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Protein

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1

Gene

PAPSS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Enzyme regulationi

Inhibited by chlorate.

Pathwayi: sulfate assimilation

This protein is involved in the pathway sulfate assimilation, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway sulfate assimilation and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133Phosphoserine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 66ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS06566-MONOMER.
ZFISH:HS06566-MONOMER.
BRENDAi2.7.1.25. 2681.
2.7.7.4. 2681.
ReactomeiR-HSA-174362. Transport and synthesis of PAPS.
R-HSA-2408550. Metabolism of ingested H2SeO4 and H2SeO3 into H2Se.
R-HSA-6802952. Signaling by BRAF and RAF fusions.
SABIO-RKO43252.
UniPathwayiUPA00097.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Short name:
PAPS synthase 1
Short name:
PAPSS 1
Alternative name(s):
Sulfurylase kinase 1
Short name:
SK 1
Short name:
SK1
Including the following 2 domains:
Sulfate adenylyltransferase (EC:2.7.7.4)
Alternative name(s):
ATP-sulfurylase
Sulfate adenylate transferase
Short name:
SAT
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate synthase
APS kinase
Adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenylylsulfate 3'-phosphotransferase
Gene namesi
Name:PAPSS1
Synonyms:ATPSK1, PAPSS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:8603. PAPSS1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi425H → A: Loss of activity. 1 Publication1
Mutagenesisi426N → K: Increased activity. 1 Publication1
Mutagenesisi427 – 428GH → AA: Loss of activity. 2
Mutagenesisi427G → A: 30% decrease in activity. 1 Publication1
Mutagenesisi428H → A: Loss of activity. 1 Publication1

Organism-specific databases

DisGeNETi9061.
OpenTargetsiENSG00000138801.
PharmGKBiPA384.

Polymorphism and mutation databases

BioMutaiPAPSS1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001059591 – 624Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1Add BLAST624

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei12N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO43252.
PaxDbiO43252.
PeptideAtlasiO43252.
PRIDEiO43252.

PTM databases

iPTMnetiO43252.
PhosphoSitePlusiO43252.

Expressioni

Tissue specificityi

Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage.

Gene expression databases

BgeeiENSG00000138801.
CleanExiHS_PAPSS1.
ExpressionAtlasiO43252. baseline and differential.
GenevisibleiO43252. HS.

Organism-specific databases

HPAiHPA049781.

Interactioni

Protein-protein interaction databases

BioGridi114522. 44 interactors.
IntActiO43252. 12 interactors.
MINTiMINT-1372331.
STRINGi9606.ENSP00000265174.

Structurei

Secondary structure

1624
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 43Combined sources7
Beta strandi44 – 49Combined sources6
Beta strandi53 – 58Combined sources6
Helixi65 – 78Combined sources14
Beta strandi83 – 87Combined sources5
Helixi88 – 91Combined sources4
Turni92 – 98Combined sources7
Helixi103 – 122Combined sources20
Beta strandi126 – 130Combined sources5
Helixi136 – 148Combined sources13
Beta strandi153 – 159Combined sources7
Helixi162 – 168Combined sources7
Helixi173 – 178Combined sources6
Beta strandi181 – 184Combined sources4
Turni186 – 188Combined sources3
Beta strandi199 – 203Combined sources5
Turni204 – 206Combined sources3
Helixi209 – 222Combined sources14
Helixi241 – 243Combined sources3
Helixi244 – 252Combined sources9
Beta strandi256 – 258Combined sources3
Helixi261 – 271Combined sources11
Turni272 – 277Combined sources6
Helixi284 – 293Combined sources10
Beta strandi294 – 296Combined sources3
Beta strandi310 – 312Combined sources3
Helixi314 – 320Combined sources7
Beta strandi324 – 330Combined sources7
Beta strandi333 – 345Combined sources13
Helixi348 – 356Combined sources9
Helixi364 – 371Combined sources8
Beta strandi374 – 383Combined sources10
Helixi394 – 396Combined sources3
Helixi400 – 409Combined sources10
Beta strandi413 – 422Combined sources10
Helixi426 – 442Combined sources17
Beta strandi445 – 454Combined sources10
Helixi465 – 477Combined sources13
Helixi483 – 485Combined sources3
Beta strandi486 – 488Combined sources3
Helixi499 – 512Combined sources14
Beta strandi516 – 520Combined sources5
Turni530 – 532Combined sources3
Beta strandi534 – 537Combined sources4
Helixi541 – 548Combined sources8
Beta strandi556 – 559Combined sources4
Beta strandi563 – 566Combined sources4
Turni567 – 570Combined sources4
Beta strandi571 – 574Combined sources4
Helixi580 – 582Combined sources3
Helixi588 – 596Combined sources9
Helixi608 – 621Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X6VX-ray1.75A/B1-624[»]
1XJQX-ray2.06A/B1-624[»]
1XNJX-ray1.98A/B1-624[»]
2OFWX-ray2.05A/B/C/D/E/F/G/H24-225[»]
2OFXX-ray1.90A/B25-227[»]
2PEYX-ray1.88A/B51-226[»]
2PEZX-ray1.40A/B51-226[»]
2QJFX-ray2.20A/B220-624[»]
ProteinModelPortaliO43252.
SMRiO43252.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43252.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – ?220Adenylyl-sulfate kinaseAdd BLAST220
Regioni?221 – 624Sulfate adenylyltransferaseAdd BLAST404

