Reviewed, UniProtKB/Swiss-Prot O43090 (NDH2_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable NADH-ubiquinone oxidoreductase C947.15c, mitochondrial
    EC=1.6.5.3

Gene names

ORF Names:

SPBC947.15c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidation of NADH By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol.
Subcellular location	Mitochondrion. Ref.2
Sequence similarities	Belongs to the NADH dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion Potential
	Chain	36 – 551	516	Probable NADH-ubiquinone oxidoreductase C947.15c, mitochondrial		PRO_0000337258
Regions
	Nucleotide binding	92 – 122	31	FAD By similarity
	Nucleotide binding	255 – 291	37	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O43090-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: C8CE3C56F88108AC	FASTA	551	60,781
	10 20 30 40 50 60 MSVSKARLQS VVRLSRTVPY SKTMVRSFHV SCAVKNSGNV PTPRNKSFFS RALEMAEVTS 70 80 90 100 110 120 SLSMLGAVAL FQSLRRLNNS SPKGKSGVPK KNIVVLGSGW GAVAAIKNLD PSLYNITLVS 130 140 150 160 170 180 PRDHFLFTPM LPSCTVGTLR LPSITEPIVA LFKGKIDPSN IHQAECTAID TSAKKVTIRG 190 200 210 220 230 240 TTEANEGKEA VIPYDTLVFA IGAGNQTFGI QGVRDHGCFL KEAGDAKKVF NRIFEILEQV 250 260 270 280 290 300 RFNKDLSPEE RARLLHITVV GGGPTGMEFA AEMQDFIDND VKDMFPELQK DIHVTLIEAA 310 320 330 340 350 360 PGVLPMFTKS LITYTENLFK NLNIKIMTKT VVKDVNEKNL IVQKTNPDGS KAMQEIPYGM 370 380 390 400 410 420 LVWAAGITAR PLTRTLMSSI PEQSGARKGL IVDEFFRVKG VPEMYAVGDC AFSGLPATAQ 430 440 450 460 470 480 VANQQGAWLA KNLNVEGKKF ALHERIQALE KQLGEKEAPS QVAGLKQQVE QLKLEPFKYH 490 500 510 520 530 540 HQGALAYVGD EKAIADLKLP FMKKMLPLQG IVGHTFWRLA YLNELISARS QFMVLIDWLK 550 TRLFGRYDAK V

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA17043.1.
PIR	T40767.
RefSeq	NP_595261.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2540211.
KEGG	spo:SPBC947.15c.
Organism-specific databases
GeneDB_Spombe	SPBC947.15c.
Phylogenomic databases
OMA	O43090. DYLIMAY.
Gene expression databases
ArrayExpress	O43090.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProtoNet	Search...

Entry information

Entry name

NDH2_SCHPO

Accession

Primary (citable) accession number: O43090

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)