O43280 (TREA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trehalase
EC=3.2.1.28

Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase

Gene names

Name:	TREH
Synonyms:	TREA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Intestinal trehalase is probably involved in the hydrolysis of ingested trehalose.
Catalytic activity	Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Involvement in disease	Deficiency of TREH results in isolated trehalose intolerance that causes gastrointestinal symptoms after ingestion of edible mushrooms.
Sequence similarities	Belongs to the glycosyl hydrolase 37 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	polysaccharide digestion Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome trehalose catabolic process Traceable author statement Ref.1. Source: ProtInc
Cellular_component	anchored to plasma membrane Traceable author statement Ref.5. Source: UniProtKB
Molecular_function	alpha,alpha-trehalase activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43280-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43280-2)

The sequence of this isoform differs from the canonical sequence as follows:
     175-206: WDSYWVMEGLLLSEMAETVKGMLQNFLDLVKT → C

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 556	533	Trehalase		PRO_0000012051
	Propeptide	557 – 583	27	Removed in mature form By similarity		PRO_0000012052
Amino acid modifications
	Lipidation	556	1	GPI-anchor amidated serine Potential
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	239	1	N-linked (GlcNAc...) Potential
	Glycosylation	261	1	N-linked (GlcNAc...) Potential
	Glycosylation	369	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	175 – 206	32	WDSYW…DLVKT → C in isoform 2.		VSP_035440
	Natural variant	389	1	T → A. Corresponds to variant rs2276065 [ dbSNP | Ensembl ].		VAR_049205
	Natural variant	449	1	Y → H. Corresponds to variant rs11827611 [ dbSNP | Ensembl ].		VAR_049206
	Natural variant	486	1	R → W. Corresponds to variant rs2276064 [ dbSNP | Ensembl ].		VAR_049207
	Natural variant	558	1	A → P. Corresponds to variant rs6589671 [ dbSNP | Ensembl ].		VAR_049208
	Natural variant	561	1	A → P. Corresponds to variant rs6589670 [ dbSNP | Ensembl ].		VAR_061191
Experimental info
	Sequence conflict	539 – 540	2	TN → DE in BAA24381. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: 3EE4D312B82185F9	FASTA	583	66,568
	10 20 30 40 50 60 MPGRTWELCL LLLLGLGLGS QEALPPPCES EIYCHGELLN QVQMAKLYQD DKQFVDMPLS 70 80 90 100 110 120 IAPEQVLQTF TELSRDHNHS IPREQLQAFV HEHFQAKGQE LQPWTPADWK DSPQFLQKIS 130 140 150 160 170 180 DAKLRAWAGQ LHQLWKKLGK KMKPEVLSHP ERFSLIYSEH PFIVPGGRFV EFYYWDSYWV 190 200 210 220 230 240 MEGLLLSEMA ETVKGMLQNF LDLVKTYGHV PNGGRVYYLQ RSQPPLLTLM MDCYLTHTND 250 260 270 280 290 300 TAFLQENIET LALELDFWTK NRTVSVSLEG KNYLLNRYYV PYGGPRPESY SKDVELADTL 310 320 330 340 350 360 PEGDREALWA ELKAGAESGW DFSSRWLIGG PNPNSLSGIR TSKLVPVDLN AFLCQAEELM 370 380 390 400 410 420 SNFYSRLGND SQATKYRILR SQRLAALNTV LWDEQTGAWF DYDLEKKKKN REFYPSNLTP 430 440 450 460 470 480 LWAGCFSDPG VADKALKYLE DNRILTYQYG IPTSLQKTGQ QWDFPNAWAP LQDLVIRGLA 490 500 510 520 530 540 KAPLRRAQEV AFQLAQNWIR TNFDVYSQKS AMYEKYDVSN GGQPGGGGEY EVQEGFGWTN 550 560 570 580 GVVLMLLDRY GDRLTSGAKL AFLEPHCLAA TLLPSLLLSL LPW
	Isoform 2 [UniParc]. Checksum: 09B87A62D49F49DF	FASTA	552	62,941
	10 20 30 40 50 60 MPGRTWELCL LLLLGLGLGS QEALPPPCES EIYCHGELLN QVQMAKLYQD DKQFVDMPLS 70 80 90 100 110 120 IAPEQVLQTF TELSRDHNHS IPREQLQAFV HEHFQAKGQE LQPWTPADWK DSPQFLQKIS 130 140 150 160 170 180 DAKLRAWAGQ LHQLWKKLGK KMKPEVLSHP ERFSLIYSEH PFIVPGGRFV EFYYCYGHVP 190 200 210 220 230 240 NGGRVYYLQR SQPPLLTLMM DCYLTHTNDT AFLQENIETL ALELDFWTKN RTVSVSLEGK 250 260 270 280 290 300 NYLLNRYYVP YGGPRPESYS KDVELADTLP EGDREALWAE LKAGAESGWD FSSRWLIGGP 310 320 330 340 350 360 NPNSLSGIRT SKLVPVDLNA FLCQAEELMS NFYSRLGNDS QATKYRILRS QRLAALNTVL 370 380 390 400 410 420 WDEQTGAWFD YDLEKKKKNR EFYPSNLTPL WAGCFSDPGV ADKALKYLED NRILTYQYGI 430 440 450 460 470 480 PTSLQKTGQQ WDFPNAWAPL QDLVIRGLAK APLRRAQEVA FQLAQNWIRT NFDVYSQKSA 490 500 510 520 530 540 MYEKYDVSNG GQPGGGGEYE VQEGFGWTNG VVLMLLDRYG DRLTSGAKLA FLEPHCLAAT 550 LLPSLLLSLL PW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, sequencing and expression of cDNA encoding human trehalase." Ishihara R., Taketani S., Sasai-Takedatsu M., Kino M., Tokunaga R., Kobayashi Y. Gene 202:69-74(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Kidney.
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"Human trehalase: characterization, localization, and its increase in urine by renal proximal tubular damage." Sasai-Takedatsu M., Taketani S., Nagata N., Furukawa T., Tokunaga R., Kojima T., Kobayashi Y. Nephron 73:179-185(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Web resources

