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O43257

- ZNHI1_HUMAN

UniProt

O43257 - ZNHI1_HUMAN

Protein

Zinc finger HIT domain-containing protein 1

Gene

ZNHIT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Seems to play a role in p53-mediated apoptosis induction. Binds to NR1D2 and relieves it of its inhibitory effect on the transcription of APOC3 without affecting its DNA-binding activity.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri117 – 14933HIT-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. negative regulation of G0 to G1 transition Source: Ensembl
    2. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    3. regulation of histone deacetylation Source: Ensembl
    4. regulation of T cell proliferation Source: Ensembl

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger HIT domain-containing protein 1
    Alternative name(s):
    Cyclin-G1-binding protein 1
    Zinc finger protein subfamily 4A member 1
    p18 Hamlet
    Gene namesi
    Name:ZNHIT1
    Synonyms:CGBP1, ZNFN4A1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:21688. ZNHIT1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi103 – 1031T → A: Impairs the p38 MAPK-mediated phosphorylation of ZNHIT1. 1 Publication
    Mutagenesisi124 – 1241S → A: No change in the in vitro MAPK14/MAPK11-induced phosphorylation level of ZNHIT1.

    Organism-specific databases

    PharmGKBiPA134947251.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 154154Zinc finger HIT domain-containing protein 1PRO_0000173546Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei103 – 1031Phosphothreonine; by MAPK11 and MAPK141 Publication

    Post-translational modificationi

    Phosphorylated on Thr by MAPK11 or MAPK14.1 Publication
    Stres-induced ZNHIT1 is mainly regulated at the level of protein.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO43257.
    PaxDbiO43257.
    PRIDEiO43257.

    PTM databases

    PhosphoSiteiO43257.

    Expressioni

    Tissue specificityi

    Expressed abundantly in liver, but weakly in skeletal muscle, ovary and small intestine.1 Publication

    Inductioni

    Induced by DNA damage.1 Publication

    Gene expression databases

    BgeeiO43257.
    CleanExiHS_ZNHIT1.
    GenevestigatoriO43257.

    Organism-specific databases

    HPAiHPA019043.

    Interactioni

    Subunit structurei

    Interacts with MAPK11 and MAPK14. Component of the chromatin-remodeling SRCAP complex composed of at least SRCAP, DMAP1, RUVBL1, RUVBL2, ACTL6A, YEATS4, ACTR6 and ZNHIT1. Interacts with NR1D1 and NR2D2.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACTR6Q9GZN14EBI-347522,EBI-769329
    H2AFZP0C0S53EBI-347522,EBI-1199859
    MAPK11Q157592EBI-347522,EBI-298304
    MAPK14Q165397EBI-347522,EBI-73946
    TP53BP1Q128882EBI-347522,EBI-396540

    Protein-protein interaction databases

    BioGridi115730. 7 interactions.
    IntActiO43257. 16 interactions.
    MINTiMINT-1036481.
    STRINGi9606.ENSP00000304593.

    Structurei

    3D structure databases

    ProteinModelPortaliO43257.
    SMRiO43257. Positions 115-146.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni72 – 11039Interaction with NR1D2Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili23 – 3917Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi38 – 4710Nuclear localization signal

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi66 – 705Poly-Lys

    Sequence similaritiesi

    Belongs to the ZNHIT1 family.Curated
    Contains 1 HIT-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri117 – 14933HIT-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG282558.
    HOGENOMiHOG000264917.
    HOVERGENiHBG054274.
    InParanoidiO43257.
    KOiK11663.
    OMAiACGTRYC.
    OrthoDBiEOG722JBH.
    PhylomeDBiO43257.
    TreeFamiTF314330.

    Family and domain databases

    InterProiIPR007529. Znf_HIT.
    [Graphical view]
    PfamiPF04438. zf-HIT. 1 hit.
    [Graphical view]
    PROSITEiPS51083. ZF_HIT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O43257-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEKKTSVRS QDPGQRRVLD RAARQRRINR QLEALENDNF QDDPHAGLPQ    50
    LGKRLPQFDD DADTGKKKKK TRGDHFKLRF RKNFQALLEE QNLSVAEGPN 100
    YLTACAGPPS RPQRPFCAVC GFPSPYTCVS CGARYCTVRC LGTHQETRCL 150
    KWTV 154
    Length:154
    Mass (Da):17,536
    Last modified:June 1, 1998 - v1
    Checksum:i6A45F05249DC07A6
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti134 – 1341R → W in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_035719

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093571 mRNA. Translation: AAF03558.1.
    U61837 mRNA. Translation: AAB88578.1.
    CR456992 mRNA. Translation: CAG33273.1.
    AC004876 Genomic DNA. Translation: AAD45833.1.
    CH471197 Genomic DNA. Translation: EAW50209.1.
    BC017333 mRNA. Translation: AAH17333.1.
    CCDSiCCDS5716.1.
    RefSeqiNP_006340.1. NM_006349.2.
    UniGeneiHs.211079.

