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Reviewed, UniProtKB/Swiss-Prot O43143 (DHX15_HUMAN)

Last modified November 3, 2009. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
    EC=3.6.1.-
Alternative name(s):
    DEAH box protein 15
    ATP-dependent RNA helicase #46
Gene names
Name: DHX15
Synonyms: DBP1, DDX15
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length795 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA By similarity.

Subunit structure

Interacts with SSB/La. Ref.2

Subcellular location

Nucleus. Ref.2

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
   LigandATP-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA splicing

Inferred by curator. Source: HGNC

mRNA processing

Traceable author statement. Source: ProtInc

   Cellular componentU12-type spliceosomal complex

Inferred from direct assay. Source: HGNC

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

RNA helicase activity

Traceable author statement. Source: ProtInc

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 795795Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
PRO_0000055139

Regions

Domain147 – 313167Helicase ATP-binding
Domain338 – 518181Helicase C-terminal
Nucleotide binding160 – 1678ATP By similarity
Motif260 – 2634DEAH box

Amino acid modifications

Modified residue1321N6-acetyllysine Ref.6
Modified residue4761Phosphothreonine Ref.4
Modified residue4881N6-acetyllysine Ref.6

Experimental info

Sequence conflict1511V → G in BAA23987. Ref.1
Sequence conflict172 – 1732QW → HR in BAA23987. Ref.1
Sequence conflict232 – 2343ILK → FFM in BAA23987. Ref.1
Sequence conflict786 – 79510KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O43143-1 [UniParc].

Last modified February 21, 2001. Version 2.
Checksum: 9A21FBE0051CCAA9

FASTA79590,933
        10         20         30         40         50         60 
MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE REREKEKEKE 

        70         80         90        100        110        120 
LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS THAGHAGHTS LPQCINPFTN 

       130        140        150        160        170        180 
LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS 

       190        200        210        220        230        240 
LPGPKRGVAC TQPRRVAAMS VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM 

       250        260        270        280        290        300 
LLREAMNDPL LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF 

       310        320        330        340        350        360 
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG DLLLFLTGQE 

       370        380        390        400        410        420 
EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI FEPPPPKKQN GAIGRKVVVS 

       430        440        450        460        470        480 
TNIAETSLTI DGVVFVIDPG FAKQKVYNPR IRVESLLVTA ISKASAQQRA GRAGRTRPGK 

       490        500        510        520        530        540 
CFRLYTEKAY KTEMQDNTYP EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL 

       550        560        570        580        590        600 
ELLNYLAALN DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF 

       610        620        630        640        650        660 
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN YRSLMSADNV 

       670        680        690        700        710        720 
RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ VAHLERTGHY LTVKDNQVVQ 

       730        740        750        760        770        780 
LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL 

       790 
DRIIAKLQSK EYSQY 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family."
Imamura O., Sugawara M., Furuichi Y.
Biochem. Biophys. Res. Commun. 240:335-340(1997) [PubMed: 9388478] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase."
Fouraux M.A., Kolkman M.J.M., Van der Heijden A., De Jong A.S., Van Venrooij W.J., Pruijn G.J.M.
RNA 8:1428-1443(2002) [PubMed: 12458796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SSB, SUBCELLULAR LOCATION.
Tissue: Placenta.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[4]"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-476, MASS SPECTROMETRY.
Tissue: Epithelium.
[5]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed: 19608861] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-132 AND LYS-488, MASS SPECTROMETRY.

Cross-references

Sequence databases

AB001636 mRNA. Translation: BAA23987.1.
AF279891 mRNA. Translation: AAF90182.1.
BC035974 mRNA. Translation: AAH35974.1.
IPIIPI00396435.
PIRJC5785.
RefSeqNP_001349.2.
UniGeneHs.696074

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO43143.

PTM databases

PhosphoSiteO43143.

2-D gel databases

SWISS-2DPAGEO43143.

Proteomic databases

PRIDEO43143.

Genome annotation databases

EnsemblENST00000336812; ENSP00000336741; ENSG00000109606; Homo sapiens. [Genome view]
GeneID1665.
KEGGhsa:1665.
UCSCuc003gqx.1. human.

Organism-specific databases

CTD1665.
GeneCardsGC04M024138.
HGNCHGNC:2738. DHX15.
MIM603403. gene.
PharmGKBPA27204.
GenAtlasSearch...

Phylogenomic databases

HOGENOMO43143.
HOVERGENO43143.
OMAVIYNEFV.

Gene expression databases

ArrayExpressO43143.
BgeeO43143.
CleanExHS_DHX15.
GenevestigatorO43143.
GermOnlineENSG00000109606. Homo sapiens.

Family and domain databases

InterProIPR003593. ATPase_AAA+_core.
IPR014001. DEAD-like_N.
IPR001650. DNA/RNA_helicase_C.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_reg.
IPR014021. Helicase_SF1/SF2_ATP-bd.
[Graphical view]
PfamPF07717. DUF1605. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
PROSITEPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio6852.
SOURCESearch...

Entry information

Entry nameDHX15_HUMAN
AccessionPrimary (citable) accession number: O43143
Secondary accession number(s): Q9NQT7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2001
Last modified: November 3, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents