Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Gene

DHX15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi160 – 167ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • double-stranded RNA binding Source: MGI
  • RNA binding Source: UniProtKB
  • RNA helicase activity Source: ProtInc

GO - Biological processi

  • mRNA processing Source: ProtInc
  • mRNA splicing, via spliceosome Source: GO_Central
  • response to alkaloid Source: Ensembl
  • response to toxic substance Source: Ensembl
  • RNA splicing Source: HGNC

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (EC:3.6.4.131 Publication)
Alternative name(s):
ATP-dependent RNA helicase #46
DEAH box protein 15
Gene namesi
Name:DHX15
Synonyms:DBP1, DDX15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000109606.12.
HGNCiHGNC:2738. DHX15.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1665.
OpenTargetsiENSG00000109606.
PharmGKBiPA27204.

Polymorphism and mutation databases

BioMutaiDHX15.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551391 – 795Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15Add BLAST795

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei488N6-acetyllysineCombined sources1
Cross-linki786Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO43143.
MaxQBiO43143.
PaxDbiO43143.
PeptideAtlasiO43143.
PRIDEiO43143.

2D gel databases

SWISS-2DPAGEiO43143.

PTM databases

iPTMnetiO43143.
PhosphoSitePlusiO43143.
SwissPalmiO43143.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000109606.
CleanExiHS_DHX15.
GenevisibleiO43143. HS.

Organism-specific databases

HPAiHPA047047.

Interactioni

Subunit structurei

Interacts with SSB/La (PubMed:12458796). Component of the U11/U12 snRNPs that are part of the U12-type spliceosome (PubMed:15146077). Identified in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient. Interacts with TFIP11; indicative for a recruitment to the IL complex (PubMed:19103666). Interacts with GPATCH2 (PubMed:19432882).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi108029. 191 interactors.
CORUMiO43143.
DIPiDIP-38211N.
IntActiO43143. 73 interactors.
MINTiMINT-5003827.
STRINGi9606.ENSP00000336741.

Structurei

Secondary structure

1795
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni117 – 120Combined sources4
Helixi125 – 134Combined sources10
Helixi138 – 142Combined sources5
Helixi143 – 150Combined sources8
Beta strandi154 – 160Combined sources7
Helixi166 – 179Combined sources14
Beta strandi181 – 183Combined sources3
Beta strandi187 – 193Combined sources7
Helixi195 – 208Combined sources14
Turni214 – 216Combined sources3
Beta strandi217 – 221Combined sources5
Beta strandi224 – 226Combined sources3
Beta strandi233 – 237Combined sources5
Helixi238 – 246Combined sources9
Beta strandi254 – 259Combined sources6
Helixi262 – 264Combined sources3
Helixi267 – 282Combined sources16
Beta strandi287 – 293Combined sources7
Turni301 – 305Combined sources5
Beta strandi309 – 312Combined sources4
Beta strandi319 – 322Combined sources4
Helixi331 – 345Combined sources15
Beta strandi351 – 355Combined sources5
Helixi361 – 367Combined sources7
Turni368 – 370Combined sources3
Beta strandi371 – 373Combined sources3
Beta strandi378 – 380Combined sources3
Beta strandi383 – 388Combined sources6
Helixi394 – 397Combined sources4
Beta strandi400 – 402Combined sources3
Beta strandi409 – 412Combined sources4
Beta strandi415 – 420Combined sources6
Helixi423 – 426Combined sources4
Beta strandi433 – 438Combined sources6
Beta strandi441 – 448Combined sources8
Turni449 – 452Combined sources4
Beta strandi453 – 460Combined sources8
Helixi463 – 471Combined sources9
Helixi472 – 474Combined sources3
Beta strandi475 – 477Combined sources3
Beta strandi479 – 485Combined sources7
Turni488 – 493Combined sources6
Helixi501 – 503Combined sources3
Helixi508 – 516Combined sources9
Beta strandi522 – 525Combined sources4
Helixi533 – 545Combined sources13
Helixi557 – 562Combined sources6
Beta strandi565 – 567Combined sources3
Helixi569 – 578Combined sources10
Helixi579 – 581Combined sources3
Helixi584 – 594Combined sources11
Helixi604 – 606Combined sources3
Helixi607 – 615Combined sources9
Helixi623 – 636Combined sources14
Turni637 – 639Combined sources3
Helixi641 – 646Combined sources6
Helixi651 – 670Combined sources20
Helixi684 – 697Combined sources14
Beta strandi700 – 704Combined sources5
Beta strandi710 – 712Combined sources3
Turni713 – 715Combined sources3
Beta strandi718 – 721Combined sources4
Beta strandi732 – 752Combined sources21
Helixi755 – 761Combined sources7
Turni763 – 765Combined sources3
Helixi768 – 770Combined sources3
Helixi775 – 788Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XDRX-ray2.00A110-795[»]
ProteinModelPortaliO43143.
SMRiO43143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini147 – 313Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST167
Domaini338 – 518Helicase C-terminalPROSITE-ProRule annotationAdd BLAST181

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi260 – 263DEAH box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00900000140964.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiO43143.
KOiK12820.
OMAiQWCVDFA.
OrthoDBiEOG091G030Q.
PhylomeDBiO43143.
TreeFamiTF105735.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.

Sequencei

Sequence statusi: Complete.

O43143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE
60 70 80 90 100
REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS
110 120 130 140 150
THAGHAGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL
160 170 180 190 200
VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS
210 220 230 240 250
VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL
260 270 280 290 300
LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF
310 320 330 340 350
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG
360 370 380 390 400
DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI
410 420 430 440 450
FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR
460 470 480 490 500
IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP
510 520 530 540 550
EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN
560 570 580 590 600
DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF
610 620 630 640 650
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN
660 670 680 690 700
YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ
710 720 730 740 750
VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC
760 770 780 790
TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY
Length:795
Mass (Da):90,933
Last modified:February 21, 2001 - v2
Checksum:i9A21FBE0051CCAA9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151V → G in BAA23987 (PubMed:9388478).Curated1
Sequence conflicti172 – 173QW → HR in BAA23987 (PubMed:9388478).Curated2
Sequence conflicti232 – 234ILK → FFM in BAA23987 (PubMed:9388478).Curated3
Sequence conflicti786 – 795KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987 (PubMed:9388478).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001636 mRNA. Translation: BAA23987.1.
AF279891 mRNA. Translation: AAF90182.1.
BC035974 mRNA. Translation: AAH35974.1.
CCDSiCCDS33966.1.
PIRiJC5785.
RefSeqiNP_001349.2. NM_001358.2.
UniGeneiHs.696074.

Genome annotation databases

EnsembliENST00000336812; ENSP00000336741; ENSG00000109606.
GeneIDi1665.
KEGGihsa:1665.
UCSCiuc003gqx.4. human.

Similar proteinsi

Entry informationi

Entry nameiDHX15_HUMAN
AccessioniPrimary (citable) accession number: O43143
Secondary accession number(s): Q9NQT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2001
Last modified: October 25, 2017
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families