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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

PCK1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.

Pathway:igluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi244 – 2518ATPSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP] (EC:4.1.1.49)
Gene namesi
Name:PCK1
Ordered Locus Names:KLLA0A00484g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598 Componenti: Chromosome A

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Phosphoenolpyruvate carboxykinase [ATP]PRO_0000203873Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_451019.1.

Structurei

3D structure databases

ProteinModelPortaliO43112.
SMRiO43112. Positions 26-543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1866.
HOGENOMiHOG000271471.
InParanoidiO43112.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG7MKWFS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPTKTQNSA EENIRAELGL SAEVVTIRRN APAALLYEDA LKERDTAISN
60 70 80 90 100
AGALIAYSGD KTGRSPRDKR IVEEETSKDN VWWGPVNKPC SERTWEINRE
110 120 130 140 150
RAADYLRTRD HIYIVDAYAG WDPRYRIKVR VVCARAYHAL FMTNMLIRPS
160 170 180 190 200
KEELENFGEP DFTVWNAGQF PANTHTSGMT SKTTVEINFK QMEMVILGTE
210 220 230 240 250
YAGEMKKGIF TVMFYLMPVN HNVLTLHSSA NQGIQDNDVT LFFGLSGTGK
260 270 280 290 300
TTLSADPHRL LIGDDEHCWS DHGVFNIEGG CYAKCLGLSA EKEPEIFNAI
310 320 330 340 350
KFGSVLENII YDPNTREVDY EDSTITENTR CAYPIEYIPS AKIPCLADHH
360 370 380 390 400
PKNIVLLTCD ASGVLPPVSK LTPDQVMYHF ISGYTSKMAG TEQGVTEPEA
410 420 430 440 450
TFSSCFGQPF LSLHPMKYAT MLAEKMAEHN ANAWLINTGW TGSSYVAGGK
460 470 480 490 500
RCPLKYTRAI LDAIHDGSLA KEEYEVLPIF NLQIPKAVGD KVPASLLNPS
510 520 530 540
KNWAEGEAKY TSNVKSLANL FVENFKTYQD KATEQVLAAG PQL
Length:543
Mass (Da):60,429
Last modified:September 27, 2004 - v2
Checksum:i35CD60147227B2A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2361D → G in AAC27661 (PubMed:9717242).Curated
Sequence conflicti255 – 2551A → G in AAC27661 (PubMed:9717242).Curated
Sequence conflicti500 – 5001S → I in AAC27661 (PubMed:9717242).Curated
Sequence conflicti509 – 5091K → N in AAC27661 (PubMed:9717242).Curated
Sequence conflicti520 – 5201L → W in AAC27661 (PubMed:9717242).Curated
Sequence conflicti523 – 5231E → K in AAC27661 (PubMed:9717242).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88575 Genomic DNA. Translation: AAC27661.1.
CR382121 Genomic DNA. Translation: CAH02607.1.
RefSeqiXP_451019.1. XM_451019.1.

Genome annotation databases

GeneIDi2896341.
KEGGikla:KLLA0A00484g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88575 Genomic DNA. Translation: AAC27661.1.
CR382121 Genomic DNA. Translation: CAH02607.1.
RefSeqiXP_451019.1. XM_451019.1.

3D structure databases

ProteinModelPortaliO43112.
SMRiO43112. Positions 26-543.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_451019.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2896341.
KEGGikla:KLLA0A00484g.

Phylogenomic databases

eggNOGiCOG1866.
HOGENOMiHOG000271471.
InParanoidiO43112.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG7MKWFS.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase from Kluyveromyces lactis."
    Kitamoto H.K., Ohmomo S., Iimura Y.
    Yeast 14:963-967(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Entry informationi

Entry nameiPCKA_KLULA
AccessioniPrimary (citable) accession number: O43112
Secondary accession number(s): Q6CYH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 27, 2004
Last modified: June 24, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.