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Protein

L-pipecolate oxidase

Gene

fap1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes L-pipecolate and L-proline (6,7% of the activity for L-pipecolate).1 Publication

Catalytic activityi

L-pipecolate + O2 = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2.1 Publication

Cofactori

FAD1 Publication

Kineticsi

  1. KM=4.07 mM for L-pipecolate1 Publication
  2. KM=33.5 mM for L-proline1 Publication
  1. Vmax=46.1 µmol/min/mg enzyme for L-pipecolate1 Publication
  2. Vmax=9.45 µmol/min/mg enzyme for L-proline1 Publication

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: PomBase
  • L-pipecolate oxidase activity Source: PomBase
  • proline oxidase activity Source: PomBase

GO - Biological processi

  • L-lysine catabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

SABIO-RKO43029.

Names & Taxonomyi

Protein namesi
Recommended name:
L-pipecolate oxidaseImported (EC:1.5.3.7)
Alternative name(s):
L-pipecolic acid oxidase1 Publication
Gene namesi
Name:fap1Imported
ORF Names:SPBC354.15
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC354.15.
PomBaseiSPBC354.15. fap1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • extracellular region Source: UniProtKB-SubCell
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 412392L-pipecolate oxidaseSequence analysisPRO_0000347274Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO43029.

Interactioni

Subunit structurei

Dimer.1 Publication

Protein-protein interaction databases

BioGridi277432. 6 interactions.
MINTiMINT-4674736.

Structurei

3D structure databases

ProteinModelPortaliO43029.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MSOX/MTOX family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000169862.
KOiK00306.
OMAiVEALEVW.
PhylomeDBiO43029.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43029-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKNTSVIIV GAGVFGLSAA LELTKRGGYT IKILDRAPPP VIDGSSVDAN
60 70 80 90 100
RIIRSDYADA VYCSMGIDAL EEWRTNPLFK EQFYGSGLMF VGRDNVEYRD
110 120 130 140 150
MSLENLTKMG VSAAKFQTTE ELRKLFPKWI GELNDGEAGY ANFSSGWANA
160 170 180 190 200
EQSVKSVVNY LAHAGVSFIS GPEGTVEELI TEENVVKGVR TTTGAYMAEK
210 220 230 240 250
LIFATGAWTA SLLPNDHTRF LATGQPVAYI KLTPEEYIRF LTNPVYLDFD
260 270 280 290 300
TGFYIFPPTP DGYLKFARHG YGFTRMQNLK SGKVESVPPK KPLVSPILPK
310 320 330 340 350
EAELDLRRNL QRTYGEEISQ RPFYKTRICY YTDTADAEFV FDYHPDYENL
360 370 380 390 400
FVCTGGSGHG FKFFPILGKY SIGCMFRELE EPLLKKWRWK KENLEFAALD
410
HSRAGPSRQE LS
Length:412
Mass (Da):46,320
Last modified:June 1, 1998 - v1
Checksum:iBD36B86075D3F4EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17815.1.
PIRiT40295.
RefSeqiNP_595239.1. NM_001021145.2.

Genome annotation databases

EnsemblFungiiSPBC354.15.1; SPBC354.15.1:pep; SPBC354.15.
GeneIDi2540916.
KEGGispo:SPBC354.15.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17815.1.
PIRiT40295.
RefSeqiNP_595239.1. NM_001021145.2.

3D structure databases

ProteinModelPortaliO43029.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277432. 6 interactions.
MINTiMINT-4674736.

Proteomic databases

MaxQBiO43029.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC354.15.1; SPBC354.15.1:pep; SPBC354.15.
GeneIDi2540916.
KEGGispo:SPBC354.15.

Organism-specific databases

EuPathDBiFungiDB:SPBC354.15.
PomBaseiSPBC354.15. fap1.

Phylogenomic databases

HOGENOMiHOG000169862.
KOiK00306.
OMAiVEALEVW.
PhylomeDBiO43029.

Enzyme and pathway databases

SABIO-RKO43029.

Miscellaneous databases

PROiO43029.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Characterization of two fructosyl-amino acid oxidase homologs of Schizosaccharomyces pombe."
    Yoshida N., Akazawa S., Katsuragi T., Tani Y.
    J. Biosci. Bioeng. 97:278-280(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFAP1_SCHPO
AccessioniPrimary (citable) accession number: O43029
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 1, 1998
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.