O43169 (CYB5B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b5 type B

Alternative name(s):
Cytochrome b5 outer mitochondrial membrane isoform

Gene names

Name:	CYB5B
Synonyms:	CYB5M, OMB5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.
Subunit structure	Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2 By similarity.
Subcellular location	Mitochondrion outer membrane By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme binding Inferred from direct assay PubMed 20861021. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Propeptide

1 – 11

11

By similarity

PRO_0000006471

Chain

12 – 146

135

Cytochrome b5 type B

PRO_0000006472

Regions

Transmembrane

119 – 136

18

Helical; Potential

Domain

20 – 96

77

Cytochrome b5 heme-binding

Sites

Metal binding

55

1

Iron (heme axial ligand) By similarity

Metal binding

79

1

Iron (heme axial ligand) By similarity

Amino acid modifications

Modified residue

30

1

N6-acetyllysine Ref.6

Modified residue

33

1

Phosphoserine By similarity

Experimental info

Sequence conflict

111

1

K → Q in BAA23735. Ref.1

Secondary structure

1

.

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146

Helix Strand Turn

Details...

Helix

25 – 28

4

Beta strand

36 – 41

6

Beta strand

44 – 47

4

Helix

49 – 51

3

Turn

52 – 54

3

Helix

60 – 63

4

Turn

64 – 67

4

Helix

71 – 76

6

Helix

81 – 87

7

Helix

88 – 90

3

Beta strand

91 – 95

5

Helix

97 – 99

3

Sequences

	Sequence		Length	Mass (Da)
	O43169 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: 2FF7DBB62859AE19	FASTA	146	16,332
	10 20 30 40 50 60 MATAEASGSD GKGQEVETSV TYYRLEEVAK RNSLKELWLV IHGRVYDVTR FLNEHPGGEE 70 80 90 100 110 120 VLLEQAGVDA SESFEDVGHS SDAREMLKQY YIGDIHPSDL KPESGSKDPS KNDTCKSCWA 130 140 YWILPIIGAV LLGFLYRYYT SESKSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cytochrome b5 and aquaporins share the last transmembrane amino acids sequence." Ishibashi K. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[3]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph and Pancreas.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-30, MASS SPECTROMETRY.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB009282 mRNA. Translation: BAA23735.1.
AK291576 mRNA. Translation: BAF84265.1.
AC026464 Genomic DNA. No translation available.
BC004373 mRNA. Translation: AAH04373.1.
BC014431 mRNA. Translation: AAH14431.2.

IPI

IPI00303954.

RefSeq

NP_085056.2. NM_030579.2.

UniGene

Hs.461131.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3NER	X-ray	1.45	A/B	12-103	[»]

ProteinModelPortal

O43169.

ModBase

Search...

Protein-protein interaction databases

IntAct

O43169. 1 interaction.

STRING

9606.ENSP00000308430.

PTM databases

PhosphoSite

O43169.

Proteomic databases

PaxDb

O43169.

PRIDE

O43169.

Protocols and materials databases

DNASU

80777.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000512062; ENSP00000423679; ENSG00000103018.

GeneID

80777.

KEGG

hsa:80777.

UCSC

uc002exf.3. human.

Organism-specific databases

CTD

80777.

GeneCards

GC16P069458.

HGNC

HGNC:24374. CYB5B.

HPA

HPA007893.

MIM

611964. gene.

neXtProt

NX_O43169.

PharmGKB

PA143485445.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5274.

HOGENOM

HOG000039853.

HOVERGEN

HBG002653.

InParanoid

O43169.

Gene expression databases

ArrayExpress

O43169.

Bgee

O43169.

CleanEx

HS_CYB5B.

Genevestigator

O43169.

GermOnline

ENSG00000103018. Homo sapiens.

Family and domain databases

Gene3D

3.10.120.10. 1 hit.

InterPro

IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]

Pfam

PF00173. Cyt-b5. 1 hit.
[Graphical view]

PRINTS

PR00363. CYTOCHROMEB5.

SUPFAM

SSF55856. Cyt_B5. 1 hit.

PROSITE

PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CYB5B. human.

GenomeRNAi

80777.

NextBio

71179.

PMAP-CutDB

O43169.

SOURCE

Search...

Entry information

Entry name

CYB5B_HUMAN

Accession

Primary (citable) accession number: O43169
Secondary accession number(s): A8K6B1, Q96CC3, Q9BT35

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.