O43143 (DHX15_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 131.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 EC=3.6.4.13 Alternative name(s): ATP-dependent RNA helicase #46 DEAH box protein 15 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Reference proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 795 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA By similarity. |
| Catalytic activity | ATP + H2O = ADP + phosphate. |
| Subunit structure | Interacts with SSB/La. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Ref.2 Ref.4 |
| Subcellular location | |
| Tissue specificity | Ubiquitous. |
| Sequence similarities | Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | mRNA processing mRNA splicing |
| Cellular component | Nucleus |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Helicase Hydrolase |
| PTM | Acetylation |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | RNA splicing Inferred by curator Ref.4. Source: HGNC mRNA processingTraceable author statement PubMed 7935475. Source: ProtInc |
| Cellular_component | U12-type spliceosomal complex Inferred from direct assay Ref.4. Source: HGNC nucleolusInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activityInferred from electronic annotation. Source: InterPro RNA helicase activityTraceable author statement PubMed 7935475. Source: ProtInc nucleic acid bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 795 | 795 | Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 | PRO_0000055139 | |||||
Regions | |||||||||
| Domain | 147 – 313 | 167 | Helicase ATP-binding | ||||||
| Domain | 338 – 518 | 181 | Helicase C-terminal | ||||||
| Nucleotide binding | 160 – 167 | 8 | ATP By similarity | ||||||
| Motif | 260 – 263 | 4 | DEAH box | ||||||
Amino acid modifications | |||||||||
| Modified residue | 132 | 1 | N6-acetyllysine Ref.5 | ||||||
| Modified residue | 488 | 1 | N6-acetyllysine Ref.5 | ||||||
Experimental info | |||||||||
| Sequence conflict | 151 | 1 | V → G in BAA23987. Ref.1 | ||||||
| Sequence conflict | 172 – 173 | 2 | QW → HR in BAA23987. Ref.1 | ||||||
| Sequence conflict | 232 – 234 | 3 | ILK → FFM in BAA23987. Ref.1 | ||||||
| Sequence conflict | 786 – 795 | 10 | KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family." Imamura O., Sugawara M., Furuichi Y. Biochem. Biophys. Res. Commun. 240:335-340(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase." Fouraux M.A., Kolkman M.J.M., Van der Heijden A., De Jong A.S., Van Venrooij W.J., Pruijn G.J.M. RNA 8:1428-1443(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SSB, SUBCELLULAR LOCATION. Tissue: Placenta. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate. |
| [4] | "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome." Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S., Tuschl T., Luehrmann R. RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH THE U11/U12 SPLICEOSOME, MASS SPECTROMETRY. |
| [5] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-132 AND LYS-488, MASS SPECTROMETRY. |
| [6] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| [7] | "Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization." Ahmad Y., Boisvert F.M., Lundberg E., Uhlen M., Lamond A.I. Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB001636 mRNA. Translation: BAA23987.1. AF279891 mRNA. Translation: AAF90182.1. BC035974 mRNA. Translation: AAH35974.1. |
| IPI | IPI00396435. |
| PIR | JC5785. |
| RefSeq | NP_001349.2. NM_001358.2. |
| UniGene | Hs.696074. |
3D structure databases | |
| ProteinModelPortal | O43143. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-38211N. |
| IntAct | O43143. 23 interactions. |
| MINT | MINT-5003827. |
| STRING | 9606.ENSP00000336741. |
PTM databases | |
| PhosphoSite | O43143. |
2D gel databases | |
| SWISS-2DPAGE | O43143. |
Proteomic databases | |
| PaxDb | O43143. |
| PRIDE | O43143. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000336812; ENSP00000336741; ENSG00000109606. |
| GeneID | 1665. |
| KEGG | hsa:1665. |
| UCSC | uc003gqx.3. human. |
Organism-specific databases | |
| CTD | 1665. |
| GeneCards | GC04M024519. |
| HGNC | HGNC:2738. DHX15. |
| HPA | HPA047047. |
| MIM | 603403. gene. |
| neXtProt | NX_O43143. |
| PharmGKB | PA27204. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | COG1643. |
| HOGENOM | HOG000175261. |
| HOVERGEN | HBG039428. |
| InParanoid | O43143. |
| KO | K12820. |
| OMA | TVLQIHA. |
| OrthoDB | EOG4K6G3N. |
Gene expression databases | |
| ArrayExpress | O43143. |
| Bgee | O43143. |
| CleanEx | HS_DHX15. |
| Genevestigator | O43143. |
| GermOnline | ENSG00000109606. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR003593. AAA+_ATPase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. [Graphical view] |
| Pfam | PF00270. DEAD. 1 hit. PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. PF07717. OB_NTP_bind. 1 hit. [Graphical view] |
| SMART | SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. SM00847. HA2. 1 hit. SM00490. HELICc. 1 hit. [Graphical view] |
| SUPFAM | SSF52540. SSF52540. 1 hit. |
| PROSITE | PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| GenomeRNAi | 1665. |
| NextBio | 6852. |
| SOURCE | Search... |
Entry information
| Entry name | DHX15_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O43143 Secondary accession number(s): Q9NQT7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with
