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Protein

Eukaryotic translation initiation factor 3 subunit F

Gene

eif3f

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. Essential for viability.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translational initiation Source: PomBase
  • formation of cytoplasmic translation initiation complex Source: PomBase
  • formation of translation preinitiation complex Source: UniProtKB-HAMAP
  • proteolysis Source: GOC
  • regulation of translational initiation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72689. Formation of a pool of free 40S subunits.
R-SPO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SPO-72702. Ribosomal scanning and start codon recognition.

Protein family/group databases

MEROPSiM67.975.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit F1 PublicationUniRule annotation
Short name:
eIF3f1 PublicationUniRule annotation
Gene namesi
Name:eif3f1 Publication
ORF Names:SPBC4C3.07
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC4C3.07.
PomBaseiSPBC4C3.07.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • eukaryotic 43S preinitiation complex Source: PomBase
  • eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
  • eukaryotic translation initiation factor 3 complex Source: PomBase
  • eukaryotic translation initiation factor 3 complex, eIF3e Source: PomBase
  • eukaryotic translation initiation factor 3 complex, eIF3m Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Eukaryotic translation initiation factor 3 subunit FPRO_0000347327Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei162 – 1621PhosphoserineUniRule annotation1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO43060.

PTM databases

iPTMnetiO43060.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex appears to include tif32/eif3a, SPAC25G10.08/eif3b, tif33/eif3c, SPBC4C3.07/eif3f, tif35/eif3g and sum1/eif3i. This set of common subunits may also associate exclusively with either moe1/eif3d and int6/eif3e, or with SPAC821.05/eif3h and SPAC1751.03/eif3m. The eIF-3 complex may also include SPAC3A12.13c/eif3j.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi277385. 2 interactions.
MINTiMINT-4674986.

Structurei

3D structure databases

ProteinModelPortaliO43060.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 129112MPNUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-3 subunit F family.UniRule annotation
Contains 1 MPN (JAB/Mov34) domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000241154.
InParanoidiO43060.
KOiK03249.
OMAiDAYERRN.
OrthoDBiEOG7P2Z3Q.
PhylomeDBiO43060.

Family and domain databases

HAMAPiMF_03005. eIF3f.
InterProiIPR027531. eIF3f.
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43060-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALGTKHVLH LTKPSSRSSP LNIVIEPAVL FSILDHSTRK SENNQRVIGT
60 70 80 90 100
LLGTRSEDGR EIEIKSCFAV PHNESSEQVE VEMEYHRAMY HLHLKANPRE
110 120 130 140 150
VVVGWYATSP DLDAFSALIQ NLYASPAEPG TAPLGTYPHP CVHLTVNTDV
160 170 180 190 200
SSPLAIKTYV SSPVGITERL ADSCAFVPTP FTIRDDEAVR SGLKAVAAPK
210 220 230 240 250
NDPSRLASLF TDLQQLRRST LELLSMIERV SDYVQNVIDG SSPANVAVGR
260 270 280 290 300
YLMKCFSLIP CVEGQDFEKI FSSHLQDVLV VVYLANTLRT QVDIASRLNL

LP
Length:302
Mass (Da):33,251
Last modified:June 1, 1998 - v1
Checksum:iA046E087CF083D84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA16829.1.
PIRiT40490.
RefSeqiNP_596298.1. NM_001022219.2.

Genome annotation databases

EnsemblFungiiSPBC4C3.07.1; SPBC4C3.07.1:pep; SPBC4C3.07.
GeneIDi2540868.
KEGGispo:SPBC4C3.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA16829.1.
PIRiT40490.
RefSeqiNP_596298.1. NM_001022219.2.

3D structure databases

ProteinModelPortaliO43060.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277385. 2 interactions.
MINTiMINT-4674986.

Protein family/group databases

MEROPSiM67.975.

PTM databases

iPTMnetiO43060.

Proteomic databases

MaxQBiO43060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC4C3.07.1; SPBC4C3.07.1:pep; SPBC4C3.07.
GeneIDi2540868.
KEGGispo:SPBC4C3.07.

Organism-specific databases

EuPathDBiFungiDB:SPBC4C3.07.
PomBaseiSPBC4C3.07.

Phylogenomic databases

HOGENOMiHOG000241154.
InParanoidiO43060.
KOiK03249.
OMAiDAYERRN.
OrthoDBiEOG7P2Z3Q.
PhylomeDBiO43060.

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72689. Formation of a pool of free 40S subunits.
R-SPO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SPO-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

PROiO43060.

Family and domain databases

HAMAPiMF_03005. eIF3f.
InterProiIPR027531. eIF3f.
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "PCI proteins eIF3e and eIF3m define distinct translation initiation factor 3 complexes."
    Zhou C., Arslan F., Wee S., Krishnan S., Ivanov A.R., Oliva A., Leatherwood J., Wolf D.A.
    BMC Biol. 3:14-14(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE EIF-3 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiEIF3F_SCHPO
AccessioniPrimary (citable) accession number: O43060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 1, 1998
Last modified: June 8, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.