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Protein

Mannose-6-phosphate isomerase

Gene

pmi40

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (pmi40)
  2. Phosphomannomutase (pmm1)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99ZincBy similarity1
Metal bindingi101ZincBy similarity1
Metal bindingi126ZincBy similarity1
Metal bindingi265ZincBy similarity1
Active sitei284By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-446205. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:pmi40
ORF Names:SPBC2G2.16
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC2G2.16.
PomBaseiSPBC2G2.16.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003147741 – 412Mannose-6-phosphate isomeraseAdd BLAST412

Proteomic databases

MaxQBiO43014.
PRIDEiO43014.

Interactioni

Protein-protein interaction databases

MINTiMINT-4674647.

Structurei

3D structure databases

ProteinModelPortaliO43014.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000241277.
InParanoidiO43014.
KOiK01809.
OMAiQGEPWDT.
OrthoDBiEOG092C3NWF.
PhylomeDBiO43014.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRLKCDVKS YGWGKVGHES LAAKLAEAGY GFEVDPNTPY AELWMGSHPS
60 70 80 90 100
GPSFVMQTGK PLSELLTPET VGEKVYKKYG KQLPFLFKVL SINKVLSIQA
110 120 130 140 150
HPDKPLGKQL HKTNPKEYKD DNHKPEMAVA LTEFDALCGF RPVKQIEQFL
160 170 180 190 200
DSIAPLREFV GEEAVRQFKG TVKQDERKAL ETLFNELMHK DEKRIQEFTP
210 220 230 240 250
QLVQLAKSDA NNFGGTEYGG KAFSDLIIHL NSQFPDDIGL FVTPFLNYVR
260 270 280 290 300
LHPGEAVFLR ALDPHAYVSG NIIECMAASD NVIRLGFTPK FKDIETLVNN
310 320 330 340 350
LTYQTADAKS QLTQPVPFAK ACGSGKTLLY DPPIEEFSIL QTKVSPGQKQ
360 370 380 390 400
CIRGINGPSI LLVTEGSGIL NGDKGDVASI SPGFVYFISA NFPLTISATS
410
EPVVVYQAFC EI
Length:412
Mass (Da):45,590
Last modified:June 1, 1998 - v1
Checksum:iE1B4685C02B25C89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17896.1.
PIRiT40155.
RefSeqiNP_596445.1. NM_001022364.2.

Genome annotation databases

EnsemblFungiiSPBC2G2.16.1; SPBC2G2.16.1:pep; SPBC2G2.16.
GeneIDi2540428.
KEGGispo:SPBC2G2.16.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17896.1.
PIRiT40155.
RefSeqiNP_596445.1. NM_001022364.2.

3D structure databases

ProteinModelPortaliO43014.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4674647.

Proteomic databases

MaxQBiO43014.
PRIDEiO43014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC2G2.16.1; SPBC2G2.16.1:pep; SPBC2G2.16.
GeneIDi2540428.
KEGGispo:SPBC2G2.16.

Organism-specific databases

EuPathDBiFungiDB:SPBC2G2.16.
PomBaseiSPBC2G2.16.

Phylogenomic databases

HOGENOMiHOG000241277.
InParanoidiO43014.
KOiK01809.
OMAiQGEPWDT.
OrthoDBiEOG092C3NWF.
PhylomeDBiO43014.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.
ReactomeiR-SPO-446205. Synthesis of GDP-mannose.

Miscellaneous databases

PROiO43014.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPI_SCHPO
AccessioniPrimary (citable) accession number: O43014
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.