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O43143 (DHX15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

EC=3.6.4.13
Alternative name(s):
ATP-dependent RNA helicase #46
DEAH box protein 15
Gene names
Name:DHX15
Synonyms:DBP1, DDX15
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length795 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. Ref.5

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with SSB/La. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Identified in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient. Interacts with TFIP11; indicative for a recruitment to the IL complex. Ref.2 Ref.4 Ref.5

Subcellular location

Nucleus. Nucleusnucleolus Ref.2 Ref.8.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 795795Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
PRO_0000055139

Regions

Domain147 – 313167Helicase ATP-binding
Domain338 – 518181Helicase C-terminal
Nucleotide binding160 – 1678ATP By similarity
Motif260 – 2634DEAH box

Amino acid modifications

Modified residue4881N6-acetyllysine Ref.6

Experimental info

Sequence conflict1511V → G in BAA23987. Ref.1
Sequence conflict172 – 1732QW → HR in BAA23987. Ref.1
Sequence conflict232 – 2343ILK → FFM in BAA23987. Ref.1
Sequence conflict786 – 79510KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O43143 [UniParc].

Last modified February 21, 2001. Version 2.
Checksum: 9A21FBE0051CCAA9

FASTA79590,933
        10         20         30         40         50         60 
MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE REREKEKEKE 

        70         80         90        100        110        120 
LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS THAGHAGHTS LPQCINPFTN 

       130        140        150        160        170        180 
LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS 

       190        200        210        220        230        240 
LPGPKRGVAC TQPRRVAAMS VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM 

       250        260        270        280        290        300 
LLREAMNDPL LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF 

       310        320        330        340        350        360 
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG DLLLFLTGQE 

       370        380        390        400        410        420 
EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI FEPPPPKKQN GAIGRKVVVS 

       430        440        450        460        470        480 
TNIAETSLTI DGVVFVIDPG FAKQKVYNPR IRVESLLVTA ISKASAQQRA GRAGRTRPGK 

       490        500        510        520        530        540 
CFRLYTEKAY KTEMQDNTYP EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL 

       550        560        570        580        590        600 
ELLNYLAALN DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF 

       610        620        630        640        650        660 
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN YRSLMSADNV 

       670        680        690        700        710        720 
RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ VAHLERTGHY LTVKDNQVVQ 

       730        740        750        760        770        780 
LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL 

       790 
DRIIAKLQSK EYSQY 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a putative human RNA helicase gene of the DEAH-box protein family."
Imamura O., Sugawara M., Furuichi Y.
Biochem. Biophys. Res. Commun. 240:335-340(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase."
Fouraux M.A., Kolkman M.J.M., Van der Heijden A., De Jong A.S., Van Venrooij W.J., Pruijn G.J.M.
RNA 8:1428-1443(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SSB, SUBCELLULAR LOCATION.
Tissue: Placenta.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[4]"The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome."
Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S., Tuschl T., Luehrmann R.
RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH THE U11/U12 SPLICEOSOME, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"Isolation and characterization of post-splicing lariat-intron complexes."
Yoshimoto R., Kataoka N., Okawa K., Ohno M.
Nucleic Acids Res. 37:891-902(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE INTRON LARGE COMPLEX, INTERACTION WITH TFIP11.
[6]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."
Ahmad Y., Boisvert F.M., Lundberg E., Uhlen M., Lamond A.I.
Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB001636 mRNA. Translation: BAA23987.1.
AF279891 mRNA. Translation: AAF90182.1.
BC035974 mRNA. Translation: AAH35974.1.
PIRJC5785.
RefSeqNP_001349.2. NM_001358.2.
UniGeneHs.696074.

3D structure databases

ProteinModelPortalO43143.
SMRO43143. Positions 115-787.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108029. 115 interactions.
DIPDIP-38211N.
IntActO43143. 41 interactions.
MINTMINT-5003827.
STRING9606.ENSP00000336741.

PTM databases

PhosphoSiteO43143.

2D gel databases

SWISS-2DPAGEO43143.

Proteomic databases

PaxDbO43143.
PRIDEO43143.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336812; ENSP00000336741; ENSG00000109606.
GeneID1665.
KEGGhsa:1665.
UCSCuc003gqx.3. human.

Organism-specific databases

CTD1665.
GeneCardsGC04M024519.
HGNCHGNC:2738. DHX15.
HPAHPA047047.
MIM603403. gene.
neXtProtNX_O43143.
PharmGKBPA27204.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1643.
HOGENOMHOG000175261.
HOVERGENHBG039428.
InParanoidO43143.
KOK12820.
OMAFTSKDYY.
OrthoDBEOG7FXZXR.
PhylomeDBO43143.
TreeFamTF105735.

Gene expression databases

BgeeO43143.
CleanExHS_DHX15.
GenevestigatorO43143.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiDHX15.
GenomeRNAi1665.
NextBio6852.
PROO43143.
SOURCESearch...

Entry information

Entry nameDHX15_HUMAN
AccessionPrimary (citable) accession number: O43143
Secondary accession number(s): Q9NQT7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2001
Last modified: April 16, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM