O43053 (ALG6_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
EC=2.4.1.267

Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase

Gene names

Name:	alg6
ORF Names:	SPBC342.01c, SPBC3F6.06c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Dolichyl beta-D-glucosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ALG6/ALG8 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	oligosaccharide-lipid intermediate biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	Golgi apparatus Inferred from direct assay. Source: GeneDB_Spombe endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type vacuole membrane Inferred from direct assay. Source: GeneDB_Spombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase		PRO_0000174160
Regions
	Transmembrane	25 – 45	21	Helical; Potential
	Transmembrane	133 – 153	21	Helical; Potential
	Transmembrane	189 – 209	21	Helical; Potential
	Transmembrane	212 – 232	21	Helical; Potential
	Transmembrane	248 – 268	21	Helical; Potential
	Transmembrane	318 – 338	21	Helical; Potential
	Transmembrane	343 – 363	21	Helical; Potential
	Transmembrane	383 – 403	21	Helical; Potential
	Transmembrane	437 – 457	21	Helical; Potential
	Transmembrane	465 – 485	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O43053 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 0CD8ACC78BF49D6B	FASTA	506	59,203
	10 20 30 40 50 60 MLSEFENGAP VQQFVSRFRS YSSKFLFFPC LIMSLVFMQW LISIGPYSGY NTPPMYGDFE 70 80 90 100 110 120 AQRHWMELTL HTPVSQWYFR DLQWWGLDYP PLTAYVSWFF GIIGHYFFNP EWFADVTSRG 130 140 150 160 170 180 FESLELKLFM RSTVIASHLL ILVPPLMFYS KWWSRRIPNF VDRNASLIMV LFQPALLLID 190 200 210 220 230 240 HGHFQYNCVM LGLVMYAIAN LLKNQYVAAT FFFCLALTFK QMALYFAPPI FFYLLGTCVK 250 260 270 280 290 300 PKIRFSRFIL LSVTVVFTFS LILFPWIYMD YKTLLPQILH RVFPFARGLW EDKVANFWCT 310 320 330 340 350 360 LNTVFKIREV FTLHQLQVIS LIFTLISILP SCVILFLYPR KRLLALGFAS ASWGFFLFSF 370 380 390 400 410 420 QVHEKSVLLP LLPTSILLCH GNITTKPWIA LANNLAVFSL WPLLKKDGLG LQYFTLVLMW 430 440 450 460 470 480 NWIGDMVVFS KNVLFRFIQL SFYVGMIVIL GIDLFIPPPS RYPDLWVILN VTLSFAGFFT 490 500 IYLWTLGRLL HISSKLSTDL RNKKEA

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA17695.1.
PIR	T40396.
RefSeq	NP_596744.1. NM_001023764.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O43053.
Protein family/group databases
CAZy	GT57. Glycosyltransferase Family 57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC342.01c.1; SPBC342.01c.1:pep; SPBC342.01c.
GeneID	2540993.
GenomeReviews	Gene locus alg6 in contig CU329671_GR.
KEGG	spo:SPBC342.01c.
NMPDR	fig|4896.1.peg.2610.
Organism-specific databases
GeneDB_Spombe	SPBC342.01c.
Phylogenomic databases
eggNOG	fuNOG06801.
GeneTree	EFGT00050000004500.
HOGENOM	HBG621987.
OMA	ALYYAPV.
OrthoDB	EOG4936SH.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004905-MONOMER.
Gene expression databases
ArrayExpress	O43053.
Family and domain databases
InterPro	IPR004856. Glyco_trans_ALG6/ALG8. [Graphical view]
KO	K03848.
PANTHER	PTHR12413. Alg6_Alg8. 1 hit.
Pfam	PF03155. Alg6_Alg8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG6_SCHPO

Accession

Primary (citable) accession number: O43053
Secondary accession number(s): Q9UUC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	June 1, 1998
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program