    Genome annotation databases

    EnsembliENST00000305105; ENSP00000304593; ENSG00000106400.
    GeneIDi10467.
    KEGGihsa:10467.
    UCSCiuc003uye.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093571 mRNA. Translation: AAF03558.1 .
    U61837 mRNA. Translation: AAB88578.1 .
    CR456992 mRNA. Translation: CAG33273.1 .
    AC004876 Genomic DNA. Translation: AAD45833.1 .
    CH471197 Genomic DNA. Translation: EAW50209.1 .
    BC017333 mRNA. Translation: AAH17333.1 .
    CCDSi CCDS5716.1.
    RefSeqi NP_006340.1. NM_006349.2.
    UniGenei Hs.211079.

    3D structure databases

    ProteinModelPortali O43257.
    SMRi O43257. Positions 115-146.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115730. 7 interactions.
    IntActi O43257. 16 interactions.
    MINTi MINT-1036481.
    STRINGi 9606.ENSP00000304593.

    PTM databases

    PhosphoSitei O43257.

    Proteomic databases

    MaxQBi O43257.
    PaxDbi O43257.
    PRIDEi O43257.

    Protocols and materials databases

    DNASUi 10467.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000305105 ; ENSP00000304593 ; ENSG00000106400 .
    GeneIDi 10467.
    KEGGi hsa:10467.
    UCSCi uc003uye.3. human.

    Organism-specific databases

    CTDi 10467.
    GeneCardsi GC07P100860.
    HGNCi HGNC:21688. ZNHIT1.
    HPAi HPA019043.
    neXtProti NX_O43257.
    PharmGKBi PA134947251.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG282558.
    HOGENOMi HOG000264917.
    HOVERGENi HBG054274.
    InParanoidi O43257.
    KOi K11663.
    OMAi ACGTRYC.
    OrthoDBi EOG722JBH.
    PhylomeDBi O43257.
    TreeFami TF314330.

    Miscellaneous databases

    ChiTaRSi ZNHIT1. human.
    GenomeRNAii 10467.
    NextBioi 39695.
    PROi O43257.

    Gene expression databases

    Bgeei O43257.
    CleanExi HS_ZNHIT1.
    Genevestigatori O43257.

    Family and domain databases

    InterProi IPR007529. Znf_HIT.
    [Graphical view ]
    Pfami PF04438. zf-HIT. 1 hit.
    [Graphical view ]
    PROSITEi PS51083. ZF_HIT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of cyclin G1 binding protein 1 (CGBP1)."
      Wang Y., Underwood L.J., Tanimoto H., Shigemasa K., O'Brien T.J.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Xu F., Hall F.L., Starnes V., Wu L.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    7. "The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes."
      Cai Y., Jin J., Florens L., Swanson S.K., Kusch T., Li B., Workman J.L., Washburn M.P., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 280:13665-13670(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SRCAP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "A new p38 MAP kinase-regulated transcriptional coactivator that stimulates p53-dependent apoptosis."
      Cuadrado A., Lafarga V., Cheung P.C., Dolado I., Llanos S., Cohen P., Nebreda A.R.
      EMBO J. 26:2115-2126(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAPK11 AND MAPK14, PHOSPHORYLATION AT THR-103 BY MAPK11 AND MAPK14, INDUCTION BY DNA DAMAGE, FUNCTION, MUTAGENESIS OF THR-103.
    9. "A zinc finger HIT domain-containing protein, ZNHIT-1, interacts with orphan nuclear hormone receptor Rev-erbbeta and removes Rev-erbbeta-induced inhibition of apoCIII transcription."
      Wang J., Li Y., Zhang M., Liu Z., Wu C., Yuan H., Li Y.Y., Zhao X., Lu H.
      FEBS J. 274:5370-5381(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NR1D1 AND NR1D2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: VARIANT [LARGE SCALE ANALYSIS] TRP-134.

    Entry informationi

    Entry nameiZNHI1_HUMAN
    AccessioniPrimary (citable) accession number: O43257
    Secondary accession number(s): Q6IB12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3