O43014 (MPI_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-6-phosphate isomerase
EC=5.3.1.8

Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI

Gene names

Name:	pmi40
ORF Names:	SPBC2G2.16

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm. Nucleus Ref.2.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GDP-mannose biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe cell wall mannoprotein biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe protein glycosylation Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	mannose-6-phosphate isomerase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Mannose-6-phosphate isomerase		PRO_0000314774
Sites
	Active site	284	1	By similarity
	Metal binding	99	1	Zinc By similarity
	Metal binding	101	1	Zinc By similarity
	Metal binding	126	1	Zinc By similarity
	Metal binding	265	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	O43014 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: E1B4685C02B25C89	FASTA	412	45,590
	10 20 30 40 50 60 MYRLKCDVKS YGWGKVGHES LAAKLAEAGY GFEVDPNTPY AELWMGSHPS GPSFVMQTGK 70 80 90 100 110 120 PLSELLTPET VGEKVYKKYG KQLPFLFKVL SINKVLSIQA HPDKPLGKQL HKTNPKEYKD 130 140 150 160 170 180 DNHKPEMAVA LTEFDALCGF RPVKQIEQFL DSIAPLREFV GEEAVRQFKG TVKQDERKAL 190 200 210 220 230 240 ETLFNELMHK DEKRIQEFTP QLVQLAKSDA NNFGGTEYGG KAFSDLIIHL NSQFPDDIGL 250 260 270 280 290 300 FVTPFLNYVR LHPGEAVFLR ALDPHAYVSG NIIECMAASD NVIRLGFTPK FKDIETLVNN 310 320 330 340 350 360 LTYQTADAKS QLTQPVPFAK ACGSGKTLLY DPPIEEFSIL QTKVSPGQKQ CIRGINGPSI 370 380 390 400 410 LLVTEGSGIL NGDKGDVASI SPGFVYFISA NFPLTISATS EPVVVYQAFC EI

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA17896.1.
PIR	T40155.
RefSeq	NP_596445.1. NM_001022364.1.
3D structure databases
HSSP	HSSP built from PDB template 1PMI based on UniProtKB P34948.
ProteinModelPortal	O43014.
ModBase	Search...
Protein-protein interaction databases
STRING	O43014.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC2G2.16.1; SPBC2G2.16.1:pep; SPBC2G2.16.
GeneID	2540428.
GenomeReviews	Gene locus pmi40 in contig CU329671_GR.
KEGG	spo:SPBC2G2.16.
NMPDR	fig|4896.1.peg.2311.
Organism-specific databases
GeneDB_Spombe	SPBC2G2.16.
Phylogenomic databases
eggNOG	fuNOG04716.
GeneTree	EFGT00050000001229.
HOGENOM	HBG590632.
OMA	YGIANPD.
OrthoDB	EOG4TF3V5.
Gene expression databases
ArrayExpress	O43014.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 3 hits.
KO	K01809.
PANTHER	PTHR10309. PTHR10309. 1 hit.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR00218. ManA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPI_SCHPO

Accession

Primary (citable) accession number: O43014

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 1998
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program