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O43159

- RRP8_HUMAN

UniProt

O43159 - RRP8_HUMAN

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Protein

Ribosomal RNA-processing protein 8

Gene

RRP8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei281 – 2811S-adenosyl-L-methionine
Binding sitei316 – 3161S-adenosyl-L-methionine; via carbonyl oxygen
Binding sitei334 – 3341S-adenosyl-L-methionine
Binding sitei346 – 3461S-adenosyl-L-methionine
Binding sitei347 – 3471S-adenosyl-L-methionine
Binding sitei363 – 3631S-adenosyl-L-methionine; via carbonyl oxygen

GO - Molecular functioni

  1. methylated histone binding Source: UniProtKB
  2. poly(A) RNA binding Source: UniProtKB
  3. S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to glucose starvation Source: UniProtKB
  2. chromatin modification Source: UniProtKB-KW
  3. chromatin silencing at rDNA Source: UniProtKB
  4. intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
  5. positive regulation of cell cycle arrest Source: UniProtKB
  6. regulation of transcription by glucose Source: UniProtKB
  7. rRNA processing Source: UniProtKB-KW
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Repressor, Transferase

Keywords - Biological processi

rRNA processing, Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiREACT_200827. SIRT1 negatively regulates rRNA Expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA-processing protein 8 (EC:2.1.1.-)
Alternative name(s):
Cerebral protein 1
Nucleomethylin
Gene namesi
Name:RRP8
Synonyms:KIAA0409, NML
ORF Names:hucep-1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:29030. RRP8.

Subcellular locationi

Nucleusnucleolus 3 Publications
Note: Localizes at rDNA locus.

GO - Cellular componenti

  1. chromatin silencing complex Source: UniProtKB
  2. cytoplasm Source: HPA
  3. nucleolus Source: UniProtKB
  4. nucleus Source: HPA
  5. plasma membrane Source: HPA
  6. rDNA heterochromatin Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164725550.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Ribosomal RNA-processing protein 8PRO_0000084090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621Phosphoserine2 Publications
Modified residuei64 – 641Phosphoserine2 Publications
Modified residuei171 – 1711Phosphoserine1 Publication
Modified residuei223 – 2231Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO43159.
PaxDbiO43159.
PeptideAtlasiO43159.
PRIDEiO43159.

2D gel databases

SWISS-2DPAGEO43159.

PTM databases

PhosphoSiteiO43159.

Expressioni

Gene expression databases

BgeeiO43159.
CleanExiHS_KIAA0409.
ExpressionAtlasiO43159. baseline and differential.
GenevestigatoriO43159.

Organism-specific databases

HPAiHPA038487.

Interactioni

Subunit structurei

Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SIRT1Q96EB63EBI-2008793,EBI-1802965
SUV39H1O434633EBI-2008793,EBI-349968

Protein-protein interaction databases

BioGridi116954. 12 interactions.
DIPiDIP-46686N.
IntActiO43159. 4 interactions.
MINTiMINT-4526401.
STRINGi9606.ENSP00000254605.

Structurei

Secondary structure

1
456
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi244 – 26017Combined sources
Helixi263 – 27210Combined sources
Helixi274 – 28815Combined sources
Beta strandi291 – 2933Combined sources
Helixi295 – 30410Combined sources
Beta strandi312 – 3165Combined sources
Helixi321 – 3255Combined sources
Beta strandi330 – 3367Combined sources
Beta strandi342 – 3454Combined sources
Beta strandi357 – 3648Combined sources
Helixi371 – 38111Combined sources
Beta strandi382 – 39211Combined sources
Helixi394 – 3963Combined sources
Helixi400 – 40910Combined sources
Beta strandi412 – 4187Combined sources
Beta strandi425 – 4317Combined sources
Helixi444 – 4463Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZFUX-ray2.00A/B242-456[»]
ProteinModelPortaliO43159.
SMRiO43159. Positions 243-454.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43159.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi65 – 706Poly-Glu

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0500.
GeneTreeiENSGT00390000006189.
HOGENOMiHOG000164004.
InParanoidiO43159.
KOiK14850.
OMAiLMGTNIR.
OrthoDBiEOG7PGDRQ.
PhylomeDBiO43159.
TreeFamiTF313749.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR007823. Methyltransferase-rel.
IPR029063. SAM-dependent_MTases-like.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PANTHERiPTHR12787. PTHR12787. 1 hit.
PfamiPF05148. Methyltransf_8. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