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi521 – 525PP-motif5

Sequence similaritiesi

In the N-terminal section; belongs to the APS kinase family.Curated
In the C-terminal section; belongs to the sulfate adenylyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG0635. Eukaryota.
KOG4238. Eukaryota.
COG0529. LUCA.
COG2046. LUCA.
GeneTreeiENSGT00390000009613.
HOVERGENiHBG053503.
KOiK13811.
OMAiRRPEFYY.
OrthoDBiEOG091G07ZR.
PhylomeDBiO43252.
TreeFamiTF313143.

Family and domain databases

CDDicd02027. APSK. 1 hit.
cd00517. ATPS. 1 hit.
Gene3Di3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiIPR002891. APS_kinase.
IPR025980. ATP-Sase_PUA-like_dom.
IPR027417. P-loop_NTPase.
IPR015947. PUA-like_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR024951. Sulfurylase_cat_dom.
IPR002650. Sulphate_adenylyltransferase.
[Graphical view]
PfamiPF01747. ATP-sulfurylase. 1 hit.
PF14306. PUA_2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR00339. sopT. 1 hit.

Sequencei

Sequence statusi: Complete.

O43252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIPGSLCKK VKLSNNAQNW GMQRATNVTY QAHHVSRNKR GQVVGTRGGF
60 70 80 90 100
RGCTVWLTGL SGAGKTTVSM ALEEYLVCHG IPCYTLDGDN IRQGLNKNLG
110 120 130 140 150
FSPEDREENV RRIAEVAKLF ADAGLVCITS FISPYTQDRN NARQIHEGAS
160 170 180 190 200
LPFFEVFVDA PLHVCEQRDV KGLYKKARAG EIKGFTGIDS EYEKPEAPEL
210 220 230 240 250
VLKTDSCDVN DCVQQVVELL QERDIVPVDA SYEVKELYVP ENKLHLAKTD
260 270 280 290 300
AETLPALKIN KVDMQWVQVL AEGWATPLNG FMREREYLQC LHFDCLLDGG
310 320 330 340 350
VINLSVPIVL TATHEDKERL DGCTAFALMY EGRRVAILRN PEFFEHRKEE
360 370 380 390 400
RCARQWGTTC KNHPYIKMVM EQGDWLIGGD LQVLDRVYWN DGLDQYRLTP
410 420 430 440 450
TELKQKFKDM NADAVFAFQL RNPVHNGHAL LMQDTHKQLL ERGYRRPVLL
460 470 480 490 500
LHPLGGWTKD DDVPLMWRMK QHAAVLEEGV LNPETTVVAI FPSPMMYAGP
510 520 530 540 550
TEVQWHCRAR MVAGANFYIV GRDPAGMPHP ETGKDLYEPS HGAKVLTMAP
560 570 580 590 600
GLITLEIVPF RVAAYNKKKK RMDYYDSEHH EDFEFISGTR MRKLAREGQK
610 620
PPEGFMAPKA WTVLTEYYKS LEKA
Length:624
Mass (Da):70,833
Last modified:October 10, 2002 - v2
Checksum:iA3DC9B943E68CDD6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti456G → A in CAA71413 (PubMed:9576487).Curated1
Sequence conflicti456Missing in AAC39894 (PubMed:9668121).Curated1
Sequence conflicti519 – 520IV → MC in AAD09325 (Ref. 4) Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014065270L → F.1 PublicationCorresponds to variant rs1127008dbSNPEnsembl.1
Natural variantiVAR_014064587S → L.3 PublicationsCorresponds to variant rs1127014dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10387 mRNA. Translation: CAA71413.1.
U53447 Genomic DNA. Translation: AAC39894.1.
AF033026 mRNA. Translation: AAC28429.1.
AF016496 mRNA. Translation: AAD09325.1.
AF105227 mRNA. Translation: AAF40236.1.
AF097721
, AF097710, AF097711, AF097712, AF097713, AF097714, AF097715, AF097716, AF097717, AF097718, AF097719, AF097720 Genomic DNA. Translation: AAF40235.1.
BC011392 mRNA. Translation: AAH11392.1.
BC050627 mRNA. Translation: AAH50627.1.
CCDSiCCDS3676.1.
PIRiJW0087.
RefSeqiNP_005434.4. NM_005443.4.
XP_011530702.1. XM_011532400.1.
XP_011530703.1. XM_011532401.1.
UniGeneiHs.368610.