Wikipedia

Trehalase entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB000824 mRNA. Translation: BAA24381.1. AK223140 mRNA. Translation: BAD96860.1. AK223143 mRNA. Translation: BAD96863.1. CH471065 Genomic DNA. Translation: EAW67409.1. BC109206 mRNA. Translation: AAI09207.1.
IPI	IPI00011650. IPI00656107.
PIR	JC6504.
RefSeq	NP_009111.2. NM_007180.2.
UniGene	Hs.129712.
3D structure databases
ProteinModelPortal	O43280.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000264029.
Protein family/group databases
CAZy	GH37. Glycoside Hydrolase Family 37.
PTM databases
PhosphoSite	O43280.
Proteomic databases
PaxDb	O43280.
PRIDE	O43280.
Protocols and materials databases
DNASU	11181.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264029; ENSP00000264029; ENSG00000118094.
GeneID	11181.
KEGG	hsa:11181.
UCSC	uc001pty.1. human.
Organism-specific databases
CTD	11181.
GeneCards	GC11M118528.
H-InvDB	HIX0010188.
HGNC	HGNC:12266. TREH.
HPA	HPA039913.
MIM	275360. gene.
neXtProt	NX_O43280.
Orphanet	103909. Diarrhea-vomiting due to trehalase deficiency.
PharmGKB	PA36946.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1626.
HOGENOM	HOG000215465.
HOVERGEN	HBG014956.
InParanoid	O43280.
KO	K01194.
OrthoDB	EOG4R23TH.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	O43280.
Bgee	O43280.
CleanEx	HS_TREH.
Genevestigator	O43280.
GermOnline	ENSG00000118094. Homo sapiens.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR001661. Glyco_hydro_37. IPR018232. Glyco_hydro_37_CS. [Graphical view]
PANTHER	PTHR23403. PTHR23403. 1 hit.
Pfam	PF01204. Trehalase. 1 hit. [Graphical view]
PRINTS	PR00744. GLHYDRLASE37.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00927. TREHALASE_1. 1 hit. PS00928. TREHALASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O43280.
ChEMBL	CHEMBL3087.
GenomeRNAi	11181.
NextBio	42547.
SOURCE	Search...

Entry information

Entry name

TREA_HUMAN

Accession

Primary (citable) accession number: O43280
Secondary accession number(s): Q32MB9, Q53FY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.