O43286 (B4GT5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,4-galactosyltransferase 5
Short name=Beta-1,4-GalTase 5
Short name=Beta4Gal-T5
Short name=b4Gal-T5
EC=2.4.1.-

Alternative name(s):
Beta-1,4-GalT II
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5

Gene names

Name:

B4GALT5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Ref.1
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Trans cisternae of Golgi stack.
Tissue specificity	Ubiquitously expressed. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	post-translational protein modification Traceable author statement. Source: Reactome protein N-linked glycosylation via asparagine Traceable author statement. Source: Reactome
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Traceable author statement. Source: ProtInc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Beta-1,4-galactosyltransferase 5		PRO_0000080545
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 35	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	36 – 388	353	Lumenal Potential
Sites
	Metal binding	236	1	Manganese By similarity
	Metal binding	329	1	Manganese By similarity
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	128	1	N-linked (GlcNAc...) Potential
	Glycosylation	364	1	N-linked (GlcNAc...) Potential
	Glycosylation	373	1	N-linked (GlcNAc...) Potential
	Disulfide bond	114 ↔ 158		By similarity
	Disulfide bond	229 ↔ 248		By similarity
Natural variations
	Natural variant	61	1	G → S. Corresponds to variant rs2273086 [ dbSNP | Ensembl ].		VAR_024468
	Natural variant	368	1	D → N. Corresponds to variant rs235035 [ dbSNP | Ensembl ].		VAR_054022
	Natural variant	371	1	Y → D. Corresponds to variant rs35195217 [ dbSNP | Ensembl ].		VAR_033538

Sequences

	Sequence		Length	Mass (Da)
	O43286 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: A71F3F8999B6E13A	FASTA	388	45,119
	10 20 30 40 50 60 MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMMQAQG ILIRDNVRTI 70 80 90 100 110 120 GAQVYEQVLR SAYAKRNSSV NDSDYPLDLN HSETFLQTTT FLPEDFTYFA NHTCPERLPS 130 140 150 160 170 180 MKGPIDINMS EIGMDYIHEL FSKDPTIKLG GHWKPSDCMP RWKVAILIPF RNRHEHLPVL 190 200 210 220 230 240 FRHLLPMLQR QRLQFAFYVV EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE 250 260 270 280 290 300 SDRNYYGCGQ MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED 310 320 330 340 350 360 DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER QGLDGLNNLN 370 380 YFANITYDAL YKNITVNLTP ELAQVNEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a human cDNA encoding beta-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1,4-galactosyltransferase." Sato T., Furukawa K., Bakker H., van den Eijnden D.H., van Die I. Proc. Natl. Acad. Sci. U.S.A. 95:472-477(1998) [PubMed: 9435216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Mammary tumor.
[2]	"The expanding beta 4-galactosyltransferase gene family: messages from the databanks." Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L. Glycobiology 8:517-526(1998) [PubMed: 9597550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum and Lung.
[6]	"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions." Amado M., Almeida R., Schwientek T., Clausen H. Biochim. Biophys. Acta 1473:35-53(1999) [PubMed: 10580128] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,4-galactosyltransferase 5

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB004550 mRNA. Translation: BAA25006.1. AF038663 mRNA. Translation: AAC39736.1. AL035683, AL162615 Genomic DNA. Translation: CAI19038.1. AL162615, AL035683 Genomic DNA. Translation: CAI18968.1. CH471077 Genomic DNA. Translation: EAW75653.1. CH471077 Genomic DNA. Translation: EAW75654.1. BC074821 mRNA. Translation: AAH74821.1. BC074873 mRNA. Translation: AAH74873.1. BC104987 mRNA. Translation: AAI04988.1. BC112265 mRNA. Translation: AAI12266.1.
IPI	IPI00011656.
RefSeq	NP_004767.1. NM_004776.3.
UniGene	Hs.370487.
3D structure databases
ProteinModelPortal	O43286.
SMR	O43286. Positions 111-381.
ModBase	Search...
Protein-protein interaction databases
STRING	O43286.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	O43286.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371711; ENSP00000360776; ENSG00000158470.
GeneID	9334.
KEGG	hsa:9334.
UCSC	uc002xuu.2. human.
Organism-specific databases
CTD	9334.
GeneCards	GC20M048249.
H-InvDB	HIX0015903.
HGNC	HGNC:928. B4GALT5.
MIM	604016. gene.
neXtProt	NX_O43286.
PharmGKB	PA25227.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04392.
HOGENOM	HBG717407.
HOVERGEN	HBG058334.
InParanoid	O43286.
OMA	PSMKGPI.
OrthoDB	EOG41RPV2.
PhylomeDB	O43286.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	O43286.
Bgee	O43286.
CleanEx	HS_B4GALT5.
Genevestigator	O43286.
GermOnline	ENSG00000158470. Homo sapiens.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2_met. [Graphical view]
KO	K09905.
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
Pfam	PF02709. Galactosyl_T_2. 1 hit. [Graphical view]
PRINTS	PR02050. B14GALTRFASE.
ProtoNet	Search...
Other
NextBio	34967.
SOURCE	Search...

Entry information

Entry name

B4GT5_HUMAN

Accession

Primary (citable) accession number: O43286
Secondary accession number(s): E1P625, Q2M394, Q9UJQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.