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O43143

- DHX15_HUMAN

UniProt

O43143 - DHX15_HUMAN

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Protein

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Gene

DHX15

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 1678ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. double-stranded RNA binding Source: MGI
  4. poly(A) RNA binding Source: UniProtKB
  5. RNA helicase activity Source: ProtInc

GO - Biological processi

  1. mRNA processing Source: ProtInc
  2. RNA splicing Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase #46
DEAH box protein 15
Gene namesi
Name:DHX15
Synonyms:DBP1, DDX15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:2738. DHX15.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: HPA
  2. U12-type spliceosomal complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27204.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 795795Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15PRO_0000055139Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei488 – 4881N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO43143.
PaxDbiO43143.
PRIDEiO43143.

2D gel databases

SWISS-2DPAGEO43143.

PTM databases

PhosphoSiteiO43143.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiO43143.
CleanExiHS_DHX15.
GenevestigatoriO43143.

Organism-specific databases

HPAiHPA047047.

Interactioni

Subunit structurei

Interacts with SSB/La. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Identified in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient. Interacts with TFIP11; indicative for a recruitment to the IL complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RBM10P981752EBI-1237044,EBI-721525
RBM17Q96I252EBI-1237044,EBI-740272
RBM5P5275615EBI-1237044,EBI-714003
SF3A2Q154282EBI-1237044,EBI-2462271
SUGP1Q8IWZ82EBI-1237044,EBI-2691671

Protein-protein interaction databases

BioGridi108029. 125 interactions.
DIPiDIP-38211N.
IntActiO43143. 57 interactions.
MINTiMINT-5003827.
STRINGi9606.ENSP00000336741.

Structurei

3D structure databases

ProteinModelPortaliO43143.
SMRiO43143. Positions 115-787.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 313167Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini338 – 518181Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi260 – 2634DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1643.
GeneTreeiENSGT00760000119249.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiO43143.
KOiK12820.
OMAiFTSKDYY.
OrthoDBiEOG7FXZXR.
PhylomeDBiO43143.
TreeFamiTF105735.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43143-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE
60 70 80 90 100
REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS
110 120 130 140 150
THAGHAGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL
160 170 180 190 200
VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS
210 220 230 240 250
VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL
260 270 280 290 300
LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF
310 320 330 340 350
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG
360 370 380 390 400
DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI
410 420 430 440 450
FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR
460 470 480 490 500
IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP
510 520 530 540 550
EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN
560 570 580 590 600
DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF
610 620 630 640 650
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN
660 670 680 690 700
YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ
710 720 730 740 750
VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC
760 770 780 790
TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY
Length:795
Mass (Da):90,933
Last modified:February 21, 2001 - v2
Checksum:i9A21FBE0051CCAA9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511V → G in BAA23987. (PubMed:9388478)Curated
Sequence conflicti172 – 1732QW → HR in BAA23987. (PubMed:9388478)Curated
Sequence conflicti232 – 2343ILK → FFM in BAA23987. (PubMed:9388478)Curated
Sequence conflicti786 – 79510KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987. (PubMed:9388478)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001636 mRNA. Translation: BAA23987.1.
AF279891 mRNA. Translation: AAF90182.1.
BC035974 mRNA. Translation: AAH35974.1.
CCDSiCCDS33966.1.
PIRiJC5785.
RefSeqiNP_001349.2. NM_001358.2.
UniGeneiHs.696074.

Genome annotation databases

EnsembliENST00000336812; ENSP00000336741; ENSG00000109606.
GeneIDi1665.
KEGGihsa:1665.
UCSCiuc003gqx.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB001636 mRNA. Translation: BAA23987.1 .
AF279891 mRNA. Translation: AAF90182.1 .
BC035974 mRNA. Translation: AAH35974.1 .
CCDSi CCDS33966.1.
PIRi JC5785.
RefSeqi NP_001349.2. NM_001358.2.
UniGenei Hs.696074.

3D structure databases

ProteinModelPortali O43143.
SMRi O43143. Positions 115-787.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 108029. 125 interactions.
DIPi DIP-38211N.
IntActi O43143. 57 interactions.
MINTi MINT-5003827.
STRINGi 9606.ENSP00000336741.

PTM databases

PhosphoSitei O43143.

2D gel databases

SWISS-2DPAGE O43143.

Proteomic databases

MaxQBi O43143.
PaxDbi O43143.
PRIDEi O43143.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000336812 ; ENSP00000336741 ; ENSG00000109606 .
GeneIDi 1665.
KEGGi hsa:1665.
UCSCi uc003gqx.3. human.

Organism-specific databases

CTDi 1665.
GeneCardsi GC04M024519.
HGNCi HGNC:2738. DHX15.
HPAi HPA047047.
MIMi 603403. gene.
neXtProti NX_O43143.
PharmGKBi PA27204.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1643.
GeneTreei ENSGT00760000119249.
HOGENOMi HOG000175261.
HOVERGENi HBG039428.
InParanoidi O43143.
KOi K12820.
OMAi FTSKDYY.
OrthoDBi EOG7FXZXR.
PhylomeDBi O43143.
TreeFami TF105735.

Miscellaneous databases

GeneWikii DHX15.
GenomeRNAii 1665.
NextBioi 6852.
PROi O43143.
SOURCEi Search...

Gene expression databases

Bgeei O43143.
CleanExi HS_DHX15.
Genevestigatori O43143.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family."
    Imamura O., Sugawara M., Furuichi Y.
    Biochem. Biophys. Res. Commun. 240:335-340(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase."
    Fouraux M.A., Kolkman M.J.M., Van der Heijden A., De Jong A.S., Van Venrooij W.J., Pruijn G.J.M.
    RNA 8:1428-1443(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SSB, SUBCELLULAR LOCATION.
    Tissue: Placenta.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  4. "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome."
    Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S., Tuschl T., Luehrmann R.
    RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH THE U11/U12 SPLICEOSOME, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Isolation and characterization of post-splicing lariat-intron complexes."
    Yoshimoto R., Kataoka N., Okawa K., Ohno M.
    Nucleic Acids Res. 37:891-902(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE INTRON LARGE COMPLEX, INTERACTION WITH TFIP11.
  6. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."
    Ahmad Y., Boisvert F.M., Lundberg E., Uhlen M., Lamond A.I.
    Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDHX15_HUMAN
AccessioniPrimary (citable) accession number: O43143
Secondary accession number(s): Q9NQT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2001
Last modified: October 29, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3