Reviewed, UniProtKB/Swiss-Prot O43143 (DHX15_HUMAN)

Names and origin

Protein names

Recommended name:
    Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
    EC=3.6.1.-
Alternative name(s):
    DEAH box protein 15
    ATP-dependent RNA helicase #46

Gene names

Name:	DHX15
Synonyms:	DBP1, DDX15

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA By similarity.
Subunit structure	Interacts with SSB/La. Ref.2
Subcellular location	Nucleus. Ref.2
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred by curator. Source: HGNC mRNA processing Traceable author statement. Source: ProtInc
Cellular component	U12-type spliceosomal complex Inferred from direct assay. Source: HGNC
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA helicase activity Traceable author statement. Source: ProtInc nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 795	795	Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15		PRO_0000055139
Regions
	Domain	147 – 313	167	Helicase ATP-binding
	Domain	338 – 518	181	Helicase C-terminal
	Nucleotide binding	160 – 167	8	ATP By similarity
	Motif	260 – 263	4	DEAH box
Amino acid modifications
	Modified residue	132	1	N6-acetyllysine Ref.6
	Modified residue	476	1	Phosphothreonine Ref.4
	Modified residue	488	1	N6-acetyllysine Ref.6
Experimental info
	Sequence conflict	151	1	V → G in BAA23987. Ref.1
	Sequence conflict	172 – 173	2	QW → HR in BAA23987. Ref.1
	Sequence conflict	232 – 234	3	ILK → FFM in BAA23987. Ref.1
	Sequence conflict	786 – 795	10	KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O43143-1 [UniParc]. Last modified February 21, 2001. Version 2. Checksum: 9A21FBE0051CCAA9	FASTA	795	90,933
	10 20 30 40 50 60 MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE REREKEKEKE 70 80 90 100 110 120 LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS THAGHAGHTS LPQCINPFTN 130 140 150 160 170 180 LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS 190 200 210 220 230 240 LPGPKRGVAC TQPRRVAAMS VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM 250 260 270 280 290 300 LLREAMNDPL LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF 310 320 330 340 350 360 QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG DLLLFLTGQE 370 380 390 400 410 420 EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI FEPPPPKKQN GAIGRKVVVS 430 440 450 460 470 480 TNIAETSLTI DGVVFVIDPG FAKQKVYNPR IRVESLLVTA ISKASAQQRA GRAGRTRPGK 490 500 510 520 530 540 CFRLYTEKAY KTEMQDNTYP EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL 550 560 570 580 590 600 ELLNYLAALN DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF 610 620 630 640 650 660 VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN YRSLMSADNV 670 680 690 700 710 720 RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ VAHLERTGHY LTVKDNQVVQ 730 740 750 760 770 780 LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL 790 DRIIAKLQSK EYSQY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family." Imamura O., Sugawara M., Furuichi Y. Biochem. Biophys. Res. Commun. 240:335-340(1997) [PubMed: 9388478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase." Fouraux M.A., Kolkman M.J.M., Van der Heijden A., De Jong A.S., Van Venrooij W.J., Pruijn G.J.M. RNA 8:1428-1443(2002) [PubMed: 12458796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SSB, SUBCELLULAR LOCATION. Tissue: Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[4]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-476, MASS SPECTROMETRY. Tissue: Epithelium.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-132 AND LYS-488, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB001636 mRNA. Translation: BAA23987.1. AF279891 mRNA. Translation: AAF90182.1. BC035974 mRNA. Translation: AAH35974.1.
IPI	IPI00396435.
PIR	JC5785.
RefSeq	NP_001349.2.
UniGene	Hs.696074
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O43143.
PTM databases
PhosphoSite	O43143.
2-D gel databases
SWISS-2DPAGE	O43143.
Proteomic databases
PRIDE	O43143.
Genome annotation databases
Ensembl	ENST00000336812; ENSP00000336741; ENSG00000109606; Homo sapiens. [Genome view]
GeneID	1665.
KEGG	hsa:1665.
UCSC	uc003gqx.1. human.
Organism-specific databases
CTD	1665.
GeneCards	GC04M024138.
HGNC	HGNC:2738. DHX15.
MIM	603403. gene.
PharmGKB	PA27204.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O43143.
HOVERGEN	O43143.
OMA	VIYNEFV.
Gene expression databases
ArrayExpress	O43143.
Bgee	O43143.
CleanEx	HS_DHX15.
Genevestigator	O43143.
GermOnline	ENSG00000109606. Homo sapiens.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR011709. DUF1605. IPR007502. Helicase-assoc_reg. IPR014021. Helicase_SF1/SF2_ATP-bd. [Graphical view]
Pfam	PF07717. DUF1605. 1 hit. PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	6852.
SOURCE	Search...

Entry information

Entry name

DHX15_HUMAN

Accession

Primary (citable) accession number: O43143
Secondary accession number(s): Q9NQT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 21, 2001
Last modified:	November 3, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.