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Protein

E3 ubiquitin-protein ligase Praja-2

Gene

PJA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri634 – 67542RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • protein kinase A catalytic subunit binding Source: UniProtKB
  • protein kinase A regulatory subunit binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • hippo signaling Source: InterPro
  • long-term memory Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • regulation of protein kinase A signaling Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Praja-2 (EC:6.3.2.-)
Short name:
Praja2
Alternative name(s):
RING finger protein 131
Gene namesi
Name:PJA2
Synonyms:KIAA0438, RNF131
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:17481. PJA2.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • intermediate filament cytoskeleton Source: HPA
  • plasma membrane Source: UniProtKB
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134873520.

Polymorphism and mutation databases

BioMutaiPJA2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 708707E3 ubiquitin-protein ligase Praja-2PRO_0000278230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei196 – 1961PhosphoserineCombined sources
Modified residuei246 – 2461PhosphothreonineCombined sources
Modified residuei253 – 2531PhosphoserineCombined sources
Modified residuei309 – 3091PhosphoserineCombined sources
Modified residuei323 – 3231PhosphoserineCombined sources
Modified residuei342 – 3421Phosphoserine; by PKA1 Publication
Modified residuei389 – 3891Phosphothreonine; by PKA1 Publication
Modified residuei432 – 4321PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO43164.
MaxQBiO43164.
PaxDbiO43164.
PeptideAtlasiO43164.
PRIDEiO43164.

PTM databases

iPTMnetiO43164.
PhosphoSiteiO43164.

Expressioni

Gene expression databases

BgeeiENSG00000198961.
CleanExiHS_PJA2.
GenevisibleiO43164. HS.

Organism-specific databases

HPAiHPA040347.
HPA057636.

Interactioni

Subunit structurei

Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. The phosphorylated form interacts with PRKAR1A, PRKAR2A and PRKAR2B. Binds the catalytic subunits of cAMP-dependent protein kinase.2 Publications

GO - Molecular functioni

  • protein kinase A catalytic subunit binding Source: UniProtKB
  • protein kinase A regulatory subunit binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115200. 31 interactions.
DIPiDIP-47040N.
IntActiO43164. 25 interactions.
STRINGi9606.ENSP00000354775.

Structurei

3D structure databases

ProteinModelPortaliO43164.
SMRiO43164. Positions 603-680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni531 – 708178Interaction with PRKAR1A, PRKAR2A and PRKAR2BAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri634 – 67542RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0800. Eukaryota.
ENOG41121N2. LUCA.
GeneTreeiENSGT00530000062967.
HOGENOMiHOG000230900.
HOVERGENiHBG003815.
InParanoidiO43164.
KOiK10634.
OMAiAQPEAFM.
OrthoDBiEOG091G0306.
PhylomeDBiO43164.
TreeFamiTF330711.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR030639. Praja-2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR15710:SF5. PTHR15710:SF5. 1 hit.
PfamiPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43164-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQYTEKEPA AMDQESGKAV WPKPAGGYQT ITGRRYGRRH AYVSFKPCMT
60 70 80 90 100
RHERSLGRAG DDYEVLELDD VPKENSSGSS PLDQVDSSLP SEPIFEKSET
110 120 130 140 150
EIPTCGSALN QTTESSQSFV AVHHSEEGRD TLGSSTNLHN HSEGEYIPGA
160 170 180 190 200
CSASSVQNGI ALVHTDSYDP DGKHGEDNDH LQLSAEVVEG SRYQESLGNT
210 220 230 240 250
VFELENREAE AYTGLSPPVP SFNCEVRDEF EELDSVPLVK SSAGDTEFVH
260 270 280 290 300
QNSQEIQRSS QDEMVSTKQQ NNTSQERQTE HSPEDAACGP GHICSEQNTN
310 320 330 340 350
DREKNHGSSP EQVVRPKVRK LISSSQVDQE TGFNRHEAKQ RSVQRWREAL
360 370 380 390 400
EVEESGSDDL LIKCEEYDGE HDCMFLDPPY SRVITQRETE NNQMTSESGA
410 420 430 440 450
TAGRQEVDNT FWNGCGDYYQ LYDKDEDSSE CSDGEWSASL PHRFSGTEKD
460 470 480 490 500
QSSSDESWET LPGKDENEPE LQSDSSGPEE ENQELSLQEG EQTSLEEGEI
510 520 530 540 550
PWLQYNEVNE SSSDEGNEPA NEFAQPAFML DGNNNLEDDS SVSEDLDVDW
560 570 580 590 600
SLFDGFADGL GVAEAISYVD PQFLTYMALE ERLAQAMETA LAHLESLAVD
610 620 630 640 650
VEVANPPASK ESIDGLPETL VLEDHTAIGQ EQCCPICCSE YIKDDIATEL
660 670 680 690 700
PCHHFFHKPC VSIWLQKSGT CPVCRRHFPP AVIEASAAPS SEPDPDAPPS

NDSIAEAP
Length:708
Mass (Da):78,214
Last modified:November 24, 2009 - v4
Checksum:i719B4A367C411735
GO
Isoform 2 (identifier: O43164-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     544-563: Missing.

