Reviewed, UniProtKB/Swiss-Prot O43242 (PSMD3_HUMAN)

Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 3
      Short name=26S proteasome regulatory subunit S3
Alternative name(s):
    Proteasome subunit p58

Gene names

Name:

PSMD3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Subunit structure	The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.
Sequence similarities	Belongs to the proteasome subunit S3 family. Contains 1 PCI domain.

Ontologies

Keywords
Cellular component	Proteasome
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome regulation of protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW proteasome complex Traceable author statement. Source: ProtInc
Molecular function	enzyme regulator activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
C20orf24	Q9BUV8	1	EBI-357622,EBI-1050079
PAAF1	Q9BRP4	1	EBI-357622,EBI-1056358
PSMD10	O75832	1	EBI-357622,EBI-752185
PSMD6	Q15008	1	EBI-357622,EBI-359701
PTP4A3	O75365	1	EBI-357622,EBI-1043866
WDR8	Q9P2S5	1	EBI-357622,EBI-1054904

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	26S proteasome non-ATPase regulatory subunit 3		PRO_0000173816
Regions
	Domain	358 – 462	105	PCI
	Compositional bias	26 – 32	7	Poly-Pro
Experimental info
	Sequence conflict	60	1	A → V in BAA23651. Ref.1
	Sequence conflict	157 – 158	2	TP → NT in AAH12302. Ref.3
	Sequence conflict	304	1	E → V in AAH12302. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O43242-1 [UniParc]. Last modified May 10, 2002. Version 2. Checksum: 3B3FB5593542C078	FASTA	534	60,978
	10 20 30 40 50 60 MKQEGSARRR GADKAKPPPG GGEQEPPPPP APQDVEMKEE AATGGGSTGE ADGKTAAAAA 70 80 90 100 110 120 EHSQRELDTV TLEDIKEHVK QLEKAVSGKE PRFVLRALRM LPSTSRRLNH YVLYKAVQGF 130 140 150 160 170 180 FTSNNATRDF LLPFLEEPMD TEADLQFRPR TGKAASTPLL PEVEAYLQLL VVIFMMNSKR 190 200 210 220 230 240 YKEAQKISDD LMQKISTQNR RALDLVAAKC YYYHARVYEF LDKLDVVRSF LHARLRTATL 250 260 270 280 290 300 RHDADGQATL LNLLLRNYLH YSLYDQAEKL VSKSVFPEQA NNNEWARYLY YTGRIKAIQL 310 320 330 340 350 360 EYSEARRTMT NALRKAPQHT AVGFKQTVHK LLIVVELLLG EIPDRLQFRQ PSLKRSLMPY 370 380 390 400 410 420 FLLTQAVRTG NLAKFNQVLD QFGEKFQADG TYTLIIRLRH NVIKTGVRMI SLSYSRISLA 430 440 450 460 470 480 DIAQKLQLDS PEDAEFIVAK AIRDGVIEAS INHEKGYVQS KEMIDIYSTR EPQLAFHQRI 490 500 510 520 530 SFCLDIHNMS VKAMRFPPKS YNKDLESAEE RREREQQDLE FAKEMAEDDD DSFP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast counterparts of subunits S5a and p58 (S3) of the human 26S proteasome are encoded by two multicopy suppressors of nin1-1." Kominami K., Okura N., Kawamura M., Demartino G.N., Slaughter C.A., Shimbara N., Chung C.H., Fujimuro M., Yokosawa H., Shimizu Y., Tanahashi N., Tanaka K., Toh-e A. Mol. Biol. Cell 8:171-187(1997) [PubMed: 9017604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hepatoblastoma.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye, Lung, Pancreas and Skin.
[4]	"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D67025 mRNA. Translation: BAA23651.1. BT007217 mRNA. Translation: AAP35881.1. BC000074 mRNA. Translation: AAH00074.1. BC004859 mRNA. Translation: AAH04859.1. BC012302 mRNA. Translation: AAH12302.1. BC020518 mRNA. Translation: AAH20518.1. BC025686 mRNA. Translation: AAH25686.1.
IPI	IPI00011603.
RefSeq	NP_002800.2.
UniGene	Hs.12970
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:27571N.
IntAct	O43242. 16 interactions.
PTM databases
PhosphoSite	O43242.
Proteomic databases
PeptideAtlas	O43242.
PRIDE	O43242.
Genome annotation databases
Ensembl	ENSG00000108344. Homo sapiens. [Contig view]
GeneID	5709.
KEGG	hsa:5709.
NMPDR	fig|9606.3.peg.13656.
UCSC	uc002htn.1. human.
Organism-specific databases
GeneCards	GC17P035390.
H-InvDB	HIX0013776.
HGNC	HGNC:9560. PSMD3.
HPA	CAB017038.
PharmGKB	PA33906.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	O43242.
OMA	O43242. LMVIFLM.
Enzyme and pathway databases
Reactome	REACT_11045. Signaling by Wnt. REACT_13. Metabolism of amino acids. REACT_13635. Regulation of activated PAK-2p34 by proteasome mediated degradation. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.
Gene expression databases
ArrayExpress	O43242.
Bgee	O43242.
CleanEx	HS_PSMD3.
GermOnline	ENSG00000108344. Homo sapiens.
Family and domain databases
InterPro	IPR013586. 26S_Psome_reg_C. IPR013143. PAM. IPR000717. PCI. [Graphical view]
Pfam	PF01399. PCI. 1 hit. PF08375. Rpn3_C. 1 hit. [Graphical view]
SMART	SM00753. PAM. 1 hit. SM00088. PINT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	22182.

Entry information

Entry name

PSMD3_HUMAN

Accession

Primary (citable) accession number: O43242
Secondary accession number(s): Q96EI2, Q9BQA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 10, 2002
Last modified:	July 7, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)