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O43143

- DHX15_HUMAN

UniProt

O43143 - DHX15_HUMAN

Protein

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Gene

DHX15

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 2 (21 Feb 2001)
      Previous versions | rss
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    Functioni

    Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi160 – 1678ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. double-stranded RNA binding Source: MGI
    4. poly(A) RNA binding Source: UniProtKB
    5. protein binding Source: IntAct
    6. RNA helicase activity Source: ProtInc

    GO - Biological processi

    1. mRNA processing Source: ProtInc
    2. RNA splicing Source: HGNC

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (EC:3.6.4.13)
    Alternative name(s):
    ATP-dependent RNA helicase #46
    DEAH box protein 15
    Gene namesi
    Name:DHX15
    Synonyms:DBP1, DDX15
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:2738. DHX15.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell
    2. nucleus Source: HPA
    3. U12-type spliceosomal complex Source: HGNC

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27204.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 795795Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15PRO_0000055139Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei488 – 4881N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO43143.
    PaxDbiO43143.
    PRIDEiO43143.

    2D gel databases

    SWISS-2DPAGEO43143.

    PTM databases

    PhosphoSiteiO43143.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    BgeeiO43143.
    CleanExiHS_DHX15.
    GenevestigatoriO43143.

    Organism-specific databases

    HPAiHPA047047.

    Interactioni

    Subunit structurei

    Interacts with SSB/La. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Identified in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient. Interacts with TFIP11; indicative for a recruitment to the IL complex.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RBM10P981752EBI-1237044,EBI-721525
    RBM17Q96I252EBI-1237044,EBI-740272
    RBM5P5275615EBI-1237044,EBI-714003
    SF3A2Q154282EBI-1237044,EBI-2462271
    SUGP1Q8IWZ82EBI-1237044,EBI-2691671

    Protein-protein interaction databases

    BioGridi108029. 120 interactions.
    DIPiDIP-38211N.
    IntActiO43143. 57 interactions.
    MINTiMINT-5003827.
    STRINGi9606.ENSP00000336741.

    Structurei

    3D structure databases

    ProteinModelPortaliO43143.
    SMRiO43143. Positions 115-787.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini147 – 313167Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini338 – 518181Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi260 – 2634DEAH box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    HOGENOMiHOG000175261.
    HOVERGENiHBG039428.
    InParanoidiO43143.
    KOiK12820.
    OMAiFTSKDYY.
    OrthoDBiEOG7FXZXR.
    PhylomeDBiO43143.
    TreeFamiTF105735.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O43143-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE    50
    REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS 100
    THAGHAGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL 150
    VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS 200
    VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL 250
    LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF 300
    QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG 350
    DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI 400
    FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR 450
    IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP 500
    EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN 550
    DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF 600
    VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN 650
    YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ 700
    VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC 750
    TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY 795
    Length:795
    Mass (Da):90,933
    Last modified:February 21, 2001 - v2
    Checksum:i9A21FBE0051CCAA9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti151 – 1511V → G in BAA23987. (PubMed:9388478)Curated
    Sequence conflicti172 – 1732QW → HR in BAA23987. (PubMed:9388478)Curated
    Sequence conflicti232 – 2343ILK → FFM in BAA23987. (PubMed:9388478)Curated
    Sequence conflicti786 – 79510KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987. (PubMed:9388478)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001636 mRNA. Translation: BAA23987.1.
    AF279891 mRNA. Translation: AAF90182.1.
    BC035974 mRNA. Translation: AAH35974.1.
    CCDSiCCDS33966.1.
    PIRiJC5785.
    RefSeqiNP_001349.2. NM_001358.2.
    UniGeneiHs.696074.

    Genome annotation databases

    EnsembliENST00000336812; ENSP00000336741; ENSG00000109606.
    GeneIDi1665.
    KEGGihsa:1665.
    UCSCiuc003gqx.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001636 mRNA. Translation: BAA23987.1 .
    AF279891 mRNA. Translation: AAF90182.1 .
    BC035974 mRNA. Translation: AAH35974.1 .
    CCDSi CCDS33966.1.
    PIRi JC5785.
    RefSeqi NP_001349.2. NM_001358.2.
    UniGenei Hs.696074.

    3D structure databases

    ProteinModelPortali O43143.
    SMRi O43143. Positions 115-787.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108029. 120 interactions.
    DIPi DIP-38211N.
    IntActi O43143. 57 interactions.
    MINTi MINT-5003827.
    STRINGi 9606.ENSP00000336741.

    PTM databases

    PhosphoSitei O43143.

    2D gel databases

    SWISS-2DPAGE O43143.

    Proteomic databases

    MaxQBi O43143.
    PaxDbi O43143.
    PRIDEi O43143.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000336812 ; ENSP00000336741 ; ENSG00000109606 .
    GeneIDi 1665.
    KEGGi hsa:1665.
    UCSCi uc003gqx.3. human.

    Organism-specific databases

    CTDi 1665.
    GeneCardsi GC04M024519.
    HGNCi HGNC:2738. DHX15.
    HPAi HPA047047.
    MIMi 603403. gene.
    neXtProti NX_O43143.
    PharmGKBi PA27204.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1643.
    HOGENOMi HOG000175261.
    HOVERGENi HBG039428.
    InParanoidi O43143.
    KOi K12820.
    OMAi FTSKDYY.
    OrthoDBi EOG7FXZXR.
    PhylomeDBi O43143.
    TreeFami TF105735.

    Miscellaneous databases

    GeneWikii DHX15.
    GenomeRNAii 1665.
    NextBioi 6852.
    PROi O43143.
    SOURCEi Search...

    Gene expression databases

    Bgeei O43143.
    CleanExi HS_DHX15.
    Genevestigatori O43143.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family."
      Imamura O., Sugawara M., Furuichi Y.
      Biochem. Biophys. Res. Commun. 240:335-340(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase."
      Fouraux M.A., Kolkman M.J.M., Van der Heijden A., De Jong A.S., Van Venrooij W.J., Pruijn G.J.M.
      RNA 8:1428-1443(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SSB, SUBCELLULAR LOCATION.
      Tissue: Placenta.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    4. "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome."
      Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S., Tuschl T., Luehrmann R.
      RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH THE U11/U12 SPLICEOSOME, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Isolation and characterization of post-splicing lariat-intron complexes."
      Yoshimoto R., Kataoka N., Okawa K., Ohno M.
      Nucleic Acids Res. 37:891-902(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE INTRON LARGE COMPLEX, INTERACTION WITH TFIP11.
    6. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."
      Ahmad Y., Boisvert F.M., Lundberg E., Uhlen M., Lamond A.I.
      Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDHX15_HUMAN
    AccessioniPrimary (citable) accession number: O43143
    Secondary accession number(s): Q9NQT7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: February 21, 2001
    Last modified: October 1, 2014
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3