Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

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O43143 (DHX15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 131. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
EC=3.6.4.13

Alternative name(s):
ATP-dependent RNA helicase #46
DEAH box protein 15

Gene names

Name:	DHX15
Synonyms:	DBP1, DDX15

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	Interacts with SSB/La. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Ref.2 Ref.4
Subcellular location	Nucleus. Nucleus › nucleolus Ref.2 Ref.7.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred by curator Ref.4. Source: HGNC mRNA processing Traceable author statement PubMed 7935475. Source: ProtInc
Cellular_component	U12-type spliceosomal complex Inferred from direct assay Ref.4. Source: HGNC nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA helicase activity Traceable author statement PubMed 7935475. Source: ProtInc nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 795

795

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

PRO_0000055139

Regions

Domain

147 – 313

167

Helicase ATP-binding

Domain

338 – 518

181

Helicase C-terminal

Nucleotide binding

160 – 167

ATP By similarity

Motif

260 – 263

DEAH box

Amino acid modifications

Modified residue

132

N6-acetyllysine Ref.5

Modified residue

488

N6-acetyllysine Ref.5

Experimental info

Sequence conflict

151

V → G in BAA23987. Ref.1

Sequence conflict

172 – 173

QW → HR in BAA23987. Ref.1

Sequence conflict

232 – 234

ILK → FFM in BAA23987. Ref.1

Sequence conflict

786 – 795

KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O43143 [UniParc].

Last modified February 21, 2001. Version 2.
Checksum: 9A21FBE0051CCAA9
Show »

FASTA

795

90,933

        10         20         30         40         50         60 
MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE REREKEKEKE 

        70         80         90        100        110        120 
LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS THAGHAGHTS LPQCINPFTN 

       130        140        150        160        170        180 
LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS 

       190        200        210        220        230        240 
LPGPKRGVAC TQPRRVAAMS VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM 

       250        260        270        280        290        300 
LLREAMNDPL LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF 

       310        320        330        340        350        360 
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG DLLLFLTGQE 

       370        380        390        400        410        420 
EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI FEPPPPKKQN GAIGRKVVVS 

       430        440        450        460        470        480 
TNIAETSLTI DGVVFVIDPG FAKQKVYNPR IRVESLLVTA ISKASAQQRA GRAGRTRPGK 

       490        500        510        520        530        540 
CFRLYTEKAY KTEMQDNTYP EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL 

       550        560        570        580        590        600 
ELLNYLAALN DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF 

       610        620        630        640        650        660 
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN YRSLMSADNV 

       670        680        690        700        710        720 
RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ VAHLERTGHY LTVKDNQVVQ 

       730        740        750        760        770        780 
LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL 

       790 
DRIIAKLQSK EYSQY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family." Imamura O., Sugawara M., Furuichi Y. Biochem. Biophys. Res. Commun. 240:335-340(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase." Fouraux M.A., Kolkman M.J.M., Van der Heijden A., De Jong A.S., Van Venrooij W.J., Pruijn G.J.M. RNA 8:1428-1443(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SSB, SUBCELLULAR LOCATION. Tissue: Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[4]	"The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome." Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S., Tuschl T., Luehrmann R. RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH THE U11/U12 SPLICEOSOME, MASS SPECTROMETRY.
[5]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-132 AND LYS-488, MASS SPECTROMETRY.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization." Ahmad Y., Boisvert F.M., Lundberg E., Uhlen M., Lamond A.I. Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB001636 mRNA. Translation: BAA23987.1. AF279891 mRNA. Translation: AAF90182.1. BC035974 mRNA. Translation: AAH35974.1.
IPI	IPI00396435.
PIR	JC5785.
RefSeq	NP_001349.2. NM_001358.2.
UniGene	Hs.696074.
3D structure databases
ProteinModelPortal	O43143.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-38211N.
IntAct	O43143. 23 interactions.
MINT	MINT-5003827.
STRING	9606.ENSP00000336741.
PTM databases
PhosphoSite	O43143.
2D gel databases
SWISS-2DPAGE	O43143.
Proteomic databases
PaxDb	O43143.
PRIDE	O43143.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336812; ENSP00000336741; ENSG00000109606.
GeneID	1665.
KEGG	hsa:1665.
UCSC	uc003gqx.3. human.
Organism-specific databases
CTD	1665.
GeneCards	GC04M024519.
HGNC	HGNC:2738. DHX15.
HPA	HPA047047.
MIM	603403. gene.
neXtProt	NX_O43143.
PharmGKB	PA27204.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1643.
HOGENOM	HOG000175261.
HOVERGEN	HBG039428.
InParanoid	O43143.
KO	K12820.
OMA	TVLQIHA.
OrthoDB	EOG4K6G3N.
Gene expression databases
ArrayExpress	O43143.
Bgee	O43143.
CleanEx	HS_DHX15.
Genevestigator	O43143.
GermOnline	ENSG00000109606. Homo sapiens.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. PF07717. OB_NTP_bind. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. SM00847. HA2. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1665.
NextBio	6852.
SOURCE	Search...

Entry information

Entry name

DHX15_HUMAN

Accession

Primary (citable) accession number: O43143
Secondary accession number(s): Q9NQT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 21, 2001
Last modified:	May 29, 2013
This is version 131 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents