O43264 (ZW10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere/kinetochore protein zw10 homolog

Gene names

Name:

ZW10

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum. Ref.4 Ref.6 Ref.8 Ref.9 Ref.10
Subunit structure	Associated with a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with RINT1/TIP20L bound to BNIP1/SEC20L. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts with C19orf25, KNTC1, and ZWINT. Ref.5 Ref.6 Ref.7 Ref.9 Ref.10 Ref.11
Subcellular location	Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Chromosome › centromere › kinetochore. Cytoplasm › cytoskeleton › spindle. Note: Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm. In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone. Ref.4 Ref.6 Ref.9 Ref.10 Ref.11
Tissue specificity	Widely expressed.
Developmental stage	No significant variation in expression during cell cycle.
Miscellaneous	Overexpression as well as silencing of ZW10 disrupts the morphology of the ER-Golgi intermediate compartment as well as the Golgi apparatus and slows down ER-Golgi transport.
Sequence similarities	Belongs to the ZW10 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division ER-Golgi transport Mitosis Protein transport Transport
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Endoplasmic reticulum Kinetochore Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from mutant phenotype Ref.6. Source: HGNC cell division Inferred from electronic annotation. Source: UniProtKB-KW establishment of mitotic spindle orientation Inferred from mutant phenotype. Source: UniProtKB meiosis Traceable author statement. Source: ProtInc mitotic cell cycle checkpoint Inferred from direct assay. Source: UniProtKB mitotic metaphase plate congression Inferred from mutant phenotype. Source: UniProtKB mitotic prometaphase Traceable author statement. Source: Reactome protein complex assembly Inferred from direct assay. Source: HGNC protein localization to kinetochore Inferred from mutant phenotype. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of exit from mitosis Inferred from direct assay. Source: HGNC
Cellular component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell kinetochore microtubule Inferred from direct assay. Source: UniProtKB nucleus Inferred from direct assay Ref.9. Source: HGNC spindle pole Inferred from direct assay. Source: UniProtKB
Molecular function	centromeric DNA binding Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ZWINT	O95229	6	EBI-1001217,EBI-1001132

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2 Ref.3
	Chain	2 – 779	778	Centromere/kinetochore protein zw10 homolog		PRO_0000184957
Regions
	Region	2 – 317	316	Interaction with RINT1
	Region	2 – 81	80	Interaction with ZWINT
	Coiled coil	14 – 130	117	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.3 Ref.13
	Modified residue	438	1	Phosphothreonine Ref.12
	Modified residue	777	1	N6-acetyllysine Ref.14
Natural variations
	Natural variant	77	1	I → M. Corresponds to variant rs2271796 [ dbSNP | Ensembl ].		VAR_053537

