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Protein

C-1-tetrahydrofolate synthase, mitochondrial

Gene

ade9

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei227NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi202 – 204NADPBy similarity3
Nucleotide bindingi405 – 412ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ligase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-196757 Metabolism of folate and pterines
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
C-1-tetrahydrofolate synthase, mitochondrial
Short name:
C1-THF synthase
Including the following 3 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Formyltetrahydrofolate synthetase (EC:6.3.4.3)
Gene namesi
Name:ade9
ORF Names:SPBC2G2.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC2G2.08
PomBaseiSPBC2G2.08 ade9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55MitochondrionSequence analysisAdd BLAST55
ChainiPRO_000031562756 – 972C-1-tetrahydrofolate synthase, mitochondrialAdd BLAST917

Proteomic databases

MaxQBiO43007
PaxDbiO43007
PRIDEiO43007

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi276831, 1 interactor
STRINGi4896.SPBC2G2.08.1

Structurei

3D structure databases

SMRiO43007
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 340Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd BLAST285
Regioni84 – 88Substrate bindingBy similarity5
Regioni131 – 133Substrate bindingBy similarity3
Regioni299 – 303Substrate bindingBy similarity5
Regioni341 – 972Formyltetrahydrofolate synthetaseAdd BLAST632

Domaini

This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000040280
InParanoidiO43007
KOiK00288
OrthoDBiEOG092C0B4M

Family and domain databases

CDDicd00477 FTHFS, 1 hit
HAMAPiMF_01543 FTHFS, 1 hit
MF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR000559 Formate_THF_ligase
IPR020628 Formate_THF_ligase_CS
IPR036291 NAD(P)-bd_dom_sf
IPR027417 P-loop_NTPase
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PfamiView protein in Pfam
PF01268 FTHFS, 1 hit
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00721 FTHFS_1, 1 hit
PS00767 THF_DHG_CYH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVMVSFNQL RNYFLESNSL RPSKWLFQSY GTSSSANILN GKLLARKLQR
60 70 80 90 100
SVAEEVQALK AKDRNFKPAL AIVQVGKRED SNVYVRMKEK AARLVGIDFK
110 120 130 140 150
YCPFPETIQM PALLHELKKL NDDHTVHGVL VQLPLPKHLN ERTVTESITP
160 170 180 190 200
PKDVDGFGAF NIGLLAKNDA TPIHYPCTPK GIMELLKDNK ISVAGLNAVV
210 220 230 240 250
LGRSDIVGNP ISYLLRKDNA TVTVCHSKTK DLIQHISNAD LVIAALGKPE
260 270 280 290 300
FVRGEWLKPG SVVVDVGINA VQRNGKRVLV GDVHFESASK VASSITPVPG
310 320 330 340 350
GVGPMTVAML MENIVNAAKI ARTENIYRKI DLNPLELKKP VPSDIEIANS
360 370 380 390 400
QEPKLISNLA KEMGIYDTEL ENYGNYKAKV NLAVYERLKH RKDGNYVVVS
410 420 430 440 450
GITPTPFGEG KSTVVAGLVQ AMGHLGKLGI ACVRQPSQGP TFGVKGGAAG
460 470 480 490 500
GGYAQFIPMD DFNLHMTGDI HAVTAANNLL VAALETRMFH ENTQSDAALI
510 520 530 540 550
KRLIPVKNGR RVIPRGLIGR WNRICASHNM DPEDVNNASP ELLKEFVRLN
560 570 580 590 600
VDPDTIECNR VLDVNDRFLR SIEVGKASTE KGHVRKTSFD ISVASECMSI
610 620 630 640 650
LALSCDLNDM HSRLSRMVIA NDKYGNAITA GDLGVSGALT VLLKDAIKPN
660 670 680 690 700
LMQTLEGTPA FVHAGPFANI SIGASSIIAD KIALKLAGTE SFDRPEDAGY
710 720 730 740 750
VVTEAGFASD MGMEKFFNIK CRYSKLVPNT VVLVTTVKAL KLHGGGPKLK
760 770 780 790 800
PGAPIPEEYL VENLDLVKNG CSNMVKHIQN CHKFNIPVVV AINSYKTDSS
810 820 830 840 850
KEHEIIREAA LQAGAVDAVP SDHWAQGGKG AIELAKSVMT ACDQSSNSKF
860 870 880 890 900
RLLYDSETSI EDKVNVIAKE MYGANGVEFS SLAKERINTF IKQGFGNLPI
910 920 930 940 950
CMAKTQYSLS HNPEFRNVPK NFTVPIRDMR LNAGAGFIYP LAAEIQTIPG
960 970
LPTAPAYLNI DICENGEIVG LS
Length:972
Mass (Da):105,757
Last modified:October 3, 2012 - v3
Checksum:iA14DFE32C0106F20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA17888.3
PIRiT40147
RefSeqiNP_596437.2, NM_001022356.2

Genome annotation databases

EnsemblFungiiSPBC2G2.08.1; SPBC2G2.08.1:pep; SPBC2G2.08
GeneIDi2540300
KEGGispo:SPBC2G2.08

Similar proteinsi

Entry informationi

Entry nameiC1TM_SCHPO
AccessioniPrimary (citable) accession number: O43007
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2012
Last modified: March 28, 2018
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health