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Protein

Histidine--tRNA ligase, mitochondrial

Gene

hts1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aminoacylation of histidyl-tRNA in both the cytoplasm and the mitochondrion.By similarity

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine--tRNA ligase, mitochondrial (EC:6.1.1.21)
Alternative name(s):
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:hts1
ORF Names:SPBC2G2.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC2G2.12.
PomBaseiSPBC2G2.12.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25MitochondrionSequence analysisAdd BLAST25
ChainiPRO_000031475526 – 563Histidine--tRNA ligase, mitochondrialAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO43011.
PRIDEiO43011.

PTM databases

iPTMnetiO43011.

Interactioni

Protein-protein interaction databases

BioGridi277000. 6 interactors.
MINTiMINT-4674620.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 85WHEP-TRSPROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Contains 1 WHEP-TRS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000018075.
InParanoidiO43011.
KOiK01892.
OMAiGWNGFLK.
OrthoDBiEOG092C1ISK.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
IPR009068. S15_NS1_RNA-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVFTKNLFT TKFSSSLLNW RFLRTMADSL VDINERITSQ GNLVRSLKSQ
60 70 80 90 100
GASKEDIDKE VAKLLQLKNL KLGGSEVSGK KKDTSFTLKT PKGTKDWCDK
110 120 130 140 150
DVVLREHIFS TVTEIFKRHG GVTLDTPVFE LKEILSGKYG EDSKLIYDLK
160 170 180 190 200
DQGGELCSLR YDLTVPFARW LAMNPKVTSI KRFHIAKVYR RDQPAMTKGR
210 220 230 240 250
MREFYQCDFD IAGNYDPMIP DSEALKVLVE ALDALEVGNY TIKLNHRKIL
260 270 280 290 300
DGIFTVCGVP DDKIRTISSA VDKLDKLPWE DVRREMVVEK GLKEEVADKI
310 320 330 340 350
KEYVLLKGDR SLLDKLEADS LLSSNSSAVA AFNDMRLLFD YLEAFGVLDR
360 370 380 390 400
FSFDMSLARG LDYYTGIIYE AVTEASAPKI KSSAEKKKSA DPEADRSNDD
410 420 430 440 450
SIGVGSIAAG GRYDNLVGMF AAKKNAKIPC VGISLGLERI FSILRSKIPD
460 470 480 490 500
EDIRANDVDV FVMAFGGGKE WTGFLKERMS VCKDLWANGI KAEFLYKVKP
510 520 530 540 550
KPRQQFDAAD KFDVPFAVIL GQDEFAKGSV RIKQMGLKEN SDEGQLVPRE
560
EMVSVLKKLL KYD
Length:563
Mass (Da):63,305
Last modified:October 3, 2012 - v2
Checksum:iDD0FC33F27AB449B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17892.2.
PIRiT40151.
RefSeqiNP_596441.2. NM_001022360.2.

Genome annotation databases

EnsemblFungiiSPBC2G2.12.1; SPBC2G2.12.1:pep; SPBC2G2.12.
GeneIDi2540472.
KEGGispo:SPBC2G2.12.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17892.2.
PIRiT40151.
RefSeqiNP_596441.2. NM_001022360.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277000. 6 interactors.
MINTiMINT-4674620.

PTM databases

iPTMnetiO43011.

Proteomic databases

MaxQBiO43011.
PRIDEiO43011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC2G2.12.1; SPBC2G2.12.1:pep; SPBC2G2.12.
GeneIDi2540472.
KEGGispo:SPBC2G2.12.

Organism-specific databases

EuPathDBiFungiDB:SPBC2G2.12.
PomBaseiSPBC2G2.12.

Phylogenomic databases

HOGENOMiHOG000018075.
InParanoidiO43011.
KOiK01892.
OMAiGWNGFLK.
OrthoDBiEOG092C1ISK.

Miscellaneous databases

PROiO43011.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
IPR009068. S15_NS1_RNA-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYH_SCHPO
AccessioniPrimary (citable) accession number: O43011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.