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Protein

Cytochrome b5 type B

Gene

CYB5B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi79Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

  • heme binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103018-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5 type B
Alternative name(s):
Cytochrome b5 outer mitochondrial membrane isoform
Gene namesi
Name:CYB5B
Synonyms:CYB5M, OMB5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:24374. CYB5B.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei119 – 136HelicalSequence analysisAdd BLAST18

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • mitochondrial outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi80777.
OpenTargetsiENSG00000103018.
PharmGKBiPA143485445.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
PropeptideiPRO_00000064712 – 11By similarity10
ChainiPRO_000000647212 – 146Cytochrome b5 type BAdd BLAST135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei19PhosphoserineCombined sources1
Modified residuei30N6-acetyllysineCombined sources1
Modified residuei33PhosphoserineBy similarity1
Modified residuei35N6-methyllysineCombined sources1
Modified residuei80PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO43169.
MaxQBiO43169.
PaxDbiO43169.
PeptideAtlasiO43169.
PRIDEiO43169.
TopDownProteomicsiO43169.

PTM databases

iPTMnetiO43169.
PhosphoSitePlusiO43169.
SwissPalmiO43169.

Miscellaneous databases

PMAP-CutDBO43169.

Expressioni

Gene expression databases

BgeeiENSG00000103018.
CleanExiHS_CYB5B.
ExpressionAtlasiO43169. baseline and differential.
GenevisibleiO43169. HS.

Organism-specific databases

HPAiHPA007893.

Interactioni

Subunit structurei

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Q99IB85EBI-1058710,EBI-6928570From a different organism.

Protein-protein interaction databases

BioGridi123309. 34 interactors.
IntActiO43169. 6 interactors.
MINTiMINT-5000089.
STRINGi9606.ENSP00000308430.

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 28Combined sources4
Beta strandi36 – 41Combined sources6
Beta strandi44 – 47Combined sources4
Helixi49 – 51Combined sources3
Turni52 – 54Combined sources3
Helixi60 – 63Combined sources4
Turni64 – 67Combined sources4
Helixi71 – 76Combined sources6
Helixi81 – 87Combined sources7
Helixi88 – 90Combined sources3
Beta strandi91 – 95Combined sources5
Helixi97 – 99Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NERX-ray1.45A/B12-103[»]
ProteinModelPortaliO43169.
SMRiO43169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 96Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiO43169.
PhylomeDBiO43169.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
3.10.120.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43169-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAEASGSD GKGQEVETSV TYYRLEEVAK RNSLKELWLV IHGRVYDVTR
60 70 80 90 100
FLNEHPGGEE VLLEQAGVDA SESFEDVGHS SDAREMLKQY YIGDIHPSDL
110 120 130 140
KPESGSKDPS KNDTCKSCWA YWILPIIGAV LLGFLYRYYT SESKSS
Length:146
Mass (Da):16,332
Last modified:June 7, 2005 - v2
Checksum:i2FF7DBB62859AE19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111K → Q in BAA23735 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009282 mRNA. Translation: BAA23735.1.
AK291576 mRNA. Translation: BAF84265.1.
AC026464 Genomic DNA. No translation available.
BC004373 mRNA. Translation: AAH04373.1.
BC014431 mRNA. Translation: AAH14431.2.
RefSeqiNP_085056.2. NM_030579.2.
UniGeneiHs.461131.

Genome annotation databases

EnsembliENST00000512062; ENSP00000423679; ENSG00000103018.
GeneIDi80777.
KEGGihsa:80777.
UCSCiuc059wli.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009282 mRNA. Translation: BAA23735.1.
AK291576 mRNA. Translation: BAF84265.1.
AC026464 Genomic DNA. No translation available.
BC004373 mRNA. Translation: AAH04373.1.
BC014431 mRNA. Translation: AAH14431.2.
RefSeqiNP_085056.2. NM_030579.2.
UniGeneiHs.461131.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NERX-ray1.45A/B12-103[»]
ProteinModelPortaliO43169.
SMRiO43169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123309. 34 interactors.
IntActiO43169. 6 interactors.
MINTiMINT-5000089.
STRINGi9606.ENSP00000308430.

PTM databases

iPTMnetiO43169.
PhosphoSitePlusiO43169.
SwissPalmiO43169.

Proteomic databases

EPDiO43169.
MaxQBiO43169.
PaxDbiO43169.
PeptideAtlasiO43169.
PRIDEiO43169.
TopDownProteomicsiO43169.

Protocols and materials databases

DNASUi80777.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000512062; ENSP00000423679; ENSG00000103018.
GeneIDi80777.
KEGGihsa:80777.
UCSCiuc059wli.1. human.

Organism-specific databases

CTDi80777.
DisGeNETi80777.
GeneCardsiCYB5B.
HGNCiHGNC:24374. CYB5B.
HPAiHPA007893.
MIMi611964. gene.
neXtProtiNX_O43169.
OpenTargetsiENSG00000103018.
PharmGKBiPA143485445.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiO43169.
PhylomeDBiO43169.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103018-MONOMER.

Miscellaneous databases

ChiTaRSiCYB5B. human.
GenomeRNAii80777.
PMAP-CutDBO43169.
PROiO43169.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103018.
CleanExiHS_CYB5B.
ExpressionAtlasiO43169. baseline and differential.
GenevisibleiO43169. HS.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
3.10.120.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB5B_HUMAN
AccessioniPrimary (citable) accession number: O43169
Secondary accession number(s): A8K6B1, Q96CC3, Q9BT35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 7, 2005
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.