O43240 (KLK10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kallikrein-10
EC=3.4.21.-

Alternative name(s):
Normal epithelial cell-specific 1
Protease serine-like 1

Gene names

Name:	KLK10
Synonyms:	NES1, PRSSL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a tumor-suppressor role for NES1 in breast and prostate cancer.
Subcellular location	Secreted Probable.
Tissue specificity	Expressed in breast, ovary and prostate.
Developmental stage	Down-regulated during breast cancer progression.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Disease	Tumor suppressor
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Traceable author statement Ref.1. Source: ProtInc
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type peptidase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 276	246	Kallikrein-10		PRO_0000027953
Regions
	Domain	47 – 274	228	Peptidase S1
Sites
	Active site	86	1	Charge relay system By similarity
	Active site	137	1	Charge relay system By similarity
	Active site	229	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Disulfide bond	52 ↔ 162		By similarity
	Disulfide bond	71 ↔ 87		By similarity
	Disulfide bond	169 ↔ 235		By similarity
	Disulfide bond	201 ↔ 215		By similarity
	Disulfide bond	225 ↔ 250		By similarity
	Disulfide bond	? ↔ 263		By similarity
Natural variations
	Natural variant	50	1	S → A. Ref.1 Ref.2 Ref.6 Corresponds to variant rs3745535 [ dbSNP | Ensembl ].		VAR_027979
	Natural variant	149	1	L → P. Ref.1 Ref.2 Ref.6 Corresponds to variant rs2075690 [ dbSNP | Ensembl ].		VAR_027980

Sequences

	Sequence		Length	Mass (Da)
	O43240 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: 4CFB32E0AF686E96	FASTA	276	30,170
	10 20 30 40 50 60 MRAPHLHLSA ASGARALAKL LPLLMAQLWA AEAALLPQND TRLDPEAYGS PCARGSQPWQ 70 80 90 100 110 120 VSLFNGLSFH CAGVLVDQSW VLTAAHCGNK PLWARVGDDH LLLLQGEQLR RTTRSVVHPK 130 140 150 160 170 180 YHQGSGPILP RRTDEHDLML LKLARPVVLG PRVRALQLPY RCAQPGDQCQ VAGWGTTAAR 190 200 210 220 230 240 RVKYNKGLTC SSITILSPKE CEVFYPGVVT NNMICAGLDR GQDPCQSDSG GPLVCDETLQ 250 260 270 GILSWGVYPC GSAQHPAVYT QICKYMSWIN KVIRSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel serine protease-like gene, the expression of which is down-regulated during breast cancer progression." Liu X.-L., Wazer D.E., Watanabe K., Band V. Cancer Res. 56:3371-3379(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ALA-50 AND PRO-149. Tissue: Epithelium.
[2]	"Structural characterization and mapping of the normal epithelial cell-specific 1 gene." Luo L.-Y., Herbrick J.A., Scherer S.W., Beatty B., Squire J., Diamandis E.P. Biochem. Biophys. Res. Commun. 247:580-586(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-50 AND PRO-149.
[3]	"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region." Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K. Gene 257:119-130(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Identification of new splice variants and differential expression of the human kallikrein 10 gene, a candidate cancer biomarker." Yousef G.M., White N.M.A., Michael I.P., Cho J.C.-K., Robb J.D., Kurlender L., Khan S., Diamandis E.P. Tumor Biol. 26:227-235(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ALA-50 AND PRO-149. Tissue: Uterus.
[7]	"The role for NES1 serine protease as a novel tumor suppressor." Goyal J., Smith K.M., Cowan J.M., Wazer D.E., Lee S.W., Band V. Cancer Res. 58:4782-4786(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF024605 mRNA. Translation: AAB81602.1. AF055481 Genomic DNA. Translation: AAC14266.1. AF243527 Genomic DNA. Translation: AAG33363.1. AY561635 mRNA. Translation: AAS66976.1. AC011473 Genomic DNA. Translation: AAG23256.1. BC002710 mRNA. Translation: AAH02710.1.
IPI	IPI00480121.
RefSeq	NP_001070968.1. NM_001077500.1. NP_002767.2. NM_002776.4. NP_665895.1. NM_145888.2.
UniGene	Hs.275464.
3D structure databases
ProteinModelPortal	O43240.
SMR	O43240. Positions 49-276.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43240. 2 interactions.
MINT	MINT-1406798.
STRING	9606.ENSP00000311746.
Protein family/group databases
MEROPS	S01.246.
Proteomic databases
PaxDb	O43240.
PRIDE	O43240.
Protocols and materials databases
DNASU	5655.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000309958; ENSP00000311746; ENSG00000129451. ENST00000358789; ENSP00000351640; ENSG00000129451. ENST00000391805; ENSP00000375681; ENSG00000129451.
GeneID	5655.
KEGG	hsa:5655.
UCSC	uc002puy.3. human.
Organism-specific databases
CTD	5655.
GeneCards	GC19M051516.
H-InvDB	HIX0015374.
HGNC	HGNC:6358. KLK10.
HPA	CAB017133. HPA017195.
MIM	602673. gene.
neXtProt	NX_O43240.
PharmGKB	PA30147.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	O43240.
KO	K09619.
OMA	NKPLWAR.
OrthoDB	EOG43TZW3.
Gene expression databases
Bgee	O43240.
CleanEx	HS_KLK10. HS_PRSSL1.
Genevestigator	O43240.
GermOnline	ENSG00000129451. Homo sapiens.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O43240.
ChEMBL	CHEMBL4180.
ChiTaRS	KLK10. human.
GenomeRNAi	5655.
NextBio	21978.
SOURCE	Search...

Entry information

Entry name

KLK10_HUMAN

Accession

Primary (citable) accession number: O43240
Secondary accession number(s): A6NC12 , Q53YL3, Q99920, Q9GZW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.