O43056 (KHSE_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

ORF Names:

SPBC4C3.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine.
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine metabolic process Inferred from sequence or structural similarity. Source: PomBase threonine biosynthetic process Inferred from sequence or structural similarity. Source: PomBase
Cellular_component	cytoplasm Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from sequence or structural similarity. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Probable homoserine kinase		PRO_0000156655
Regions
	Nucleotide binding	93 – 103	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O43056 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 17B4F94188F6267B	FASTA	338	36,608
	10 20 30 40 50 60 MQKFQIKVPA SSANIGPGFD VLGMSLEEYM TLDVEVSTES GPCVLTYEGD GKEHVSLDVQ 70 80 90 100 110 120 KNMITQTSLY VLRCNNISTF PYATKIHVIN PIPLGRGMGS SGSAAIAGVM LANEIAKLGL 130 140 150 160 170 180 SKLQMMDYVL MIERHPDNVM ASMMGGFVGS FLRELSEEEK NAFSPSADDL LKNEALTLPP 190 200 210 220 230 240 KSLGTFARLP WASELKAIVV IPEFHLATSK ARSVLPTSYG RTDVVYNLQR LALLTTALGQ 250 260 270 280 290 300 TPINPHLVYE VMKDKVHQPY RASLIPGLQN ILATLNPDTQ PGLCGICLSG AGPTVLALAT 310 320 330 GNFDEIAHAM LSIFEKHGVK CRYLVLSPAF DGATVKYF

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA16824.1.
PIR	T40495.
RefSeq	NP_596303.1. NM_001022224.2.
3D structure databases
ProteinModelPortal	O43056.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC4C3.03-1.
Proteomic databases
PaxDb	O43056.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC4C3.03.1; SPBC4C3.03.1:pep; SPBC4C3.03.
GeneID	2540857.
KEGG	spo:SPBC4C3.03.
Organism-specific databases
PomBase	SPBC4C3.03.
Phylogenomic databases
eggNOG	COG0083.
HOGENOM	HOG000247199.
KO	K00872.
OMA	LHEPYRW.
OrthoDB	EOG4B8NNT.
Enzyme and pathway databases
UniPathway	UPA00050; UER00064.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
InterPro	IPR006204. GHMP_kinase_N_dom. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801973.

Entry information

Entry name

KHSE_SCHPO

Accession

Primary (citable) accession number: O43056

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program