O43112 (PCKA_KLULA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]
EC=4.1.1.49

Gene names

Name:	PCK1
Ordered Locus Names:	KLLA0A00484g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces › Kluyveromyces lactis

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Phosphoenolpyruvate carboxykinase [ATP]		PRO_0000203873
Regions
	Nucleotide binding	244 – 251	8	ATP Potential
Experimental info
	Sequence conflict	236	1	D → G in AAC27661. Ref.1
	Sequence conflict	255	1	A → G in AAC27661. Ref.1
	Sequence conflict	500	1	S → I in AAC27661. Ref.1
	Sequence conflict	509	1	K → N in AAC27661. Ref.1
	Sequence conflict	520	1	L → W in AAC27661. Ref.1
	Sequence conflict	523	1	E → K in AAC27661. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O43112 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: 35CD60147227B2A4	FASTA	543	60,429
	10 20 30 40 50 60 MSPTKTQNSA EENIRAELGL SAEVVTIRRN APAALLYEDA LKERDTAISN AGALIAYSGD 70 80 90 100 110 120 KTGRSPRDKR IVEEETSKDN VWWGPVNKPC SERTWEINRE RAADYLRTRD HIYIVDAYAG 130 140 150 160 170 180 WDPRYRIKVR VVCARAYHAL FMTNMLIRPS KEELENFGEP DFTVWNAGQF PANTHTSGMT 190 200 210 220 230 240 SKTTVEINFK QMEMVILGTE YAGEMKKGIF TVMFYLMPVN HNVLTLHSSA NQGIQDNDVT 250 260 270 280 290 300 LFFGLSGTGK TTLSADPHRL LIGDDEHCWS DHGVFNIEGG CYAKCLGLSA EKEPEIFNAI 310 320 330 340 350 360 KFGSVLENII YDPNTREVDY EDSTITENTR CAYPIEYIPS AKIPCLADHH PKNIVLLTCD 370 380 390 400 410 420 ASGVLPPVSK LTPDQVMYHF ISGYTSKMAG TEQGVTEPEA TFSSCFGQPF LSLHPMKYAT 430 440 450 460 470 480 MLAEKMAEHN ANAWLINTGW TGSSYVAGGK RCPLKYTRAI LDAIHDGSLA KEEYEVLPIF 490 500 510 520 530 540 NLQIPKAVGD KVPASLLNPS KNWAEGEAKY TSNVKSLANL FVENFKTYQD KATEQVLAAG PQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase from Kluyveromyces lactis." Kitamoto H.K., Ohmomo S., Iimura Y. Yeast 14:963-967(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U88575 Genomic DNA. Translation: AAC27661.1. CR382121 Genomic DNA. Translation: CAH02607.1.
RefSeq	XP_451019.1. XM_451019.1.
3D structure databases
ProteinModelPortal	O43112.
SMR	O43112. Positions 26-543.
ModBase	Search...
Protein-protein interaction databases
STRING	28985.O43112.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2896341.
KEGG	kla:KLLA0A00484g.
Phylogenomic databases
eggNOG	COG1866.
HOGENOM	HOG000271471.
KO	K01610.
OMA	TRCAYPI.
OrthoDB	EOG44N217.
Enzyme and pathway databases
UniPathway	UPA00138.
Family and domain databases
Gene3D	3.40.449.10. 1 hit. 3.90.228.20. 2 hits.
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. pckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_KLULA

Accession

Primary (citable) accession number: O43112
Secondary accession number(s): Q6CYH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	September 27, 2004
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program