10538. SART1.
HPAHPA031188.
HPA031189.
HPA031190.
MIM605941. gene.
neXtProtNX_O43290.
PharmGKBPA34947.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG268163.
HOGENOMHOG000046291.
HOVERGENHBG052942.
InParanoidO43290.
KOK11984.
OMAFDDTSEF.
OrthoDBEOG7S7SF7.
PhylomeDBO43290.
TreeFamTF318828.

Gene expression databases

ArrayExpressO43290.
BgeeO43290.
CleanExHS_SART1.
GenevestigatorO43290.

Family and domain databases

InterProIPR005011. SART_1.
[Graphical view]
PANTHERPTHR14152. PTHR14152. 1 hit.
PfamPF03343. SART-1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSART1. human.
GeneWikiSART1.
GenomeRNAi9092.
NextBio34067.
PROO43290.
SOURCESearch...

Entry information

Entry nameSNUT1_HUMAN
AccessionPrimary (citable) accession number: O43290
Secondary accession number(s): A6NDN1, Q53GB5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Allergens

Nomenclature of allergens and list of entries