Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

alg6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc2-PP-Dol (By similarity).By similarity

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: PomBase

GO - Biological processi

  • dolichol-linked oligosaccharide biosynthetic process Source: PomBase
  • oligosaccharide-lipid intermediate biosynthetic process Source: PomBase
  • protein N-linked glycosylation Source: PomBase
  • protein N-linked glycosylation via asparagine Source: PomBase

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT57 Glycosyltransferase Family 57

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)
Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:alg6
ORF Names:SPBC342.01c, SPBC3F6.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC342.01c
PomBaseiSPBC342.01c alg6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Transmembranei343 – 363HelicalSequence analysisAdd BLAST21
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Transmembranei465 – 485HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741601 – 506Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST506

Proteomic databases

PaxDbiO43053
PRIDEiO43053

Interactioni

Protein-protein interaction databases

BioGridi277509, 49 interactors
STRINGi4896.SPBC342.01c.1

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000195048
InParanoidiO43053
KOiK03848
OMAiFINPDWI
OrthoDBiEOG092C0OC7
PhylomeDBiO43053

Family and domain databases

InterProiView protein in InterPro
IPR004856 Glyco_trans_ALG6/ALG8
PANTHERiPTHR12413 PTHR12413, 1 hit
PfamiView protein in Pfam
PF03155 Alg6_Alg8, 1 hit

Sequencei

Sequence statusi: Complete.

O43053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSEFENGAP VQQFVSRFRS YSSKFLFFPC LIMSLVFMQW LISIGPYSGY
60 70 80 90 100
NTPPMYGDFE AQRHWMELTL HTPVSQWYFR DLQWWGLDYP PLTAYVSWFF
110 120 130 140 150
GIIGHYFFNP EWFADVTSRG FESLELKLFM RSTVIASHLL ILVPPLMFYS
160 170 180 190 200
KWWSRRIPNF VDRNASLIMV LFQPALLLID HGHFQYNCVM LGLVMYAIAN
210 220 230 240 250
LLKNQYVAAT FFFCLALTFK QMALYFAPPI FFYLLGTCVK PKIRFSRFIL
260 270 280 290 300
LSVTVVFTFS LILFPWIYMD YKTLLPQILH RVFPFARGLW EDKVANFWCT
310 320 330 340 350
LNTVFKIREV FTLHQLQVIS LIFTLISILP SCVILFLYPR KRLLALGFAS
360 370 380 390 400
ASWGFFLFSF QVHEKSVLLP LLPTSILLCH GNITTKPWIA LANNLAVFSL
410 420 430 440 450
WPLLKKDGLG LQYFTLVLMW NWIGDMVVFS KNVLFRFIQL SFYVGMIVIL
460 470 480 490 500
GIDLFIPPPS RYPDLWVILN VTLSFAGFFT IYLWTLGRLL HISSKLSTDL

RNKKEA
Length:506
Mass (Da):59,203
Last modified:June 1, 1998 - v1
Checksum:i0CD8ACC78BF49D6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA17695.1
PIRiT40396
RefSeqiNP_596744.1, NM_001023764.2

Genome annotation databases

EnsemblFungiiSPBC342.01c.1; SPBC342.01c.1:pep; SPBC342.01c
GeneIDi2540993
KEGGispo:SPBC342.01c

Similar proteinsi

Entry informationi

Entry nameiALG6_SCHPO
AccessioniPrimary (citable) accession number: O43053
Secondary accession number(s): Q9UUC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health