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Protein

C-1-tetrahydrofolate synthase, mitochondrial

Gene

ade9

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei227NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi202 – 204NADPBy similarity3
Nucleotide bindingi405 – 412ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-196757. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
C-1-tetrahydrofolate synthase, mitochondrial
Short name:
C1-THF synthase
Including the following 3 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Formyltetrahydrofolate synthetase (EC:6.3.4.3)
Gene namesi
Name:ade9
ORF Names:SPBC2G2.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC2G2.08.
PomBaseiSPBC2G2.08. ade9.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55MitochondrionSequence analysisAdd BLAST55
ChainiPRO_000031562756 – 972C-1-tetrahydrofolate synthase, mitochondrialAdd BLAST917

Proteomic databases

MaxQBiO43007.
PRIDEiO43007.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi276831. 1 interactor.
MINTiMINT-4674578.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 340Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd BLAST285
Regioni84 – 88Substrate bindingBy similarity5
Regioni131 – 133Substrate bindingBy similarity3
Regioni299 – 303Substrate bindingBy similarity5
Regioni341 – 972Formyltetrahydrofolate synthetaseAdd BLAST632

Domaini

This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000040280.
InParanoidiO43007.
KOiK00288.
OrthoDBiEOG092C0B4M.

Family and domain databases

CDDicd00477. FTHFS. 1 hit.
Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 1 hit.
HAMAPiMF_01543. FTHFS. 1 hit.
MF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVMVSFNQL RNYFLESNSL RPSKWLFQSY GTSSSANILN GKLLARKLQR
60 70 80 90 100
SVAEEVQALK AKDRNFKPAL AIVQVGKRED SNVYVRMKEK AARLVGIDFK
110 120 130 140 150
YCPFPETIQM PALLHELKKL NDDHTVHGVL VQLPLPKHLN ERTVTESITP
160 170 180 190 200
PKDVDGFGAF NIGLLAKNDA TPIHYPCTPK GIMELLKDNK ISVAGLNAVV
210 220 230 240 250
LGRSDIVGNP ISYLLRKDNA TVTVCHSKTK DLIQHISNAD LVIAALGKPE
260 270 280 290 300
FVRGEWLKPG SVVVDVGINA VQRNGKRVLV GDVHFESASK VASSITPVPG
310 320 330 340 350
GVGPMTVAML MENIVNAAKI ARTENIYRKI DLNPLELKKP VPSDIEIANS
360 370 380 390 400
QEPKLISNLA KEMGIYDTEL ENYGNYKAKV NLAVYERLKH RKDGNYVVVS
410 420 430 440 450
GITPTPFGEG KSTVVAGLVQ AMGHLGKLGI ACVRQPSQGP TFGVKGGAAG
460 470 480 490 500
GGYAQFIPMD DFNLHMTGDI HAVTAANNLL VAALETRMFH ENTQSDAALI
510 520 530 540 550
KRLIPVKNGR RVIPRGLIGR WNRICASHNM DPEDVNNASP ELLKEFVRLN
560 570 580 590 600
VDPDTIECNR VLDVNDRFLR SIEVGKASTE KGHVRKTSFD ISVASECMSI
610 620 630 640 650
LALSCDLNDM HSRLSRMVIA NDKYGNAITA GDLGVSGALT VLLKDAIKPN
660 670 680 690 700
LMQTLEGTPA FVHAGPFANI SIGASSIIAD KIALKLAGTE SFDRPEDAGY
710 720 730 740 750
VVTEAGFASD MGMEKFFNIK CRYSKLVPNT VVLVTTVKAL KLHGGGPKLK
760 770 780 790 800
PGAPIPEEYL VENLDLVKNG CSNMVKHIQN CHKFNIPVVV AINSYKTDSS
810 820 830 840 850
KEHEIIREAA LQAGAVDAVP SDHWAQGGKG AIELAKSVMT ACDQSSNSKF
860 870 880 890 900
RLLYDSETSI EDKVNVIAKE MYGANGVEFS SLAKERINTF IKQGFGNLPI
910 920 930 940 950
CMAKTQYSLS HNPEFRNVPK NFTVPIRDMR LNAGAGFIYP LAAEIQTIPG
960 970
LPTAPAYLNI DICENGEIVG LS
Length:972
Mass (Da):105,757
Last modified:October 3, 2012 - v3
Checksum:iA14DFE32C0106F20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17888.3.
PIRiT40147.
RefSeqiNP_596437.2. NM_001022356.2.

Genome annotation databases

EnsemblFungiiSPBC2G2.08.1; SPBC2G2.08.1:pep; SPBC2G2.08.
GeneIDi2540300.
KEGGispo:SPBC2G2.08.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17888.3.
PIRiT40147.
RefSeqiNP_596437.2. NM_001022356.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276831. 1 interactor.
MINTiMINT-4674578.

Proteomic databases

MaxQBiO43007.
PRIDEiO43007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC2G2.08.1; SPBC2G2.08.1:pep; SPBC2G2.08.
GeneIDi2540300.
KEGGispo:SPBC2G2.08.

Organism-specific databases

EuPathDBiFungiDB:SPBC2G2.08.
PomBaseiSPBC2G2.08. ade9.

Phylogenomic databases

HOGENOMiHOG000040280.
InParanoidiO43007.
KOiK00288.
OrthoDBiEOG092C0B4M.

Enzyme and pathway databases

UniPathwayiUPA00193.
ReactomeiR-SPO-196757. Metabolism of folate and pterines.

Miscellaneous databases

PROiO43007.

Family and domain databases

CDDicd00477. FTHFS. 1 hit.
Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 1 hit.
HAMAPiMF_01543. FTHFS. 1 hit.
MF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC1TM_SCHPO
AccessioniPrimary (citable) accession number: O43007
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.