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Protein

Glyceraldehyde-3-phosphate dehydrogenase 2

Gene

gpd3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd3), Glyceraldehyde-3-phosphate dehydrogenase 1 (tdh1)
  2. Phosphoglycerate kinase (pgk1)
  3. Phosphoglycerate mutase (gpm1)
  4. Enolase 1-2 (eno102), Enolase 1-1 (eno101)
  5. Pyruvate kinase (pyk1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADBy similarity1
Binding sitei80NAD; via carbonyl oxygenBy similarity1
Active sitei152NucleophilePROSITE-ProRule annotation1
Sitei179Activates thiol group during catalysisBy similarity1
Binding sitei182Glyceraldehyde 3-phosphateBy similarity1
Binding sitei234Glyceraldehyde 3-phosphateBy similarity1
Binding sitei316NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 14NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD

Enzyme and pathway databases

ReactomeiR-SPO-70171 Glycolysis
R-SPO-70263 Gluconeogenesis
UniPathwayiUPA00109; UER00184

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 2 (EC:1.2.1.12)
Short name:
GAPDH 2
Gene namesi
Name:gpd3
ORF Names:SPBC354.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC354.12
PomBaseiSPBC354.12 gpd3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001455811 – 335Glyceraldehyde-3-phosphate dehydrogenase 2Add BLAST335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei125Phosphoserine1 Publication1
Modified residuei151Phosphoserine1 Publication1
Modified residuei153Phosphothreonine1 Publication1
Modified residuei154Phosphothreonine1 Publication1
Modified residuei182Phosphothreonine1 Publication1
Modified residuei184Phosphothreonine1 Publication1
Modified residuei192Phosphoserine1 Publication1
Modified residuei203Phosphoserine1 Publication1
Modified residuei209Phosphoserine1 Publication1
Modified residuei211Phosphothreonine1 Publication1
Modified residuei237Phosphothreonine1 Publication1
Modified residuei241Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO43026
PaxDbiO43026
PRIDEiO43026

PTM databases

iPTMnetiO43026

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi277491, 65 interactors
MINTiO43026
STRINGi4896.SPBC354.12.1

Structurei

3D structure databases

ProteinModelPortaliO43026
SMRiO43026
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 153Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni211 – 212Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000071678
InParanoidiO43026
KOiK00134
OMAiFTLENMV
OrthoDBiEOG092C2MZH
PhylomeDBiO43026

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

O43026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPKVGING FGRIGRIVLR NAILTGKIQV VAVNDPFIDL DYMAYMFKYD
60 70 80 90 100
STHGRFEGSV ETKGGKLVID GHSIDVHNER DPANIKWSAS GAEYVIESTG
110 120 130 140 150
VFTTKETASA HLKGGAKRVI ISAPSKDAPM FVVGVNLEKF NPSEKVISNA
160 170 180 190 200
SCTTNCLAPL AKVINDTFGI EEGLMTTVHA TTATQKTVDG PSKKDWRGGR
210 220 230 240 250
GASANIIPSS TGAAKAVGKV IPALNGKLTG MAFRVPTPDV SVVDLTVKLA
260 270 280 290 300
KPTNYEDIKA AIKAASEGPM KGVLGYTEDS VVSTDFCGDN HSSIFDASAG
310 320 330
IQLSPQFVKL VSWYDNEWGY SHRVVDLVAY TASKD
Length:335
Mass (Da):35,675
Last modified:June 1, 1998 - v1
Checksum:iF0A0FB6AED5EB625
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA17812.1
PIRiT40292
RefSeqiNP_595236.1, NM_001021142.2

Genome annotation databases

EnsemblFungiiSPBC354.12.1; SPBC354.12.1:pep; SPBC354.12
KEGGispo:SPBC354.12

Similar proteinsi

Entry informationi

Entry nameiG3P2_SCHPO
AccessioniPrimary (citable) accession number: O43026
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health