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Protein

Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

alg6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc(2)-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: PomBase

GO - Biological processi

  • oligosaccharide-lipid intermediate biosynthetic process Source: PomBase
  • protein N-linked glycosylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)
Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:alg6
ORF Names:SPBC342.01c, SPBC3F6.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC342.01c.
PomBaseiSPBC342.01c. alg6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Transmembranei343 – 363HelicalSequence analysisAdd BLAST21
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Transmembranei465 – 485HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: PomBase
  • endoplasmic reticulum membrane Source: GO_Central
  • fungal-type vacuole membrane Source: PomBase
  • Golgi apparatus Source: PomBase
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741601 – 506Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST506

Proteomic databases

PRIDEiO43053.

Interactioni

Protein-protein interaction databases

BioGridi277509. 49 interactors.
MINTiMINT-4674956.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000195048.
InParanoidiO43053.
KOiK03848.
OMAiTTMAFFI.
OrthoDBiEOG092C0OC7.
PhylomeDBiO43053.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSEFENGAP VQQFVSRFRS YSSKFLFFPC LIMSLVFMQW LISIGPYSGY
60 70 80 90 100
NTPPMYGDFE AQRHWMELTL HTPVSQWYFR DLQWWGLDYP PLTAYVSWFF
110 120 130 140 150
GIIGHYFFNP EWFADVTSRG FESLELKLFM RSTVIASHLL ILVPPLMFYS
160 170 180 190 200
KWWSRRIPNF VDRNASLIMV LFQPALLLID HGHFQYNCVM LGLVMYAIAN
210 220 230 240 250
LLKNQYVAAT FFFCLALTFK QMALYFAPPI FFYLLGTCVK PKIRFSRFIL
260 270 280 290 300
LSVTVVFTFS LILFPWIYMD YKTLLPQILH RVFPFARGLW EDKVANFWCT
310 320 330 340 350
LNTVFKIREV FTLHQLQVIS LIFTLISILP SCVILFLYPR KRLLALGFAS
360 370 380 390 400
ASWGFFLFSF QVHEKSVLLP LLPTSILLCH GNITTKPWIA LANNLAVFSL
410 420 430 440 450
WPLLKKDGLG LQYFTLVLMW NWIGDMVVFS KNVLFRFIQL SFYVGMIVIL
460 470 480 490 500
GIDLFIPPPS RYPDLWVILN VTLSFAGFFT IYLWTLGRLL HISSKLSTDL

RNKKEA
Length:506
Mass (Da):59,203
Last modified:June 1, 1998 - v1
Checksum:i0CD8ACC78BF49D6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17695.1.
PIRiT40396.
RefSeqiNP_596744.1. NM_001023764.2.

Genome annotation databases

EnsemblFungiiSPBC342.01c.1; SPBC342.01c.1:pep; SPBC342.01c.
GeneIDi2540993.
KEGGispo:SPBC342.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17695.1.
PIRiT40396.
RefSeqiNP_596744.1. NM_001023764.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277509. 49 interactors.
MINTiMINT-4674956.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Proteomic databases

PRIDEiO43053.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC342.01c.1; SPBC342.01c.1:pep; SPBC342.01c.
GeneIDi2540993.
KEGGispo:SPBC342.01c.

Organism-specific databases

EuPathDBiFungiDB:SPBC342.01c.
PomBaseiSPBC342.01c. alg6.

Phylogenomic databases

HOGENOMiHOG000195048.
InParanoidiO43053.
KOiK03848.
OMAiTTMAFFI.
OrthoDBiEOG092C0OC7.
PhylomeDBiO43053.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiO43053.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 1 hit.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG6_SCHPO
AccessioniPrimary (citable) accession number: O43053
Secondary accession number(s): Q9UUC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.