Genome annotation databases

EnsembliENST00000265174; ENSP00000265174; ENSG00000138801.
GeneIDi9061.
KEGGihsa:9061.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10387 mRNA. Translation: CAA71413.1.
U53447 Genomic DNA. Translation: AAC39894.1.
AF033026 mRNA. Translation: AAC28429.1.
AF016496 mRNA. Translation: AAD09325.1.
AF105227 mRNA. Translation: AAF40236.1.
AF097721
, AF097710, AF097711, AF097712, AF097713, AF097714, AF097715, AF097716, AF097717, AF097718, AF097719, AF097720 Genomic DNA. Translation: AAF40235.1.
BC011392 mRNA. Translation: AAH11392.1.
BC050627 mRNA. Translation: AAH50627.1.
CCDSiCCDS3676.1.
PIRiJW0087.
RefSeqiNP_005434.4. NM_005443.4.
XP_011530702.1. XM_011532400.1.
XP_011530703.1. XM_011532401.1.
UniGeneiHs.368610.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X6VX-ray1.75A/B1-624[»]
1XJQX-ray2.06A/B1-624[»]
1XNJX-ray1.98A/B1-624[»]
2OFWX-ray2.05A/B/C/D/E/F/G/H24-225[»]
2OFXX-ray1.90A/B25-227[»]
2PEYX-ray1.88A/B51-226[»]
2PEZX-ray1.40A/B51-226[»]
2QJFX-ray2.20A/B220-624[»]
ProteinModelPortaliO43252.
SMRiO43252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114522. 44 interactors.
IntActiO43252. 12 interactors.
MINTiMINT-1372331.
STRINGi9606.ENSP00000265174.

PTM databases

iPTMnetiO43252.
PhosphoSitePlusiO43252.

Polymorphism and mutation databases

BioMutaiPAPSS1.

Proteomic databases

EPDiO43252.
PaxDbiO43252.
PeptideAtlasiO43252.
PRIDEiO43252.

Protocols and materials databases

DNASUi9061.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265174; ENSP00000265174; ENSG00000138801.
GeneIDi9061.
KEGGihsa:9061.

Organism-specific databases

CTDi9061.
DisGeNETi9061.
GeneCardsiPAPSS1.
HGNCiHGNC:8603. PAPSS1.
HPAiHPA049781.
MIMi603262. gene.
neXtProtiNX_O43252.
OpenTargetsiENSG00000138801.
PharmGKBiPA384.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0635. Eukaryota.
KOG4238. Eukaryota.
COG0529. LUCA.
COG2046. LUCA.
GeneTreeiENSGT00390000009613.
HOVERGENiHBG053503.
KOiK13811.
OMAiRRPEFYY.
OrthoDBiEOG091G07ZR.
PhylomeDBiO43252.
TreeFamiTF313143.

Enzyme and pathway databases

UniPathwayiUPA00097.
BioCyciMetaCyc:HS06566-MONOMER.
ZFISH:HS06566-MONOMER.
BRENDAi2.7.1.25. 2681.
2.7.7.4. 2681.
ReactomeiR-HSA-174362. Transport and synthesis of PAPS.
R-HSA-2408550. Metabolism of ingested H2SeO4 and H2SeO3 into H2Se.
R-HSA-6802952. Signaling by BRAF and RAF fusions.
SABIO-RKO43252.

Miscellaneous databases

ChiTaRSiPAPSS1. human.
EvolutionaryTraceiO43252.
GeneWikiiPAPSS1.
GenomeRNAii9061.
PROiO43252.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138801.
CleanExiHS_PAPSS1.
ExpressionAtlasiO43252. baseline and differential.
GenevisibleiO43252. HS.

Family and domain databases

CDDicd02027. APSK. 1 hit.
cd00517. ATPS. 1 hit.
Gene3Di3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiIPR002891. APS_kinase.
IPR025980. ATP-Sase_PUA-like_dom.
IPR027417. P-loop_NTPase.
IPR015947. PUA-like_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR024951. Sulfurylase_cat_dom.
IPR002650. Sulphate_adenylyltransferase.
[Graphical view]
PfamiPF01747. ATP-sulfurylase. 1 hit.
PF14306. PUA_2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
TIGR00339. sopT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAPS1_HUMAN
AccessioniPrimary (citable) accession number: O43252
Secondary accession number(s): O43841
, O75332, Q96FB1, Q96TF4, Q9P1P9, Q9UE98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 10, 2002
Last modified: November 30, 2016
This is version 176 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.