O43159-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFEEPEWAEA APVAAGLGPV ISRPPPAASS QNKGSKRRQL LATLRALEAA
60 70 80 90 100
SLSQHPPSLC ISDSEEEEEE RKKKCPKKAS FASASAEVGK KGKKKCQKQG
110 120 130 140 150
PPCSDSEEEV ERKKKCHKQA LVGSDSAEDE KRKRKCQKHA PINSAQHLDN
160 170 180 190 200
VDQTGPKAWK GSTTNDPPKQ SPGSTSPKPP HTLSRKQWRN RQKNKRRCKN
210 220 230 240 250
KFQPPQVPDQ APAEAPTEKT EVSPVPRTDS HEARAGALRA RMAQRLDGAR
260 270 280 290 300
FRYLNEQLYS GPSSAAQRLF QEDPEAFLLY HRGFQSQVKK WPLQPVDRIA
310 320 330 340 350
RDLRQRPASL VVADFGCGDC RLASSIRNPV HCFDLASLDP RVTVCDMAQV
360 370 380 390 400
PLEDESVDVA VFCLSLMGTN IRDFLEEANR VLKPGGLLKV AEVSSRFEDV
410 420 430 440 450
RTFLRAVTKL GFKIVSKDLT NSHFFLFDFQ KTGPPLVGPK AQLSGLQLQP

CLYKRR
Length:456
Mass (Da):50,715
Last modified:April 26, 2005 - v2
Checksum:i49251E2A488DA1C4
GO

Sequence cautioni

The sequence BAA23705.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti145 – 1451A → P.
Corresponds to variant rs11040934 [ dbSNP | Ensembl ].
VAR_051034
Natural varianti329 – 3291P → S.
Corresponds to variant rs17834692 [ dbSNP | Ensembl ].
VAR_051035

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87060 mRNA. Translation: BAB46916.1.
AB007869 mRNA. Translation: BAA23705.1. Different initiation.
BC001071 mRNA. Translation: AAH01071.1.
CCDSiCCDS31411.1.
RefSeqiNP_056139.1. NM_015324.3.
UniGeneiHs.652255.

Genome annotation databases

EnsembliENST00000254605; ENSP00000254605; ENSG00000132275.
GeneIDi23378.
KEGGihsa:23378.
UCSCiuc001med.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87060 mRNA. Translation: BAB46916.1 .
AB007869 mRNA. Translation: BAA23705.1 . Different initiation.
BC001071 mRNA. Translation: AAH01071.1 .
CCDSi CCDS31411.1.
RefSeqi NP_056139.1. NM_015324.3.
UniGenei Hs.652255.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZFU X-ray 2.00 A/B 242-456 [» ]
ProteinModelPortali O43159.
SMRi O43159. Positions 243-454.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116954. 12 interactions.
DIPi DIP-46686N.
IntActi O43159. 4 interactions.
MINTi MINT-4526401.
STRINGi 9606.ENSP00000254605.

PTM databases

PhosphoSitei O43159.

2D gel databases

SWISS-2DPAGE O43159.

Proteomic databases

MaxQBi O43159.
PaxDbi O43159.
PeptideAtlasi O43159.
PRIDEi O43159.

Protocols and materials databases

DNASUi 23378.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000254605 ; ENSP00000254605 ; ENSG00000132275 .
GeneIDi 23378.
KEGGi hsa:23378.
UCSCi uc001med.3. human.

Organism-specific databases

CTDi 23378.
GeneCardsi GC11M006577.
HGNCi HGNC:29030. RRP8.
HPAi HPA038487.
neXtProti NX_O43159.
PharmGKBi PA164725550.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0500.
GeneTreei ENSGT00390000006189.
HOGENOMi HOG000164004.
InParanoidi O43159.
KOi K14850.
OMAi LMGTNIR.
OrthoDBi EOG7PGDRQ.
PhylomeDBi O43159.
TreeFami TF313749.

Enzyme and pathway databases

Reactomei REACT_200827. SIRT1 negatively regulates rRNA Expression.

Miscellaneous databases

EvolutionaryTracei O43159.
GeneWikii KIAA0409.
GenomeRNAii 23378.
NextBioi 45466.
PROi O43159.

Gene expression databases

Bgeei O43159.
CleanExi HS_KIAA0409.
ExpressionAtlasi O43159. baseline and differential.
Genevestigatori O43159.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR007823. Methyltransferase-rel.
IPR029063. SAM-dependent_MTases-like.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view ]
PANTHERi PTHR12787. PTHR12787. 1 hit.
Pfami PF05148. Methyltransf_8. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel gene which is expressed in the human brain."
    Yazaki M., Takayama K., Matsumoto K., Yoshimoto M.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
    Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-64; SER-171 AND SER-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62 AND SER-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 242-456 IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE, IDENTIFICATION IN THE ENOSC COMPLEX, FUNCTION, SUBCELLULAR LOCATION, H3K9ME2-BINDING.

Entry informationi

Entry nameiRRP8_HUMAN
AccessioniPrimary (citable) accession number: O43159
Secondary accession number(s): Q7KZ78, Q9BVM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: November 26, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3