Sequences

	Sequence		Length	Mass (Da)
	O43264 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9C38186153886481	FASTA	779	88,829
	10 20 30 40 50 60 MASFVTEVLA HSGRLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL PSMQSAQGLI 70 80 90 100 110 120 TQVDKLSEDI DLLKSRIESE VRRDLHVSTG EFTDLKQQLE RDSVVLSLLK QLQEFSTAIE 130 140 150 160 170 180 EYNCALTEKK YVTGAQRLEE AQKCLKLLKS RKCFDLKILK SLSMELTIQK QNILYHLGEE 190 200 210 220 230 240 WQKLIVWKFP PSKDTSSLES YLQTELHLYT EQSHKEEKTP MPPISSVLLA FSVLGELHSK 250 260 270 280 290 300 LKSFGQMLLK YILRPLASCP SLHAVIESQP NIVIIRFESI MTNLEYPSPS EVFTKIRLVL 310 320 330 340 350 360 EVLQKQLLDL PLDTDLENEK TSTVPLAEML GDMIWEDLSE CLIKNCLVYS IPTNSSKLQQ 370 380 390 400 410 420 YEEIIQSTEE FENALKEMRF LKGDTTDLLK YARNINSHFA NKKCQDVIVA ARNLMTSEIH 430 440 450 460 470 480 NTVKIIPDSK INVPELPTPD EDNKLEVQKV SNTQYHEVMN LEPENTLDQH SFSLPTCRIS 490 500 510 520 530 540 ESVKKLMELA YQTLLEATTS SDQCAVQLFY SVRNIFHLFH DVVPTYHKEN LQKLPQLAAI 550 560 570 580 590 600 HHNNCMYIAH HLLTLGHQFR LRLAPILCDG TATFVDLVPG FRRLGTECFL AQMRAQKGEL 610 620 630 640 650 660 LERLSSARNF SNMDDEENYS AASKAVRQVL HQLKRLGIVW QDVLPVNIYC KAMGTLLNTA 670 680 690 700 710 720 ISEVIGKITA LEDISTEDGD RLYSLCKTVM DEGPQVFAPL SEESKNKKYQ EEVPVYVPKW 730 740 750 760 770 MPFKELMMML QASLQEIGDR WADGKGPLAA AFSSSEVKAL IRALFQNTER RAAALAKIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conservation of the centromere/kinetochore protein ZW10." Starr D.A., Williams B.C., Li Z., Etemad-Moghadam B., Dawe R.K., Goldberg M.L. J. Cell Biol. 138:1289-1301(1997) [PubMed: 9298984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cervix carcinoma.
[2]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14. Tissue: Platelet.
[3]	Bienvenut W.V., Boldt K., von Kriegsheim A.F., Kolch W. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-14 AND 161-170, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Hepatoma.
[4]	"The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore." Scaeerou F., Starr D.A., Piano F., Papoulas O., Karess R.E., Goldberg M.L. J. Cell Sci. 114:3103-3114(2001) [PubMed: 11590237] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions." Williams B.C., Li Z., Liu S., Williams E.V., Leung G., Yen T.J., Goldberg M.L. Mol. Biol. Cell 14:1379-1391(2003) [PubMed: 12686595] [Abstract] Cited for: INTERACTION WITH ZWILCH AND ZW10.
[6]	"Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi." Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M., Tani K., Yamamoto A., Tohyama M., Tagaya M. EMBO J. 23:1267-1278(2004) [PubMed: 15029241] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH STX18; USE1L; SEC22B AND RINT1, INTERACTION WITH RINT1.
[7]	"Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion." Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K., Nagahama M., Tani K., Yamamoto A., Tagaya M. EMBO J. 23:3216-3226(2004) [PubMed: 15272311] [Abstract] Cited for: INTERACTION WITH BNIP1 THROUGH RINT1.
[8]	"Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes." Musio A., Mariani T., Montagna C., Zambroni D., Ascoli C., Ried T., Vezzoni P. Gene 331:33-40(2004) [PubMed: 15094189] [Abstract] Cited for: FUNCTION.
[9]	"Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling." Wang H., Hu X., Ding X., Dou Z., Yang Z., Shaw A.W., Teng M., Cleveland D.W., Goldberg M.L., Niu L., Yao X. J. Biol. Chem. 279:54590-54598(2004) [PubMed: 15485811] [Abstract] Cited for: FUNCTION, INTERACTION WITH ZWINT, SUBCELLULAR LOCATION.
[10]	"ZW10 links mitotic checkpoint signaling to the structural kinetochore." Kops G.J.P.L., Kim Y., Weaver B.A.A., Mao Y., McLeod I., Yates J.R. III, Tagaya M., Cleveland D.W. J. Cell Biol. 169:49-60(2005) [PubMed: 15824131] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH C19ORF25; KNTC1; RINT1; ZWILCH AND ZWINT, SUBCELLULAR LOCATION.
[11]	"Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control." Lin Y.-T., Chen Y., Wu G., Lee W.-H. Oncogene 25:6901-6914(2006) [PubMed: 16732327] [Abstract] Cited for: INTERACTION WITH ZWINT, SUBCELLULAR LOCATION.
[12]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-438, MASS SPECTROMETRY. Tissue: Cervix adenocarcinoma.
[13]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[14]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-777, MASS SPECTROMETRY.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U54996 mRNA. Translation: AAB88237.1.
IPI	IPI00011631.
RefSeq	NP_004715.1. NM_004724.3.
UniGene	Hs.503886.
3D structure databases
ProteinModelPortal	O43264.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-36471N.
IntAct	O43264. 13 interactions.
STRING	O43264.
PTM databases
PhosphoSite	O43264.
Proteomic databases
PeptideAtlas	O43264.
PRIDE	O43264.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000200135; ENSP00000200135; ENSG00000086827.
GeneID	9183.
KEGG	hsa:9183.
UCSC	uc001poe.1. human.
Organism-specific databases
CTD	9183.
GeneCards	GC11M113603.
H-InvDB	HIX0036029.
HGNC	HGNC:13194. ZW10.
HPA	CAB011565.
MIM	603954. gene.
neXtProt	NX_O43264.
PharmGKB	PA37759.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000016427.
HOGENOM	HBG715812.
HOVERGEN	HBG004603.
InParanoid	O43264.
OMA	EHPSPSE.
OrthoDB	EOG4Q84WT.
PhylomeDB	O43264.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_383. DNA Replication.
Gene expression databases
ArrayExpress	O43264.
Bgee	O43264.
CleanEx	HS_ZW10.
Genevestigator	O43264.
GermOnline	ENSG00000086827. Homo sapiens.
Family and domain databases
InterPro	IPR009361. RZZ-complex_Zw10. [Graphical view]
KO	K11578.
Pfam	PF06248. Zw10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	34429.
SOURCE	Search...

Entry information

Entry name

ZW10_HUMAN

Accession

Primary (citable) accession number: O43264

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.