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Protein

Probable pre-mRNA-splicing factor ATP-dependent RNA helicase prp43

Gene

prp43

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi98 – 1058ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pre-mRNA-splicing factor ATP-dependent RNA helicase prp43 (EC:3.6.4.13)
Gene namesi
Name:prp43
ORF Names:SPBC16H5.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16H5.10c.
PomBaseiSPBC16H5.10c. prp43.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: UniProtKB
  • nucleus Source: PomBase
  • spliceosomal complex Source: PomBase
  • U2-type spliceosomal complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Probable pre-mRNA-splicing factor ATP-dependent RNA helicase prp43PRO_0000055144Add
BLAST

Proteomic databases

MaxQBiO42945.

Interactioni

Protein-protein interaction databases

BioGridi276595. 31 interactions.
IntActiO42945. 3 interactions.
MINTiMINT-4674188.

Structurei

3D structure databases

ProteinModelPortaliO42945.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 250166Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini275 – 455181Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi197 – 2004DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000175261.
InParanoidiO42945.
KOiK12820.
OMAiFKNEMQD.
OrthoDBiEOG7M98QW.
PhylomeDBiO42945.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O42945-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPAQKKLRQ ESKNPYLAHL NNGDDSEEVV SSKGLTRRAT TVAQAAKAEE
60 70 80 90 100
GPNNFFNDKP FSQNYFKILE TRRELPVYQQ REEFLKIYHE NQIIVFVGET
110 120 130 140 150
GSGKTTQIPQ FVLYDELPHL TNTQIACTQP RRVAAMSVAK RVADEMDVDL
160 170 180 190 200
GEEVGYNIRF EDCSGPNTLL KYMTDGMLLR EAMTDHMLSR YSCIILDEAH
210 220 230 240 250
ERTLATDILM GLMKRLATRR PDLKIIVMSA TLDAKKFQKY FFDAPLLAVP
260 270 280 290 300
GRTYPVEIYY TQEPERDYLE AALRTVLQIH VEEGPGDILV FLTGEEEIED
310 320 330 340 350
ACRKITLEAD DLVREGAAGP LKVYPLYGSL PPNQQQRIFE PTPEDTKSGY
360 370 380 390 400
GRKVVISTNI AETSLTIDGI VYVVDPGFSK QKIYNPRIRV ESLLVSPISK
410 420 430 440 450
ASAQQRAGRA GRTRPGKCFR LYTEEAFRKE LIEQTYPEIL RSNLSSTVLE
460 470 480 490 500
LKKLGIDDLV HFDYMDPPAP ETMMRALEEL NYLNCLDDNG DLTPLGRKAS
510 520 530 540 550
EFPLDPNLAV MLIRSPEFYC SNEVLSLTAL LSVPNVFVRP NSARKLADEM
560 570 580 590 600
RQQFTHPDGD HLTLLNVYHA YKSGEGTADW CWNHFLSHRA LISADNVRKQ
610 620 630 640 650
LRRTMERQEV ELISTPFDDK NYYVNIRRAL VSGFFMQVAK KSANGKNYVT
660 670 680 690 700
MKDNQVVSLH PSCGLSVTPE WVVYNEFVLT TKSFIRNVTA IRPEWLIELA
710 720 730
PNYYDLDDFD NNKEVKSALQ KVYQMAARSK KNARR
Length:735
Mass (Da):83,804
Last modified:June 1, 1998 - v1
Checksum:iDC2A25F145F5A5C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17908.1.
AB027819 Genomic DNA. Translation: BAA87123.1.
PIRiT39615.
RefSeqiNP_595937.1. NM_001021845.2.

Genome annotation databases

EnsemblFungiiSPBC16H5.10c.1; SPBC16H5.10c.1:pep; SPBC16H5.10c.
GeneIDi2540057.
KEGGispo:SPBC16H5.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA17908.1.
AB027819 Genomic DNA. Translation: BAA87123.1.
PIRiT39615.
RefSeqiNP_595937.1. NM_001021845.2.

3D structure databases

ProteinModelPortaliO42945.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276595. 31 interactions.
IntActiO42945. 3 interactions.
MINTiMINT-4674188.

Proteomic databases

MaxQBiO42945.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC16H5.10c.1; SPBC16H5.10c.1:pep; SPBC16H5.10c.
GeneIDi2540057.
KEGGispo:SPBC16H5.10c.

Organism-specific databases

EuPathDBiFungiDB:SPBC16H5.10c.
PomBaseiSPBC16H5.10c. prp43.

Phylogenomic databases

HOGENOMiHOG000175261.
InParanoidiO42945.
KOiK12820.
OMAiFKNEMQD.
OrthoDBiEOG7M98QW.
PhylomeDBiO42945.

Miscellaneous databases

NextBioi20801194.
PROiO42945.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 536-729, SUBCELLULAR LOCATION.
    Strain: ATCC 38364 / 968.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDHX15_SCHPO
AccessioniPrimary (citable) accession number: O42945
Secondary accession number(s): Q9USE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.