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Protein

Beta-1,4-galactosyltransferase 5

Gene

B4GALT5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi236 – 2361ManganeseBy similarity
Binding sitei264 – 2641UDP-alpha-D-galactoseBy similarity
Binding sitei296 – 2961UDP-alpha-D-galactoseBy similarity
Metal bindingi329 – 3291Manganese; via tele nitrogenBy similarity
Binding sitei340 – 3401N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.38. 2681.
2.4.1.90. 2681.
ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
R-HSA-913709. O-linked glycosylation of mucins.
R-HSA-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 5 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 5
Short name:
Beta4Gal-T5
Short name:
b4Gal-T5
Alternative name(s):
Beta-1,4-GalT II
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5
Gene namesi
Name:B4GALT5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:928. B4GALT5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414CytoplasmicSequence analysisAdd
BLAST
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini36 – 388353LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25227.

Polymorphism and mutation databases

BioMutaiB4GALT5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Beta-1,4-galactosyltransferase 5PRO_0000080545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence analysis
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence analysis
Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence analysis
Disulfide bondi114 ↔ 158By similarity
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence analysis
Disulfide bondi229 ↔ 248By similarity
Glycosylationi364 – 3641N-linked (GlcNAc...)Sequence analysis
Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO43286.
MaxQBiO43286.
PaxDbiO43286.
PeptideAtlasiO43286.
PRIDEiO43286.

PTM databases

iPTMnetiO43286.
PhosphoSiteiO43286.
SwissPalmiO43286.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiO43286.
CleanExiHS_B4GALT5.
GenevisibleiO43286. HS.

Interactioni

Protein-protein interaction databases

BioGridi114743. 20 interactions.
STRINGi9606.ENSP00000360776.

Structurei

3D structure databases

ProteinModelPortaliO43286.
SMRiO43286. Positions 114-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 1735UDP-alpha-D-galactose bindingBy similarity
Regioni208 – 2103UDP-alpha-D-galactose bindingBy similarity
Regioni235 – 2362UDP-alpha-D-galactose bindingBy similarity
Regioni298 – 3014N-acetyl-D-glucosamine bindingBy similarity
Regioni329 – 3302UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO43286.
KOiK09905.
OMAiVPRWKVA.
OrthoDBiEOG7060R0.
PhylomeDBiO43286.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O43286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMMQAQG
60 70 80 90 100
ILIRDNVRTI GAQVYEQVLR SAYAKRNSSV NDSDYPLDLN HSETFLQTTT
110 120 130 140 150
FLPEDFTYFA NHTCPERLPS MKGPIDINMS EIGMDYIHEL FSKDPTIKLG
160 170 180 190 200
GHWKPSDCMP RWKVAILIPF RNRHEHLPVL FRHLLPMLQR QRLQFAFYVV
210 220 230 240 250
EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE SDRNYYGCGQ
260 270 280 290 300
MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED
310 320 330 340 350
DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER
360 370 380
QGLDGLNNLN YFANITYDAL YKNITVNLTP ELAQVNEY
Length:388
Mass (Da):45,119
Last modified:June 1, 1998 - v1
Checksum:iA71F3F8999B6E13A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti61 – 611G → S.
Corresponds to variant rs2273086 [ dbSNP | Ensembl ].
VAR_024468
Natural varianti368 – 3681D → N.
Corresponds to variant rs235035 [ dbSNP | Ensembl ].
VAR_054022
Natural varianti371 – 3711Y → D.
Corresponds to variant rs35195217 [ dbSNP | Ensembl ].
VAR_033538

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004550 mRNA. Translation: BAA25006.1.
AF038663 mRNA. Translation: AAC39736.1.
AL035683, AL162615 Genomic DNA. Translation: CAI19038.1.
AL162615, AL035683 Genomic DNA. Translation: CAI18968.1.
CH471077 Genomic DNA. Translation: EAW75653.1.
CH471077 Genomic DNA. Translation: EAW75654.1.
BC074821 mRNA. Translation: AAH74821.1.
BC074873 mRNA. Translation: AAH74873.1.
BC104987 mRNA. Translation: AAI04988.1.
BC112265 mRNA. Translation: AAI12266.1.
CCDSiCCDS13420.1.
RefSeqiNP_004767.1. NM_004776.3.
UniGeneiHs.370487.

Genome annotation databases

EnsembliENST00000371711; ENSP00000360776; ENSG00000158470.
GeneIDi9334.
KEGGihsa:9334.
UCSCiuc002xuu.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Beta-1,4-galactosyltransferase 5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004550 mRNA. Translation: BAA25006.1.
AF038663 mRNA. Translation: AAC39736.1.
AL035683, AL162615 Genomic DNA. Translation: CAI19038.1.
AL162615, AL035683 Genomic DNA. Translation: CAI18968.1.
CH471077 Genomic DNA. Translation: EAW75653.1.
CH471077 Genomic DNA. Translation: EAW75654.1.
BC074821 mRNA. Translation: AAH74821.1.
BC074873 mRNA. Translation: AAH74873.1.
BC104987 mRNA. Translation: AAI04988.1.
BC112265 mRNA. Translation: AAI12266.1.
CCDSiCCDS13420.1.
RefSeqiNP_004767.1. NM_004776.3.
UniGeneiHs.370487.

3D structure databases

ProteinModelPortaliO43286.
SMRiO43286. Positions 114-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114743. 20 interactions.
STRINGi9606.ENSP00000360776.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

iPTMnetiO43286.
PhosphoSiteiO43286.
SwissPalmiO43286.

Polymorphism and mutation databases

BioMutaiB4GALT5.

Proteomic databases

EPDiO43286.
MaxQBiO43286.
PaxDbiO43286.
PeptideAtlasiO43286.
PRIDEiO43286.

Protocols and materials databases

DNASUi9334.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371711; ENSP00000360776; ENSG00000158470.
GeneIDi9334.
KEGGihsa:9334.
UCSCiuc002xuu.5. human.

Organism-specific databases

CTDi9334.
GeneCardsiB4GALT5.
HGNCiHGNC:928. B4GALT5.
MIMi604016. gene.
neXtProtiNX_O43286.
PharmGKBiPA25227.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiO43286.
KOiK09905.
OMAiVPRWKVA.
OrthoDBiEOG7060R0.
PhylomeDBiO43286.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.38. 2681.
2.4.1.90. 2681.
ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
R-HSA-913709. O-linked glycosylation of mucins.
R-HSA-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiB4GALT5. human.
GeneWikiiB4GALT5.
GenomeRNAii9334.
PROiO43286.
SOURCEiSearch...

Gene expression databases

BgeeiO43286.
CleanExiHS_B4GALT5.
GenevisibleiO43286. HS.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a human cDNA encoding beta-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1,4-galactosyltransferase."
    Sato T., Furukawa K., Bakker H., van den Eijnden D.H., van Die I.
    Proc. Natl. Acad. Sci. U.S.A. 95:472-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Mammary tumor.
  2. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
    Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
    Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum and Lung.
  6. "Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
    Amado M., Almeida R., Schwientek T., Clausen H.
    Biochim. Biophys. Acta 1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiB4GT5_HUMAN
AccessioniPrimary (citable) accession number: O43286
Secondary accession number(s): E1P625, Q2M394, Q9UJQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 1, 1998
Last modified: July 6, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.