Note: No experimental confirmation available.
Show »
Length:688
Mass (Da):76,091
Checksum:i34A652ACAF3A02FE
GO

Sequence cautioni

The sequence BAA23710 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti176 – 1761E → G.
Corresponds to variant rs35224970 [ dbSNP | Ensembl ].
VAR_057215
Natural varianti297 – 2971Q → R.2 Publications
Corresponds to variant rs1045706 [ dbSNP | Ensembl ].
VAR_030698
Natural varianti705 – 7051A → T.
Corresponds to variant rs246105 [ dbSNP | Ensembl ].
VAR_030699

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei544 – 56320Missing in isoform 2. 1 PublicationVSP_023198Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007898 mRNA. Translation: BAA23710.2. Different initiation.
AK291759 mRNA. Translation: BAF84448.1.
AC008467 Genomic DNA. No translation available.
AC010625 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW49050.1.
CH471086 Genomic DNA. Translation: EAW49051.1.
BC030826 mRNA. Translation: AAH30826.1.
CR749579 mRNA. Translation: CAH18371.1.
CCDSiCCDS4099.1. [O43164-1]
PIRiT00064.
RefSeqiNP_055634.3. NM_014819.4. [O43164-1]
UniGeneiHs.483036.

Genome annotation databases

EnsembliENST00000361189; ENSP00000354775; ENSG00000198961. [O43164-1]
ENST00000361557; ENSP00000355284; ENSG00000198961. [O43164-1]
GeneIDi9867.
KEGGihsa:9867.
UCSCiuc003kos.5. human. [O43164-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007898 mRNA. Translation: BAA23710.2. Different initiation.
AK291759 mRNA. Translation: BAF84448.1.
AC008467 Genomic DNA. No translation available.
AC010625 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW49050.1.
CH471086 Genomic DNA. Translation: EAW49051.1.
BC030826 mRNA. Translation: AAH30826.1.
CR749579 mRNA. Translation: CAH18371.1.
CCDSiCCDS4099.1. [O43164-1]
PIRiT00064.
RefSeqiNP_055634.3. NM_014819.4. [O43164-1]
UniGeneiHs.483036.

3D structure databases

ProteinModelPortaliO43164.
SMRiO43164. Positions 603-680.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115200. 31 interactions.
DIPiDIP-47040N.
IntActiO43164. 25 interactions.
STRINGi9606.ENSP00000354775.

PTM databases

iPTMnetiO43164.
PhosphoSiteiO43164.

Polymorphism and mutation databases

BioMutaiPJA2.

Proteomic databases

EPDiO43164.
MaxQBiO43164.
PaxDbiO43164.
PeptideAtlasiO43164.
PRIDEiO43164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361189; ENSP00000354775; ENSG00000198961. [O43164-1]
ENST00000361557; ENSP00000355284; ENSG00000198961. [O43164-1]
GeneIDi9867.
KEGGihsa:9867.
UCSCiuc003kos.5. human. [O43164-1]

Organism-specific databases

CTDi9867.
GeneCardsiPJA2.
H-InvDBHIX0005075.
HGNCiHGNC:17481. PJA2.
HPAiHPA040347.
HPA057636.
neXtProtiNX_O43164.
PharmGKBiPA134873520.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0800. Eukaryota.
ENOG41121N2. LUCA.
GeneTreeiENSGT00530000062967.
HOGENOMiHOG000230900.
HOVERGENiHBG003815.
InParanoidiO43164.
KOiK10634.
OMAiAQPEAFM.
OrthoDBiEOG091G0306.
PhylomeDBiO43164.
TreeFamiTF330711.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiPJA2. human.
GeneWikiiPJA2.
GenomeRNAii9867.
PROiO43164.

Gene expression databases

BgeeiENSG00000198961.
CleanExiHS_PJA2.
GenevisibleiO43164. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR030639. Praja-2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR15710:SF5. PTHR15710:SF5. 1 hit.
PfamiPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPJA2_HUMAN
AccessioniPrimary (citable) accession number: O43164
Secondary accession number(s): A8K6U4
, D3DSZ5, Q68D49, Q8N1G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: November 24, 2009
Last modified: September 7, 2016
This is version 137 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.