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O43257 (ZNHI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger HIT domain-containing protein 1
Alternative name(s):
Cyclin-G1-binding protein 1
Zinc finger protein subfamily 4A member 1
p18 Hamlet
Gene names
Name:ZNHIT1
Synonyms:CGBP1, ZNFN4A1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length154 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to play a role in p53-mediated apoptosis induction. Binds to NR1D2 and relieves it of its inhibitory effect on the transcription of APOC3 without affecting its DNA-binding activity. Ref.8 Ref.9

Subunit structure

Interacts with MAPK11 and MAPK14. Component of the chromatin-remodeling SRCAP complex composed of at least SRCAP, DMAP1, RUVBL1, RUVBL2, ACTL6A, YEATS4, ACTR6 and ZNHIT1. Interacts with NR1D1 and NR2D2. Ref.7 Ref.8 Ref.9

Subcellular location

Nucleus Ref.9.

Tissue specificity

Expressed abundantly in liver, but weakly in skeletal muscle, ovary and small intestine. Ref.9

Induction

Induced by DNA damage. Ref.8

Post-translational modification

Phosphorylated on Thr by MAPK11 or MAPK14. Ref.8

Stres-induced ZNHIT1 is mainly regulated at the level of protein.

Sequence similarities

Belongs to the ZNHIT1 family.

Contains 1 HIT-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 154154Zinc finger HIT domain-containing protein 1
PRO_0000173546

Regions

Zinc finger117 – 14933HIT-type
Region72 – 11039Interaction with NR1D2
Coiled coil23 – 3917 Potential
Motif38 – 4710Nuclear localization signal
Compositional bias66 – 705Poly-Lys

Amino acid modifications

Modified residue1031Phosphothreonine; by MAPK11 and MAPK14 Ref.8

Natural variations

Natural variant1341R → W in a colorectal cancer sample; somatic mutation. Ref.11
VAR_035719

Experimental info

Mutagenesis1031T → A: Impairs the p38 MAPK-mediated phosphorylation of ZNHIT1. Ref.8
Mutagenesis1241S → A: No change in the in vitro MAPK14/MAPK11-induced phosphorylation level of ZNHIT1.

Sequences

Sequence LengthMass (Da)Tools
O43257 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 6A45F05249DC07A6

FASTA15417,536
        10         20         30         40         50         60 
MVEKKTSVRS QDPGQRRVLD RAARQRRINR QLEALENDNF QDDPHAGLPQ LGKRLPQFDD 

        70         80         90        100        110        120 
DADTGKKKKK TRGDHFKLRF RKNFQALLEE QNLSVAEGPN YLTACAGPPS RPQRPFCAVC 

       130        140        150 
GFPSPYTCVS CGARYCTVRC LGTHQETRCL KWTV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of cyclin G1 binding protein 1 (CGBP1)."
Wang Y., Underwood L.J., Tanimoto H., Shigemasa K., O'Brien T.J.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Xu F., Hall F.L., Starnes V., Wu L.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[7]"The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes."
Cai Y., Jin J., Florens L., Swanson S.K., Kusch T., Li B., Workman J.L., Washburn M.P., Conaway R.C., Conaway J.W.
J. Biol. Chem. 280:13665-13670(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SRCAP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"A new p38 MAP kinase-regulated transcriptional coactivator that stimulates p53-dependent apoptosis."
Cuadrado A., Lafarga V., Cheung P.C., Dolado I., Llanos S., Cohen P., Nebreda A.R.
EMBO J. 26:2115-2126(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAPK11 AND MAPK14, PHOSPHORYLATION AT THR-103 BY MAPK11 AND MAPK14, INDUCTION BY DNA DAMAGE, FUNCTION, MUTAGENESIS OF THR-103.
[9]"A zinc finger HIT domain-containing protein, ZNHIT-1, interacts with orphan nuclear hormone receptor Rev-erbbeta and removes Rev-erbbeta-induced inhibition of apoCIII transcription."
Wang J., Li Y., Zhang M., Liu Z., Wu C., Yuan H., Li Y.Y., Zhao X., Lu H.
FEBS J. 274:5370-5381(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NR1D1 AND NR1D2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] TRP-134.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF093571 mRNA. Translation: AAF03558.1.
U61837 mRNA. Translation: AAB88578.1.
CR456992 mRNA. Translation: CAG33273.1.
AC004876 Genomic DNA. Translation: AAD45833.1.
CH471197 Genomic DNA. Translation: EAW50209.1.
BC017333 mRNA. Translation: AAH17333.1.
CCDSCCDS5716.1.
RefSeqNP_006340.1. NM_006349.2.
UniGeneHs.211079.

3D structure databases

ProteinModelPortalO43257.
SMRO43257. Positions 115-146.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115730. 7 interactions.
IntActO43257. 16 interactions.
MINTMINT-1036481.
STRING9606.ENSP00000304593.

PTM databases

PhosphoSiteO43257.

Proteomic databases

MaxQBO43257.
PaxDbO43257.
PRIDEO43257.

Protocols and materials databases

DNASU10467.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000305105; ENSP00000304593; ENSG00000106400.
GeneID10467.
KEGGhsa:10467.
UCSCuc003uye.3. human.

Organism-specific databases

CTD10467.
GeneCardsGC07P100860.
HGNCHGNC:21688. ZNHIT1.
HPAHPA019043.
neXtProtNX_O43257.
PharmGKBPA134947251.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG282558.
HOGENOMHOG000264917.
HOVERGENHBG054274.
InParanoidO43257.
KOK11663.
OMAACGTRYC.
OrthoDBEOG722JBH.
PhylomeDBO43257.
TreeFamTF314330.

Gene expression databases

BgeeO43257.
CleanExHS_ZNHIT1.
GenevestigatorO43257.

Family and domain databases

InterProIPR007529. Znf_HIT.
[Graphical view]
PfamPF04438. zf-HIT. 1 hit.
[Graphical view]
PROSITEPS51083. ZF_HIT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZNHIT1. human.
GenomeRNAi10467.
NextBio39695.
PROO43257.

Entry information

Entry nameZNHI1_HUMAN
AccessionPrimary (citable) accession number: O43257
Secondary accession number(s): Q6IB12
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM