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Protein

Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase

Gene

ST8SIA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sialic acid from a CMP-linked sialic acid donor onto the terminal sialic acid of an acceptor through alpha-2,8-linkages. Is active with alpha-2,3-linked, alpha-2,6-linked and alpha-2,8-linked sialic acid of N-linked oligosaccharides of glycoproteins and glycolipids. Displays preference for substrates with alpha-2,3-linked terminal sialic acid. It can form polysialic acid in vitro directly on alpha-2,3-, alpha-2,6-, or alpha-2,8-linked sialic acid.3 Publications

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.2 Publications
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei190Substrate1 Publication1
Active sitei354Proton donor/acceptor1 Publication1

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: BHF-UCL
  • sialic acid binding Source: BHF-UCL
  • sialyltransferase activity Source: UniProtKB

GO - Biological processi

  • ganglioside biosynthetic process Source: BHF-UCL
  • glycoprotein metabolic process Source: BHF-UCL
  • glycosphingolipid biosynthetic process Source: ProtInc
  • N-glycan processing Source: UniProtKB
  • oligosaccharide metabolic process Source: BHF-UCL
  • protein glycosylation Source: BHF-UCL
  • protein sialylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciZFISH:ENSG00000177511-MONOMER.
ReactomeiR-HSA-4085001. Sialic acid metabolism.
R-HSA-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase (EC:2.4.99.-1 Publication)
Alternative name(s):
Alpha-2,8-sialyltransferase 8C
Alpha-2,8-sialyltransferase III
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Sialyltransferase 8C
Short name:
SIAT8-C
Sialyltransferase St8Sia III1 Publication
Short name:
ST8SiaIII1 Publication
Gene namesi
Name:ST8SIA3
Synonyms:SIAT8C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:14269. ST8SIA3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 33Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST24
Topological domaini34 – 380LumenalSequence analysisAdd BLAST347

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi354H → A: Abolishes enzyme activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000177511.
PharmGKBiPA37864.

Polymorphism and mutation databases

BioMutaiST8SIA3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492891 – 380Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferaseAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi93N-linked (GlcNAc...)Sequence analysis1 Publication1
Glycosylationi113N-linked (GlcNAc...)1 Publication1
Glycosylationi160N-linked (GlcNAc...); atypical1 Publication1
Disulfide bondi162 ↔ 313Combined sources1 Publication
Disulfide bondi176 ↔ 379Combined sources1 Publication
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO43173.
PeptideAtlasiO43173.
PRIDEiO43173.

PTM databases

iPTMnetiO43173.
PhosphoSitePlusiO43173.

Expressioni

Tissue specificityi

Expressed in fetal and adult brain and fetal liver.1 Publication

Gene expression databases

BgeeiENSG00000177511.
CleanExiHS_ST8SIA3.
ExpressionAtlasiO43173. baseline and differential.
GenevisibleiO43173. HS.

Organism-specific databases

HPAiHPA063398.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi119243. 11 interactors.
DIPiDIP-61706N.
STRINGi9606.ENSP00000320431.

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi94 – 107Combined sources14
Helixi110 – 113Combined sources4
Helixi118 – 120Combined sources3
Helixi142 – 146Combined sources5
Turni153 – 156Combined sources4
Beta strandi159 – 165Combined sources7
Helixi169 – 171Combined sources3
Helixi177 – 181Combined sources5
Beta strandi183 – 189Combined sources7
Helixi195 – 197Combined sources3
Helixi198 – 201Combined sources4
Beta strandi206 – 209Combined sources4
Helixi214 – 218Combined sources5
Helixi220 – 222Combined sources3
Helixi225 – 236Combined sources12
Turni237 – 240Combined sources4
Beta strandi242 – 244Combined sources3
Helixi251 – 253Combined sources3
Helixi254 – 266Combined sources13
Turni267 – 269Combined sources3
Beta strandi274 – 276Combined sources3
Helixi282 – 291Combined sources10
Helixi301 – 312Combined sources12
Beta strandi313 – 320Combined sources8
Beta strandi324 – 326Combined sources3
Turni328 – 330Combined sources3
Beta strandi351 – 353Combined sources3
Helixi356 – 368Combined sources13
Beta strandi371 – 374Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5BO6X-ray2.07A/B81-380[»]
5BO7X-ray1.85A/B81-380[»]
5BO8X-ray2.70A/B61-380[»]
5BO9X-ray2.30A/B81-380[»]
5CXYX-ray2.15A/B81-380[»]
ProteinModelPortaliO43173.
SMRiO43173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 213Substrate binding1 Publication3
Regioni300 – 302Substrate binding1 Publication3
Regioni336 – 337Substrate binding1 Publication2

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOVERGENiHBG056466.
InParanoidiO43173.
KOiK06613.
OMAiHELQEKP.
OrthoDBiEOG091G0AL4.
PhylomeDBiO43173.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

O43173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNCKMARVA SVLGLVMLSV ALLILSLISY VSLKKENIFT TPKYASPGAP
60 70 80 90 100
RMYMFHAGFR SQFALKFLDP SFVPITNSLT QELQEKPSKW KFNRTAFLHQ
110 120 130 140 150
RQEILQHVDV IKNFSLTKNS VRIGQLMHYD YSSHKYVFSI SNNFRSLLPD
160 170 180 190 200
VSPIMNKHYN ICAVVGNSGI LTGSQCGQEI DKSDFVFRCN FAPTEAFQRD
210 220 230 240 250
VGRKTNLTTF NPSILEKYYN NLLTIQDRNN FFLSLKKLDG AILWIPAFFF
260 270 280 290 300
HTSATVTRTL VDFFVEHRGQ LKVQLAWPGN IMQHVNRYWK NKHLSPKRLS
310 320 330 340 350
TGILMYTLAS AICEEIHLYG FWPFGFDPNT REDLPYHYYD KKGTKFTTKW
360 370 380
QESHQLPAEF QLLYRMHGEG LTKLTLSHCA
Length:380
Mass (Da):43,970
Last modified:October 17, 2006 - v3
Checksum:i70D782A2CAD2666A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173 – 174GS → FI in AAB87642 (PubMed:9826427).Curated2
Sequence conflicti178Q → R in AAB87642 (PubMed:9826427).Curated1
Sequence conflicti194T → S in AAB87642 (PubMed:9826427).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02024991K → T.Corresponds to variant rs3745060dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004668 mRNA. Translation: AAB87642.1.
AF003092 mRNA. Translation: AAC15901.2.
AK289517 mRNA. Translation: BAF82206.1.
AK313047 mRNA. Translation: BAG35879.1.
BC074909 mRNA. Translation: AAH74909.1.
CCDSiCCDS32834.1.
RefSeqiNP_056963.2. NM_015879.2.
UniGeneiHs.23172.

Genome annotation databases

EnsembliENST00000324000; ENSP00000320431; ENSG00000177511.
GeneIDi51046.
KEGGihsa:51046.
UCSCiuc002lgn.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia III

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004668 mRNA. Translation: AAB87642.1.
AF003092 mRNA. Translation: AAC15901.2.
AK289517 mRNA. Translation: BAF82206.1.
AK313047 mRNA. Translation: BAG35879.1.
BC074909 mRNA. Translation: AAH74909.1.
CCDSiCCDS32834.1.
RefSeqiNP_056963.2. NM_015879.2.
UniGeneiHs.23172.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5BO6X-ray2.07A/B81-380[»]
5BO7X-ray1.85A/B81-380[»]
5BO8X-ray2.70A/B61-380[»]
5BO9X-ray2.30A/B81-380[»]
5CXYX-ray2.15A/B81-380[»]
ProteinModelPortaliO43173.
SMRiO43173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119243. 11 interactors.
DIPiDIP-61706N.
STRINGi9606.ENSP00000320431.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiO43173.
PhosphoSitePlusiO43173.

Polymorphism and mutation databases

BioMutaiST8SIA3.

Proteomic databases

PaxDbiO43173.
PeptideAtlasiO43173.
PRIDEiO43173.

Protocols and materials databases

DNASUi51046.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324000; ENSP00000320431; ENSG00000177511.
GeneIDi51046.
KEGGihsa:51046.
UCSCiuc002lgn.4. human.

Organism-specific databases

CTDi51046.
GeneCardsiST8SIA3.
HGNCiHGNC:14269. ST8SIA3.
HPAiHPA063398.
MIMi609478. gene.
neXtProtiNX_O43173.
OpenTargetsiENSG00000177511.
PharmGKBiPA37864.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOVERGENiHBG056466.
InParanoidiO43173.
KOiK06613.
OMAiHELQEKP.
OrthoDBiEOG091G0AL4.
PhylomeDBiO43173.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciZFISH:ENSG00000177511-MONOMER.
ReactomeiR-HSA-4085001. Sialic acid metabolism.
R-HSA-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiST8SIA3. human.
GenomeRNAii51046.
PROiO43173.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000177511.
CleanExiHS_ST8SIA3.
ExpressionAtlasiO43173. baseline and differential.
GenevisibleiO43173. HS.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIA8C_HUMAN
AccessioniPrimary (citable) accession number: O43173
Secondary accession number(s): A8K0F2, Q6B085, Q9NS41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.